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The map label for this gene is cysNC [H]

Identifier: 222111123

GI number: 222111123

Start: 2066100

End: 2067488

Strand: Direct

Name: cysNC [H]

Synonym: Dtpsy_1931

Alternate gene names: 222111123

Gene position: 2066100-2067488 (Clockwise)

Preceding gene: 222111122

Following gene: 222111124

Centisome position: 54.42

GC content: 69.91

Gene sequence:

>1389_bases
ATGACCACCGCCACTATCAATTCAATAGCTTCCAGCGCACAACCAGAAAGCGCTGGCGGCACAAATGATCACATTTCCGC
GCTGCGCTTCATCACCTGCGGCAGCGTGGACGATGGCAAGAGCACGCTGATCGGCCGCCTGCTGGTGGACAGCCGCGCCG
TGCTGCAGGACCACTTGGCCGGGGTGCAGCGCGGCGGAGAGACCGACCTGGCGCTGCTGACCGACGGCCTCAGTGCCGAG
CGCGAGCAGGGCATCACCATCGACGTGGCCTACCGCTACTTCGCCACCGAGGCGCGCAAGTTCATCATCGGCGACGCGCC
CGGCCACGAACAGTACACGCGCAACATGGTCACCGCCGCCTCCCAGGCCGACGCCGCCGTGGTGCTGGTGGACGCCACCA
AGCTGGATTGGCAGAACCCGCAACTCACGCTGCTGCCGCAAACACGCCGCCACAGCCTGCTGGTGCACCTGCTGCGTGTG
CATTCGCTGGTGTTTGCCGTGAACAAGCTCGACGCCGTGGCCGACCCACAGCTCGCCTACCGTCACATCCGTGCCGCGCT
GGAGCAGTTCGCCCGACACGCGGGCATCGCGGTGGCGGGCGTGGTGCCGGTGTCGGCGCTCAAGGGCTGGAACGTGGTAG
AGGCCAAGCCCGGCTGGTGCGACTACGCAGGCCCGAGCCTGCTGCAGATCCTGGAGCGGCTGCCCAACACCCCGCCCGAC
GCCGGCCTGCCCCTGGCCTTCCCGGTGCAGTGGGTGGAGAAATTTTCTTCCTCGTCCGACACTTCGCAGGGGCGCCGCGT
GTTCTGGGGCCGGGTGGGCACGGGCCGCGTGCAGCCAGGTGTGCAGGTCGCCATCTTCCCCAGCGGCCAGCTGGCGCAGG
TGGCGCAGGTGCTGGACCATGCGCGCCGCGCCACCGGCGTGGCCGCGGGCCAGAGCGCGGGCATCACCCTCGACCGCGAG
GTGGACGTCTCGCGCGGCGACTGGATCGTGGCTGCGCCCGCACCCGCTGCCGCGGCCGAGGACGACTTCGACACCCCCGC
CACCGTGCCCGCATGGCCCGGCCAGCGCGAGATCACCGCCACCGTCGCGTGGATGGACGACGAGCCGCTGGTGGCGGGCC
GGGTGTACTGGGCGCTGCATGGCCACCGCTGGGTCAAGGTCAAGGTGCGCCGCGTGGTCCACCGCCTGGACATCAACACC
CTGGCCGAGGAAGACGCCACCCAGCTGGAGCCCAATGCCATCGGCCATGTGGAGCTGCTGCTGCAGGAACCCCTGCCCGC
GGCGCCGTTCGCGCGCAGCCGCGTGCTCGGTTCGCTCATCCTGGTCGACACCGCCAGCCACAAGACGGCGGGCGCCGTGC
TGGTGAACGCACCGGCCCCAAAAGCTTGA

Upstream 100 bases:

>100_bases
AGACCCTGGCCGCCGAGGTCAGCGAGCGCGGCGCCACGCGCATGGACGACAAGACCAGCGACGCCTCGATGGAAAAGCGC
AAGAAAGACGGGTATTTCTG

Downstream 100 bases:

>100_bases
TCAACGTTAAGGTTTCCGCGCCTCAGGCATGGTTGGCTGGCCTGCTGAGGCGGCGGTCGGCCTAAAATCGCGGGCTAACC
CCATACCAGAACACAACTAC

Product: sulfate adenylyltransferase large subunit

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTTATINSIASSAQPESAGGTNDHISALRFITCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQRGGETDLALLTDGLSAE
REQGITIDVAYRYFATEARKFIIGDAPGHEQYTRNMVTAASQADAAVVLVDATKLDWQNPQLTLLPQTRRHSLLVHLLRV
HSLVFAVNKLDAVADPQLAYRHIRAALEQFARHAGIAVAGVVPVSALKGWNVVEAKPGWCDYAGPSLLQILERLPNTPPD
AGLPLAFPVQWVEKFSSSSDTSQGRRVFWGRVGTGRVQPGVQVAIFPSGQLAQVAQVLDHARRATGVAAGQSAGITLDRE
VDVSRGDWIVAAPAPAAAAEDDFDTPATVPAWPGQREITATVAWMDDEPLVAGRVYWALHGHRWVKVKVRRVVHRLDINT
LAEEDATQLEPNAIGHVELLLQEPLPAAPFARSRVLGSLILVDTASHKTAGAVLVNAPAPKA

Sequences:

>Translated_462_residues
MTTATINSIASSAQPESAGGTNDHISALRFITCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQRGGETDLALLTDGLSAE
REQGITIDVAYRYFATEARKFIIGDAPGHEQYTRNMVTAASQADAAVVLVDATKLDWQNPQLTLLPQTRRHSLLVHLLRV
HSLVFAVNKLDAVADPQLAYRHIRAALEQFARHAGIAVAGVVPVSALKGWNVVEAKPGWCDYAGPSLLQILERLPNTPPD
AGLPLAFPVQWVEKFSSSSDTSQGRRVFWGRVGTGRVQPGVQVAIFPSGQLAQVAQVLDHARRATGVAAGQSAGITLDRE
VDVSRGDWIVAAPAPAAAAEDDFDTPATVPAWPGQREITATVAWMDDEPLVAGRVYWALHGHRWVKVKVRRVVHRLDINT
LAEEDATQLEPNAIGHVELLLQEPLPAAPFARSRVLGSLILVDTASHKTAGAVLVNAPAPKA
>Mature_461_residues
TTATINSIASSAQPESAGGTNDHISALRFITCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQRGGETDLALLTDGLSAER
EQGITIDVAYRYFATEARKFIIGDAPGHEQYTRNMVTAASQADAAVVLVDATKLDWQNPQLTLLPQTRRHSLLVHLLRVH
SLVFAVNKLDAVADPQLAYRHIRAALEQFARHAGIAVAGVVPVSALKGWNVVEAKPGWCDYAGPSLLQILERLPNTPPDA
GLPLAFPVQWVEKFSSSSDTSQGRRVFWGRVGTGRVQPGVQVAIFPSGQLAQVAQVLDHARRATGVAAGQSAGITLDREV
DVSRGDWIVAAPAPAAAAEDDFDTPATVPAWPGQREITATVAWMDDEPLVAGRVYWALHGHRWVKVKVRRVVHRLDINTL
AEEDATQLEPNAIGHVELLLQEPLPAAPFARSRVLGSLILVDTASHKTAGAVLVNAPAPKA

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=30.5084745762712, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503471, Length=326, Percent_Identity=31.2883435582822, Blast_Score=126, Evalue=5e-29,
Organism=Homo sapiens, GI5729864, Length=303, Percent_Identity=30.03300330033, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI223555963, Length=303, Percent_Identity=30.03300330033, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=345, Percent_Identity=28.4057971014493, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194018520, Length=310, Percent_Identity=29.3548387096774, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI194097354, Length=310, Percent_Identity=29.3548387096774, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI194018522, Length=340, Percent_Identity=28.5294117647059, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI34147630, Length=163, Percent_Identity=33.7423312883436, Blast_Score=76, Evalue=8e-14,
Organism=Escherichia coli, GI1789108, Length=471, Percent_Identity=38.8535031847134, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1790412, Length=140, Percent_Identity=33.5714285714286, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1789737, Length=140, Percent_Identity=33.5714285714286, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=28.5398230088496, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=283, Percent_Identity=34.2756183745583, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=283, Percent_Identity=34.2756183745583, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17552884, Length=354, Percent_Identity=29.9435028248588, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI17569207, Length=354, Percent_Identity=29.9435028248588, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=352, Percent_Identity=26.9886363636364, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=143, Percent_Identity=37.7622377622378, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=309, Percent_Identity=25.5663430420712, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=168, Percent_Identity=32.7380952380952, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322937, Length=419, Percent_Identity=29.5942720763723, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6325337, Length=354, Percent_Identity=29.9435028248588, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6319594, Length=354, Percent_Identity=29.9435028248588, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320377, Length=401, Percent_Identity=24.1895261845387, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324761, Length=317, Percent_Identity=30.2839116719243, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45553807, Length=362, Percent_Identity=31.767955801105, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45553816, Length=362, Percent_Identity=31.767955801105, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24651721, Length=362, Percent_Identity=31.767955801105, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17864154, Length=362, Percent_Identity=31.767955801105, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550900, Length=308, Percent_Identity=32.7922077922078, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652838, Length=362, Percent_Identity=31.767955801105, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137572, Length=362, Percent_Identity=31.767955801105, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137380, Length=331, Percent_Identity=28.3987915407855, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281363316, Length=257, Percent_Identity=32.6848249027237, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI17864358, Length=257, Percent_Identity=32.6848249027237, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19921738, Length=225, Percent_Identity=27.5555555555556, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 49476; Mature: 49344

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTATINSIASSAQPESAGGTNDHISALRFITCGSVDDGKSTLIGRLLVDSRAVLQDHLA
CCCCCHHHHHHCCCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
GVQRGGETDLALLTDGLSAEREQGITIDVAYRYFATEARKFIIGDAPGHEQYTRNMVTAA
HHHCCCCCCEEEEECCCCCHHHCCCEEEEEHHHHHHHCCEEEEECCCCHHHHHHHHHHHC
SQADAAVVLVDATKLDWQNPQLTLLPQTRRHSLLVHLLRVHSLVFAVNKLDAVADPQLAY
CCCCEEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RHIRAALEQFARHAGIAVAGVVPVSALKGWNVVEAKPGWCDYAGPSLLQILERLPNTPPD
HHHHHHHHHHHHHCCEEEEECHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCC
AGLPLAFPVQWVEKFSSSSDTSQGRRVFWGRVGTGRVQPGVQVAIFPSGQLAQVAQVLDH
CCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHH
ARRATGVAAGQSAGITLDREVDVSRGDWIVAAPAPAAAAEDDFDTPATVPAWPGQREITA
HHHHHCCCCCCCCCEEEECEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEE
TVAWMDDEPLVAGRVYWALHGHRWVKVKVRRVVHRLDINTLAEEDATQLEPNAIGHVELL
EEEEECCCCCEEEEEEEEEECCEEEEHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCEEEE
LQEPLPAAPFARSRVLGSLILVDTASHKTAGAVLVNAPAPKA
ECCCCCCCCCHHHHHHCEEEEEECCCCCCCCEEEEECCCCCC
>Mature Secondary Structure 
TTATINSIASSAQPESAGGTNDHISALRFITCGSVDDGKSTLIGRLLVDSRAVLQDHLA
CCCCHHHHHHCCCCCCCCCCCHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
GVQRGGETDLALLTDGLSAEREQGITIDVAYRYFATEARKFIIGDAPGHEQYTRNMVTAA
HHHCCCCCCEEEEECCCCCHHHCCCEEEEEHHHHHHHCCEEEEECCCCHHHHHHHHHHHC
SQADAAVVLVDATKLDWQNPQLTLLPQTRRHSLLVHLLRVHSLVFAVNKLDAVADPQLAY
CCCCEEEEEEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RHIRAALEQFARHAGIAVAGVVPVSALKGWNVVEAKPGWCDYAGPSLLQILERLPNTPPD
HHHHHHHHHHHHHCCEEEEECHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHCCCCCCC
AGLPLAFPVQWVEKFSSSSDTSQGRRVFWGRVGTGRVQPGVQVAIFPSGQLAQVAQVLDH
CCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCEEEEECCCCHHHHHHHHHH
ARRATGVAAGQSAGITLDREVDVSRGDWIVAAPAPAAAAEDDFDTPATVPAWPGQREITA
HHHHHCCCCCCCCCEEEECEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEE
TVAWMDDEPLVAGRVYWALHGHRWVKVKVRRVVHRLDINTLAEEDATQLEPNAIGHVELL
EEEEECCCCCEEEEEEEEEECCEEEEHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCEEEE
LQEPLPAAPFARSRVLGSLILVDTASHKTAGAVLVNAPAPKA
ECCCCCCCCCHHHHHHCEEEEEECCCCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]