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The map label for this gene is pbpA [H]

Identifier: 222151003

GI number: 222151003

Start: 810723

End: 812972

Strand: Direct

Name: pbpA [H]

Synonym: MCCL_0753

Alternate gene names: 222151003

Gene position: 810723-812972 (Clockwise)

Preceding gene: 222151002

Following gene: 222151004

Centisome position: 38.56

GC content: 40.09

Gene sequence:

>2250_bases
ATGTCACGAAGTAAACTAAAACTTAAGAAAAACAAAATAGGAGCAGTCCTCTTGATCATGTTATTCGGACTGCTCTTTTT
TTCATTGATTTTTAAATATGGCATGATCATGCTTACAGGAAAGTCTAGTGGCCAGGATCTTGAAATGCGTGCGAGCGAGA
AATATGTTCGGAATATTGTCAATCAGCCTGAACGTGGTAAGATTCTAGATCGCAATGGACAAGTACTTGCAGAGGATATT
GAATCTTATAAACTTGTCGCCATCCTCGATAAACGATTTTCGGAAGGGAGTAAAAAACCGAGACATGTCGTTAATAAGAA
GAAGACAGCTAAAGCTTTAGCTGACATCATTGATATGGATGAAAAGGATATTTATAAAGTGCTGAGCAATAAAAAAGCAT
TTCAGGTCGAATTCGGTAAGGCGGGTAAAGACTTGACGTTTGAGCAGAAACAGAAGATACAGAACCTGAAGATGCCTGGA
CTCACATTTTTCTCTGAGAAGAAAAGATTTTATCCAAATGGAAATTTCGCTTCACATTTAATTGGTTTTGCAGAAAAAAA
TGGGGATACAAACGTAATGACTGGGATGCTCGGTAGTGAGAAGATATTTGATTCTTATCTATCGGGAAAACCAGGTAAAA
CTACGTTTAAACAGGATATATGGAACTATGTACTGCCCAAGTCAGGCAATGTCGTGCCAGCACAGGACGGGGATGATGTT
ACGCTGACAATTGATAAGAACATTCAGATCTTTGTGGAAGACTCGCTCGATATGATGGTCAAACGCTATAAACCGAAAGA
CATCTTTGCTGTCGTGATGGATGCAAAGACGGGAGAAATATTAGGAAGTAGTCAACGACCAACGTTTAATCCTCAGACAA
GAGAAGGCTTCGGAGAAAAATGGGCGAACGATCTGTACCAGAATACATATGAGCCAGGTTCTACTTTTAAGACATTCGGT
CTTGCAGCAGCAATTGAAGAAAATCAATATGACCCAAAAGCAAAGTATGAATCAGGTGAGCGAGAAATAGACGGCATAAC
GATTTCTGACTGGAATGACGTTGGGTGGGGAACAATCACGATGAGCAAAGGGTTCCAATTGTCGTCAAATGTGCTGATGA
TGAAACTGCAAGATAAGGTAGGCATCGACAAGATGAAGACATATTATGAAAAGTTTGGTTTTGGCAGATCGACGCAATCA
CTCTTTGATTCAGAAGCTAGCGGACATATTTCGTGGAATGATGAACTGAGTCAAAAAGTAAGCTCATTCGGACAGTCTAC
AACCGTAACTCCGGCTCAGATGTTACAGGCTGAGAGTGCAATCGTCAATGAAGGTAAGATGCTGAAGCCCTACTTTGTGA
AGTCCATTAAGAGTCAGGATAATAATAATATTTATACAGGTAAGAAAGATATTATTGGACAACCGATTTCAAAATCGACA
GCACAAAAAACGATGCAGCAGCTCTACGATGTTGTAAATGGTAAGGAGATGCATGCGATTAACTATGGTCTAAAGGATTA
TAAAGTTGCTGGTAAGACTGGTACAGCACAAGTGCCGGATACGGAAAAAGGCGGCTACGTCCAAGGGGCTAATCCATATA
TGGTCAGCTTTATGGGGTATGCACCAGCCAAAGACCCTGAAGTTATCATCTACTACGGTATGAGTCTCGCACAGAAGAAT
GATGCTGAAGCATATGCACAAGGGGTTTCAAAAGGGTATACACCCCTTATGGAAAACACATTAAAATATTTGAATGTAGG
ATCACCTGACAAGGATAATGCAAAGCTTATATTTAATACACCGGATGTCATCGGAAGCAAGGATGATAAGGCATCTACTG
AGATTGAAAATGCTTCGCTTAAACCTGTTCGAATTGGAAACGGCCGTACCGTGACGAACCAGTTGCCTGCTAAAGGAACG
AAGTTATTAGCTGATGACAAAGTATTTATTCTGACTGAAGGGGCAAAAGTGATGCCGGATATTACTGGATGGAGTAAGCG
TGATCTCCTGATGTTCAGCACGTTGACCGGAATAGAGATACAGTTCAAAGGTAACGGTTACGCCATCAATCAGTCTATTG
ATGTAAATAATCCAATAAAGCAAGGTGATAAGCTGACAGTTGAACTTGATTCATTGGACCCGCTAAAACAATCTCCAGTC
TACAATGATGTTATTAAAGCGCAGCTGCAACAGGAAAAAGAAGTGTTAAAAGAACAGATAAAAGATGAAAAACAAAAAAA
TTCGAATTAA

Upstream 100 bases:

>100_bases
TCTGAAGTGATGAAACAGTCTTCATACGAACGTGTCTATAGTAAAGCGAAATCATACGGATTAAGCTTGAAAAATGATAA
CGTAAAGGTAGTGCAAAAAG

Downstream 100 bases:

>100_bases
AAGAAAGGAAATCAGTAATTCTGATATGTGAAAATTATGAATGAAATTTATTTTGGAGTCATTGCATTTATACTTACCGC
AATACTTGTCCCTTTGTTTA

Product: penicillin-binding protein 1

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 749; Mature: 748

Protein sequence:

>749_residues
MSRSKLKLKKNKIGAVLLIMLFGLLFFSLIFKYGMIMLTGKSSGQDLEMRASEKYVRNIVNQPERGKILDRNGQVLAEDI
ESYKLVAILDKRFSEGSKKPRHVVNKKKTAKALADIIDMDEKDIYKVLSNKKAFQVEFGKAGKDLTFEQKQKIQNLKMPG
LTFFSEKKRFYPNGNFASHLIGFAEKNGDTNVMTGMLGSEKIFDSYLSGKPGKTTFKQDIWNYVLPKSGNVVPAQDGDDV
TLTIDKNIQIFVEDSLDMMVKRYKPKDIFAVVMDAKTGEILGSSQRPTFNPQTREGFGEKWANDLYQNTYEPGSTFKTFG
LAAAIEENQYDPKAKYESGEREIDGITISDWNDVGWGTITMSKGFQLSSNVLMMKLQDKVGIDKMKTYYEKFGFGRSTQS
LFDSEASGHISWNDELSQKVSSFGQSTTVTPAQMLQAESAIVNEGKMLKPYFVKSIKSQDNNNIYTGKKDIIGQPISKST
AQKTMQQLYDVVNGKEMHAINYGLKDYKVAGKTGTAQVPDTEKGGYVQGANPYMVSFMGYAPAKDPEVIIYYGMSLAQKN
DAEAYAQGVSKGYTPLMENTLKYLNVGSPDKDNAKLIFNTPDVIGSKDDKASTEIENASLKPVRIGNGRTVTNQLPAKGT
KLLADDKVFILTEGAKVMPDITGWSKRDLLMFSTLTGIEIQFKGNGYAINQSIDVNNPIKQGDKLTVELDSLDPLKQSPV
YNDVIKAQLQQEKEVLKEQIKDEKQKNSN

Sequences:

>Translated_749_residues
MSRSKLKLKKNKIGAVLLIMLFGLLFFSLIFKYGMIMLTGKSSGQDLEMRASEKYVRNIVNQPERGKILDRNGQVLAEDI
ESYKLVAILDKRFSEGSKKPRHVVNKKKTAKALADIIDMDEKDIYKVLSNKKAFQVEFGKAGKDLTFEQKQKIQNLKMPG
LTFFSEKKRFYPNGNFASHLIGFAEKNGDTNVMTGMLGSEKIFDSYLSGKPGKTTFKQDIWNYVLPKSGNVVPAQDGDDV
TLTIDKNIQIFVEDSLDMMVKRYKPKDIFAVVMDAKTGEILGSSQRPTFNPQTREGFGEKWANDLYQNTYEPGSTFKTFG
LAAAIEENQYDPKAKYESGEREIDGITISDWNDVGWGTITMSKGFQLSSNVLMMKLQDKVGIDKMKTYYEKFGFGRSTQS
LFDSEASGHISWNDELSQKVSSFGQSTTVTPAQMLQAESAIVNEGKMLKPYFVKSIKSQDNNNIYTGKKDIIGQPISKST
AQKTMQQLYDVVNGKEMHAINYGLKDYKVAGKTGTAQVPDTEKGGYVQGANPYMVSFMGYAPAKDPEVIIYYGMSLAQKN
DAEAYAQGVSKGYTPLMENTLKYLNVGSPDKDNAKLIFNTPDVIGSKDDKASTEIENASLKPVRIGNGRTVTNQLPAKGT
KLLADDKVFILTEGAKVMPDITGWSKRDLLMFSTLTGIEIQFKGNGYAINQSIDVNNPIKQGDKLTVELDSLDPLKQSPV
YNDVIKAQLQQEKEVLKEQIKDEKQKNSN
>Mature_748_residues
SRSKLKLKKNKIGAVLLIMLFGLLFFSLIFKYGMIMLTGKSSGQDLEMRASEKYVRNIVNQPERGKILDRNGQVLAEDIE
SYKLVAILDKRFSEGSKKPRHVVNKKKTAKALADIIDMDEKDIYKVLSNKKAFQVEFGKAGKDLTFEQKQKIQNLKMPGL
TFFSEKKRFYPNGNFASHLIGFAEKNGDTNVMTGMLGSEKIFDSYLSGKPGKTTFKQDIWNYVLPKSGNVVPAQDGDDVT
LTIDKNIQIFVEDSLDMMVKRYKPKDIFAVVMDAKTGEILGSSQRPTFNPQTREGFGEKWANDLYQNTYEPGSTFKTFGL
AAAIEENQYDPKAKYESGEREIDGITISDWNDVGWGTITMSKGFQLSSNVLMMKLQDKVGIDKMKTYYEKFGFGRSTQSL
FDSEASGHISWNDELSQKVSSFGQSTTVTPAQMLQAESAIVNEGKMLKPYFVKSIKSQDNNNIYTGKKDIIGQPISKSTA
QKTMQQLYDVVNGKEMHAINYGLKDYKVAGKTGTAQVPDTEKGGYVQGANPYMVSFMGYAPAKDPEVIIYYGMSLAQKND
AEAYAQGVSKGYTPLMENTLKYLNVGSPDKDNAKLIFNTPDVIGSKDDKASTEIENASLKPVRIGNGRTVTNQLPAKGTK
LLADDKVFILTEGAKVMPDITGWSKRDLLMFSTLTGIEIQFKGNGYAINQSIDVNNPIKQGDKLTVELDSLDPLKQSPVY
NDVIKAQLQQEKEVLKEQIKDEKQKNSN

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=584, Percent_Identity=25.513698630137, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI1786854, Length=572, Percent_Identity=23.0769230769231, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 83585; Mature: 83454

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSKLKLKKNKIGAVLLIMLFGLLFFSLIFKYGMIMLTGKSSGQDLEMRASEKYVRNIV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHH
NQPERGKILDRNGQVLAEDIESYKLVAILDKRFSEGSKKPRHVVNKKKTAKALADIIDMD
CCCCCCCEECCCCCCHHHHHHCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
EKDIYKVLSNKKAFQVEFGKAGKDLTFEQKQKIQNLKMPGLTFFSEKKRFYPNGNFASHL
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHH
IGFAEKNGDTNVMTGMLGSEKIFDSYLSGKPGKTTFKQDIWNYVLPKSGNVVPAQDGDDV
HHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCE
TLTIDKNIQIFVEDSLDMMVKRYKPKDIFAVVMDAKTGEILGSSQRPTFNPQTREGFGEK
EEEECCCEEEEEECCHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCCCCCHHHHHHHH
WANDLYQNTYEPGSTFKTFGLAAAIEENQYDPKAKYESGEREIDGITISDWNDVGWGTIT
HHHHHHHCCCCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEE
MSKGFQLSSNVLMMKLQDKVGIDKMKTYYEKFGFGRSTQSLFDSEASGHISWNDELSQKV
ECCCCEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEECHHHHHHH
SSFGQSTTVTPAQMLQAESAIVNEGKMLKPYFVKSIKSQDNNNIYTGKKDIIGQPISKST
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCCHHHCCCCCCHHH
AQKTMQQLYDVVNGKEMHAINYGLKDYKVAGKTGTAQVPDTEKGGYVQGANPYMVSFMGY
HHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEECCCCEEEEEECC
APAKDPEVIIYYGMSLAQKNDAEAYAQGVSKGYTPLMENTLKYLNVGSPDKDNAKLIFNT
CCCCCCCEEEEECCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
PDVIGSKDDKASTEIENASLKPVRIGNGRTVTNQLPAKGTKLLADDKVFILTEGAKVMPD
CCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCEEEECCEEEEEECCCEECCC
ITGWSKRDLLMFSTLTGIEIQFKGNGYAINQSIDVNNPIKQGDKLTVELDSLDPLKQSPV
CCCCCCCCEEEEEEECCEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCC
YNDVIKAQLQQEKEVLKEQIKDEKQKNSN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SRSKLKLKKNKIGAVLLIMLFGLLFFSLIFKYGMIMLTGKSSGQDLEMRASEKYVRNIV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHH
NQPERGKILDRNGQVLAEDIESYKLVAILDKRFSEGSKKPRHVVNKKKTAKALADIIDMD
CCCCCCCEECCCCCCHHHHHHCEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
EKDIYKVLSNKKAFQVEFGKAGKDLTFEQKQKIQNLKMPGLTFFSEKKRFYPNGNFASHL
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHH
IGFAEKNGDTNVMTGMLGSEKIFDSYLSGKPGKTTFKQDIWNYVLPKSGNVVPAQDGDDV
HHHHHCCCCCCEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCEEECCCCCCE
TLTIDKNIQIFVEDSLDMMVKRYKPKDIFAVVMDAKTGEILGSSQRPTFNPQTREGFGEK
EEEECCCEEEEEECCHHHHHHHCCCCCEEEEEEECCCCHHCCCCCCCCCCCCHHHHHHHH
WANDLYQNTYEPGSTFKTFGLAAAIEENQYDPKAKYESGEREIDGITISDWNDVGWGTIT
HHHHHHHCCCCCCCCHHHHHHHEEECCCCCCCCCCCCCCCCEECCEEEECCCCCCCEEEE
MSKGFQLSSNVLMMKLQDKVGIDKMKTYYEKFGFGRSTQSLFDSEASGHISWNDELSQKV
ECCCCEECCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCEEECHHHHHHH
SSFGQSTTVTPAQMLQAESAIVNEGKMLKPYFVKSIKSQDNNNIYTGKKDIIGQPISKST
HHCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCEECCCHHHCCCCCCHHH
AQKTMQQLYDVVNGKEMHAINYGLKDYKVAGKTGTAQVPDTEKGGYVQGANPYMVSFMGY
HHHHHHHHHHHHCCCEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEECCCCEEEEEECC
APAKDPEVIIYYGMSLAQKNDAEAYAQGVSKGYTPLMENTLKYLNVGSPDKDNAKLIFNT
CCCCCCCEEEEECCHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEC
PDVIGSKDDKASTEIENASLKPVRIGNGRTVTNQLPAKGTKLLADDKVFILTEGAKVMPD
CCCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCEEEECCEEEEEECCCEECCC
ITGWSKRDLLMFSTLTGIEIQFKGNGYAINQSIDVNNPIKQGDKLTVELDSLDPLKQSPV
CCCCCCCCEEEEEEECCEEEEECCCCEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCC
YNDVIKAQLQQEKEVLKEQIKDEKQKNSN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]