Click here to switch to the map view.

The map label for this gene is murD

Identifier: 222151005

GI number: 222151005

Start: 813978

End: 815315

Strand: Direct

Name: murD

Synonym: MCCL_0755

Alternate gene names: 222151005

Gene position: 813978-815315 (Clockwise)

Preceding gene: 222151004

Following gene: 222151006

Centisome position: 38.72

GC content: 38.71

Gene sequence:

>1338_bases
ATGAAACACATACAAAAGTATCAAGGGAAAAAGGTGCTTGTTCTCGGGCTTGGAAAAAGTGGTTATGAAACAGCGAAACT
GCTGTATCGTCTTGGGGCACAAGTTACTGTCAATGATGGTAAGGATGTTTCGCAGACAGAGCAATATCGCGAACTGACAG
AGATGGGGATTACTGTTATCGGAGGTCATCATCCCCTTTCATTGCTTGAACATACGACACTCATCGTCAAGAATCCGGGT
ATTCCTTACACTTTATCGTTAATAGAACAGGCACAAACAATGAATATCCCTATTATTACTGAAGTAGAACTAGCTTATGA
AATTACTGAAAGCTCGATTCTCGGTATTACGGGTACAAATGGTAAGACGACTGTTACATCCCTCATTGGAGATATGTTTA
AAGCGCATGAACATGCAGGGTTATTATGTGGAAACATCGGTTTTGTAGCTTCGAAAGTTGCACAGAATGCAGGTGCTCAT
GATACACTTGTTATGGAATTATCATCGTTCCAGCTGATGGGAATTCAAGAATTCAGACCGCATATTGCGCTCATTACAAA
TATTTATTCTGCTCATCTGGATTATCATGGTAACCAGCAAAACTATATTGATGCAAAGATGCAAATTACTAAAAATCAGA
CTAGCGAAGATTACCTTATATTCAATGATAAGCAGCGTACATTGTTGCAGAACTATAATATAAAGTCTAAAGTCGTATAT
TTCTCTACCGATGAAAAAGTAGAGGGGGCATACATAGATCAAAATAAAGTTTATTTCTATGATGAATTTATCATTGATGT
GAAAGACATCGTTCTGCCAGGCGTACATAACCTTGAAAATGTGCTGGCTAGTATTGCTGCTGCAAAACTTGCGGGCATAG
ACAATATTCATATTTGTGATACATTAAAGACGTTTGAAGGCATTAAACATCGCCTGCAGTTTGTAACTGAGACAGATGGT
GTGAAATATTACAATGATTCTAAAGCGACCAATACATTAGCAACAAGCTTTGCGTTAGATTCATTTCATCAACCGACGCA
TTGGCTAGCAGGAGGACTAGATAGAGGGAATGGGTTTGAAGAATTAGACACACATATTGATCATGTAAAGCACATGTATA
TCTTTGGAGAAACAACTGAAAAACTTACAGCTTTTGCAGCACAACATCATATCCCTTATACGATATGCAAAGATGTGACA
GATGCAGTGCTTAAAACTGCATCATATGTTGAGCGCGGTGAGGTAGTGCTACTCAGTCCAGCGTGTGCCAGCTGGGATCA
ATATCCAACGTACGAAGTAAGAGGAGAACACTTTATCAGTCAGGTCAAATTAATTTAG

Upstream 100 bases:

>100_bases
CTTTGAGATGGTGGGCTGGAGTGAATGGAAGATTGTAACCGTGTTCTGGATTGTCGGTATATTAACTGGACTCATCGGAA
TTTATTTAGGAGTGAATTAG

Downstream 100 bases:

>100_bases
AGGTGATATGATGGAGGATAAAATTATTCATACACCACGTTTTGATGAACAAAGAAGAATGCGCAGAAAGAAACGTCAGA
GACTGCAGCTATTTATCTTC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MKHIQKYQGKKVLVLGLGKSGYETAKLLYRLGAQVTVNDGKDVSQTEQYRELTEMGITVIGGHHPLSLLEHTTLIVKNPG
IPYTLSLIEQAQTMNIPIITEVELAYEITESSILGITGTNGKTTVTSLIGDMFKAHEHAGLLCGNIGFVASKVAQNAGAH
DTLVMELSSFQLMGIQEFRPHIALITNIYSAHLDYHGNQQNYIDAKMQITKNQTSEDYLIFNDKQRTLLQNYNIKSKVVY
FSTDEKVEGAYIDQNKVYFYDEFIIDVKDIVLPGVHNLENVLASIAAAKLAGIDNIHICDTLKTFEGIKHRLQFVTETDG
VKYYNDSKATNTLATSFALDSFHQPTHWLAGGLDRGNGFEELDTHIDHVKHMYIFGETTEKLTAFAAQHHIPYTICKDVT
DAVLKTASYVERGEVVLLSPACASWDQYPTYEVRGEHFISQVKLI

Sequences:

>Translated_445_residues
MKHIQKYQGKKVLVLGLGKSGYETAKLLYRLGAQVTVNDGKDVSQTEQYRELTEMGITVIGGHHPLSLLEHTTLIVKNPG
IPYTLSLIEQAQTMNIPIITEVELAYEITESSILGITGTNGKTTVTSLIGDMFKAHEHAGLLCGNIGFVASKVAQNAGAH
DTLVMELSSFQLMGIQEFRPHIALITNIYSAHLDYHGNQQNYIDAKMQITKNQTSEDYLIFNDKQRTLLQNYNIKSKVVY
FSTDEKVEGAYIDQNKVYFYDEFIIDVKDIVLPGVHNLENVLASIAAAKLAGIDNIHICDTLKTFEGIKHRLQFVTETDG
VKYYNDSKATNTLATSFALDSFHQPTHWLAGGLDRGNGFEELDTHIDHVKHMYIFGETTEKLTAFAAQHHIPYTICKDVT
DAVLKTASYVERGEVVLLSPACASWDQYPTYEVRGEHFISQVKLI
>Mature_445_residues
MKHIQKYQGKKVLVLGLGKSGYETAKLLYRLGAQVTVNDGKDVSQTEQYRELTEMGITVIGGHHPLSLLEHTTLIVKNPG
IPYTLSLIEQAQTMNIPIITEVELAYEITESSILGITGTNGKTTVTSLIGDMFKAHEHAGLLCGNIGFVASKVAQNAGAH
DTLVMELSSFQLMGIQEFRPHIALITNIYSAHLDYHGNQQNYIDAKMQITKNQTSEDYLIFNDKQRTLLQNYNIKSKVVY
FSTDEKVEGAYIDQNKVYFYDEFIIDVKDIVLPGVHNLENVLASIAAAKLAGIDNIHICDTLKTFEGIKHRLQFVTETDG
VKYYNDSKATNTLATSFALDSFHQPTHWLAGGLDRGNGFEELDTHIDHVKHMYIFGETTEKLTAFAAQHHIPYTICKDVT
DAVLKTASYVERGEVVLLSPACASWDQYPTYEVRGEHFISQVKLI

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=452, Percent_Identity=31.1946902654867, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_MACCJ (B9EB51)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002560158.1
- ProteinModelPortal:   B9EB51
- GeneID:   7390706
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0755
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49723; Mature: 49723

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHIQKYQGKKVLVLGLGKSGYETAKLLYRLGAQVTVNDGKDVSQTEQYRELTEMGITVI
CCCCHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCEEEE
GGHHPLSLLEHTTLIVKNPGIPYTLSLIEQAQTMNIPIITEVELAYEITESSILGITGTN
ECCCCHHHHCCEEEEEECCCCCEEHHHHHHHHHCCCCEEEEEHEEEEECCCCEEEEECCC
GKTTVTSLIGDMFKAHEHAGLLCGNIGFVASKVAQNAGAHDTLVMELSSFQLMGIQEFRP
CCHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCEEEEEECCCEEECHHHHCC
HIALITNIYSAHLDYHGNQQNYIDAKMQITKNQTSEDYLIFNDKQRTLLQNYNIKSKVVY
HHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCHHHHHHCCCCCEEEEE
FSTDEKVEGAYIDQNKVYFYDEFIIDVKDIVLPGVHNLENVLASIAAAKLAGIDNIHICD
EECCCCCCCEEECCCEEEEEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEHH
TLKTFEGIKHRLQFVTETDGVKYYNDSKATNTLATSFALDSFHQPTHWLAGGLDRGNGFE
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHH
ELDTHIDHVKHMYIFGETTEKLTAFAAQHHIPYTICKDVTDAVLKTASYVERGEVVLLSP
HHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECC
ACASWDQYPTYEVRGEHFISQVKLI
CCCCCCCCCCEEECHHHHHHHHCCC
>Mature Secondary Structure
MKHIQKYQGKKVLVLGLGKSGYETAKLLYRLGAQVTVNDGKDVSQTEQYRELTEMGITVI
CCCCHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHCCEEEE
GGHHPLSLLEHTTLIVKNPGIPYTLSLIEQAQTMNIPIITEVELAYEITESSILGITGTN
ECCCCHHHHCCEEEEEECCCCCEEHHHHHHHHHCCCCEEEEEHEEEEECCCCEEEEECCC
GKTTVTSLIGDMFKAHEHAGLLCGNIGFVASKVAQNAGAHDTLVMELSSFQLMGIQEFRP
CCHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHCCCCCCEEEEEECCCEEECHHHHCC
HIALITNIYSAHLDYHGNQQNYIDAKMQITKNQTSEDYLIFNDKQRTLLQNYNIKSKVVY
HHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCCCEEEECCCHHHHHHCCCCCEEEEE
FSTDEKVEGAYIDQNKVYFYDEFIIDVKDIVLPGVHNLENVLASIAAAKLAGIDNIHICD
EECCCCCCCEEECCCEEEEEEEEEEEHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEHH
TLKTFEGIKHRLQFVTETDGVKYYNDSKATNTLATSFALDSFHQPTHWLAGGLDRGNGFE
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHH
ELDTHIDHVKHMYIFGETTEKLTAFAAQHHIPYTICKDVTDAVLKTASYVERGEVVLLSP
HHHHHHHHHEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECC
ACASWDQYPTYEVRGEHFISQVKLI
CCCCCCCCCCEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA