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The map label for this gene is dnaN [H]

Identifier: 222150252

GI number: 222150252

Start: 1859

End: 2992

Strand: Direct

Name: dnaN [H]

Synonym: MCCL_0002

Alternate gene names: 222150252

Gene position: 1859-2992 (Clockwise)

Preceding gene: 222150251

Following gene: 222150253

Centisome position: 0.09

GC content: 33.25

Gene sequence:

>1134_bases
ATGAAATTTACAATACAAAGAGACTACTTTATAGCGCAATTAAATGACACATTAAAAGCAATCTCACCAAGAACAACTTT
ACCTGTCTTAACAGGTATTAAATTAGATGTATTTGATAATCGACTTGTCTTAACAGGTTCAGATTCTGAAGTATCTATTG
AAATTACAATTCCTTCAGAACTCAATCATGAAGAAATTCTTACAGTAGAAAACAAGGGATCGATTGTATTGCCAGGTAGA
TTCTTTGTTGATATCATAAAAAAACTTCCTGCTGACACAGTAACCATTGAAACGAATGATCAGTTCCAGGCCAAAATTAC
ATCAGGTCAATCAGAGTTCAATGTTTCAGGAATTGATTCTGATCAATATCCACTTTTACCGCAAGTAAGTGAAGAAGAAG
CAATCACCTTACCTGTAAAAGTTCTTAAAAATATTATAAAGCAAACAAATTTTGCAGTGTCCACGTCAGAAACACGTCCA
GTATTAACAGGTGTTAACTGGCTGATAAGAGATAATGTATTAAACTGTACTGCAACAGATTCACATCGCTTAGCTTTACG
TAAACTTAAACTAGAAGATACGAATATCGACGAAATTAATGTCATTATTCCTGGTAAAGCTTTAAGCGAACTCAATAAAA
TTATTGGAGATAACGAAGATACGATTGATATTTTCTTTGCAGCGAACCAAGTTTTATTTAAATTTGGACATATTAACTTT
ATTTCTCGTTTGCTTGAAGGAAATTATCCAGATACATCGAGATTATTCCCTGAAATCTCAGAAACAGCACTTTCAATTAA
TAATGGTGACTTTTTCCACGCCATAGATCGTGCATCGTTATTAGCGCGTGAAGGTGGAAATAATGTTATTAAATTATCTG
CAACTGATAATACGGTAGAATTATCATCTACGTCTCCTGAAATAGGTACTGTAAAGGAAAATGTGAAAACATCTAAATTT
GATGGAACTGGAATTAAAATCTCTTTTAACTCTAAGTACATGATGGATGCGTTGCGTGCAATCGATGTTGAGGATGTACG
TGTTGAATTCTTTGGAACGATGAGACCTTTCATTCTAAAACCCGACGAAGACGATAATTTAATCCAATTAATTTTACCGA
TTAGAACATACTAA

Upstream 100 bases:

>100_bases
CATTTATCCACTTATCCACGTATTCACAGCCCCTATTACTATTATTACTATTTTAAAAAAGATATTATATACTTATAAAT
ATCAATTAAGGAGATTTACT

Downstream 100 bases:

>100_bases
AACTGAGCGATTGCTCAGTTTTTTTTGTTATATTTTGAACCTTACATGCTAAAAATTGTATAATATTGTTAGTAAATACA
ATAAAGGAGAGGTCTATGTT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 377; Mature: 377

Protein sequence:

>377_residues
MKFTIQRDYFIAQLNDTLKAISPRTTLPVLTGIKLDVFDNRLVLTGSDSEVSIEITIPSELNHEEILTVENKGSIVLPGR
FFVDIIKKLPADTVTIETNDQFQAKITSGQSEFNVSGIDSDQYPLLPQVSEEEAITLPVKVLKNIIKQTNFAVSTSETRP
VLTGVNWLIRDNVLNCTATDSHRLALRKLKLEDTNIDEINVIIPGKALSELNKIIGDNEDTIDIFFAANQVLFKFGHINF
ISRLLEGNYPDTSRLFPEISETALSINNGDFFHAIDRASLLAREGGNNVIKLSATDNTVELSSTSPEIGTVKENVKTSKF
DGTGIKISFNSKYMMDALRAIDVEDVRVEFFGTMRPFILKPDEDDNLIQLILPIRTY

Sequences:

>Translated_377_residues
MKFTIQRDYFIAQLNDTLKAISPRTTLPVLTGIKLDVFDNRLVLTGSDSEVSIEITIPSELNHEEILTVENKGSIVLPGR
FFVDIIKKLPADTVTIETNDQFQAKITSGQSEFNVSGIDSDQYPLLPQVSEEEAITLPVKVLKNIIKQTNFAVSTSETRP
VLTGVNWLIRDNVLNCTATDSHRLALRKLKLEDTNIDEINVIIPGKALSELNKIIGDNEDTIDIFFAANQVLFKFGHINF
ISRLLEGNYPDTSRLFPEISETALSINNGDFFHAIDRASLLAREGGNNVIKLSATDNTVELSSTSPEIGTVKENVKTSKF
DGTGIKISFNSKYMMDALRAIDVEDVRVEFFGTMRPFILKPDEDDNLIQLILPIRTY
>Mature_377_residues
MKFTIQRDYFIAQLNDTLKAISPRTTLPVLTGIKLDVFDNRLVLTGSDSEVSIEITIPSELNHEEILTVENKGSIVLPGR
FFVDIIKKLPADTVTIETNDQFQAKITSGQSEFNVSGIDSDQYPLLPQVSEEEAITLPVKVLKNIIKQTNFAVSTSETRP
VLTGVNWLIRDNVLNCTATDSHRLALRKLKLEDTNIDEINVIIPGKALSELNKIIGDNEDTIDIFFAANQVLFKFGHINF
ISRLLEGNYPDTSRLFPEISETALSINNGDFFHAIDRASLLAREGGNNVIKLSATDNTVELSSTSPEIGTVKENVKTSKF
DGTGIKISFNSKYMMDALRAIDVEDVRVEFFGTMRPFILKPDEDDNLIQLILPIRTY

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=375, Percent_Identity=25.8666666666667, Blast_Score=151, Evalue=6e-38,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42084; Mature: 42084

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTIQRDYFIAQLNDTLKAISPRTTLPVLTGIKLDVFDNRLVLTGSDSEVSIEITIPSE
CEEEEECCEEEEECCCHHHHCCCCCCCCEEECEEEEEECCEEEEECCCCEEEEEEEECCC
LNHEEILTVENKGSIVLPGRFFVDIIKKLPADTVTIETNDQFQAKITSGQSEFNVSGIDS
CCCCEEEEEECCCCEEECCHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEECCCC
DQYPLLPQVSEEEAITLPVKVLKNIIKQTNFAVSTSETRPVLTGVNWLIRDNVLNCTATD
CCCCCCCCCCCCCEEEEEHHHHHHHHHHCCEEEECCCCCCEECCCEEEEECCEEEEECCC
SHRLALRKLKLEDTNIDEINVIIPGKALSELNKIIGDNEDTIDIFFAANQVLFKFGHINF
CCEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCHHH
ISRLLEGNYPDTSRLFPEISETALSINNGDFFHAIDRASLLAREGGNNVIKLSATDNTVE
HHHHHCCCCCCHHHHCCCCCHHEEEECCCCEEEHHHHHHHHHHCCCCCEEEEEECCCEEE
LSSTSPEIGTVKENVKTSKFDGTGIKISFNSKYMMDALRAIDVEDVRVEFFGTMRPFILK
ECCCCCCCCCHHCCCCCCEECCCEEEEEECCHHHHHHHHHCCHHHEEEEEEECCCCEEEC
PDEDDNLIQLILPIRTY
CCCCCCEEEEEEEECCC
>Mature Secondary Structure
MKFTIQRDYFIAQLNDTLKAISPRTTLPVLTGIKLDVFDNRLVLTGSDSEVSIEITIPSE
CEEEEECCEEEEECCCHHHHCCCCCCCCEEECEEEEEECCEEEEECCCCEEEEEEEECCC
LNHEEILTVENKGSIVLPGRFFVDIIKKLPADTVTIETNDQFQAKITSGQSEFNVSGIDS
CCCCEEEEEECCCCEEECCHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCEEEEECCCC
DQYPLLPQVSEEEAITLPVKVLKNIIKQTNFAVSTSETRPVLTGVNWLIRDNVLNCTATD
CCCCCCCCCCCCCEEEEEHHHHHHHHHHCCEEEECCCCCCEECCCEEEEECCEEEEECCC
SHRLALRKLKLEDTNIDEINVIIPGKALSELNKIIGDNEDTIDIFFAANQVLFKFGHINF
CCEEEEEEEEECCCCCCEEEEEECCHHHHHHHHHHCCCCCEEEEEEECCEEEEECCCHHH
ISRLLEGNYPDTSRLFPEISETALSINNGDFFHAIDRASLLAREGGNNVIKLSATDNTVE
HHHHHCCCCCCHHHHCCCCCHHEEEECCCCEEEHHHHHHHHHHCCCCCEEEEEECCCEEE
LSSTSPEIGTVKENVKTSKFDGTGIKISFNSKYMMDALRAIDVEDVRVEFFGTMRPFILK
ECCCCCCCCCHHCCCCCCEECCCEEEEEECCHHHHHHHHHCCHHHEEEEEEECCCCEEEC
PDEDDNLIQLILPIRTY
CCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA