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The map label for this gene is thiE [H]

Identifier: 222111131

GI number: 222111131

Start: 2073098

End: 2074027

Strand: Direct

Name: thiE [H]

Synonym: Dtpsy_1939

Alternate gene names: 222111131

Gene position: 2073098-2074027 (Clockwise)

Preceding gene: 222111130

Following gene: 222111133

Centisome position: 54.6

GC content: 70.65

Gene sequence:

>930_bases
ATGCCGCAAGCCGCCGCGCCATTGCCTTCTGCAGCCGCCATGGAGCAAGCCATCCGCCAGCACCATGCGTCGGCGTTCGC
GGACTTCCCGCCCCAGCCGATGCCGGCGACAACCGTGGAAGACCCCGTGTACCGCGCAGCCCTTGCGGCCTGCAGCGCCC
TGGGTTTCATCGCCCATGACGCAGACTGCCTGGCCCGAGCCTGGGCTGCGCAGACGCGGCGCACCGGCCGCTTCGACCCG
GCGCAGTGGCCCGACGAGCCCGCAGACTTCGGACTGCAGCCGCGCCCCCATGCGCACCCCTTTGCAGCCTGCCCGCAGAA
CCTGGGTCTCTACGCCGTATTGCCCGATGCCCAATGGGTGGGCCGCATGGCGCAGGCCGGCGTGCCGACGGTGCAGCTGC
GCTTCAAAAGTGACGACCCTGCTGCCATCACACGCGAGGTACGCGCCGCAGTGCAGGCCGTGCAAGGCACCGCTTCGCTG
CTGTTCATCAACGACCATTGGCGCCAGGCCATCGCGGCCGGGGCCTATGGCGTGCACCTGGGCCAGGAGGATCTGGATGC
GCTGAGCCCCGAAGAGCTGCGCACGCTGCGCGAGTCCGGGGTGCGCCTGGGCGTGAGCACGCATGGCTACGCCGAGATGG
TGCGCGCCGACGCGGCCAGCCCCAGCTACATCGCCATGGGCGCGGTGTTCCCCACCACGCTCAAGAAAATGGCCACAGTG
CCCCAGGGCGTGGCGCGGCTGGCGGGCTATGCCCGGCTGATGCGCGGCTATCCGCTGGTTGCCATCGGCGGCATTGGCCT
GGCCCAGTTCCCGCAAGTGCTGGCCACCGGAGTGGGATCGATCGCCGTGGTGCGCGCCGTGGTGGCGGCCGATCAGCCGG
AAGCCGCTGCAGCGCAGCTCATGCGGGCCATGGACGCCGGCAGGCGATAG

Upstream 100 bases:

>100_bases
GCGCCGCCCGCCTGGCCGGCGCGATGACGCCGCAGGAGGCGGCGCAACCCAGCACGCCCGTGCTGGGCACGCCGTTCTGG
CACCACGCGCAGGGCTGAGC

Downstream 100 bases:

>100_bases
CCCCGTCCGCGCATGGGGGGCGTTCTCGCTTCAGAAGCGGTTCTTTTGCGACTGCTCCAGTTCGAAGCGCAACTCCAGCT
TGTCGTTTTCCATGCCAAAA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MPQAAAPLPSAAAMEQAIRQHHASAFADFPPQPMPATTVEDPVYRAALAACSALGFIAHDADCLARAWAAQTRRTGRFDP
AQWPDEPADFGLQPRPHAHPFAACPQNLGLYAVLPDAQWVGRMAQAGVPTVQLRFKSDDPAAITREVRAAVQAVQGTASL
LFINDHWRQAIAAGAYGVHLGQEDLDALSPEELRTLRESGVRLGVSTHGYAEMVRADAASPSYIAMGAVFPTTLKKMATV
PQGVARLAGYARLMRGYPLVAIGGIGLAQFPQVLATGVGSIAVVRAVVAADQPEAAAAQLMRAMDAGRR

Sequences:

>Translated_309_residues
MPQAAAPLPSAAAMEQAIRQHHASAFADFPPQPMPATTVEDPVYRAALAACSALGFIAHDADCLARAWAAQTRRTGRFDP
AQWPDEPADFGLQPRPHAHPFAACPQNLGLYAVLPDAQWVGRMAQAGVPTVQLRFKSDDPAAITREVRAAVQAVQGTASL
LFINDHWRQAIAAGAYGVHLGQEDLDALSPEELRTLRESGVRLGVSTHGYAEMVRADAASPSYIAMGAVFPTTLKKMATV
PQGVARLAGYARLMRGYPLVAIGGIGLAQFPQVLATGVGSIAVVRAVVAADQPEAAAAQLMRAMDAGRR
>Mature_308_residues
PQAAAPLPSAAAMEQAIRQHHASAFADFPPQPMPATTVEDPVYRAALAACSALGFIAHDADCLARAWAAQTRRTGRFDPA
QWPDEPADFGLQPRPHAHPFAACPQNLGLYAVLPDAQWVGRMAQAGVPTVQLRFKSDDPAAITREVRAAVQAVQGTASLL
FINDHWRQAIAAGAYGVHLGQEDLDALSPEELRTLRESGVRLGVSTHGYAEMVRADAASPSYIAMGAVFPTTLKKMATVP
QGVARLAGYARLMRGYPLVAIGGIGLAQFPQVLATGVGSIAVVRAVVAADQPEAAAAQLMRAMDAGRR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=207, Percent_Identity=48.792270531401, Blast_Score=172, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 32643; Mature: 32511

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQAAAPLPSAAAMEQAIRQHHASAFADFPPQPMPATTVEDPVYRAALAACSALGFIAHD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ADCLARAWAAQTRRTGRFDPAQWPDEPADFGLQPRPHAHPFAACPQNLGLYAVLPDAQWV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEECCHHHH
GRMAQAGVPTVQLRFKSDDPAAITREVRAAVQAVQGTASLLFINDHWRQAIAAGAYGVHL
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCHHHEEC
GQEDLDALSPEELRTLRESGVRLGVSTHGYAEMVRADAASPSYIAMGAVFPTTLKKMATV
CHHHHHCCCHHHHHHHHHCCCEECCCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
PQGVARLAGYARLMRGYPLVAIGGIGLAQFPQVLATGVGSIAVVRAVVAADQPEAAAAQL
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
MRAMDAGRR
HHHHHCCCC
>Mature Secondary Structure 
PQAAAPLPSAAAMEQAIRQHHASAFADFPPQPMPATTVEDPVYRAALAACSALGFIAHD
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ADCLARAWAAQTRRTGRFDPAQWPDEPADFGLQPRPHAHPFAACPQNLGLYAVLPDAQWV
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEECCHHHH
GRMAQAGVPTVQLRFKSDDPAAITREVRAAVQAVQGTASLLFINDHWRQAIAAGAYGVHL
HHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHCHHHEEC
GQEDLDALSPEELRTLRESGVRLGVSTHGYAEMVRADAASPSYIAMGAVFPTTLKKMATV
CHHHHHCCCHHHHHHHHHCCCEECCCCCHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
PQGVARLAGYARLMRGYPLVAIGGIGLAQFPQVLATGVGSIAVVRAVVAADQPEAAAAQL
HHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHH
MRAMDAGRR
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA