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The map label for this gene is lmrB [H]

Identifier: 222150648

GI number: 222150648

Start: 425309

End: 426751

Strand: Direct

Name: lmrB [H]

Synonym: MCCL_0398

Alternate gene names: 222150648

Gene position: 425309-426751 (Clockwise)

Preceding gene: 222150646

Following gene: 222150649

Centisome position: 20.23

GC content: 39.36

Gene sequence:

>1443_bases
ATGAAAAAATCTACTATAATTATGACTACATTTTTAATTGGTGCATTCTTTACCTTTTTAAATGAAACTTTACTGAATAT
CGCACTAACGAAGATTATGACAGTTTTTCATGTGGATGCACCGACTGTGCAGTGGCTTGCTACTGGATTTATGCTTGTTA
TGGGAGTATTGATGCCATTATCTGCGACTATAATACAGTGGTTTACAACGCGACAATTATTTATAGGATTGATGTCGATC
TTTCTTATCGGGACATTGGTAGCAGGGTGTGCAGTGAATTTTCCTATGCTCTTAGCGGGCAGAATGATTCAGGCTGCAGG
TACAGGATTATTGATACCTGTTATTATGAATGCCATGCTGCTATTATTCCCGCCGTATGAACGTGGAAAGGTCATGGGGA
ATTTTGGTCTTGTAATGATGTTTGCGCCAGCAATTGGCCCGACACTTTCTGGCGTAATCGTTGACACATTAGGATGGAGA
TGGTTGTTTTTTGCTGTCGTACCATTCGTTGTATTTTCAATTGGATTTGCGTTTAAATATCTGGATAATGTCGGTGAAGT
GACAAAACCGAAAATCGATATATTCAGCGTTGTCTTATCAACAATCGGTGTTGCAGGTATTATATATGGCTTTAGCTCTG
TCGGGAATATAGAAGGTGGATTTAGCAATAAAGCAGTATTCTTGCCGATCGTTATTGGAGTCATTTCCTTGATCATCTTT
ATTTACCGTCAAAATCATTTGACATCACCACTACTTGATATGTCCGTATTTAAGTATAGCAACTATAGTAAAGGGATGTT
TATCTTTGTCGTGGTTGTTATGGCGATGTTTGCTTCTGAGATTGTGATGCCGATGTACTTGCAAGGGCCGATGGGATTTA
GTGCCAAAGTTGCAGGTATGATCCTGTTACCAGGTGCTTTATTAAATGGCGCGATGAGCCCGGTTATGGGCCGAATATTT
GATAAGATTGGTCCACGTAAGATGATCATTCCAGGTATGTTTGTTCTGACGCTCGTCATGATATTCTACTCGACGATTCA
CCCAGGTATACCATTATATATATTTATCATTGTTTATATGGTGCTCATGGTATCTATTTCGATGATTATGATGCCAAGTC
ATACGAATGCAATCAATCAGTTACCAAAACATTTGTATCCGCATGGAACAGCAATCGGTAACATGATACAACCAATTGCC
GGTGCTATGGGAATTTCAGTCTTTGTAAGTATAATGACACATGGCCAGCAAATTGCATTAGAAGGAATAAGTAACCCTAC
CCATTCACAGGCTCAAGCGGCACTGACATATGGTGTACATCACGCTTATTGGTTTGCGATAGCTCTTACACTCATAGGTT
TAATTACAGCCTTATTTATCACACGTTCCAAGGCACCTGAAGGCGAAACATTCGGACTACCTGAAGAACAAAATCAAATT
TAA

Upstream 100 bases:

>100_bases
TACTTCATAACAATAAGCATATAAAATAATGAACGTGAGGTATTGTTTTTATATATATTGTACTTTAAGATGTATAGACA
TCTATAATTTCGGAGGAAAT

Downstream 100 bases:

>100_bases
CGTTTATGACCTTTAATTTCGGGTATTAAAGACTTGTTGTGAAATAATCATAGCAAGTCTTTTATTATATTAAGGGAGAG
GTTGGATGTCTAAAGGTAAT

Product: drug-export protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MKKSTIIMTTFLIGAFFTFLNETLLNIALTKIMTVFHVDAPTVQWLATGFMLVMGVLMPLSATIIQWFTTRQLFIGLMSI
FLIGTLVAGCAVNFPMLLAGRMIQAAGTGLLIPVIMNAMLLLFPPYERGKVMGNFGLVMMFAPAIGPTLSGVIVDTLGWR
WLFFAVVPFVVFSIGFAFKYLDNVGEVTKPKIDIFSVVLSTIGVAGIIYGFSSVGNIEGGFSNKAVFLPIVIGVISLIIF
IYRQNHLTSPLLDMSVFKYSNYSKGMFIFVVVVMAMFASEIVMPMYLQGPMGFSAKVAGMILLPGALLNGAMSPVMGRIF
DKIGPRKMIIPGMFVLTLVMIFYSTIHPGIPLYIFIIVYMVLMVSISMIMMPSHTNAINQLPKHLYPHGTAIGNMIQPIA
GAMGISVFVSIMTHGQQIALEGISNPTHSQAQAALTYGVHHAYWFAIALTLIGLITALFITRSKAPEGETFGLPEEQNQI

Sequences:

>Translated_480_residues
MKKSTIIMTTFLIGAFFTFLNETLLNIALTKIMTVFHVDAPTVQWLATGFMLVMGVLMPLSATIIQWFTTRQLFIGLMSI
FLIGTLVAGCAVNFPMLLAGRMIQAAGTGLLIPVIMNAMLLLFPPYERGKVMGNFGLVMMFAPAIGPTLSGVIVDTLGWR
WLFFAVVPFVVFSIGFAFKYLDNVGEVTKPKIDIFSVVLSTIGVAGIIYGFSSVGNIEGGFSNKAVFLPIVIGVISLIIF
IYRQNHLTSPLLDMSVFKYSNYSKGMFIFVVVVMAMFASEIVMPMYLQGPMGFSAKVAGMILLPGALLNGAMSPVMGRIF
DKIGPRKMIIPGMFVLTLVMIFYSTIHPGIPLYIFIIVYMVLMVSISMIMMPSHTNAINQLPKHLYPHGTAIGNMIQPIA
GAMGISVFVSIMTHGQQIALEGISNPTHSQAQAALTYGVHHAYWFAIALTLIGLITALFITRSKAPEGETFGLPEEQNQI
>Mature_480_residues
MKKSTIIMTTFLIGAFFTFLNETLLNIALTKIMTVFHVDAPTVQWLATGFMLVMGVLMPLSATIIQWFTTRQLFIGLMSI
FLIGTLVAGCAVNFPMLLAGRMIQAAGTGLLIPVIMNAMLLLFPPYERGKVMGNFGLVMMFAPAIGPTLSGVIVDTLGWR
WLFFAVVPFVVFSIGFAFKYLDNVGEVTKPKIDIFSVVLSTIGVAGIIYGFSSVGNIEGGFSNKAVFLPIVIGVISLIIF
IYRQNHLTSPLLDMSVFKYSNYSKGMFIFVVVVMAMFASEIVMPMYLQGPMGFSAKVAGMILLPGALLNGAMSPVMGRIF
DKIGPRKMIIPGMFVLTLVMIFYSTIHPGIPLYIFIIVYMVLMVSISMIMMPSHTNAINQLPKHLYPHGTAIGNMIQPIA
GAMGISVFVSIMTHGQQIALEGISNPTHSQAQAALTYGVHHAYWFAIALTLIGLITALFITRSKAPEGETFGLPEEQNQI

Specific function: Proton-dependent transporter. May mediate the efflux of lincomycin [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=435, Percent_Identity=24.367816091954, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1788710, Length=426, Percent_Identity=23.2394366197183, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1790195, Length=397, Percent_Identity=22.4181360201511, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87081983, Length=377, Percent_Identity=24.4031830238727, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1788392, Length=444, Percent_Identity=20.9459459459459, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52378; Mature: 52378

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
7.3 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
7.3 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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>Mature Secondary Structure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PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274031; 9384377 [H]