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The map label for this gene is gfo [H]

Identifier: 222150608

GI number: 222150608

Start: 374790

End: 375806

Strand: Direct

Name: gfo [H]

Synonym: MCCL_0358

Alternate gene names: 222150608

Gene position: 374790-375806 (Clockwise)

Preceding gene: 222150607

Following gene: 222150609

Centisome position: 17.83

GC content: 36.48

Gene sequence:

>1017_bases
ATGGCACAATTAAATGTAGGTATTATTGGAGTAGGTAGCATTGCTACACATAGACATATCCCTGAATATCAGCTTTTGGA
AGATGTAAATATTGTTGCATATTGTGATATTGTACAGGAGCGTGCTTCTGAATATGCAGAACAATTTGGTGGCAAAAGTT
ACGATAACTATAAAGCATTACTACAAGATGATTCTATTGATGCAGTTAGTATCTGTACACCAAACTATTTACATGCTGAG
ATTGCGATAGAAGCTTTAACAGCAGGAAAGCATGTACTGTGTGAAAAACCGATGGCAACAACGATTGAAGATGCAAAACG
TATGATTGAAGCAGCAACCAACTCAGGAAAACAATTAATGATTGCTCAAAATCAACGATTTACAGAAGCACATGAACTAG
CTCGTGAAAAACTTTCACAAGGTAAGCTAGGTAAAATTTATTCATTCAAAACAACCTTTGGCCATGCTGGTCCGGAGTCA
TGGAGTGTGGACGGTGCTAATAGCTGGTTCTTTGACAAAAGACGCGCTTTTATTGGTGCTATGGGGGATTTAGGTGTTCA
TAAAATTGACTTAATGCATTACTTATTGAATGAAACGTTCACAGAAGTTGGTGCGATGATCGAAACAAGTGAAAAAGAAG
GTACTGTTGATGATAATGCAGTCTTTATTCTGAAAACTAAAAGTGGTGCTATTGGAACAATGAGTGCTGCATGGGTATAC
AAGGGTGAAGAAGATAACAGTACAATTATTTATGGTGAAAAAGGCATATTAAAGCTTAACACAGATTCTGAATTTAACTA
TATCTTTATTGATGAAGAAGGCAATAAAGAAGCATATAAATTAGGCAAGATACAAACAAATGACGCACAAACGTCAACAG
GTACGATAGAGCGATTTATTGAAGCGATTCAAGGTGGTCAACCTGTACCAGTAAACGGGCAGGATGGATTAGCATCATTA
AATGTAATCGTAAAAGCTTTGCAATCAGCACAAGAGAAAAAAGTGGTGAAGATTTAA

Upstream 100 bases:

>100_bases
GTCATTAAAAATGCAGTGAATTGGGTGAAACCAACGCAATCAACATATCCGACGTATGGTAATCATCAACCGATAGAATC
GATACAGAAAGGTGAATAAT

Downstream 100 bases:

>100_bases
ATGGAACAATTAAAGATTGGTATCGTTGGAGCAGGAGGAATCGCAACAGGTAGACACATCCCTACATTCCAGCAGTTCCA
GGAAGTTAGTGTAAGTCATA

Product: NADH-dependent dyhydrogenase family protein

Products: NA

Alternate protein names: GFOR [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MAQLNVGIIGVGSIATHRHIPEYQLLEDVNIVAYCDIVQERASEYAEQFGGKSYDNYKALLQDDSIDAVSICTPNYLHAE
IAIEALTAGKHVLCEKPMATTIEDAKRMIEAATNSGKQLMIAQNQRFTEAHELAREKLSQGKLGKIYSFKTTFGHAGPES
WSVDGANSWFFDKRRAFIGAMGDLGVHKIDLMHYLLNETFTEVGAMIETSEKEGTVDDNAVFILKTKSGAIGTMSAAWVY
KGEEDNSTIIYGEKGILKLNTDSEFNYIFIDEEGNKEAYKLGKIQTNDAQTSTGTIERFIEAIQGGQPVPVNGQDGLASL
NVIVKALQSAQEKKVVKI

Sequences:

>Translated_338_residues
MAQLNVGIIGVGSIATHRHIPEYQLLEDVNIVAYCDIVQERASEYAEQFGGKSYDNYKALLQDDSIDAVSICTPNYLHAE
IAIEALTAGKHVLCEKPMATTIEDAKRMIEAATNSGKQLMIAQNQRFTEAHELAREKLSQGKLGKIYSFKTTFGHAGPES
WSVDGANSWFFDKRRAFIGAMGDLGVHKIDLMHYLLNETFTEVGAMIETSEKEGTVDDNAVFILKTKSGAIGTMSAAWVY
KGEEDNSTIIYGEKGILKLNTDSEFNYIFIDEEGNKEAYKLGKIQTNDAQTSTGTIERFIEAIQGGQPVPVNGQDGLASL
NVIVKALQSAQEKKVVKI
>Mature_337_residues
AQLNVGIIGVGSIATHRHIPEYQLLEDVNIVAYCDIVQERASEYAEQFGGKSYDNYKALLQDDSIDAVSICTPNYLHAEI
AIEALTAGKHVLCEKPMATTIEDAKRMIEAATNSGKQLMIAQNQRFTEAHELAREKLSQGKLGKIYSFKTTFGHAGPESW
SVDGANSWFFDKRRAFIGAMGDLGVHKIDLMHYLLNETFTEVGAMIETSEKEGTVDDNAVFILKTKSGAIGTMSAAWVYK
GEEDNSTIIYGEKGILKLNTDSEFNYIFIDEEGNKEAYKLGKIQTNDAQTSTGTIERFIEAIQGGQPVPVNGQDGLASLN
VIVKALQSAQEKKVVKI

Specific function: Unknown

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=270, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787574, Length=363, Percent_Identity=28.3746556473829, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI87082405, Length=311, Percent_Identity=26.0450160771704, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789848, Length=188, Percent_Identity=29.7872340425532, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI87081947, Length=102, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323975, Length=267, Percent_Identity=28.0898876404494, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24581117, Length=259, Percent_Identity=29.3436293436293, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008354
- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.99.28 [H]

Molecular weight: Translated: 37144; Mature: 37013

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQLNVGIIGVGSIATHRHIPEYQLLEDVNIVAYCDIVQERASEYAEQFGGKSYDNYKAL
CCEEEEEEEEECCHHHHCCCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHH
LQDDSIDAVSICTPNYLHAEIAIEALTAGKHVLCEKPMATTIEDAKRMIEAATNSGKQLM
EECCCCCEEEECCCCCEEHHHEEEEHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEE
IAQNQRFTEAHELAREKLSQGKLGKIYSFKTTFGHAGPESWSVDGANSWFFDKRRAFIGA
EECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
MGDLGVHKIDLMHYLLNETFTEVGAMIETSEKEGTVDDNAVFILKTKSGAIGTMSAAWVY
HCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEE
KGEEDNSTIIYGEKGILKLNTDSEFNYIFIDEEGNKEAYKLGKIQTNDAQTSTGTIERFI
ECCCCCCEEEECCCCEEEECCCCCEEEEEEECCCCCCCEEECCEECCCCCCCHHHHHHHH
EAIQGGQPVPVNGQDGLASLNVIVKALQSAQEKKVVKI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AQLNVGIIGVGSIATHRHIPEYQLLEDVNIVAYCDIVQERASEYAEQFGGKSYDNYKAL
CEEEEEEEEECCHHHHCCCCCHHHHHCCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHH
LQDDSIDAVSICTPNYLHAEIAIEALTAGKHVLCEKPMATTIEDAKRMIEAATNSGKQLM
EECCCCCEEEECCCCCEEHHHEEEEHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEE
IAQNQRFTEAHELAREKLSQGKLGKIYSFKTTFGHAGPESWSVDGANSWFFDKRRAFIGA
EECCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHH
MGDLGVHKIDLMHYLLNETFTEVGAMIETSEKEGTVDDNAVFILKTKSGAIGTMSAAWVY
HCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCCEEEEEE
KGEEDNSTIIYGEKGILKLNTDSEFNYIFIDEEGNKEAYKLGKIQTNDAQTSTGTIERFI
ECCCCCCEEEECCCCEEEECCCCCEEEEEEECCCCCCCEEECCEECCCCCCCHHHHHHHH
EAIQGGQPVPVNGQDGLASLNVIVKALQSAQEKKVVKI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1537789; 8472911; 8994968 [H]