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The map label for this gene is mnmC [H]

Identifier: 222111118

GI number: 222111118

Start: 2060187

End: 2062106

Strand: Direct

Name: mnmC [H]

Synonym: Dtpsy_1926

Alternate gene names: 222111118

Gene position: 2060187-2062106 (Clockwise)

Preceding gene: 222111117

Following gene: 222111119

Centisome position: 54.26

GC content: 72.14

Gene sequence:

>1920_bases
ATGTCCGAACCGATTGAATGGCTGGAAGACGGCACGCCCTACAGCCCGCGCTTCTCAGACCGGTACCACAGCGAGCAAGG
CGGCCTGGAACAAGCCCGCGGCACATTTCTCGCCGGCTGCGGCCTGCCCCAGGCCTGGCAGAACCAGCCGCAGTGGCGCG
TGCTGGAAACCGGCTTCGGCCTGGGCCTGAATTTCCTCGTGACCTGGGCCGCCTGGCGCGCCGACCCCCACCGGCCACAG
CGGCTGCACTTCGTGTCTTGCGAGGCCTGGCCCGTGAGCGCGGCCGATCTGCTGCGCGCCGCGCCCATCGATTCCACACT
GCAATCGCTGGCCCAACAGTTGGCGGCGCAGTACTGGGGCTTGCTGCCGGGCGTGCACAGGCTCTCGTTCGACGGCGGGC
AGGTGCTGCTCACGCTGTACATCGGTGACGCGCAAGCCCTGCTGAGGCAGCAGCAGCCCACGGCGGATTCGGTGTACCTG
GACGGCTTCGACCCCCAGCACAACCCGCAAATGTGGGACATCCACACCCTCAAGGCCGTGGCGCGCTGCTGCCGTCGCGG
CACCCGCCTGGCGACGTGGACGGTGGCGCGCGGCGTGCGGGACGGGCTGGTGCAGTGCGGCTTCGAGGTGCAAAAGGTGC
CGGGTGTCCGCCCCAAGCGCGACAACCTGCAGGCCACCTACGCGCCCCGCTGGGAGCCACGCGCCGGCCAGCCCGTGCTG
CCAGACGCCACGGTGGCCGCCCCGGCCCGCTGCCTGGTGGTGGGCGCAGGACTGGCGGGCGCCGCCGTGGCGGCCAGCCT
GGCGCACCGTGGCTGGCAGGTCCAGGTGCTGGACCAGGCCGATCACCCTGCCGCCGGTGCATCCGGCCTGCCGGCGGGGG
TGTTCGCGCCCAATGTCTCGCAGGACGACAACCTGATCTCACGCCTGTCGCGCGCGGGGGTACGCACCACGCTGAATACG
CTGGCGCAGCTTCCAGCCGAGACGCGCGGCACCGACTGGAGCGTGTGCGGCACGCTGGAGCACCGTGTGGACGGCACCAC
AGGATTGGCCTGGAGCGACGGGCCTGGCCTGGACTGGAGTCGCCCCGCAACACCCGCACAACTGCAGGCCAACGGCTTAT
CGGCAGACACGGTGGCCTGCTGGCACGAGCACGCGGGCTGGGTGCGCCCGCCCCAGCTCATCGCCCACCTGCTGAACGAC
CCGGCCATCCACTGGCGCGGCAGCGCGGCAGTGGCGGAGTTGCGCCGCACCACCGTCGACGGCCAGCCCCTCTGGCAGGC
ACTGGACGCCGAAGGGCGCGTCCTGGCCGAGGCGGAACTTGCCGTGGTTTGCGCCGGGCACCACACCGGCCCGCTCACGC
ACGCGCATTGGCCCATGAATCCGCTGCGCGGCCAGTTGGCCTGGGGCCTGCATGCCCAGGCTCCCGCGGGCGCCCCCTGG
CCGCCCCAGCCGGTCAATGGCAACGGCAACCTGGTGCCCCGTGTGCCGCTGCAGGGTGGCGCAGGCTGGGTGCTGGGCTC
CACGTTCGAGCGCCTGAAGACCGCCCTGCCCCCCAGCCCTGACGACCAAGCCAAGGGCTTGGCCACCAATCAGGCCAAAC
TGCACGCGCTGCTGCCCCCCCTGGGCGTTGCCATGGACGCCGCCTTCACCCAGGCCGTCACCGCGCCGGACGGCCCCGTG
CGCACCTGGGCGGCGGTGCGCTGCGGTGCGCTGGACCGCCGCCCCATCGTGGGGCCGGTGGACTCGGTGCGCCAGCCCGG
CCTGTGGCTGTGCACTGCCATGGGCGCGCGCGGGCTCACGCTGTCATTGCTGTGCGGCGAACTGATCGCCGCGCGGCTGC
ACAGTGAGCCCCTGCCGCTGGACGCCCCTCTGGCGCGCGCGTTGAGCAGCGAGCGCTGGCTAGGATATGAGCCACAGTGA

Upstream 100 bases:

>100_bases
GCTACTGGCACTGCACTACCACTGGCGCCTGCCGGTGTTCTCGTACCAGCAGCGCTGGGAATGACACTGTTTCTGTAATC
TTTTCGCGCCTCCCAACACC

Downstream 100 bases:

>100_bases
GCCGACACGCATTTCATATACAAATAACGGAATATATGAAGCATTAAAAAATAGTTTGTCTTTATAAGACAGACTTTTAA
AGTGCGCCCATCGATGCTGC

Product: tRNA u-34 5-methylaminomethyl-2-thiouridine biosynthesis protein mnmc

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSEPIEWLEDGTPYSPRFSDRYHSEQGGLEQARGTFLAGCGLPQAWQNQPQWRVLETGFGLGLNFLVTWAAWRADPHRPQ
RLHFVSCEAWPVSAADLLRAAPIDSTLQSLAQQLAAQYWGLLPGVHRLSFDGGQVLLTLYIGDAQALLRQQQPTADSVYL
DGFDPQHNPQMWDIHTLKAVARCCRRGTRLATWTVARGVRDGLVQCGFEVQKVPGVRPKRDNLQATYAPRWEPRAGQPVL
PDATVAAPARCLVVGAGLAGAAVAASLAHRGWQVQVLDQADHPAAGASGLPAGVFAPNVSQDDNLISRLSRAGVRTTLNT
LAQLPAETRGTDWSVCGTLEHRVDGTTGLAWSDGPGLDWSRPATPAQLQANGLSADTVACWHEHAGWVRPPQLIAHLLND
PAIHWRGSAAVAELRRTTVDGQPLWQALDAEGRVLAEAELAVVCAGHHTGPLTHAHWPMNPLRGQLAWGLHAQAPAGAPW
PPQPVNGNGNLVPRVPLQGGAGWVLGSTFERLKTALPPSPDDQAKGLATNQAKLHALLPPLGVAMDAAFTQAVTAPDGPV
RTWAAVRCGALDRRPIVGPVDSVRQPGLWLCTAMGARGLTLSLLCGELIAARLHSEPLPLDAPLARALSSERWLGYEPQ

Sequences:

>Translated_639_residues
MSEPIEWLEDGTPYSPRFSDRYHSEQGGLEQARGTFLAGCGLPQAWQNQPQWRVLETGFGLGLNFLVTWAAWRADPHRPQ
RLHFVSCEAWPVSAADLLRAAPIDSTLQSLAQQLAAQYWGLLPGVHRLSFDGGQVLLTLYIGDAQALLRQQQPTADSVYL
DGFDPQHNPQMWDIHTLKAVARCCRRGTRLATWTVARGVRDGLVQCGFEVQKVPGVRPKRDNLQATYAPRWEPRAGQPVL
PDATVAAPARCLVVGAGLAGAAVAASLAHRGWQVQVLDQADHPAAGASGLPAGVFAPNVSQDDNLISRLSRAGVRTTLNT
LAQLPAETRGTDWSVCGTLEHRVDGTTGLAWSDGPGLDWSRPATPAQLQANGLSADTVACWHEHAGWVRPPQLIAHLLND
PAIHWRGSAAVAELRRTTVDGQPLWQALDAEGRVLAEAELAVVCAGHHTGPLTHAHWPMNPLRGQLAWGLHAQAPAGAPW
PPQPVNGNGNLVPRVPLQGGAGWVLGSTFERLKTALPPSPDDQAKGLATNQAKLHALLPPLGVAMDAAFTQAVTAPDGPV
RTWAAVRCGALDRRPIVGPVDSVRQPGLWLCTAMGARGLTLSLLCGELIAARLHSEPLPLDAPLARALSSERWLGYEPQ
>Mature_638_residues
SEPIEWLEDGTPYSPRFSDRYHSEQGGLEQARGTFLAGCGLPQAWQNQPQWRVLETGFGLGLNFLVTWAAWRADPHRPQR
LHFVSCEAWPVSAADLLRAAPIDSTLQSLAQQLAAQYWGLLPGVHRLSFDGGQVLLTLYIGDAQALLRQQQPTADSVYLD
GFDPQHNPQMWDIHTLKAVARCCRRGTRLATWTVARGVRDGLVQCGFEVQKVPGVRPKRDNLQATYAPRWEPRAGQPVLP
DATVAAPARCLVVGAGLAGAAVAASLAHRGWQVQVLDQADHPAAGASGLPAGVFAPNVSQDDNLISRLSRAGVRTTLNTL
AQLPAETRGTDWSVCGTLEHRVDGTTGLAWSDGPGLDWSRPATPAQLQANGLSADTVACWHEHAGWVRPPQLIAHLLNDP
AIHWRGSAAVAELRRTTVDGQPLWQALDAEGRVLAEAELAVVCAGHHTGPLTHAHWPMNPLRGQLAWGLHAQAPAGAPWP
PQPVNGNGNLVPRVPLQGGAGWVLGSTFERLKTALPPSPDDQAKGLATNQAKLHALLPPLGVAMDAAFTQAVTAPDGPVR
TWAAVRCGALDRRPIVGPVDSVRQPGLWLCTAMGARGLTLSLLCGELIAARLHSEPLPLDAPLARALSSERWLGYEPQ

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=674, Percent_Identity=29.080118694362, Blast_Score=211, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 68586; Mature: 68455

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPIEWLEDGTPYSPRFSDRYHSEQGGLEQARGTFLAGCGLPQAWQNQPQWRVLETGFG
CCCCHHHHHCCCCCCCCCCHHHCCCCCCHHHHCCCEEECCCCCHHHCCCCCEEEEECCCC
LGLNFLVTWAAWRADPHRPQRLHFVSCEAWPVSAADLLRAAPIDSTLQSLAQQLAAQYWG
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LLPGVHRLSFDGGQVLLTLYIGDAQALLRQQQPTADSVYLDGFDPQHNPQMWDIHTLKAV
HCCCCCEEECCCCEEEEEEEECCHHHHHHCCCCCCCEEEECCCCCCCCCCEEEHHHHHHH
ARCCRRGTRLATWTVARGVRDGLVQCGFEVQKVPGVRPKRDNLQATYAPRWEPRAGQPVL
HHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCC
PDATVAAPARCLVVGAGLAGAAVAASLAHRGWQVQVLDQADHPAAGASGLPAGVFAPNVS
CCCHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
QDDNLISRLSRAGVRTTLNTLAQLPAETRGTDWSVCGTLEHRVDGTTGLAWSDGPGLDWS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCC
RPATPAQLQANGLSADTVACWHEHAGWVRPPQLIAHLLNDPAIHWRGSAAVAELRRTTVD
CCCCCCEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHCCC
GQPLWQALDAEGRVLAEAELAVVCAGHHTGPLTHAHWPMNPLRGQLAWGLHAQAPAGAPW
CCHHHHHHCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
PPQPVNGNGNLVPRVPLQGGAGWVLGSTFERLKTALPPSPDDQAKGLATNQAKLHALLPP
CCCCCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHCCC
LGVAMDAAFTQAVTAPDGPVRTWAAVRCGALDRRPIVGPVDSVRQPGLWLCTAMGARGLT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCC
LSLLCGELIAARLHSEPLPLDAPLARALSSERWLGYEPQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SEPIEWLEDGTPYSPRFSDRYHSEQGGLEQARGTFLAGCGLPQAWQNQPQWRVLETGFG
CCCHHHHHCCCCCCCCCCHHHCCCCCCHHHHCCCEEECCCCCHHHCCCCCEEEEECCCC
LGLNFLVTWAAWRADPHRPQRLHFVSCEAWPVSAADLLRAAPIDSTLQSLAQQLAAQYWG
CCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
LLPGVHRLSFDGGQVLLTLYIGDAQALLRQQQPTADSVYLDGFDPQHNPQMWDIHTLKAV
HCCCCCEEECCCCEEEEEEEECCHHHHHHCCCCCCCEEEECCCCCCCCCCEEEHHHHHHH
ARCCRRGTRLATWTVARGVRDGLVQCGFEVQKVPGVRPKRDNLQATYAPRWEPRAGQPVL
HHHHHCCCCEEEHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCC
PDATVAAPARCLVVGAGLAGAAVAASLAHRGWQVQVLDQADHPAAGASGLPAGVFAPNVS
CCCHHHCCCEEEEEECCHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCC
QDDNLISRLSRAGVRTTLNTLAQLPAETRGTDWSVCGTLEHRVDGTTGLAWSDGPGLDWS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCC
RPATPAQLQANGLSADTVACWHEHAGWVRPPQLIAHLLNDPAIHWRGSAAVAELRRTTVD
CCCCCCEEECCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCEEECCHHHHHHHHHHCCC
GQPLWQALDAEGRVLAEAELAVVCAGHHTGPLTHAHWPMNPLRGQLAWGLHAQAPAGAPW
CCHHHHHHCCCCCEEEECEEEEEEECCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCC
PPQPVNGNGNLVPRVPLQGGAGWVLGSTFERLKTALPPSPDDQAKGLATNQAKLHALLPP
CCCCCCCCCCEEEECCCCCCCCEEHHHHHHHHHHHCCCCCCHHHCCCCCCCHHHHHHCCC
LGVAMDAAFTQAVTAPDGPVRTWAAVRCGALDRRPIVGPVDSVRQPGLWLCTAMGARGLT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEEECCCCCCC
LSLLCGELIAARLHSEPLPLDAPLARALSSERWLGYEPQ
HHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA