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The map label for this gene is dppE [H]

Identifier: 222150859

GI number: 222150859

Start: 653215

End: 654852

Strand: Direct

Name: dppE [H]

Synonym: MCCL_0609

Alternate gene names: 222150859

Gene position: 653215-654852 (Clockwise)

Preceding gene: 222150858

Following gene: 222150861

Centisome position: 31.07

GC content: 33.03

Gene sequence:

>1638_bases
ATGAAAAAAAGCATGTCGTTATTTTTAGTTTTAATCGTGTCTTTAGCTGGTTTTCTAGCAGCCTGTTCATCAGGAGGAAA
ATCATCTGAAAGTGGCGGCAAAGATGATCAAGTTTTGAACTTTGCTTCTGGTTCAGATATCCCATCTTTAGATTCATCGT
TAGCTACTGACCAAGTGTCATTCGATACTTTCAACCAAGTAATGGAAGGGCTATATACTTTAGATAAAGATGATAAAGCC
GTTCCTGGTGTTGCAGAAGGTGAACCTGAGAAATCTAAAGACGGAAAAACTTGGACGATTAAATTACGTAAAGATGCAAA
ATGGTCTAATGGAGATTCAGTAACTGCTAAAGATTTCGTCTATGGATGGCAACGTACTCTAGATCCAAAAACGGGTTCTG
AATATGCTTATATCATGTCTGATATTAAGAATGCTGAAAAAGTTAATACAGGTAAAGCACAACCTTCTGAATTAGGTGTT
AAAGCAATCGATGATAATACACTTGAAATTCAGTTAGAATCAAATGTTCCATACTTCCAGGAGTTATTAGCATTTGGTAC
GTTCTTACCACAAAATGAGAAATTCGTAAAAGAAAAAGGCGATAAATACGGTACTTCAGTTGAAAACACATTATTTAATG
GTCCGTTTGTAGTTAGCAAATGGGAAACTGAAAAAGCATACCAATTAAAGAAAAATGATAAGTATTGGGATAAAGATAAA
GTTAAATTAAGCACAATTAATTATCAAATCGTTAAAGAAGCACAGACTTCTCTAAATTTATTTGAAACTGGCAAGATTGA
TTCGGTTGGATTAACAGCTGAGAACGTAGATAAATATAAAGATGATTCTAAATTCCAGACTGAATTAGACGCTTCTACAT
ACTTCTTACGTTTAAACCAAAAGAACGAAGATCTTAAAAATAAAAATTTAAGATTAGCAATTTCTAAAGCTATTGATAAA
GAAAAATATGTTAAAACATTATTAAATAATGGTTCAGTAGCAATTGATACATTATCACCAAAAGACTTCACTAAAGGTCC
AGATGGAAAAGACTTCTTAGATGGAATCGATTCACCACTGAACTATGATGTTAAAGAAGCACAGAAATATTATGAAAAAG
CTAAAAAAGAGCTAGGAAAAGATAAATTTACATTTGAATATTTAACTTATGATGCAGATACTTCAAAAACAGACGCAGAA
TATGTTAAAGAACAAATTGAAAAGAATCTTCCAGGTGTTACTTTTGAAATCAAGCAACAACCATTCAAACAAAAATTATC
TTTAGAGTCTAAAGGTAACTATGATATCTCATTTGCAGGCTGGGGTCCTGACTATCCAGATCCAATGACATTCATCGATA
TGTTTGTAACGGATGGTGCACATAACCAAACTGCTTGGTCTAACGCACAATACGATAAATTAATTGATGATGCTAAAGGT
CCGTTATTAGACGATACACAAAAACGTTGGGAAGCTATGCAACAAGCTGAACAATTATTATTAGATGAAGCTGTAATTAC
ACCAATGTATCAAAGAGGAGCAGCACGTCTACGTCAACCTTACATCAAAAACTTAGTAACTCATAAGTTTGCTGGAGATA
CAACTTTAAAAGAGACTTATGTTGAAGGTAAGAAGTAA

Upstream 100 bases:

>100_bases
AAAAAGGTATTGCCTATATGAAATTATTAAAATATAATTAATACAAATCTTCTGAAAATTGAAACAGACGATTTAATAAA
TGTTGGGGGGAAAGATTTAT

Downstream 100 bases:

>100_bases
GGTAAATGAGCTATGAGGTAAAGGCAATGAGTCTTTAACTTATGATATACCCGAACATTTTTAGAAGACAATTGTTGTTT
TCATGAAATGTTCGGGTTTT

Product: oligopeptide ABC transporter OppA oligopeptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 545

Protein sequence:

>545_residues
MKKSMSLFLVLIVSLAGFLAACSSGGKSSESGGKDDQVLNFASGSDIPSLDSSLATDQVSFDTFNQVMEGLYTLDKDDKA
VPGVAEGEPEKSKDGKTWTIKLRKDAKWSNGDSVTAKDFVYGWQRTLDPKTGSEYAYIMSDIKNAEKVNTGKAQPSELGV
KAIDDNTLEIQLESNVPYFQELLAFGTFLPQNEKFVKEKGDKYGTSVENTLFNGPFVVSKWETEKAYQLKKNDKYWDKDK
VKLSTINYQIVKEAQTSLNLFETGKIDSVGLTAENVDKYKDDSKFQTELDASTYFLRLNQKNEDLKNKNLRLAISKAIDK
EKYVKTLLNNGSVAIDTLSPKDFTKGPDGKDFLDGIDSPLNYDVKEAQKYYEKAKKELGKDKFTFEYLTYDADTSKTDAE
YVKEQIEKNLPGVTFEIKQQPFKQKLSLESKGNYDISFAGWGPDYPDPMTFIDMFVTDGAHNQTAWSNAQYDKLIDDAKG
PLLDDTQKRWEAMQQAEQLLLDEAVITPMYQRGAARLRQPYIKNLVTHKFAGDTTLKETYVEGKK

Sequences:

>Translated_545_residues
MKKSMSLFLVLIVSLAGFLAACSSGGKSSESGGKDDQVLNFASGSDIPSLDSSLATDQVSFDTFNQVMEGLYTLDKDDKA
VPGVAEGEPEKSKDGKTWTIKLRKDAKWSNGDSVTAKDFVYGWQRTLDPKTGSEYAYIMSDIKNAEKVNTGKAQPSELGV
KAIDDNTLEIQLESNVPYFQELLAFGTFLPQNEKFVKEKGDKYGTSVENTLFNGPFVVSKWETEKAYQLKKNDKYWDKDK
VKLSTINYQIVKEAQTSLNLFETGKIDSVGLTAENVDKYKDDSKFQTELDASTYFLRLNQKNEDLKNKNLRLAISKAIDK
EKYVKTLLNNGSVAIDTLSPKDFTKGPDGKDFLDGIDSPLNYDVKEAQKYYEKAKKELGKDKFTFEYLTYDADTSKTDAE
YVKEQIEKNLPGVTFEIKQQPFKQKLSLESKGNYDISFAGWGPDYPDPMTFIDMFVTDGAHNQTAWSNAQYDKLIDDAKG
PLLDDTQKRWEAMQQAEQLLLDEAVITPMYQRGAARLRQPYIKNLVTHKFAGDTTLKETYVEGKK
>Mature_545_residues
MKKSMSLFLVLIVSLAGFLAACSSGGKSSESGGKDDQVLNFASGSDIPSLDSSLATDQVSFDTFNQVMEGLYTLDKDDKA
VPGVAEGEPEKSKDGKTWTIKLRKDAKWSNGDSVTAKDFVYGWQRTLDPKTGSEYAYIMSDIKNAEKVNTGKAQPSELGV
KAIDDNTLEIQLESNVPYFQELLAFGTFLPQNEKFVKEKGDKYGTSVENTLFNGPFVVSKWETEKAYQLKKNDKYWDKDK
VKLSTINYQIVKEAQTSLNLFETGKIDSVGLTAENVDKYKDDSKFQTELDASTYFLRLNQKNEDLKNKNLRLAISKAIDK
EKYVKTLLNNGSVAIDTLSPKDFTKGPDGKDFLDGIDSPLNYDVKEAQKYYEKAKKELGKDKFTFEYLTYDADTSKTDAE
YVKEQIEKNLPGVTFEIKQQPFKQKLSLESKGNYDISFAGWGPDYPDPMTFIDMFVTDGAHNQTAWSNAQYDKLIDDAKG
PLLDDTQKRWEAMQQAEQLLLDEAVITPMYQRGAARLRQPYIKNLVTHKFAGDTTLKETYVEGKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=557, Percent_Identity=28.7253141831239, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1789397, Length=544, Percent_Identity=27.7573529411765, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081878, Length=538, Percent_Identity=30.6691449814126, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1787762, Length=528, Percent_Identity=23.2954545454545, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789887, Length=546, Percent_Identity=22.5274725274725, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1789966, Length=457, Percent_Identity=23.1947483588621, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1787052, Length=322, Percent_Identity=23.6024844720497, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61343; Mature: 61343

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSMSLFLVLIVSLAGFLAACSSGGKSSESGGKDDQVLNFASGSDIPSLDSSLATDQVS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCC
FDTFNQVMEGLYTLDKDDKAVPGVAEGEPEKSKDGKTWTIKLRKDAKWSNGDSVTAKDFV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHH
YGWQRTLDPKTGSEYAYIMSDIKNAEKVNTGKAQPSELGVKAIDDNTLEIQLESNVPYFQ
HHHHHHCCCCCCCCHHHHHHHHCCHHHCCCCCCCHHHCCCEEECCCEEEEEECCCCCHHH
ELLAFGTFLPQNEKFVKEKGDKYGTSVENTLFNGPFVVSKWETEKAYQLKKNDKYWDKDK
HHHHHHCCCCCCHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCCC
VKLSTINYQIVKEAQTSLNLFETGKIDSVGLTAENVDKYKDDSKFQTELDASTYFLRLNQ
EEEEEEHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHCCCCHHHHHCCCCEEEEEECC
KNEDLKNKNLRLAISKAIDKEKYVKTLLNNGSVAIDTLSPKDFTKGPDGKDFLDGIDSPL
CCCCCCCCCEEEEHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCC
NYDVKEAQKYYEKAKKELGKDKFTFEYLTYDADTSKTDAEYVKEQIEKNLPGVTFEIKQQ
CCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCC
PFKQKLSLESKGNYDISFAGWGPDYPDPMTFIDMFVTDGAHNQTAWSNAQYDKLIDDAKG
CHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC
PLLDDTQKRWEAMQQAEQLLLDEAVITPMYQRGAARLRQPYIKNLVTHKFAGDTTLKETY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VEGKK
CCCCC
>Mature Secondary Structure
MKKSMSLFLVLIVSLAGFLAACSSGGKSSESGGKDDQVLNFASGSDIPSLDSSLATDQVS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHCCCC
FDTFNQVMEGLYTLDKDDKAVPGVAEGEPEKSKDGKTWTIKLRKDAKWSNGDSVTAKDFV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHH
YGWQRTLDPKTGSEYAYIMSDIKNAEKVNTGKAQPSELGVKAIDDNTLEIQLESNVPYFQ
HHHHHHCCCCCCCCHHHHHHHHCCHHHCCCCCCCHHHCCCEEECCCEEEEEECCCCCHHH
ELLAFGTFLPQNEKFVKEKGDKYGTSVENTLFNGPFVVSKWETEKAYQLKKNDKYWDKDK
HHHHHHCCCCCCHHHHHHCCCCCCCCHHHHCCCCCEEEEECCCCHHHHHCCCCCCCCCCC
VKLSTINYQIVKEAQTSLNLFETGKIDSVGLTAENVDKYKDDSKFQTELDASTYFLRLNQ
EEEEEEHHHHHHHHHHHCCHHCCCCCCCCCCCHHHHHHHCCCCHHHHHCCCCEEEEEECC
KNEDLKNKNLRLAISKAIDKEKYVKTLLNNGSVAIDTLSPKDFTKGPDGKDFLDGIDSPL
CCCCCCCCCEEEEHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCC
NYDVKEAQKYYEKAKKELGKDKFTFEYLTYDADTSKTDAEYVKEQIEKNLPGVTFEIKQQ
CCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEECCC
PFKQKLSLESKGNYDISFAGWGPDYPDPMTFIDMFVTDGAHNQTAWSNAQYDKLIDDAKG
CHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCC
PLLDDTQKRWEAMQQAEQLLLDEAVITPMYQRGAARLRQPYIKNLVTHKFAGDTTLKETY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
VEGKK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]