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The map label for this gene is thiN [H]

Identifier: 222151056

GI number: 222151056

Start: 877023

End: 877661

Strand: Direct

Name: thiN [H]

Synonym: MCCL_0807

Alternate gene names: 222151056

Gene position: 877023-877661 (Clockwise)

Preceding gene: 222151055

Following gene: 222151057

Centisome position: 41.72

GC content: 38.34

Gene sequence:

>639_bases
ATGAATCTTCATATATTATGTACGCAGCTGGAAGTAGATGAATCGATTTTTGAGAGGTATGGAAGTGACGACTGGATTGG
TGTAGATTATGGTGCATTGATGCTGATTGATCGTAACATTCGACCAATCGCAGCATTTGGAGATTTCGACTCTATTGATG
AGACAGAGAAATTACGTATAGAGTCTATTATCGATATCGATTATCTTGCTGCTGAAAAGAATGAAACAGATCTCGAGGTT
GCAATGCAATATGCGAAAGACTTAGAGTACAACAAAGTATTTATTCATGGTGCGACGGGTGGAAGACTCGATCATTTTCT
TGGAAACCTGCAGACACTATTACATCCAGAAATATTATTGTCGACAAGCGAATTTCATATCGTTAATGCGAATAACACGA
TTCAGGTACTTACTGCAGGCACACATATCATTGAACATGAACCTGGTAAAAAGTATATATCCTTTATTCCGGTGAATGAC
GGTGTAACCTTGACGCTTGAGGGATTTAAATATGATGTTGATCGCCTTGAAGTAAGTCTTGGAATTACAAGAACGGTTTC
GAATGAATTTGCTGAGCGTCGTGCACAAGTCACTGTAGAACAAGGGATGGTTTATTGTATACAAAGTACAGAGCAATAA

Upstream 100 bases:

>100_bases
TATGTAAAGCAGCCGGTGCTGATGTACTTGTAGCAGGCTCTTATTTCTTTAAGCATGAAGATTATAAAATACCAGTAAAG
CTATTGAAAGGAGATAGAGA

Downstream 100 bases:

>100_bases
AAAAAAGACCTTTCACACAAAGGTCTTTTTTGTATCTATATATTCAGTTAAACGCGAGTCACTTTACCTGATTTTAATGC
ACGAGCTGAAACCCATACAC

Product: hypothetical protein

Products: NA

Alternate protein names: TPK; Thiamine diphosphokinase [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MNLHILCTQLEVDESIFERYGSDDWIGVDYGALMLIDRNIRPIAAFGDFDSIDETEKLRIESIIDIDYLAAEKNETDLEV
AMQYAKDLEYNKVFIHGATGGRLDHFLGNLQTLLHPEILLSTSEFHIVNANNTIQVLTAGTHIIEHEPGKKYISFIPVND
GVTLTLEGFKYDVDRLEVSLGITRTVSNEFAERRAQVTVEQGMVYCIQSTEQ

Sequences:

>Translated_212_residues
MNLHILCTQLEVDESIFERYGSDDWIGVDYGALMLIDRNIRPIAAFGDFDSIDETEKLRIESIIDIDYLAAEKNETDLEV
AMQYAKDLEYNKVFIHGATGGRLDHFLGNLQTLLHPEILLSTSEFHIVNANNTIQVLTAGTHIIEHEPGKKYISFIPVND
GVTLTLEGFKYDVDRLEVSLGITRTVSNEFAERRAQVTVEQGMVYCIQSTEQ
>Mature_212_residues
MNLHILCTQLEVDESIFERYGSDDWIGVDYGALMLIDRNIRPIAAFGDFDSIDETEKLRIESIIDIDYLAAEKNETDLEV
AMQYAKDLEYNKVFIHGATGGRLDHFLGNLQTLLHPEILLSTSEFHIVNANNTIQVLTAGTHIIEHEPGKKYISFIPVND
GVTLTLEGFKYDVDRLEVSLGITRTVSNEFAERRAQVTVEQGMVYCIQSTEQ

Specific function: Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate [H]

COG id: COG1564

COG function: function code H; Thiamine pyrophosphokinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine pyrophosphokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006282
- InterPro:   IPR007373
- InterPro:   IPR007371 [H]

Pfam domain/function: PF04263 TPK_catalytic [H]

EC number: =2.7.6.2 [H]

Molecular weight: Translated: 23986; Mature: 23986

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHILCTQLEVDESIFERYGSDDWIGVDYGALMLIDRNIRPIAAFGDFDSIDETEKLRI
CCEEEEEEEEECCHHHHHHCCCCCEEEECCCEEEEEECCCCEEEEECCCCCCCCHHHEEE
ESIIDIDYLAAEKNETDLEVAMQYAKDLEYNKVFIHGATGGRLDHFLGNLQTLLHPEILL
EHEECEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
STSEFHIVNANNTIQVLTAGTHIIEHEPGKKYISFIPVNDGVTLTLEGFKYDVDRLEVSL
ECCCEEEECCCCEEEEEEECCEEEECCCCCCEEEEEECCCCEEEEECCCEECHHHEEEEE
GITRTVSNEFAERRAQVTVEQGMVYCIQSTEQ
CEEEHHHHHHHHHHHEEEHHHCEEEEEECCCC
>Mature Secondary Structure
MNLHILCTQLEVDESIFERYGSDDWIGVDYGALMLIDRNIRPIAAFGDFDSIDETEKLRI
CCEEEEEEEEECCHHHHHHCCCCCEEEECCCEEEEEECCCCEEEEECCCCCCCCHHHEEE
ESIIDIDYLAAEKNETDLEVAMQYAKDLEYNKVFIHGATGGRLDHFLGNLQTLLHPEILL
EHEECEEEEECCCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
STSEFHIVNANNTIQVLTAGTHIIEHEPGKKYISFIPVNDGVTLTLEGFKYDVDRLEVSL
ECCCEEEECCCCEEEEEEECCEEEECCCCCCEEEEEECCCCEEEEECCCEECHHHEEEEE
GITRTVSNEFAERRAQVTVEQGMVYCIQSTEQ
CEEEHHHHHHHHHHHEEEHHHCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377 [H]