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The map label for this gene is pbuE [H]

Identifier: 222150523

GI number: 222150523

Start: 276761

End: 277903

Strand: Direct

Name: pbuE [H]

Synonym: MCCL_0273

Alternate gene names: 222150523

Gene position: 276761-277903 (Clockwise)

Preceding gene: 222150522

Following gene: 222150526

Centisome position: 13.16

GC content: 38.23

Gene sequence:

>1143_bases
ATGAAGGTTAATTTGTTTTTACTTGCGATGATGGCATTCTTAGCAGGGTTAAATGAGCTTGTGCTAGCAGGTATTCTCCC
GTTCGTGATGGATTACTTTAATATTAATACGCAAGAAGCGGGTTATTTAATAACCGTCTTCGCATTAATATTTGGTCTTG
CAGGTCCTGTTCTGATGAGTGTAACGATGAATGTAAATCGTAAGACGCTATTATTAGTTTTCTTATCGATATTTATCGTT
GGTAATTTAATGAGTGTGTTTGCGCAATTCTACTGGATGATGATGCTATCGCGCATCGTACTTGCTGCAAGTGTATCGAT
GTTGATAGGCTTAGCATTATCGTTATCTGTTAAAGTAGTACCTGAGTATATGCAAGCGCGTGCGATTGGCATTATTATGA
TGAGCATCAGTGGTTCTTTAGTACTGGGAACGCCCATTGGTATTTATATTGCAGAACTGACAGACTGGAAAGTGATATTT
ATATTGATCAGTGTTTTATCGGCAATACTAATGCTGTTACTGATGAAGTATTTCCCGAAGAATATTCAAGTTGAACGTGT
CTCTATGGCACGTGACTGGAAGTTTATATTGAAGAAAGAAATTTTATCTGTCCATTTGTTCAGTTTCTTATTCTTTGTAG
GGCAGGGCATGTTTTTCGTTTATTTAACGCCGTACTTAATGGATAGAGGTCATAGCATCGCTGTTGTAGGCTTAATTTAT
CTCGTGTTTGGTCTTGCGGGGATTCTCGGCAGTGGTATTGGTGGCTATATGACAGATAAGTTTGGGAGTACGAACTTACT
TGTAGTGCAGTCGTTTTTATATGTATTTTTAATGCTGATGGTGAATTTTACAGCATTTTCTGCCGTGCTGGTATGTATTA
ATATCGCGATATGGGCGATGACGGGGTGGATGGTAAATGCTCCGACGCAAACATATTTAATACAAGCAGCACCAAAGTTC
TCGAGTATTAATCAAACTGTAAGTACATCATCAACGCAGTTCGGTATTTCTCTAGGTGCACTGTTAGGTGGCATCTGTAT
TACCCAGACGAATTCATATCCTGCGCTCATTTATTATGGATGCTGTGTTCTTGTAATTGCATTTTTAGTAACGCTATATA
TACGCAGACTTAATACGAAATAA

Upstream 100 bases:

>100_bases
CTTTATAATATTTCTAAAGGTGGCTTTGTGCCGGTTTTCGTTTATACGATGATTACAGCGCCTTCCGATATGGAAGGCGC
TGTTTATTTTGGAGGTTATA

Downstream 100 bases:

>100_bases
GGAGTGGCAGGGGCCACTCCTTATTTTGTATGTATAGATGAATGTTTAAGGGATTTATGTGTTACCGCATGATGAATGAT
GCTGACAACTTTAGAAATTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKVNLFLLAMMAFLAGLNELVLAGILPFVMDYFNINTQEAGYLITVFALIFGLAGPVLMSVTMNVNRKTLLLVFLSIFIV
GNLMSVFAQFYWMMMLSRIVLAASVSMLIGLALSLSVKVVPEYMQARAIGIIMMSISGSLVLGTPIGIYIAELTDWKVIF
ILISVLSAILMLLLMKYFPKNIQVERVSMARDWKFILKKEILSVHLFSFLFFVGQGMFFVYLTPYLMDRGHSIAVVGLIY
LVFGLAGILGSGIGGYMTDKFGSTNLLVVQSFLYVFLMLMVNFTAFSAVLVCINIAIWAMTGWMVNAPTQTYLIQAAPKF
SSINQTVSTSSTQFGISLGALLGGICITQTNSYPALIYYGCCVLVIAFLVTLYIRRLNTK

Sequences:

>Translated_380_residues
MKVNLFLLAMMAFLAGLNELVLAGILPFVMDYFNINTQEAGYLITVFALIFGLAGPVLMSVTMNVNRKTLLLVFLSIFIV
GNLMSVFAQFYWMMMLSRIVLAASVSMLIGLALSLSVKVVPEYMQARAIGIIMMSISGSLVLGTPIGIYIAELTDWKVIF
ILISVLSAILMLLLMKYFPKNIQVERVSMARDWKFILKKEILSVHLFSFLFFVGQGMFFVYLTPYLMDRGHSIAVVGLIY
LVFGLAGILGSGIGGYMTDKFGSTNLLVVQSFLYVFLMLMVNFTAFSAVLVCINIAIWAMTGWMVNAPTQTYLIQAAPKF
SSINQTVSTSSTQFGISLGALLGGICITQTNSYPALIYYGCCVLVIAFLVTLYIRRLNTK
>Mature_380_residues
MKVNLFLLAMMAFLAGLNELVLAGILPFVMDYFNINTQEAGYLITVFALIFGLAGPVLMSVTMNVNRKTLLLVFLSIFIV
GNLMSVFAQFYWMMMLSRIVLAASVSMLIGLALSLSVKVVPEYMQARAIGIIMMSISGSLVLGTPIGIYIAELTDWKVIF
ILISVLSAILMLLLMKYFPKNIQVERVSMARDWKFILKKEILSVHLFSFLFFVGQGMFFVYLTPYLMDRGHSIAVVGLIY
LVFGLAGILGSGIGGYMTDKFGSTNLLVVQSFLYVFLMLMVNFTAFSAVLVCINIAIWAMTGWMVNAPTQTYLIQAAPKF
SSINQTVSTSSTQFGISLGALLGGICITQTNSYPALIYYGCCVLVIAFLVTLYIRRLNTK

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine and 6-mercaptopurine. Therefore plays a role in maintaining t

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787947, Length=336, Percent_Identity=25.5952380952381, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693198, Length=351, Percent_Identity=21.0826210826211, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786595, Length=358, Percent_Identity=24.5810055865922, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787808, Length=222, Percent_Identity=22.0720720720721, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42003; Mature: 42003

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
6.3 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
6.3 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVNLFLLAMMAFLAGLNELVLAGILPFVMDYFNINTQEAGYLITVFALIFGLAGPVLMS
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VTMNVNRKTLLLVFLSIFIVGNLMSVFAQFYWMMMLSRIVLAASVSMLIGLALSLSVKVV
HEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PEYMQARAIGIIMMSISGSLVLGTPIGIYIAELTDWKVIFILISVLSAILMLLLMKYFPK
HHHHHHHHHHEEEEECCCCEEECCCHHEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
NIQVERVSMARDWKFILKKEILSVHLFSFLFFVGQGMFFVYLTPYLMDRGHSIAVVGLIY
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHCCCCCHHHHHHHH
LVFGLAGILGSGIGGYMTDKFGSTNLLVVQSFLYVFLMLMVNFTAFSAVLVCINIAIWAM
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGWMVNAPTQTYLIQAAPKFSSINQTVSTSSTQFGISLGALLGGICITQTNSYPALIYYG
HCCEEECCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCHHEEECCCCCHHHHHH
CCVLVIAFLVTLYIRRLNTK
HHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVNLFLLAMMAFLAGLNELVLAGILPFVMDYFNINTQEAGYLITVFALIFGLAGPVLMS
CCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VTMNVNRKTLLLVFLSIFIVGNLMSVFAQFYWMMMLSRIVLAASVSMLIGLALSLSVKVV
HEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PEYMQARAIGIIMMSISGSLVLGTPIGIYIAELTDWKVIFILISVLSAILMLLLMKYFPK
HHHHHHHHHHEEEEECCCCEEECCCHHEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHCCC
NIQVERVSMARDWKFILKKEILSVHLFSFLFFVGQGMFFVYLTPYLMDRGHSIAVVGLIY
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEHHHHHHHCCCCCHHHHHHHH
LVFGLAGILGSGIGGYMTDKFGSTNLLVVQSFLYVFLMLMVNFTAFSAVLVCINIAIWAM
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGWMVNAPTQTYLIQAAPKFSSINQTVSTSSTQFGISLGALLGGICITQTNSYPALIYYG
HCCEEECCCCEEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHCCHHEEECCCCCHHHHHH
CCVLVIAFLVTLYIRRLNTK
HHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA