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The map label for this gene is gloB [H]

Identifier: 222150634

GI number: 222150634

Start: 412467

End: 413780

Strand: Direct

Name: gloB [H]

Synonym: MCCL_0384

Alternate gene names: 222150634

Gene position: 412467-413780 (Clockwise)

Preceding gene: 222150633

Following gene: 222150636

Centisome position: 19.62

GC content: 41.25

Gene sequence:

>1314_bases
ATGTATTTTAAACAATTTTTTAATGAGCAACTTGCACAGTATTCTTATTTAGTAGGATGTCAAAAAACAGGAGAAGCTAT
TGTCATTGATCCGTTACGTGATATTACACCTTATATAGAGGCAGCAGAAAAAGAAGGACTACGCATTACGCATGTGACTG
AAACACATATTCATGCAGACTTTGCATCAGGTATAAGAGAAGTCATCAATATGGGAACGCAAAGCTATGTTTCTGGGCAT
GGCGGTCTTGACTGGACATATCAGGACATTGAAGCAGACATTATCCATGAAGGAGATGTCATTAAAGTAGGGAATGTGAA
GCTTGAAGTCATGCATACCCCTGGGCATACTCCTGAAAGTATTAGTTTCTTGCTCTATGATAATGGAATGGATGTGCCGA
TGAGCTTATTTACAGGAGACTTCATCTTTGTCGGTGATGTTGGACGTCCTGATTTATTAGAAGAAGCGGCTGGTATTAAA
GATACGACTGAAATAGGCGCAAGACAGATGTTCCAGTCACTGAAGAAGGTGAATGACCTTCCAGATTTCATTCAAATATG
GCCAGGTCATGGCGCAGGCAGTGCATGTGGTAAATCCTTAGGCGCTGTCCCGATGACGACACTCGGCTATGAACGTACGA
ATGGCTGGGCGTTCCAGATTGAAGAGGAGGATGCATTCATCAAAGCATTAACGAGCGATCAACCCGAACCTCCATACTAC
TTTGCTGAGATGAAACGTATGAATCGTGACGGTGTAGCGCTTATGCATCACAATATTGTAACACCTGTAGAGACGCTTAC
AGAACAGGCGATAGATCTCCGAGCGAAAGAAACCTACGCCAATAATATGTCAGAAGGCATCAATGTACCATACAATAAGA
AGTTTCTATCATTTGCAGGCTGGTACTTAGATTACAGCCGTCCATTACAGCTTATTGGAGAGTTGGACGAAGTTCAAGAA
GCAGCGCATGATCTAAAGATGATAGGCTTTGACGATGTTCATACTTATGTTAATGTCGTTGATGGAACTGCATATCAAAC
AATCGAACCAGAAGCATTTAAACAGCTGAATAAAGATGATATCAATATACTGGATGTGCGAACGAAAAAGGAATGGGATG
AAGGACATTTTGACGGCGCTGTACATGTTCATTATGGCAAACTGCAGCAAGCATCTATTCCATTTGCCAAGGATGAAGAA
ATTTATGTGCACTGTCAGTCAGGTGTTAGAAGCGCGATTGCAATGAGTATTCTAGAAAATGAAGGCTATACGAATTTAAT
CAATGTGAACGGTGGCTATGCTGCTGTAAAATAA

Upstream 100 bases:

>100_bases
AAAGAAGAGTTAATCGATGGCGTTGAGTTTGGCGGTGTTGCAACATATCTCGGTGATACAGAACAATCGAACTTAAACTT
ATTTATTTAGGAGGCATATG

Downstream 100 bases:

>100_bases
AAAAGATGACATGTCTGAAGCACACCCCTAAGGTTCAATCTTAGGGGTGTGCTTCATTTAATGGATATGACCTGTTGTAA
ATTTAATATCTTCCTGATAC

Product: hypothetical protein

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MYFKQFFNEQLAQYSYLVGCQKTGEAIVIDPLRDITPYIEAAEKEGLRITHVTETHIHADFASGIREVINMGTQSYVSGH
GGLDWTYQDIEADIIHEGDVIKVGNVKLEVMHTPGHTPESISFLLYDNGMDVPMSLFTGDFIFVGDVGRPDLLEEAAGIK
DTTEIGARQMFQSLKKVNDLPDFIQIWPGHGAGSACGKSLGAVPMTTLGYERTNGWAFQIEEEDAFIKALTSDQPEPPYY
FAEMKRMNRDGVALMHHNIVTPVETLTEQAIDLRAKETYANNMSEGINVPYNKKFLSFAGWYLDYSRPLQLIGELDEVQE
AAHDLKMIGFDDVHTYVNVVDGTAYQTIEPEAFKQLNKDDINILDVRTKKEWDEGHFDGAVHVHYGKLQQASIPFAKDEE
IYVHCQSGVRSAIAMSILENEGYTNLINVNGGYAAVK

Sequences:

>Translated_437_residues
MYFKQFFNEQLAQYSYLVGCQKTGEAIVIDPLRDITPYIEAAEKEGLRITHVTETHIHADFASGIREVINMGTQSYVSGH
GGLDWTYQDIEADIIHEGDVIKVGNVKLEVMHTPGHTPESISFLLYDNGMDVPMSLFTGDFIFVGDVGRPDLLEEAAGIK
DTTEIGARQMFQSLKKVNDLPDFIQIWPGHGAGSACGKSLGAVPMTTLGYERTNGWAFQIEEEDAFIKALTSDQPEPPYY
FAEMKRMNRDGVALMHHNIVTPVETLTEQAIDLRAKETYANNMSEGINVPYNKKFLSFAGWYLDYSRPLQLIGELDEVQE
AAHDLKMIGFDDVHTYVNVVDGTAYQTIEPEAFKQLNKDDINILDVRTKKEWDEGHFDGAVHVHYGKLQQASIPFAKDEE
IYVHCQSGVRSAIAMSILENEGYTNLINVNGGYAAVK
>Mature_437_residues
MYFKQFFNEQLAQYSYLVGCQKTGEAIVIDPLRDITPYIEAAEKEGLRITHVTETHIHADFASGIREVINMGTQSYVSGH
GGLDWTYQDIEADIIHEGDVIKVGNVKLEVMHTPGHTPESISFLLYDNGMDVPMSLFTGDFIFVGDVGRPDLLEEAAGIK
DTTEIGARQMFQSLKKVNDLPDFIQIWPGHGAGSACGKSLGAVPMTTLGYERTNGWAFQIEEEDAFIKALTSDQPEPPYY
FAEMKRMNRDGVALMHHNIVTPVETLTEQAIDLRAKETYANNMSEGINVPYNKKFLSFAGWYLDYSRPLQLIGELDEVQE
AAHDLKMIGFDDVHTYVNVVDGTAYQTIEPEAFKQLNKDDINILDVRTKKEWDEGHFDGAVHVHYGKLQQASIPFAKDEE
IYVHCQSGVRSAIAMSILENEGYTNLINVNGGYAAVK

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=195, Percent_Identity=31.2820512820513, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI116642887, Length=205, Percent_Identity=29.2682926829268, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI21703352, Length=207, Percent_Identity=29.4685990338164, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI14150041, Length=180, Percent_Identity=30, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI46361987, Length=180, Percent_Identity=30, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI94538322, Length=179, Percent_Identity=29.608938547486, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI94538320, Length=178, Percent_Identity=29.7752808988764, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1787158, Length=231, Percent_Identity=28.1385281385281, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538952, Length=194, Percent_Identity=28.8659793814433, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI221330176, Length=202, Percent_Identity=27.7227722772277, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 48908; Mature: 48908

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYFKQFFNEQLAQYSYLVGCQKTGEAIVIDPLRDITPYIEAAEKEGLRITHVTETHIHAD
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
FASGIREVINMGTQSYVSGHGGLDWTYQDIEADIIHEGDVIKVGNVKLEVMHTPGHTPES
HHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCEEEEECCCEEEECCEEEEEEECCCCCCCC
ISFLLYDNGMDVPMSLFTGDFIFVGDVGRPDLLEEAAGIKDTTEIGARQMFQSLKKVNDL
EEEEEEECCCCCCHHHHCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
PDFIQIWPGHGAGSACGKSLGAVPMTTLGYERTNGWAFQIEEEDAFIKALTSDQPEPPYY
CCHHEECCCCCCCHHHHHCCCCCCCHHCCEECCCCEEEEEECCCHHHHHHCCCCCCCCHH
FAEMKRMNRDGVALMHHNIVTPVETLTEQAIDLRAKETYANNMSEGINVPYNKKFLSFAG
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHH
WYLDYSRPLQLIGELDEVQEAAHDLKMIGFDDVHTYVNVVDGTAYQTIEPEAFKQLNKDD
HEECCCCHHHHHCCHHHHHHHHHHHEECCCHHHHHHHEEECCCEEECCCHHHHHHCCCCC
INILDVRTKKEWDEGHFDGAVHVHYGKLQQASIPFAKDEEIYVHCQSGVRSAIAMSILEN
CEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHC
EGYTNLINVNGGYAAVK
CCCEEEEECCCCEEECC
>Mature Secondary Structure
MYFKQFFNEQLAQYSYLVGCQKTGEAIVIDPLRDITPYIEAAEKEGLRITHVTETHIHAD
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
FASGIREVINMGTQSYVSGHGGLDWTYQDIEADIIHEGDVIKVGNVKLEVMHTPGHTPES
HHHHHHHHHHCCCHHHHCCCCCCCEEHHCCCEEEEECCCEEEECCEEEEEEECCCCCCCC
ISFLLYDNGMDVPMSLFTGDFIFVGDVGRPDLLEEAAGIKDTTEIGARQMFQSLKKVNDL
EEEEEEECCCCCCHHHHCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCC
PDFIQIWPGHGAGSACGKSLGAVPMTTLGYERTNGWAFQIEEEDAFIKALTSDQPEPPYY
CCHHEECCCCCCCHHHHHCCCCCCCHHCCEECCCCEEEEEECCCHHHHHHCCCCCCCCHH
FAEMKRMNRDGVALMHHNIVTPVETLTEQAIDLRAKETYANNMSEGINVPYNKKFLSFAG
HHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHH
WYLDYSRPLQLIGELDEVQEAAHDLKMIGFDDVHTYVNVVDGTAYQTIEPEAFKQLNKDD
HEECCCCHHHHHCCHHHHHHHHHHHEECCCHHHHHHHEEECCCEEECCCHHHHHHCCCCC
INILDVRTKKEWDEGHFDGAVHVHYGKLQQASIPFAKDEEIYVHCQSGVRSAIAMSILEN
CEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHC
EGYTNLINVNGGYAAVK
CCCEEEEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA