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The map label for this gene is fruB [H]

Identifier: 222150704

GI number: 222150704

Start: 479753

End: 481642

Strand: Direct

Name: fruB [H]

Synonym: MCCL_0454

Alternate gene names: 222150704

Gene position: 479753-481642 (Clockwise)

Preceding gene: 222150703

Following gene: 222150705

Centisome position: 22.82

GC content: 40.58

Gene sequence:

>1890_bases
ATGAGAATCACTGATTTGCTTACATTTGATACGATGGCAATGGATTTACAAGCGACAACGAAAGACGGTGTGATTGATGA
GTTAGTCGAGACATTATCAGCTGCTGGTATATTATCAGACAAAGCATCATTTAAAGAAGCAATACACGCACGTGAAGCAC
AGAGTACTACAGGTGTTGGTGAAGGGATTGCAATTCCCCACGCGAAAAATGATGCGGTTAAGAAACCAGCTATAGCATTT
GGTAAAGCGGTAGATGGCATCGATTACGATTCACTTGATGGGCAACCAGCGCGCTTATTCTTTATGATTGCAGCACCTGC
AGGAGGTGCACAGACACATCTGGAGGCTTTAGCGAAGTTATCAGGTATATTAATGAATGAAGATGTTAGAACTGAATTAC
TACATGCTAAGACTAAAGAAGAAGTTATCGAAACAATTAACTTGCATGATGAAATTGAAAAAGATGAAGAAGTCGTATTG
GCACCTACTGGAGATGAGGATGATGATAACCTCGTGTTAGCTGTTACTGCATGTCCTACAGGAATCGCACATACGTATAT
GGCAGCAGATGCATTAAAGAATCAAGCTGTTAAGATGGGTGTGCCGATTAAAGTTGAGACAAATGGTTCAGGAGGCATTA
AAAACAGATTAACAGAGGAAGAAATTAAAAAAGCAAAAGGTATTATCGTTGCAGCAGACGTGAATGTTGAAACAAATCGT
TTCCATGGTAAGAATGTTGTGACAGTACCAGTAGCAGATGGCATTAAACGTCCTGAAGAGTTGATTAATATGGCTTTAGA
TACAACGAGACCAGCATATGTAGCAAAGGATGATAAAGGTACTACGAATTCCAGTGGAGAGCGTAAAGGATTTTATAAAC
ACTTGATGAGCGGTGTATCTAATATGCTACCTTTCGTTGTTGCAGGTGGGATATTAATGGCTATTGCATTTATGTTTGGC
ATTACATCGTTCGATCCGACGAAATCAGATTATCATCCTTTTGCAGAAGCGTTATGGGCGATTGGGAATAAGTCTGCATT
TGCCTTAATGGTTCCGATATTAGCAGGTTTCATCGCAATGAGTATTGCAGATCGTCCTGGATTGGCACCAGGTATGGTTG
GAGGTATGTTAGCTGTTATGGGCGGTTCAGGCTTCCTTGGTGGATTGATTGCCGGATTCTTAGCAGGTTATTTAGTAGAA
GCAATTAAGAAGATGTTAAGTGGCATGCCACAAGCATTAGATGGATTAAAACCGACGCTATTATATCCATTGCTTGGTGT
AACAAGCACAGGATTAATTATGTACTACATTATCAATCCACCAGCGAGCTGGTTAAATAAATTAATGGCAGATACTTTAA
ATAGTATGGATGGAACGAATATTGTATTACTTGGTTTAGTGTTAGGTGGTATGATGGCCATTGATATGGGTGGACCATTT
AATAAGGCATCATACGCATTCGGATTTGCAGCAATTGAAGCGGGGAACTACGCACCGATTACTGCTGCGATGATAGGTGG
AATGGTTCCACCATTAGCAATAGCGCTATCAATGATTCTATTTAAGAAAAAATATACTAAGACACAACGTGGCTCAATCG
TTTCAAATATCGTAATGGGAGCATCATTCATTACTGAAGGTGCAATTCCATTTGCAGCAGCAGATCCATTACGTGTCATT
CCATCAATGATTGCAGGAAGTGCAACTGCCGGAGCACTTGCAATGGCATTCGGATGTCGTGTTCCAGCACCGCATGGTGG
TATATTCGTAATTGGACTTGATAAAGCGCACTGGCCGATGTTCTTACTTGCATTGCTCATCGGTGTTGTCGTATCAGCAG
TCATTTACGGTTTAATTAAGAAACGTCCAGAAGAACTAGAAGTGAAATAA

Upstream 100 bases:

>100_bases
AGCGACAGCATTTACAGAAGACTTAGCAACAAATGAAGAAATTCAAGAGACAATACACCAAATACAAATTGAAGAGCTTT
AATAAATAGGAGGGAAAATA

Downstream 100 bases:

>100_bases
AGTATAAATTAAACCCTGCCGTACTAAAATAAAAGTACGGCAGGGTTTTTTGAAATATTTTCAATAGATTGACATATTAT
AAATCTTTTCTCATTATTTA

Product: fructose specific permease

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MRITDLLTFDTMAMDLQATTKDGVIDELVETLSAAGILSDKASFKEAIHAREAQSTTGVGEGIAIPHAKNDAVKKPAIAF
GKAVDGIDYDSLDGQPARLFFMIAAPAGGAQTHLEALAKLSGILMNEDVRTELLHAKTKEEVIETINLHDEIEKDEEVVL
APTGDEDDDNLVLAVTACPTGIAHTYMAADALKNQAVKMGVPIKVETNGSGGIKNRLTEEEIKKAKGIIVAADVNVETNR
FHGKNVVTVPVADGIKRPEELINMALDTTRPAYVAKDDKGTTNSSGERKGFYKHLMSGVSNMLPFVVAGGILMAIAFMFG
ITSFDPTKSDYHPFAEALWAIGNKSAFALMVPILAGFIAMSIADRPGLAPGMVGGMLAVMGGSGFLGGLIAGFLAGYLVE
AIKKMLSGMPQALDGLKPTLLYPLLGVTSTGLIMYYIINPPASWLNKLMADTLNSMDGTNIVLLGLVLGGMMAIDMGGPF
NKASYAFGFAAIEAGNYAPITAAMIGGMVPPLAIALSMILFKKKYTKTQRGSIVSNIVMGASFITEGAIPFAAADPLRVI
PSMIAGSATAGALAMAFGCRVPAPHGGIFVIGLDKAHWPMFLLALLIGVVVSAVIYGLIKKRPEELEVK

Sequences:

>Translated_629_residues
MRITDLLTFDTMAMDLQATTKDGVIDELVETLSAAGILSDKASFKEAIHAREAQSTTGVGEGIAIPHAKNDAVKKPAIAF
GKAVDGIDYDSLDGQPARLFFMIAAPAGGAQTHLEALAKLSGILMNEDVRTELLHAKTKEEVIETINLHDEIEKDEEVVL
APTGDEDDDNLVLAVTACPTGIAHTYMAADALKNQAVKMGVPIKVETNGSGGIKNRLTEEEIKKAKGIIVAADVNVETNR
FHGKNVVTVPVADGIKRPEELINMALDTTRPAYVAKDDKGTTNSSGERKGFYKHLMSGVSNMLPFVVAGGILMAIAFMFG
ITSFDPTKSDYHPFAEALWAIGNKSAFALMVPILAGFIAMSIADRPGLAPGMVGGMLAVMGGSGFLGGLIAGFLAGYLVE
AIKKMLSGMPQALDGLKPTLLYPLLGVTSTGLIMYYIINPPASWLNKLMADTLNSMDGTNIVLLGLVLGGMMAIDMGGPF
NKASYAFGFAAIEAGNYAPITAAMIGGMVPPLAIALSMILFKKKYTKTQRGSIVSNIVMGASFITEGAIPFAAADPLRVI
PSMIAGSATAGALAMAFGCRVPAPHGGIFVIGLDKAHWPMFLLALLIGVVVSAVIYGLIKKRPEELEVK
>Mature_629_residues
MRITDLLTFDTMAMDLQATTKDGVIDELVETLSAAGILSDKASFKEAIHAREAQSTTGVGEGIAIPHAKNDAVKKPAIAF
GKAVDGIDYDSLDGQPARLFFMIAAPAGGAQTHLEALAKLSGILMNEDVRTELLHAKTKEEVIETINLHDEIEKDEEVVL
APTGDEDDDNLVLAVTACPTGIAHTYMAADALKNQAVKMGVPIKVETNGSGGIKNRLTEEEIKKAKGIIVAADVNVETNR
FHGKNVVTVPVADGIKRPEELINMALDTTRPAYVAKDDKGTTNSSGERKGFYKHLMSGVSNMLPFVVAGGILMAIAFMFG
ITSFDPTKSDYHPFAEALWAIGNKSAFALMVPILAGFIAMSIADRPGLAPGMVGGMLAVMGGSGFLGGLIAGFLAGYLVE
AIKKMLSGMPQALDGLKPTLLYPLLGVTSTGLIMYYIINPPASWLNKLMADTLNSMDGTNIVLLGLVLGGMMAIDMGGPF
NKASYAFGFAAIEAGNYAPITAAMIGGMVPPLAIALSMILFKKKYTKTQRGSIVSNIVMGASFITEGAIPFAAADPLRVI
PSMIAGSATAGALAMAFGCRVPAPHGGIFVIGLDKAHWPMFLLALLIGVVVSAVIYGLIKKRPEELEVK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=456, Percent_Identity=47.3684210526316, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786951, Length=603, Percent_Identity=34.1625207296849, Blast_Score=339, Evalue=3e-94,
Organism=Escherichia coli, GI87082348, Length=474, Percent_Identity=34.1772151898734, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI1790386, Length=304, Percent_Identity=38.4868421052632, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788729, Length=389, Percent_Identity=26.2210796915167, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1790387, Length=93, Percent_Identity=44.0860215053763, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1788730, Length=86, Percent_Identity=45.3488372093023, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI2367327, Length=129, Percent_Identity=25.5813953488372, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789597, Length=122, Percent_Identity=31.9672131147541, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 66229; Mature: 66229

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2 ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITDLLTFDTMAMDLQATTKDGVIDELVETLSAAGILSDKASFKEAIHAREAQSTTGVG
CCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGIAIPHAKNDAVKKPAIAFGKAVDGIDYDSLDGQPARLFFMIAAPAGGAQTHLEALAKL
CCEECCCCCCCCCCCCHHHHHCHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
SGILMNEDVRTELLHAKTKEEVIETINLHDEIEKDEEVVLAPTGDEDDDNLVLAVTACPT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCC
GIAHTYMAADALKNQAVKMGVPIKVETNGSGGIKNRLTEEEIKKAKGIIVAADVNVETNR
CHHHHHHHHHHHHCCCEEECCCEEEEECCCCCHHHCCCHHHHHHHCCEEEEEECCEECCC
FHGKNVVTVPVADGIKRPEELINMALDTTRPAYVAKDDKGTTNSSGERKGFYKHLMSGVS
CCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
NMLPFVVAGGILMAIAFMFGITSFDPTKSDYHPFAEALWAIGNKSAFALMVPILAGFIAM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SIADRPGLAPGMVGGMLAVMGGSGFLGGLIAGFLAGYLVEAIKKMLSGMPQALDGLKPTL
HHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHH
LYPLLGVTSTGLIMYYIINPPASWLNKLMADTLNSMDGTNIVLLGLVLGGMMAIDMGGPF
HHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCC
NKASYAFGFAAIEAGNYAPITAAMIGGMVPPLAIALSMILFKKKYTKTQRGSIVSNIVMG
CCCHHHHEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ASFITEGAIPFAAADPLRVIPSMIAGSATAGALAMAFGCRVPAPHGGIFVIGLDKAHWPM
HHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHH
FLLALLIGVVVSAVIYGLIKKRPEELEVK
HHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure
MRITDLLTFDTMAMDLQATTKDGVIDELVETLSAAGILSDKASFKEAIHAREAQSTTGVG
CCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
EGIAIPHAKNDAVKKPAIAFGKAVDGIDYDSLDGQPARLFFMIAAPAGGAQTHLEALAKL
CCEECCCCCCCCCCCCHHHHHCHHCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHH
SGILMNEDVRTELLHAKTKEEVIETINLHDEIEKDEEVVLAPTGDEDDDNLVLAVTACPT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCEEEEEEECCC
GIAHTYMAADALKNQAVKMGVPIKVETNGSGGIKNRLTEEEIKKAKGIIVAADVNVETNR
CHHHHHHHHHHHHCCCEEECCCEEEEECCCCCHHHCCCHHHHHHHCCEEEEEECCEECCC
FHGKNVVTVPVADGIKRPEELINMALDTTRPAYVAKDDKGTTNSSGERKGFYKHLMSGVS
CCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHH
NMLPFVVAGGILMAIAFMFGITSFDPTKSDYHPFAEALWAIGNKSAFALMVPILAGFIAM
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHH
SIADRPGLAPGMVGGMLAVMGGSGFLGGLIAGFLAGYLVEAIKKMLSGMPQALDGLKPTL
HHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHH
LYPLLGVTSTGLIMYYIINPPASWLNKLMADTLNSMDGTNIVLLGLVLGGMMAIDMGGPF
HHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEECCCCC
NKASYAFGFAAIEAGNYAPITAAMIGGMVPPLAIALSMILFKKKYTKTQRGSIVSNIVMG
CCCHHHHEEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
ASFITEGAIPFAAADPLRVIPSMIAGSATAGALAMAFGCRVPAPHGGIFVIGLDKAHWPM
HHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCHHHH
FLLALLIGVVVSAVIYGLIKKRPEELEVK
HHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]