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The map label for this gene is mtnE [H]

Identifier: 222150430

GI number: 222150430

Start: 188150

End: 189313

Strand: Direct

Name: mtnE [H]

Synonym: MCCL_0180

Alternate gene names: 222150430

Gene position: 188150-189313 (Clockwise)

Preceding gene: 222150429

Following gene: 222150433

Centisome position: 8.95

GC content: 38.4

Gene sequence:

>1164_bases
ATGTCTGTGAGTTCTAAATTATTAAATTCAATACCTGAAAGTTATTTTGGTAAGACGATGGGCAGGAAGCTTGAAGTCGG
GGATAAACCATTGATTAATACAGCAGTCGGTATCCCTGACCAACCAGTGGATCGTAAGGTGTTAAACGCATTACAAGATG
CCATTGTTGACCCTGATAACCATAGATATGGTGTATTCCGTGGCAAGGAGACATTGAAGAAAGGGATCCAGAAGTTTTAT
AAAGATATCTATGATGTTTCACTGGAGGATGATGAAGTTTGTATTCTTTATGGTACAAAAAATGGTCTTGTTCATATCCC
TGCATGTGTCATTGAACCTGGCGAGGGAGTGTTACTCCCTAATCCGGGATATACCGATTACCTTGCTGGTGTTAAGCTTG
CCAGAGGCTTACATTATGACTTACCATTACACCCTGAAGATCATTACTTACCTGACTTTGATAATATAGATAAAGCGCAG
CTTAATAATACAAAGCTGATTTATCTTAACTATCCATCTAATCCAACTGGAGCAGTAGCGACCCAAGCATTTTTTGATGA
TACAATTATGCGTTTTAAAGATACCGAAACAAAGATTGTCCATGACTTTGCCTATGCAGCTTTTGGATTTAATGGTAAGA
ATCCAAGCATTCTTGCTTCACCTCATGGGAAAGAAGTCGCTGTAGAAATATATTCGTTATCTAAAGGGTTTAATATGTCG
GGTGTACGTGTCGGGTTTGCTGTAGGGAATAAAGAGATTGTGAGTGCTCTGAACTATTTTCAGGATCATACTCAAGTTGG
TATGTGGGGCGTTGTACAAGATGCGGCGATAGCAGCGCTTGAGCTGGGAGAGCCATATCTAGAATGTCAAAACAATATAT
TTAAAGCACGACGCGATAAAGTGACAGCATATTTAAAGGAATATGAAATTCCGTTTGAGCCTATGAATGGTGGTATATTT
TTATGGATAAAAGTGCCGGACGGTTTTGATGGGGAATCATATACAGACCATTTATTGAAGCAAGAATCAATTTTAGTAAC
TCCAGGAATCCCTTTTGGGTCACGTGGTAAGAATTACATTAGAATCTCCCTTGCTATTGATGATGTGTTGATCAATGAAC
TGCTCGAGCGCTTAAAAAATACAAAGAAGTTATATATAAAATAA

Upstream 100 bases:

>100_bases
GTACTGTGCTAAATCCAGCAAGCATATGCTTGGTAGATAAGTGCCATATCGGCGTCATTGCTACTGAAGCAATGACGCTT
TTCTATTTTTAAAGGAGCGA

Downstream 100 bases:

>100_bases
GAACTTACAAAGCATTACTTTGTAAGTTCTTATTTTATAAGTATAGCGCACAAAGTGCGATAATCATTAGCGCCGTCATG
ATAGAGAAGAGATCGAGAAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSVSSKLLNSIPESYFGKTMGRKLEVGDKPLINTAVGIPDQPVDRKVLNALQDAIVDPDNHRYGVFRGKETLKKGIQKFY
KDIYDVSLEDDEVCILYGTKNGLVHIPACVIEPGEGVLLPNPGYTDYLAGVKLARGLHYDLPLHPEDHYLPDFDNIDKAQ
LNNTKLIYLNYPSNPTGAVATQAFFDDTIMRFKDTETKIVHDFAYAAFGFNGKNPSILASPHGKEVAVEIYSLSKGFNMS
GVRVGFAVGNKEIVSALNYFQDHTQVGMWGVVQDAAIAALELGEPYLECQNNIFKARRDKVTAYLKEYEIPFEPMNGGIF
LWIKVPDGFDGESYTDHLLKQESILVTPGIPFGSRGKNYIRISLAIDDVLINELLERLKNTKKLYIK

Sequences:

>Translated_387_residues
MSVSSKLLNSIPESYFGKTMGRKLEVGDKPLINTAVGIPDQPVDRKVLNALQDAIVDPDNHRYGVFRGKETLKKGIQKFY
KDIYDVSLEDDEVCILYGTKNGLVHIPACVIEPGEGVLLPNPGYTDYLAGVKLARGLHYDLPLHPEDHYLPDFDNIDKAQ
LNNTKLIYLNYPSNPTGAVATQAFFDDTIMRFKDTETKIVHDFAYAAFGFNGKNPSILASPHGKEVAVEIYSLSKGFNMS
GVRVGFAVGNKEIVSALNYFQDHTQVGMWGVVQDAAIAALELGEPYLECQNNIFKARRDKVTAYLKEYEIPFEPMNGGIF
LWIKVPDGFDGESYTDHLLKQESILVTPGIPFGSRGKNYIRISLAIDDVLINELLERLKNTKKLYIK
>Mature_386_residues
SVSSKLLNSIPESYFGKTMGRKLEVGDKPLINTAVGIPDQPVDRKVLNALQDAIVDPDNHRYGVFRGKETLKKGIQKFYK
DIYDVSLEDDEVCILYGTKNGLVHIPACVIEPGEGVLLPNPGYTDYLAGVKLARGLHYDLPLHPEDHYLPDFDNIDKAQL
NNTKLIYLNYPSNPTGAVATQAFFDDTIMRFKDTETKIVHDFAYAAFGFNGKNPSILASPHGKEVAVEIYSLSKGFNMSG
VRVGFAVGNKEIVSALNYFQDHTQVGMWGVVQDAAIAALELGEPYLECQNNIFKARRDKVTAYLKEYEIPFEPMNGGIFL
WIKVPDGFDGESYTDHLLKQESILVTPGIPFGSRGKNYIRISLAIDDVLINELLERLKNTKKLYIK

Specific function: Catalyzes the formation of methionine from 2-keto-4- methylthiobutyrate (KMTB) (Probable) [H]

COG id: COG0436

COG function: function code E; Aspartate/tyrosine/aromatic aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. MtnE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788722, Length=357, Percent_Identity=31.3725490196078, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI1786816, Length=357, Percent_Identity=22.96918767507, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994472, Length=392, Percent_Identity=22.7040816326531, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994476, Length=392, Percent_Identity=22.7040816326531, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17567369, Length=289, Percent_Identity=24.5674740484429, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322401, Length=389, Percent_Identity=21.5938303341902, Blast_Score=79, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: 2.6.1.- [C]

Molecular weight: Translated: 43219; Mature: 43088

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS00105 AA_TRANSFER_CLASS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSSKLLNSIPESYFGKTMGRKLEVGDKPLINTAVGIPDQPVDRKVLNALQDAIVDPDN
CCHHHHHHHHCCHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
HRYGVFRGKETLKKGIQKFYKDIYDVSLEDDEVCILYGTKNGLVHIPACVIEPGEGVLLP
CEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECEEEEECCCCEEEC
NPGYTDYLAGVKLARGLHYDLPLHPEDHYLPDFDNIDKAQLNNTKLIYLNYPSNPTGAVA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCCCCHHH
TQAFFDDTIMRFKDTETKIVHDFAYAAFGFNGKNPSILASPHGKEVAVEIYSLSKGFNMS
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEEECCCCCCC
GVRVGFAVGNKEIVSALNYFQDHTQVGMWGVVQDAAIAALELGEPYLECQNNIFKARRDK
CEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VTAYLKEYEIPFEPMNGGIFLWIKVPDGFDGESYTDHLLKQESILVTPGIPFGSRGKNYI
HHHHHHHCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCEE
RISLAIDDVLINELLERLKNTKKLYIK
EEEEEHHHHHHHHHHHHHCCCCEEECC
>Mature Secondary Structure 
SVSSKLLNSIPESYFGKTMGRKLEVGDKPLINTAVGIPDQPVDRKVLNALQDAIVDPDN
CHHHHHHHHCCHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
HRYGVFRGKETLKKGIQKFYKDIYDVSLEDDEVCILYGTKNGLVHIPACVIEPGEGVLLP
CEEEEEECHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEECEEEEECCCCEEEC
NPGYTDYLAGVKLARGLHYDLPLHPEDHYLPDFDNIDKAQLNNTKLIYLNYPSNPTGAVA
CCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCEEEEEEECCCCCCCHHH
TQAFFDDTIMRFKDTETKIVHDFAYAAFGFNGKNPSILASPHGKEVAVEIYSLSKGFNMS
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEECCCCCEEEEEEEEECCCCCCC
GVRVGFAVGNKEIVSALNYFQDHTQVGMWGVVQDAAIAALELGEPYLECQNNIFKARRDK
CEEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
VTAYLKEYEIPFEPMNGGIFLWIKVPDGFDGESYTDHLLKQESILVTPGIPFGSRGKNYI
HHHHHHHCCCCCEECCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEECCCCCCCCCCCEE
RISLAIDDVLINELLERLKNTKKLYIK
EEEEEHHHHHHHHHHHHHCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 12022921 [H]