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The map label for this gene is rhdA [H]

Identifier: 222111246

GI number: 222111246

Start: 2201685

End: 2202638

Strand: Direct

Name: rhdA [H]

Synonym: Dtpsy_2054

Alternate gene names: 222111246

Gene position: 2201685-2202638 (Clockwise)

Preceding gene: 222111245

Following gene: 222111247

Centisome position: 57.99

GC content: 69.18

Gene sequence:

>954_bases
ATGAAAAAACTGCTCGCCCTCGCGGTATTCGCCACGGCCGCCCATGTGGCCCCTGCGGCGTGGGCAGCCCAGCCACTGCT
GACGCCCGCCGAACTGAAGGCCCAGACAACGCAGGCCACGCCGCCGCGCGTGATCGACATCCGCGACCCCGCCAGCTACG
GCGCCCAGCACATTCCGGGTGCGGTGAATGCCCCCTACGGCAAGTGGCGCGGCCCCGCCAGCAACCCGGGTGAACTACCC
GACCTGCCCCGGCTCACCGCGCTGGTGCAGTCGCTGGGGCTCACGCCCACCACGCATGCCGTGGTGGTCTCCACGGGCGC
GGACCCGACGGATTTCGGCGCTGCGGCGCGCGTGTACTGGACGCTGAAGCTGCTGGGCCTCAAGGAACTCTCCATCCTCA
ATGGCGGCATGAAGGCCTGGGGCCAAGCCCAGCTTCCGTTGACGGCCGAGGTGCCACAGGTCGCGGCCAGCCAGTACACG
CCCACGCTCGACACCCGCCTGCTGGCCACGCGCGAGGACGTGGGCCAGCACATTCGCCTGAGCAACGCCGCACTGGTGGA
TTCGCGCCCCGATGCCTTCTACCAGGGCAAGGTGCGTGCGCCGGCGGCCAAGGTGTCAGGCACCCTGCCGGGCGCACAGC
AGCTGGACTTCAACCAGTGGTTCGTTCCCGGCACCTCCACCTTCGTGGATGCGGCGACGGCCCGGCAGATCGCCGCCAAG
GTCAAGCGCGAACAGGGCCAGGACATGGTCGCGTTCTGCAATACCGGCCACTGGGCGGCCACCGACTGGTTCGGCCTGTC
CGAAGTGGCGGGCCTGCCCGGCGTGAAGCTGTATGCCGGCTCCATGGTGGACTGGACCCAGTCCCAGGATCCGCCGCTGA
TGGCGAACCAGCCCACCCGCGCCGAGGCACTGGCCTACGACGCCAAGCAGTGGTGGGAACGCAAGCTCAAGTAA

Upstream 100 bases:

>100_bases
ATCCATATTGGCTATAAGAACAATCCGATTCTGTCGTTGATGAGGCTCAAGGGCGCGGGACAATGACGCCCTTCCCTGAA
CGACTGTTTTCGAGATCGCC

Downstream 100 bases:

>100_bases
GCCAGCCCACTGCGCTGGGCACCTGACAGCTTCATAGTCAAATCGGCCTCTAGCGCTTGCCAGGCAAGCGCTAGCAGCTC
ACTTTTTTGAATCTCTAGCC

Product: rhodanese domain-containing protein

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKKLLALAVFATAAHVAPAAWAAQPLLTPAELKAQTTQATPPRVIDIRDPASYGAQHIPGAVNAPYGKWRGPASNPGELP
DLPRLTALVQSLGLTPTTHAVVVSTGADPTDFGAAARVYWTLKLLGLKELSILNGGMKAWGQAQLPLTAEVPQVAASQYT
PTLDTRLLATREDVGQHIRLSNAALVDSRPDAFYQGKVRAPAAKVSGTLPGAQQLDFNQWFVPGTSTFVDAATARQIAAK
VKREQGQDMVAFCNTGHWAATDWFGLSEVAGLPGVKLYAGSMVDWTQSQDPPLMANQPTRAEALAYDAKQWWERKLK

Sequences:

>Translated_317_residues
MKKLLALAVFATAAHVAPAAWAAQPLLTPAELKAQTTQATPPRVIDIRDPASYGAQHIPGAVNAPYGKWRGPASNPGELP
DLPRLTALVQSLGLTPTTHAVVVSTGADPTDFGAAARVYWTLKLLGLKELSILNGGMKAWGQAQLPLTAEVPQVAASQYT
PTLDTRLLATREDVGQHIRLSNAALVDSRPDAFYQGKVRAPAAKVSGTLPGAQQLDFNQWFVPGTSTFVDAATARQIAAK
VKREQGQDMVAFCNTGHWAATDWFGLSEVAGLPGVKLYAGSMVDWTQSQDPPLMANQPTRAEALAYDAKQWWERKLK
>Mature_317_residues
MKKLLALAVFATAAHVAPAAWAAQPLLTPAELKAQTTQATPPRVIDIRDPASYGAQHIPGAVNAPYGKWRGPASNPGELP
DLPRLTALVQSLGLTPTTHAVVVSTGADPTDFGAAARVYWTLKLLGLKELSILNGGMKAWGQAQLPLTAEVPQVAASQYT
PTLDTRLLATREDVGQHIRLSNAALVDSRPDAFYQGKVRAPAAKVSGTLPGAQQLDFNQWFVPGTSTFVDAATARQIAAK
VKREQGQDMVAFCNTGHWAATDWFGLSEVAGLPGVKLYAGSMVDWTQSQDPPLMANQPTRAEALAYDAKQWWERKLK

Specific function: May be a sulfotransferase involved in the transport of sulfate. Displays very low rhodanese activity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=244, Percent_Identity=26.2295081967213, Blast_Score=83, Evalue=4e-16,
Organism=Homo sapiens, GI61835204, Length=244, Percent_Identity=26.2295081967213, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI194473681, Length=244, Percent_Identity=26.2295081967213, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI17402865, Length=254, Percent_Identity=29.5275590551181, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI87082121, Length=280, Percent_Identity=24.2857142857143, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17561888, Length=271, Percent_Identity=28.0442804428044, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543836, Length=185, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17543838, Length=185, Percent_Identity=27.027027027027, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33937; Mature: 33937

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLLALAVFATAAHVAPAAWAAQPLLTPAELKAQTTQATPPRVIDIRDPASYGAQHIPG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCCHHHCHHHCCC
AVNAPYGKWRGPASNPGELPDLPRLTALVQSLGLTPTTHAVVVSTGADPTDFGAAARVYW
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHEEEE
TLKLLGLKELSILNGGMKAWGQAQLPLTAEVPQVAASQYTPTLDTRLLATREDVGQHIRL
EEEHHCHHHHHHHCCCHHHCCCCCCCEECCCHHHHHHCCCCCHHHHHHHHHHHHHCEEEE
SNAALVDSRPDAFYQGKVRAPAAKVSGTLPGAQQLDFNQWFVPGTSTFVDAATARQIAAK
CCEEEECCCCCCEECCCCCCCHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
VKREQGQDMVAFCNTGHWAATDWFGLSEVAGLPGVKLYAGSMVDWTQSQDPPLMANQPTR
HHHHCCCCEEEEECCCCEECCCCCCHHHHCCCCCCEEEECCEECCCCCCCCCCCCCCCCH
AEALAYDAKQWWERKLK
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKLLALAVFATAAHVAPAAWAAQPLLTPAELKAQTTQATPPRVIDIRDPASYGAQHIPG
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCEEEEECCCHHHCHHHCCC
AVNAPYGKWRGPASNPGELPDLPRLTALVQSLGLTPTTHAVVVSTGADPTDFGAAARVYW
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCHHHCCHHEEEE
TLKLLGLKELSILNGGMKAWGQAQLPLTAEVPQVAASQYTPTLDTRLLATREDVGQHIRL
EEEHHCHHHHHHHCCCHHHCCCCCCCEECCCHHHHHHCCCCCHHHHHHHHHHHHHCEEEE
SNAALVDSRPDAFYQGKVRAPAAKVSGTLPGAQQLDFNQWFVPGTSTFVDAATARQIAAK
CCEEEECCCCCCEECCCCCCCHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHH
VKREQGQDMVAFCNTGHWAATDWFGLSEVAGLPGVKLYAGSMVDWTQSQDPPLMANQPTR
HHHHCCCCEEEEECCCCEECCCCCCHHHHCCCCCCEEEECCEECCCCCCCCCCCCCCCCH
AEALAYDAKQWWERKLK
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1708376 [H]