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The map label for this gene is truA [H]

Identifier: 222150476

GI number: 222150476

Start: 220267

End: 221088

Strand: Direct

Name: truA [H]

Synonym: MCCL_0226

Alternate gene names: 222150476

Gene position: 220267-221088 (Clockwise)

Preceding gene: 222150475

Following gene: 222150477

Centisome position: 10.48

GC content: 41.12

Gene sequence:

>822_bases
ATGGAACGAATATTATTACAACTACAATATAATGGTGTGAACTTTCAAGGATTTCAGATACAAAACGAAGGACGCACCGT
ACAATATGAGTTAGAACGCATATTAAAACGCATGCACAAACAATTTGTCCGAATACACCCGTCATCACGTACGGACAAAG
GTGTGCACGCACGAATGCAATACGTTCACTTTGATACACCGCTCTCAATCCCGGAATCAAAGTGGCAGCATGCCTTTAAT
TCCGCATTACCTGACGATATATATATTACACAAGTAACGAAGATCAGTGAACATTTCCATTCACGATACGACTGTATCGG
CAAAGCATATCGCTATAAAGTATACGTTGCAGAACATCGCAATGTCATGCATAGCGGACAGATAACACATATTAAGACAA
AACTAGACGTTAAGAGAATGAACGACGCAGCACAACACTTTCTTGGCACGCATGACTTTACATCATTCTGTTCAGCAAAG
ACAGAAATAGAGAACAAAGAACGCACACTCTATCGTTTTGAAGTCACTGAGACAGCAGAAGGCTATGATTTCTATGTTAT
CGGAAGCGGCTTCCTCTACAATATGGTACGTATCATGGTGCAATATTTAATCGACGTCGGCACAGGACAAAAAGATGGCG
ACAGTATCCCTGCAATCATTCAGGCGCAAGATCGAACAAAAGCAGGCAAGACCGCCCCGGCAGAAGGACTCTATCTGGAG
AAAATCTATCTCGACACTGAAACATTAAAGCAAGATATACCACAAGGCACAGCCATTATCTTTAAAAATCAGAAACATAA
GATGAACGGCGAACAACTTTAA

Upstream 100 bases:

>100_bases
ATATCGTAAGCTCGACTGGCATACATATGACACGTTGTCACTCTTAACACTTATACCGATAACACTACTCATCCTCTATT
TAAAAAACTAGGAGCATATT

Downstream 100 bases:

>100_bases
CGAAAAATGAAAAATAAACACATAAATTATAGGAATACCATTGACAAACACCCTCTAAATGTTATAAGATAAACAACGGT
ATTGTTTAATATCAACCACG

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MERILLQLQYNGVNFQGFQIQNEGRTVQYELERILKRMHKQFVRIHPSSRTDKGVHARMQYVHFDTPLSIPESKWQHAFN
SALPDDIYITQVTKISEHFHSRYDCIGKAYRYKVYVAEHRNVMHSGQITHIKTKLDVKRMNDAAQHFLGTHDFTSFCSAK
TEIENKERTLYRFEVTETAEGYDFYVIGSGFLYNMVRIMVQYLIDVGTGQKDGDSIPAIIQAQDRTKAGKTAPAEGLYLE
KIYLDTETLKQDIPQGTAIIFKNQKHKMNGEQL

Sequences:

>Translated_273_residues
MERILLQLQYNGVNFQGFQIQNEGRTVQYELERILKRMHKQFVRIHPSSRTDKGVHARMQYVHFDTPLSIPESKWQHAFN
SALPDDIYITQVTKISEHFHSRYDCIGKAYRYKVYVAEHRNVMHSGQITHIKTKLDVKRMNDAAQHFLGTHDFTSFCSAK
TEIENKERTLYRFEVTETAEGYDFYVIGSGFLYNMVRIMVQYLIDVGTGQKDGDSIPAIIQAQDRTKAGKTAPAEGLYLE
KIYLDTETLKQDIPQGTAIIFKNQKHKMNGEQL
>Mature_273_residues
MERILLQLQYNGVNFQGFQIQNEGRTVQYELERILKRMHKQFVRIHPSSRTDKGVHARMQYVHFDTPLSIPESKWQHAFN
SALPDDIYITQVTKISEHFHSRYDCIGKAYRYKVYVAEHRNVMHSGQITHIKTKLDVKRMNDAAQHFLGTHDFTSFCSAK
TEIENKERTLYRFEVTETAEGYDFYVIGSGFLYNMVRIMVQYLIDVGTGQKDGDSIPAIIQAQDRTKAGKTAPAEGLYLE
KIYLDTETLKQDIPQGTAIIFKNQKHKMNGEQL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=271, Percent_Identity=30.6273062730627, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI199559785, Length=255, Percent_Identity=26.6666666666667, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788657, Length=254, Percent_Identity=34.251968503937, Blast_Score=154, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321112, Length=231, Percent_Identity=28.1385281385281, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 31718; Mature: 31718

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERILLQLQYNGVNFQGFQIQNEGRTVQYELERILKRMHKQFVRIHPSSRTDKGVHARMQ
CCEEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEE
YVHFDTPLSIPESKWQHAFNSALPDDIYITQVTKISEHFHSRYDCIGKAYRYKVYVAEHR
EEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEEEECCC
NVMHSGQITHIKTKLDVKRMNDAAQHFLGTHDFTSFCSAKTEIENKERTLYRFEVTETAE
CCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCC
GYDFYVIGSGFLYNMVRIMVQYLIDVGTGQKDGDSIPAIIQAQDRTKAGKTAPAEGLYLE
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEE
KIYLDTETLKQDIPQGTAIIFKNQKHKMNGEQL
EEEECHHHHHHHCCCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure
MERILLQLQYNGVNFQGFQIQNEGRTVQYELERILKRMHKQFVRIHPSSRTDKGVHARMQ
CCEEEEEEEECCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEE
YVHFDTPLSIPESKWQHAFNSALPDDIYITQVTKISEHFHSRYDCIGKAYRYKVYVAEHR
EEEECCCCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHCCEEEEEEEEECCC
NVMHSGQITHIKTKLDVKRMNDAAQHFLGTHDFTSFCSAKTEIENKERTLYRFEVTETAE
CCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCC
GYDFYVIGSGFLYNMVRIMVQYLIDVGTGQKDGDSIPAIIQAQDRTKAGKTAPAEGLYLE
CEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEE
KIYLDTETLKQDIPQGTAIIFKNQKHKMNGEQL
EEEECHHHHHHHCCCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA