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The map label for this gene is pit [H]

Identifier: 222150653

GI number: 222150653

Start: 430801

End: 431811

Strand: Direct

Name: pit [H]

Synonym: MCCL_0403

Alternate gene names: 222150653

Gene position: 430801-431811 (Clockwise)

Preceding gene: 222150652

Following gene: 222150654

Centisome position: 20.49

GC content: 39.96

Gene sequence:

>1011_bases
ATGGAACCGATTATTATTTTTACATTTGCGATTGTATTCTTCGCGTTAGCTTTTGATTTTATCAACGGTTTTCACGATAC
GGCAAACGCAATAGCAACAGCAGTTTCAACGAAAGCACTGAAGCCACGTCATGCAATCTTGATGGCTGCTGTGCTTAACT
TTATCGGGGCGATTCTTTTTCATGGGGTAGCGAAGACGGTTACGAAAGATATTGTAGACCCGTTTACGTTACAAAATGGT
CAAGTGGTCATCCTTGCGGCATTAATCAGTGCGATTACATGGAACTTAATCACATGGTATTTTGGGATTCCGAGTTCGTC
ATCACATACATTGATTGGCTCAATTGCAGGTGCTGCAATTGCATCAGGTGGTTTTGGAATTCTGGAATATGCAGGATTTA
CGAAGATTATTGTAGGATTATTGATTTCACCATTACTTGCATTCATCGTTGGTTATATTATGTATACCATTATTAAAACA
ATATTTAAGAATGCAAACTTAACAAAGACAAATCGTAATTTTAGGTTCTTGCAAATATTCACAGCAGCAGCGCAAGCATT
TGCGCATGGTTCAAATGATGCGCAAAAGGCGATGGGTATTATTACGATGGCATTAATCTCTGCTGGGATGCAAACTGGAA
GTACCGAACCTCAGACATGGGTTCAAATCTCCTGTGCTGCTGCTATGGGATTAGGTACAGCAGTTGGTGGATGGAAGATC
ATTAAAACAGTTGGTGGTAATATCATGAAAATTCGTCCGGCCAATGGTGTTGCAGCAGACTTGTCATCTGCTTTTATCAT
CTTTGGAGCAACACATTTTCATCTACCTGTATCCACAACGCACGTTGTATCCTCTTCAATTCTAGGTGTCGGATCGAGTC
ACCGTGTAAAAGGCGTGAAGTGGACGACGGCACAGCGTATGATTATTACATGGGTGATTACATTGCCGATTTCAGCCTTA
ATTGCAGCAATTATCTATTTTGCTGTTAACTTTATTACACATATTTTTTAA

Upstream 100 bases:

>100_bases
AGGATATTTATCAGTCTTTAGAAGATATTGCTGATAAATGCCAGGCAGTTGCGAATATACTCGAATCTATCATTATGAAG
AATAGTTAAGGAGCATTTTT

Downstream 100 bases:

>100_bases
TTAAATCATATACATTCGGGTAAACTGTTAATAGTTTACCCGAATTTTTTTAGGAGTGATAAAGATGGAACATATTGTAT
TTGTACATAGTGCAAGTGAA

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MEPIIIFTFAIVFFALAFDFINGFHDTANAIATAVSTKALKPRHAILMAAVLNFIGAILFHGVAKTVTKDIVDPFTLQNG
QVVILAALISAITWNLITWYFGIPSSSSHTLIGSIAGAAIASGGFGILEYAGFTKIIVGLLISPLLAFIVGYIMYTIIKT
IFKNANLTKTNRNFRFLQIFTAAAQAFAHGSNDAQKAMGIITMALISAGMQTGSTEPQTWVQISCAAAMGLGTAVGGWKI
IKTVGGNIMKIRPANGVAADLSSAFIIFGATHFHLPVSTTHVVSSSILGVGSSHRVKGVKWTTAQRMIITWVITLPISAL
IAAIIYFAVNFITHIF

Sequences:

>Translated_336_residues
MEPIIIFTFAIVFFALAFDFINGFHDTANAIATAVSTKALKPRHAILMAAVLNFIGAILFHGVAKTVTKDIVDPFTLQNG
QVVILAALISAITWNLITWYFGIPSSSSHTLIGSIAGAAIASGGFGILEYAGFTKIIVGLLISPLLAFIVGYIMYTIIKT
IFKNANLTKTNRNFRFLQIFTAAAQAFAHGSNDAQKAMGIITMALISAGMQTGSTEPQTWVQISCAAAMGLGTAVGGWKI
IKTVGGNIMKIRPANGVAADLSSAFIIFGATHFHLPVSTTHVVSSSILGVGSSHRVKGVKWTTAQRMIITWVITLPISAL
IAAIIYFAVNFITHIF
>Mature_336_residues
MEPIIIFTFAIVFFALAFDFINGFHDTANAIATAVSTKALKPRHAILMAAVLNFIGAILFHGVAKTVTKDIVDPFTLQNG
QVVILAALISAITWNLITWYFGIPSSSSHTLIGSIAGAAIASGGFGILEYAGFTKIIVGLLISPLLAFIVGYIMYTIIKT
IFKNANLTKTNRNFRFLQIFTAAAQAFAHGSNDAQKAMGIITMALISAGMQTGSTEPQTWVQISCAAAMGLGTAVGGWKI
IKTVGGNIMKIRPANGVAADLSSAFIIFGATHFHLPVSTTHVVSSSILGVGSSHRVKGVKWTTAQRMIITWVITLPISAL
IAAIIYFAVNFITHIF

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=157, Percent_Identity=34.3949044585987, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI5803173, Length=154, Percent_Identity=33.1168831168831, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1789360, Length=219, Percent_Identity=31.5068493150685, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789907, Length=217, Percent_Identity=32.7188940092166, Blast_Score=115, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71981576, Length=184, Percent_Identity=35.3260869565217, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25146401, Length=162, Percent_Identity=35.8024691358025, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17539280, Length=157, Percent_Identity=31.8471337579618, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536725, Length=165, Percent_Identity=30.9090909090909, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32566716, Length=167, Percent_Identity=31.7365269461078, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=155, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356511, Length=180, Percent_Identity=30, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 35751; Mature: 35751

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPIIIFTFAIVFFALAFDFINGFHDTANAIATAVSTKALKPRHAILMAAVLNFIGAILF
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HGVAKTVTKDIVDPFTLQNGQVVILAALISAITWNLITWYFGIPSSSSHTLIGSIAGAAI
HHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHH
ASGGFGILEYAGFTKIIVGLLISPLLAFIVGYIMYTIIKTIFKNANLTKTNRNFRFLQIF
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEHHHHH
TAAAQAFAHGSNDAQKAMGIITMALISAGMQTGSTEPQTWVQISCAAAMGLGTAVGGWKI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHHHCCCCCCCCHHH
IKTVGGNIMKIRPANGVAADLSSAFIIFGATHFHLPVSTTHVVSSSILGVGSSHRVKGVK
HHHHCCCEEEEECCCCCHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCEE
WTTAQRMIITWVITLPISALIAAIIYFAVNFITHIF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEPIIIFTFAIVFFALAFDFINGFHDTANAIATAVSTKALKPRHAILMAAVLNFIGAILF
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HGVAKTVTKDIVDPFTLQNGQVVILAALISAITWNLITWYFGIPSSSSHTLIGSIAGAAI
HHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHH
ASGGFGILEYAGFTKIIVGLLISPLLAFIVGYIMYTIIKTIFKNANLTKTNRNFRFLQIF
HCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEHHHHH
TAAAQAFAHGSNDAQKAMGIITMALISAGMQTGSTEPQTWVQISCAAAMGLGTAVGGWKI
HHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHEEEEHHHHHCCCCCCCCHHH
IKTVGGNIMKIRPANGVAADLSSAFIIFGATHFHLPVSTTHVVSSSILGVGSSHRVKGVK
HHHHCCCEEEEECCCCCHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCCCCCEE
WTTAQRMIITWVITLPISALIAAIIYFAVNFITHIF
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]