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The map label for this gene is fabI [H]

Identifier: 222111112

GI number: 222111112

Start: 2053725

End: 2054525

Strand: Direct

Name: fabI [H]

Synonym: Dtpsy_1919

Alternate gene names: 222111112

Gene position: 2053725-2054525 (Clockwise)

Preceding gene: 222111105

Following gene: 222111116

Centisome position: 54.09

GC content: 65.29

Gene sequence:

>801_bases
ATGGGTTTTCTCGCCGGCAAGAAGCTGCTCATCACGGGCGTGCTGTCCAATCGTTCGATCGCGTACGGCATCGCCAGGGC
CTGCCACCAACAAGGCGCGGAACTGGCCTTCAGCTACGTGGGCGAGCGCTTCAAGGACCGCATCACCGAGTTCGCCGCCG
AATTCGACTCCAAGCTGGTCTTTGACTGCGACGTGGCCGACGACGCGCAGATCGACAAATTGTTCGCCGACATCGGCGCC
GCCTGGGGCAAGTTCGATGGCTTCGTGCACTCCATCGGCTTTGCTCCGCGCGAGGCCATCGCAGGCAACTTCCTGGAAGG
CCTCTCTCGCGAAGCCTTCAAGGTGGCCCACGACATCAGTGCCTACAGCTTCCCGGCCATGGCCAAGGCGGCCCTGCCCT
ACCTGAACGACAAGTCGTCGCTGCTGACGCTGTCGTACCTGGGCGCCCTGCGCTCCATCCCCAACTACAACACCATGGGC
CTGGCCAAGGCCAGCCTGGAAGCCAGCGTGCGCTACCTGGCAGAAGCGGTGGGCCGCACTGAAGACGGCCGCTGCATCCG
TGCCAACGGCATCAGCGCGGGCCCCATCAAGACGCTGGCCGCCAGCGGCATCAAGGACTTCGGCAAGCTGTTGGGCCGCG
TGGCTGATGCCGCCCCGCTGCGCCGCAACGTGACGATCGAAGACGTGGGCAACGTGGCCGCGTTCCTGCTGTCCGACCTG
GCCAGCGGCGTGACCGCCGAGATCACCTATGTGGACGGCGGCTTCAGCCAAACCGCCGGGCTTTCGGCAGACCAGGTGTG
A

Upstream 100 bases:

>100_bases
CAGAACCGCATGCGACAATTCTGCACCACGCAAGCGGCCCGCAAGCCCCCGCGCCACGCTTGCCATTTCCCTTCGCACAT
CGCATTTCAGGAGAACAACA

Downstream 100 bases:

>100_bases
GCCTGCGGCAATGACTGCGGAGTGGGTGCGCCACACCGCCCTTGCTTGAAGAACCAACGGGCCTGCAAGGCCCGTTGTTC
GTTTCGGTCACTGTGCGCGG

Product: short-chain dehydrogenase/reductase sdr

Products: NA

Alternate protein names: NADH-dependent enoyl-ACP reductase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MGFLAGKKLLITGVLSNRSIAYGIARACHQQGAELAFSYVGERFKDRITEFAAEFDSKLVFDCDVADDAQIDKLFADIGA
AWGKFDGFVHSIGFAPREAIAGNFLEGLSREAFKVAHDISAYSFPAMAKAALPYLNDKSSLLTLSYLGALRSIPNYNTMG
LAKASLEASVRYLAEAVGRTEDGRCIRANGISAGPIKTLAASGIKDFGKLLGRVADAAPLRRNVTIEDVGNVAAFLLSDL
ASGVTAEITYVDGGFSQTAGLSADQV

Sequences:

>Translated_266_residues
MGFLAGKKLLITGVLSNRSIAYGIARACHQQGAELAFSYVGERFKDRITEFAAEFDSKLVFDCDVADDAQIDKLFADIGA
AWGKFDGFVHSIGFAPREAIAGNFLEGLSREAFKVAHDISAYSFPAMAKAALPYLNDKSSLLTLSYLGALRSIPNYNTMG
LAKASLEASVRYLAEAVGRTEDGRCIRANGISAGPIKTLAASGIKDFGKLLGRVADAAPLRRNVTIEDVGNVAAFLLSDL
ASGVTAEITYVDGGFSQTAGLSADQV
>Mature_265_residues
GFLAGKKLLITGVLSNRSIAYGIARACHQQGAELAFSYVGERFKDRITEFAAEFDSKLVFDCDVADDAQIDKLFADIGAA
WGKFDGFVHSIGFAPREAIAGNFLEGLSREAFKVAHDISAYSFPAMAKAALPYLNDKSSLLTLSYLGALRSIPNYNTMGL
AKASLEASVRYLAEAVGRTEDGRCIRANGISAGPIKTLAASGIKDFGKLLGRVADAAPLRRNVTIEDVGNVAAFLLSDLA
SGVTAEITYVDGGFSQTAGLSADQV

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=259, Percent_Identity=61.3899613899614, Blast_Score=327, Evalue=5e-91,
Organism=Escherichia coli, GI87082100, Length=263, Percent_Identity=28.8973384030418, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 28163; Mature: 28032

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFLAGKKLLITGVLSNRSIAYGIARACHQQGAELAFSYVGERFKDRITEFAAEFDSKLV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FDCDVADDAQIDKLFADIGAAWGKFDGFVHSIGFAPREAIAGNFLEGLSREAFKVAHDIS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYSFPAMAKAALPYLNDKSSLLTLSYLGALRSIPNYNTMGLAKASLEASVRYLAEAVGRT
HHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EDGRCIRANGISAGPIKTLAASGIKDFGKLLGRVADAAPLRRNVTIEDVGNVAAFLLSDL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHH
ASGVTAEITYVDGGFSQTAGLSADQV
HCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
GFLAGKKLLITGVLSNRSIAYGIARACHQQGAELAFSYVGERFKDRITEFAAEFDSKLV
CCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
FDCDVADDAQIDKLFADIGAAWGKFDGFVHSIGFAPREAIAGNFLEGLSREAFKVAHDIS
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AYSFPAMAKAALPYLNDKSSLLTLSYLGALRSIPNYNTMGLAKASLEASVRYLAEAVGRT
HHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
EDGRCIRANGISAGPIKTLAASGIKDFGKLLGRVADAAPLRRNVTIEDVGNVAAFLLSDL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCHHHHHHHHHHHHHHH
ASGVTAEITYVDGGFSQTAGLSADQV
HCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]