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The map label for this gene is lipA

Identifier: 222150803

GI number: 222150803

Start: 591410

End: 592324

Strand: Direct

Name: lipA

Synonym: MCCL_0553

Alternate gene names: 222150803

Gene position: 591410-592324 (Clockwise)

Preceding gene: 222150802

Following gene: 222150804

Centisome position: 28.13

GC content: 39.23

Gene sequence:

>915_bases
TTGGCTACAAAAAATGAAGAAATACTAAGAAAACCCGAATGGCTAAAGATAAAACTAAATACTAATAAAAGTTACACTGG
TCTAAAAAAGATGATGCGCGAGCACAATTTAAATACAGTATGTGAAGAAGCGAAGTGTCCGAATATCCATGAATGCTGGG
GTGAAAGAAAGACTGCGACAATTATGATATTAGGTGCAATCTGTACACGTGCTTGTCGTTTTTGTGCAGTTAAGACAGGG
TTACCGAATGAACTTGACTTAAACGAACCAGAGCGTGTTGCAGAATCTGTTCGTCTAATGAATCTAAAGCACGTCGTTAT
CACAGCAGTTGCTCGTGATGATCTAAAAGACGGCGGTGCACATGTTTATGCGGAAACAATTCGTAAAGTACGTGAAGTTA
ATCCATATACAACAATTGAAGTATTACCGTCTGATATGGGAGGCAGTATTGAGAACTGGGAAACATTGATGGCAGCAAAA
CCAGATATCTTAAACCATAATATTGAAACGGTAAGACGCCTTACGCCAAGAGTTCGTGCACGTGCCACGTATGACCGTTC
ACTTGAAGTATTACGACGTTCTAAAGAACTATATCCAGATATCCCGACGAAATCGAGTTTAATGGTTGGACTTGGAGAAA
CAACAGAAGAAATCTACGAAGTGATGGATGATTTGCGTGCAAATGACGTAGATATTATGACAATCGGTCAGTACCTTCAG
CCAAGCCGTAAACACCTTAAAGTCCAAAAATATTATACACCGCTTGAATTCGGTAAGTTACGTAAAGTAGCAATGGAAAA
AGGATTCAAACATTGTCAGGCAGGACCAATGGTGAGAAGTTCATATCATGCAGATGAACAAGTAAATGAAGCTGCGAAAG
AAAAACATCGTTTAGGTGAGCTTTCTAGTAAGTAG

Upstream 100 bases:

>100_bases
TAAAAAATTTATAATACACGTGCTGAGTTTATAAATTTGACAATAATGGCATCTTACATATATGTTATAATGAAATCATC
AGAATTTTGGAGGGATTAGT

Downstream 100 bases:

>100_bases
GTGCGCTATGATCATACAGACTGGACGACATTATTTTGAAGTCATTGAGAATTATCGAGAAGCATTCAATCAGGAAATGT
TTGACCATCGATATTCAGAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MATKNEEILRKPEWLKIKLNTNKSYTGLKKMMREHNLNTVCEEAKCPNIHECWGERKTATIMILGAICTRACRFCAVKTG
LPNELDLNEPERVAESVRLMNLKHVVITAVARDDLKDGGAHVYAETIRKVREVNPYTTIEVLPSDMGGSIENWETLMAAK
PDILNHNIETVRRLTPRVRARATYDRSLEVLRRSKELYPDIPTKSSLMVGLGETTEEIYEVMDDLRANDVDIMTIGQYLQ
PSRKHLKVQKYYTPLEFGKLRKVAMEKGFKHCQAGPMVRSSYHADEQVNEAAKEKHRLGELSSK

Sequences:

>Translated_304_residues
MATKNEEILRKPEWLKIKLNTNKSYTGLKKMMREHNLNTVCEEAKCPNIHECWGERKTATIMILGAICTRACRFCAVKTG
LPNELDLNEPERVAESVRLMNLKHVVITAVARDDLKDGGAHVYAETIRKVREVNPYTTIEVLPSDMGGSIENWETLMAAK
PDILNHNIETVRRLTPRVRARATYDRSLEVLRRSKELYPDIPTKSSLMVGLGETTEEIYEVMDDLRANDVDIMTIGQYLQ
PSRKHLKVQKYYTPLEFGKLRKVAMEKGFKHCQAGPMVRSSYHADEQVNEAAKEKHRLGELSSK
>Mature_303_residues
ATKNEEILRKPEWLKIKLNTNKSYTGLKKMMREHNLNTVCEEAKCPNIHECWGERKTATIMILGAICTRACRFCAVKTGL
PNELDLNEPERVAESVRLMNLKHVVITAVARDDLKDGGAHVYAETIRKVREVNPYTTIEVLPSDMGGSIENWETLMAAKP
DILNHNIETVRRLTPRVRARATYDRSLEVLRRSKELYPDIPTKSSLMVGLGETTEEIYEVMDDLRANDVDIMTIGQYLQP
SRKHLKVQKYYTPLEFGKLRKVAMEKGFKHCQAGPMVRSSYHADEQVNEAAKEKHRLGELSSK

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=299, Percent_Identity=46.4882943143813, Blast_Score=268, Evalue=3e-72,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=47.1774193548387, Blast_Score=233, Evalue=1e-61,
Organism=Escherichia coli, GI1786846, Length=289, Percent_Identity=52.5951557093426, Blast_Score=303, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=43.8356164383562, Blast_Score=234, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=299, Percent_Identity=45.819397993311, Blast_Score=271, Evalue=1e-73,
Organism=Drosophila melanogaster, GI221513272, Length=287, Percent_Identity=48.0836236933798, Blast_Score=265, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_MACCJ (B9EAJ9)

Other databases:

- EMBL:   AP009484
- RefSeq:   YP_002559956.1
- GeneID:   7389649
- GenomeReviews:   AP009484_GR
- KEGG:   mcl:MCCL_0553
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 34755; Mature: 34623

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKNEEILRKPEWLKIKLNTNKSYTGLKKMMREHNLNTVCEEAKCPNIHECWGERKTAT
CCCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHCCCCHHH
IMILGAICTRACRFCAVKTGLPNELDLNEPERVAESVRLMNLKHVVITAVARDDLKDGGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HVYAETIRKVREVNPYTTIEVLPSDMGGSIENWETLMAAKPDILNHNIETVRRLTPRVRA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHH
RATYDRSLEVLRRSKELYPDIPTKSSLMVGLGETTEEIYEVMDDLRANDVDIMTIGQYLQ
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHC
PSRKHLKVQKYYTPLEFGKLRKVAMEKGFKHCQAGPMVRSSYHADEQVNEAAKEKHRLGE
CHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHC
LSSK
CCCC
>Mature Secondary Structure 
ATKNEEILRKPEWLKIKLNTNKSYTGLKKMMREHNLNTVCEEAKCPNIHECWGERKTAT
CCCCHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCHHHHHHCCCCCHHHHHCCCCHHH
IMILGAICTRACRFCAVKTGLPNELDLNEPERVAESVRLMNLKHVVITAVARDDLKDGGA
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HVYAETIRKVREVNPYTTIEVLPSDMGGSIENWETLMAAKPDILNHNIETVRRLTPRVRA
HHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHH
RATYDRSLEVLRRSKELYPDIPTKSSLMVGLGETTEEIYEVMDDLRANDVDIMTIGQYLQ
HHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEECCHHHC
PSRKHLKVQKYYTPLEFGKLRKVAMEKGFKHCQAGPMVRSSYHADEQVNEAAKEKHRLGE
CHHHHEEHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHC
LSSK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA