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The map label for this gene is yvoF [H]

Identifier: 222150763

GI number: 222150763

Start: 545171

End: 545644

Strand: Direct

Name: yvoF [H]

Synonym: MCCL_0513

Alternate gene names: 222150763

Gene position: 545171-545644 (Clockwise)

Preceding gene: 222150762

Following gene: 222150764

Centisome position: 25.93

GC content: 39.45

Gene sequence:

>474_bases
ATGCGTCAGTTAAATCGTCATAGATTAGAGGGGCCTAACCCGTTATGGCAGATGTATAAGACGGTTTCATTCTTTAAGGT
TTGTCGTAACTTTGTTGTTATTGAGTTATGTCGCATTCTGCCTTCAGTAAGATGGAAACATTACTTGTTCAGTAAATGTC
TGAAGATGCAGCTCGGACATCATGTGTCATTTGCTTATAAGGCGATGCCTGATCTGATGTTTCCTGAACTGATAAAAATA
GGTGATAACTCAATCATTGGGTATAACGCAACGTTACTTGCACATGAATATTTGACAGACGAGTATCGCACTGGCAAGAT
TACGATTGGTAGAAATGTATTAATTGGTGCGAATGTCACAGTCTTGCCAGGTGTTACAATAGGCGATGGTGCGAAGGTTG
GCGCTATGACAGTTGTAACGAAAGATATTCCTGAGAATGCCTTTGTATACGGTAATCCGATGAATATAAAATAA

Upstream 100 bases:

>100_bases
TTGATTATGATTGCAGCGACTTTAATATGGCTTTATCGTAATTATAAATATCGATTGCCGCGTTATGGTGAAGTGAATGA
ACCTATAAGGAGTTCTGCTT

Downstream 100 bases:

>100_bases
TAACAAACGGAGTGTGAGACTGGACTGAGTAAGTGGTGACTCCGTTTTCCAGCAACTGGAAGATGAATATTTATCAAATA
GGAATAAAGAAGCAGAGTGA

Product: O-acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRQLNRHRLEGPNPLWQMYKTVSFFKVCRNFVVIELCRILPSVRWKHYLFSKCLKMQLGHHVSFAYKAMPDLMFPELIKI
GDNSIIGYNATLLAHEYLTDEYRTGKITIGRNVLIGANVTVLPGVTIGDGAKVGAMTVVTKDIPENAFVYGNPMNIK

Sequences:

>Translated_157_residues
MRQLNRHRLEGPNPLWQMYKTVSFFKVCRNFVVIELCRILPSVRWKHYLFSKCLKMQLGHHVSFAYKAMPDLMFPELIKI
GDNSIIGYNATLLAHEYLTDEYRTGKITIGRNVLIGANVTVLPGVTIGDGAKVGAMTVVTKDIPENAFVYGNPMNIK
>Mature_157_residues
MRQLNRHRLEGPNPLWQMYKTVSFFKVCRNFVVIELCRILPSVRWKHYLFSKCLKMQLGHHVSFAYKAMPDLMFPELIKI
GDNSIIGYNATLLAHEYLTDEYRTGKITIGRNVLIGANVTVLPGVTIGDGAKVGAMTVVTKDIPENAFVYGNPMNIK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=86, Percent_Identity=44.1860465116279, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 17830; Mature: 17830

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQLNRHRLEGPNPLWQMYKTVSFFKVCRNFVVIELCRILPSVRWKHYLFSKCLKMQLGH
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
HVSFAYKAMPDLMFPELIKIGDNSIIGYNATLLAHEYLTDEYRTGKITIGRNVLIGANVT
CHHHHHHHCCCHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCEEEECCEEEEECCEE
VLPGVTIGDGAKVGAMTVVTKDIPENAFVYGNPMNIK
EECCEEECCCCCCCEEEEEEECCCCCCEEECCCCCCC
>Mature Secondary Structure
MRQLNRHRLEGPNPLWQMYKTVSFFKVCRNFVVIELCRILPSVRWKHYLFSKCLKMQLGH
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
HVSFAYKAMPDLMFPELIKIGDNSIIGYNATLLAHEYLTDEYRTGKITIGRNVLIGANVT
CHHHHHHHCCCHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCEEEECCEEEEECCEE
VLPGVTIGDGAKVGAMTVVTKDIPENAFVYGNPMNIK
EECCEEECCCCCCCEEEEEEECCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]