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The map label for this gene is opuBB [H]

Identifier: 222150724

GI number: 222150724

Start: 502346

End: 503860

Strand: Direct

Name: opuBB [H]

Synonym: MCCL_0474

Alternate gene names: 222150724

Gene position: 502346-503860 (Clockwise)

Preceding gene: 222150723

Following gene: 222150725

Centisome position: 23.89

GC content: 38.09

Gene sequence:

>1515_bases
ATGAACACGCTATGGCAGACGTTTAGTGAACGTAGGTATGAATTATTACAGGCTTTGTTCGAACATATTCAGATTTCATT
TATTGCGTTATTAATAGCTACCTTAATCGCAATACCCATTGGAATCTATTTAACGCGTCATACGAAGGTCGCTGAGCCCA
TTATTAATGTGACAGCGGTATTGCAGACAATTCCATCTCTTGCATTGCTCGGGTTGATGATTCCATTATTCGGTATTGGT
CGAGTACCGGCAATTATAGCACTCGTCATTTATGCTTTACTGCCGATTCTGCGCAATACATATACAGGTATCAAAGAAGT
AGATCCTTCACTGAAAGAGGCAGCGAGCGGAATCGGCATGAATACATTCCGTCAGCTGACAAAGGTTGAAATTCCTTTAG
CGATGCCGGTTATTATGGCTGGTATCAGAACAGCGATGGTCCTGATTATCGGAACTGCGACACTGGCGGCATTTATAGGT
GCGGGTGGCTTAGGAGATTTGATTCTTCTTGGTATAGACCGTAATAATACAAGTATTCTTCTGTTAGGAGCAATTCCCGC
AGCACTCCTTGCGTTACTATTTGATTTTCTATTAAAACGCATGGAGCAGCTCAGCTATAGAAAGCTTACCTACGTTCTCG
GTACGATGCTGCTTATATTCTTGCTTGTAGCGGTGGGACCACTATTGTTTGGTAAAGAAGAGAAACTGAAATTTGCAGGT
AAACTTGGAACAGAACCAGAAATTATTACAAATATGTACAAATACGTCATTGAAGAGAAGACAGATGTCGCAGTTGAAGT
GTCACCAGGTATGGGTAAGACGACATTTTTATTCAATGCTTTGAAATCAGATAACATTGATGGTTACTTGGAGTTTACAG
GAACGGTGCTTGGAGAAATCACTAAAGAAGATCCTAAAGCTACAACAGAAAGTGAAGTTTATCAACAAGCGAATGAAAGT
TTGAAGAAAAAATATGATATGGCTTTACTACAACCAATGAAATACAACAACACATATGCACTAGCAGTAAAGAAAAGTTA
TGCTGAAGAACATAACTTAAAGACGATATCTGACCTTGAAAAGGTAAAAGATGATGTTAAGGTAGGGTTTACCTTAGAAT
TTAATGATAGAAATGATGGATATAAAGGAATCCAGAAGAAATATAATATCAAGTTTAACAACGTGAAGACGATGGAACCG
AAGATTCGTTATCAGGCAATTGATCAGAATAAGATAGATCTGATGGATGCCTATTCAACTGATGCTGAACTTAAGAAATA
TGATATGGTCGTGCTAAAGGATGACAAACATCTATTCCCACCGTATCAGGGTGCACCTTTATTGAAACAGAGTACAATCG
AGAAATATCCAGAAGTTGTCGATGCATTAAATGCGCTTTCGGGTAAGATTACAGATGAAGAGATGCAAAAGATGAACTAT
GACGTTGCATATAACAATAAGACACCAAAAAGTGTCGCAAAGGCATACTTAATAAAAGAGAAGATTATAAAATAA

Upstream 100 bases:

>100_bases
TCACTGAGCATGAATATATTGCTGTAATGGAAGATACAAGAAGAATCGGTGTTATATCACGCAATGACGTATTACGTCAA
CTGAGTAAGGAGGCGATGTA

Downstream 100 bases:

>100_bases
ATTAATTTGTTAAAAAGATAGCAAAAATTTGCTATCTTTTTTTATTTTCTCTATCATAATATAAAACGAACTGGAGGGTC
TAATATGAAACCACAACTAG

Product: glycine/betaine ABC transporter substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MNTLWQTFSERRYELLQALFEHIQISFIALLIATLIAIPIGIYLTRHTKVAEPIINVTAVLQTIPSLALLGLMIPLFGIG
RVPAIIALVIYALLPILRNTYTGIKEVDPSLKEAASGIGMNTFRQLTKVEIPLAMPVIMAGIRTAMVLIIGTATLAAFIG
AGGLGDLILLGIDRNNTSILLLGAIPAALLALLFDFLLKRMEQLSYRKLTYVLGTMLLIFLLVAVGPLLFGKEEKLKFAG
KLGTEPEIITNMYKYVIEEKTDVAVEVSPGMGKTTFLFNALKSDNIDGYLEFTGTVLGEITKEDPKATTESEVYQQANES
LKKKYDMALLQPMKYNNTYALAVKKSYAEEHNLKTISDLEKVKDDVKVGFTLEFNDRNDGYKGIQKKYNIKFNNVKTMEP
KIRYQAIDQNKIDLMDAYSTDAELKKYDMVVLKDDKHLFPPYQGAPLLKQSTIEKYPEVVDALNALSGKITDEEMQKMNY
DVAYNNKTPKSVAKAYLIKEKIIK

Sequences:

>Translated_504_residues
MNTLWQTFSERRYELLQALFEHIQISFIALLIATLIAIPIGIYLTRHTKVAEPIINVTAVLQTIPSLALLGLMIPLFGIG
RVPAIIALVIYALLPILRNTYTGIKEVDPSLKEAASGIGMNTFRQLTKVEIPLAMPVIMAGIRTAMVLIIGTATLAAFIG
AGGLGDLILLGIDRNNTSILLLGAIPAALLALLFDFLLKRMEQLSYRKLTYVLGTMLLIFLLVAVGPLLFGKEEKLKFAG
KLGTEPEIITNMYKYVIEEKTDVAVEVSPGMGKTTFLFNALKSDNIDGYLEFTGTVLGEITKEDPKATTESEVYQQANES
LKKKYDMALLQPMKYNNTYALAVKKSYAEEHNLKTISDLEKVKDDVKVGFTLEFNDRNDGYKGIQKKYNIKFNNVKTMEP
KIRYQAIDQNKIDLMDAYSTDAELKKYDMVVLKDDKHLFPPYQGAPLLKQSTIEKYPEVVDALNALSGKITDEEMQKMNY
DVAYNNKTPKSVAKAYLIKEKIIK
>Mature_504_residues
MNTLWQTFSERRYELLQALFEHIQISFIALLIATLIAIPIGIYLTRHTKVAEPIINVTAVLQTIPSLALLGLMIPLFGIG
RVPAIIALVIYALLPILRNTYTGIKEVDPSLKEAASGIGMNTFRQLTKVEIPLAMPVIMAGIRTAMVLIIGTATLAAFIG
AGGLGDLILLGIDRNNTSILLLGAIPAALLALLFDFLLKRMEQLSYRKLTYVLGTMLLIFLLVAVGPLLFGKEEKLKFAG
KLGTEPEIITNMYKYVIEEKTDVAVEVSPGMGKTTFLFNALKSDNIDGYLEFTGTVLGEITKEDPKATTESEVYQQANES
LKKKYDMALLQPMKYNNTYALAVKKSYAEEHNLKTISDLEKVKDDVKVGFTLEFNDRNDGYKGIQKKYNIKFNNVKTMEP
KIRYQAIDQNKIDLMDAYSTDAELKKYDMVVLKDDKHLFPPYQGAPLLKQSTIEKYPEVVDALNALSGKITDEEMQKMNY
DVAYNNKTPKSVAKAYLIKEKIIK

Specific function: Involved in a high affinity multicomponent binding- protein-dependent transport system for choline; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1732

COG function: function code M; Periplasmic glycine betaine/choline-binding (lipo)protein of an ABC-type transport system (osmoprotectant binding protein)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788449, Length=185, Percent_Identity=40.5405405405405, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788451, Length=196, Percent_Identity=38.265306122449, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789033, Length=176, Percent_Identity=35.2272727272727, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788452, Length=283, Percent_Identity=24.3816254416961, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI87081802, Length=149, Percent_Identity=33.5570469798658, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56305; Mature: 56305

Theoretical pI: Translated: 8.96; Mature: 8.96

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTLWQTFSERRYELLQALFEHIQISFIALLIATLIAIPIGIYLTRHTKVAEPIINVTAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
LQTIPSLALLGLMIPLFGIGRVPAIIALVIYALLPILRNTYTGIKEVDPSLKEAASGIGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCH
NTFRQLTKVEIPLAMPVIMAGIRTAMVLIIGTATLAAFIGAGGLGDLILLGIDRNNTSIL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
LLGAIPAALLALLFDFLLKRMEQLSYRKLTYVLGTMLLIFLLVAVGPLLFGKEEKLKFAG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KLGTEPEIITNMYKYVIEEKTDVAVEVSPGMGKTTFLFNALKSDNIDGYLEFTGTVLGEI
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHH
TKEDPKATTESEVYQQANESLKKKYDMALLQPMKYNNTYALAVKKSYAEEHNLKTISDLE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCHHHHHHH
KVKDDVKVGFTLEFNDRNDGYKGIQKKYNIKFNNVKTMEPKIRYQAIDQNKIDLMDAYST
HHHHHHEEEEEEEECCCCCCHHHHHHHCCEEECCEEECCCCHHEEECCCCCCCHHHCCCC
DAELKKYDMVVLKDDKHLFPPYQGAPLLKQSTIEKYPEVVDALNALSGKITDEEMQKMNY
CCHHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
DVAYNNKTPKSVAKAYLIKEKIIK
CEEECCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNTLWQTFSERRYELLQALFEHIQISFIALLIATLIAIPIGIYLTRHTKVAEPIINVTAV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHH
LQTIPSLALLGLMIPLFGIGRVPAIIALVIYALLPILRNTYTGIKEVDPSLKEAASGIGM
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHCCCCH
NTFRQLTKVEIPLAMPVIMAGIRTAMVLIIGTATLAAFIGAGGLGDLILLGIDRNNTSIL
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEE
LLGAIPAALLALLFDFLLKRMEQLSYRKLTYVLGTMLLIFLLVAVGPLLFGKEEKLKFAG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
KLGTEPEIITNMYKYVIEEKTDVAVEVSPGMGKTTFLFNALKSDNIDGYLEFTGTVLGEI
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCEEEEEHHHHHHHH
TKEDPKATTESEVYQQANESLKKKYDMALLQPMKYNNTYALAVKKSYAEEHNLKTISDLE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCHHHHHHH
KVKDDVKVGFTLEFNDRNDGYKGIQKKYNIKFNNVKTMEPKIRYQAIDQNKIDLMDAYST
HHHHHHEEEEEEEECCCCCCHHHHHHHCCEEECCEEECCCCHHEEECCCCCCCHHHCCCC
DAELKKYDMVVLKDDKHLFPPYQGAPLLKQSTIEKYPEVVDALNALSGKITDEEMQKMNY
CCHHHHEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
DVAYNNKTPKSVAKAYLIKEKIIK
CEEECCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7592481; 10216873; 9384377 [H]