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The map label for this gene is lacA [C]

Identifier: 222150923

GI number: 222150923

Start: 726773

End: 727321

Strand: Direct

Name: lacA [C]

Synonym: MCCL_0673

Alternate gene names: 222150923

Gene position: 726773-727321 (Clockwise)

Preceding gene: 222150922

Following gene: 222150924

Centisome position: 34.57

GC content: 26.05

Gene sequence:

>549_bases
GTGAAAAACATGTTTAGCATACTTAGATTATTTTCAAAAATATTATCAGTTTTCCCAAATTCTTTTTTATATTTTTTATA
TGAAATAAATAGTTTTTCTGAAGCAAAAATTTTTATAGCGATTAGATATATTATAGTAGGTAGTATGGTTGAAGCTATAG
GAGATAATGTTTACATAGGTAAAAATGTAACCCTTAAAAATGTAGAAAAATTAACTTTAGGTAATAATATTAGTATTCAT
AATAACTGTTATATTGACTCACATGGTGAAATATCTATTGGTAATGATGTTTCGATAGCACATAATTCAACAATACTCTC
ATCATCACATACTTGGGAGGATTTAGAAAAACCTATAAAATATAATAACATTACAAGCTTACCAGTAATAATTTATGATA
ATGTTTGGATTGGTTGTGGCGTTAGAATTTTAATGGGTGTAACTATAAATTCAAGAAGCGTCGTTGCTGCAAATGCTATT
GTTAATAGAGATGTTATGAGTAATAATTTGGTTGGTGGAATACCTATAAAAATCATAAAGGAGATTTAA

Upstream 100 bases:

>100_bases
AATTATGGTACAGGGATCAGACATAGAGAAAAATTTGTATTTCTATTATATATAATACCTTTTTATATTTTGTCTGAAAA
AAGGAGAGTAAGGATAAAAA

Downstream 100 bases:

>100_bases
AAATGAAAATACTAGTTACAGGAATAGCTGGTTTTATTGGATCAAATTTAGCAAAAAAATTAAAAGAAAAAGGGCATGAA
GTATTTGGAATCGATAATTT

Product: hypothetical protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MKNMFSILRLFSKILSVFPNSFLYFLYEINSFSEAKIFIAIRYIIVGSMVEAIGDNVYIGKNVTLKNVEKLTLGNNISIH
NNCYIDSHGEISIGNDVSIAHNSTILSSSHTWEDLEKPIKYNNITSLPVIIYDNVWIGCGVRILMGVTINSRSVVAANAI
VNRDVMSNNLVGGIPIKIIKEI

Sequences:

>Translated_182_residues
MKNMFSILRLFSKILSVFPNSFLYFLYEINSFSEAKIFIAIRYIIVGSMVEAIGDNVYIGKNVTLKNVEKLTLGNNISIH
NNCYIDSHGEISIGNDVSIAHNSTILSSSHTWEDLEKPIKYNNITSLPVIIYDNVWIGCGVRILMGVTINSRSVVAANAI
VNRDVMSNNLVGGIPIKIIKEI
>Mature_182_residues
MKNMFSILRLFSKILSVFPNSFLYFLYEINSFSEAKIFIAIRYIIVGSMVEAIGDNVYIGKNVTLKNVEKLTLGNNISIH
NNCYIDSHGEISIGNDVSIAHNSTILSSSHTWEDLEKPIKYNNITSLPVIIYDNVWIGCGVRILMGVTINSRSVVAANAI
VNRDVMSNNLVGGIPIKIIKEI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=177, Percent_Identity=28.8135593220339, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1786664, Length=112, Percent_Identity=37.5, Blast_Score=61, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=157, Percent_Identity=31.8471337579618, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20269; Mature: 20269

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMFSILRLFSKILSVFPNSFLYFLYEINSFSEAKIFIAIRYIIVGSMVEAIGDNVYIG
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCEEEC
KNVTLKNVEKLTLGNNISIHNNCYIDSHGEISIGNDVSIAHNSTILSSSHTWEDLEKPIK
CCCCCCCCEEEECCCCEEECCCEEECCCCCEEECCCEEEEECCEEECCCCCHHHHHCCCC
YNNITSLPVIIYDNVWIGCGVRILMGVTINSRSVVAANAIVNRDVMSNNLVGGIPIKIIK
CCCCCCCEEEEECCEEECCCEEEEEEEEECCCCEEEEHHHHHHHHHCCCCCCCCCCEEEE
EI
CC
>Mature Secondary Structure
MKNMFSILRLFSKILSVFPNSFLYFLYEINSFSEAKIFIAIRYIIVGSMVEAIGDNVYIG
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHHCCCEEEC
KNVTLKNVEKLTLGNNISIHNNCYIDSHGEISIGNDVSIAHNSTILSSSHTWEDLEKPIK
CCCCCCCCEEEECCCCEEECCCEEECCCCCEEECCCEEEEECCEEECCCCCHHHHHCCCC
YNNITSLPVIIYDNVWIGCGVRILMGVTINSRSVVAANAIVNRDVMSNNLVGGIPIKIIK
CCCCCCCEEEEECCEEECCCEEEEEEEEECCCCEEEEHHHHHHHHHCCCCCCCCCCEEEE
EI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA