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The map label for this gene is lrgB [H]

Identifier: 222150362

GI number: 222150362

Start: 117575

End: 118282

Strand: Direct

Name: lrgB [H]

Synonym: MCCL_0112

Alternate gene names: 222150362

Gene position: 117575-118282 (Clockwise)

Preceding gene: 222150361

Following gene: 222150363

Centisome position: 5.59

GC content: 36.72

Gene sequence:

>708_bases
ATGACATGGCTTGATCATTTAGGTATTAACTCTGTATACTTCGGAATATTATTAACGATATTACCATTTATGTTAGGTCA
ATATTTATTTAAAAAAACAAATGGATTTTTCTTATTTGCACCATTATTTGTCGGAATGGTATTTGGTATTGCATTCTTAT
CTATAACTGGTATCAGCTATGACACTTATAGTAAAGGTGGAAGTATTATTAGTTTCTTCTTAGAACCTGCAACGATCTGC
TTTGCAATTCCTCTTTATAAAAAGCGTGACGTATTACAAAAGTATTGGTTACATATCATCGGGGGTCTGTCACTTGGTAC
AGCAGCTGCGATGTTAGGTATATATGGAGTAGCAAAATTATTTGGATTCGGTACACAGATTATTGCATCAATGTTACCTC
AAGCAGCGACAACTGCTATTGCTTTACCGGTATCTGCTGGTATTGGTGGCGTACCAGAACTGACGTCATTGGCAGTTATT
CTAAATGCAGTTATTATTTATGCCCTAGGAAATAAAATGCTACGCTTCTTCAAAATCTCTAATCCAATTGCACGTGGGCT
TGCGCTGGGTACAAGTGGGCATGCACTAGGTGTTTCAGCTGCAACTGAACTTGGAGAAACTGAAACTTCTATGGCGAGTA
TTGCACTTGTACTTGTAGGTGTTGTAGTAGTTGTTATTGTACCGATATTAACGGGTATATTACTTTAA

Upstream 100 bases:

>100_bases
GGGTTCTTCTCTCAAGTGATTGTAAAGTTGACAGACTCAAAGGTAAAAGAACAAAAAGCAAAAGAAACGAAACATCTAAA
AGAAATAGAGGTGCGCTAAT

Downstream 100 bases:

>100_bases
TCTAGGATATAAAATAAAAAATATATCACCAATTCACATACCGAGACAAATATGTCTCGGTTTTTTTAGGTTGTAATTCT
TATGCAAAATTAAAATAAGT

Product: antiholin-like protein LrgB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MTWLDHLGINSVYFGILLTILPFMLGQYLFKKTNGFFLFAPLFVGMVFGIAFLSITGISYDTYSKGGSIISFFLEPATIC
FAIPLYKKRDVLQKYWLHIIGGLSLGTAAAMLGIYGVAKLFGFGTQIIASMLPQAATTAIALPVSAGIGGVPELTSLAVI
LNAVIIYALGNKMLRFFKISNPIARGLALGTSGHALGVSAATELGETETSMASIALVLVGVVVVVIVPILTGILL

Sequences:

>Translated_235_residues
MTWLDHLGINSVYFGILLTILPFMLGQYLFKKTNGFFLFAPLFVGMVFGIAFLSITGISYDTYSKGGSIISFFLEPATIC
FAIPLYKKRDVLQKYWLHIIGGLSLGTAAAMLGIYGVAKLFGFGTQIIASMLPQAATTAIALPVSAGIGGVPELTSLAVI
LNAVIIYALGNKMLRFFKISNPIARGLALGTSGHALGVSAATELGETETSMASIALVLVGVVVVVIVPILTGILL
>Mature_234_residues
TWLDHLGINSVYFGILLTILPFMLGQYLFKKTNGFFLFAPLFVGMVFGIAFLSITGISYDTYSKGGSIISFFLEPATICF
AIPLYKKRDVLQKYWLHIIGGLSLGTAAAMLGIYGVAKLFGFGTQIIASMLPQAATTAIALPVSAGIGGVPELTSLAVIL
NAVIIYALGNKMLRFFKISNPIARGLALGTSGHALGVSAATELGETETSMASIALVLVGVVVVVIVPILTGILL

Specific function: Inhibits the expression or activity of extracellular murein hydrolases by interacting, possibly with LrgB, with the holin-like proteins CidA and/or CidB. The LrgAB and CidAB proteins may affect the proton motive force of the membrane. May be involved in p

COG id: COG1346

COG function: function code M; Putative effector of murein hydrolase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CidB/LrgB family. LrgB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788464, Length=225, Percent_Identity=37.3333333333333, Blast_Score=144, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007300 [H]

Pfam domain/function: PF04172 LrgB [H]

EC number: NA

Molecular weight: Translated: 24798; Mature: 24667

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTWLDHLGINSVYFGILLTILPFMLGQYLFKKTNGFFLFAPLFVGMVFGIAFLSITGISY
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC
DTYSKGGSIISFFLEPATICFAIPLYKKRDVLQKYWLHIIGGLSLGTAAAMLGIYGVAKL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FGFGTQIIASMLPQAATTAIALPVSAGIGGVPELTSLAVILNAVIIYALGNKMLRFFKIS
HHHHHHHHHHHCCHHHHHHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPIARGLALGTSGHALGVSAATELGETETSMASIALVLVGVVVVVIVPILTGILL
CHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TWLDHLGINSVYFGILLTILPFMLGQYLFKKTNGFFLFAPLFVGMVFGIAFLSITGISY
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCC
DTYSKGGSIISFFLEPATICFAIPLYKKRDVLQKYWLHIIGGLSLGTAAAMLGIYGVAKL
CCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
FGFGTQIIASMLPQAATTAIALPVSAGIGGVPELTSLAVILNAVIIYALGNKMLRFFKIS
HHHHHHHHHHHCCHHHHHHHEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPIARGLALGTSGHALGVSAATELGETETSMASIALVLVGVVVVVIVPILTGILL
CHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA