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The map label for this gene is dapE

Identifier: 222111085

GI number: 222111085

Start: 2015798

End: 2016973

Strand: Direct

Name: dapE

Synonym: Dtpsy_1892

Alternate gene names: 222111085

Gene position: 2015798-2016973 (Clockwise)

Preceding gene: 222111084

Following gene: 222111086

Centisome position: 53.1

GC content: 67.77

Gene sequence:

>1176_bases
ATGTCCCCCACCCTGCTACTGACCGAACAGCTCATCGCCCACCCCTCCGTCACGCCCGACGACGCGGGCTGCCTGGACCT
GCTGGCCCGGCGCCTCGCTCCCTTGGGCTTTGCCTGCGAACGACTGGACAGCGGGCCCGAGAACTTCCGCGTCAGCAATT
TGTGGTCAAAACGCACTGCAGCGCGCTCTGGACAAGCGCAAGCAGCTACCAAAACAGTAGTATTTGCCGGCCACACCGAC
GTGGTGCCCACGGGCCCCGTGGAGCAGTGGAGCAGCCCGCCGTTCACCCCCACACACCGGGACGGCCGCCTGTACGGCCG
CGGCGCGAGTGACATGAAGGCCTCGATCGCCGCATTCGTCGTCGCGGTCGAGGAGTTCCTGGCCGCCACGCCCGACCCGC
GCCTGGATATCGCGCTGCTGCTGACCAGCGACGAGGAAGGCCCCTCGGTGGACGGTACCAAGGTGGTCATTGAGCAGCTG
CGCGCGCGCGGGGAGCGGCTGGACTGGTGCATCGTGGGCGAACCCACCTCGGTCGAGCAGACCGGCGACATGATCAAGAA
CGGCCGCCGCGGCACGCTGTCCGGGCGGCTCACCGTGCGGGGGGTGCAGGGCCACATCGCCTACCCGCAGCTGGCGCGCA
ACCCCATCCATCAGGCCGTGCCCGCGCTCACCGAGCTCGCCGCCACCGTGTGGGATGAGGGCAACGCCTTCTTTCCGCCG
ACCAGCTGGCAGATGAGCAACATCCACGGCGGCACCGGCGCGACCAACGTGATCCCCGGCCAGGTGGTGATCGACTTCAA
CTTCCGCTTCAGCACCGAATCGACCGCCGAAGGGCTGCAGCAGCGCGTGCATGCGGTATTGGACCGCCACGGACTGGAAT
ACGACCTGACCTGGACGCTGGGCGGCCAGCCCTTCCTGACCACGCCGGGCGAACTGGTGCAGGCGGTGCAGCAAGCCATC
CGCGCCGAGACCGGTCTGGAAACCGAGCTGTCCACCACCGGCGGCACCAGCGACGGGCGCTTCATTGCACAGATTTGCCC
CCAGGTGATCGAGCTGGGCCCGCCCAACGCCAGCATCCACAAGATCGACGAGAACGTGCGCTTGGTCGACATCGAGCCGC
TCAAGAACATCTACCGCCGCACGCTCGACCACCTCAACGCACTGGCGGGCGCATGA

Upstream 100 bases:

>100_bases
TCCGAGGACGATGCCCCCTCGTTCACCGAGATCGTGCTGGACGTCAAGCCCGCCTGAGCCCGTTTCGCGGCCGCTGCACG
GCAGTGCGCCGCCTGTACCC

Downstream 100 bases:

>100_bases
CCGCGGCAGTCCAGGGCGACACCATCGGGGCGCTGGTCGCCAGCGGCGCCGCGCGCCTCACACAGGCCGGCGTGGCCTTC
GGCCACGGCACCGCCAACGC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSPTLLLTEQLIAHPSVTPDDAGCLDLLARRLAPLGFACERLDSGPENFRVSNLWSKRTAARSGQAQAATKTVVFAGHTD
VVPTGPVEQWSSPPFTPTHRDGRLYGRGASDMKASIAAFVVAVEEFLAATPDPRLDIALLLTSDEEGPSVDGTKVVIEQL
RARGERLDWCIVGEPTSVEQTGDMIKNGRRGTLSGRLTVRGVQGHIAYPQLARNPIHQAVPALTELAATVWDEGNAFFPP
TSWQMSNIHGGTGATNVIPGQVVIDFNFRFSTESTAEGLQQRVHAVLDRHGLEYDLTWTLGGQPFLTTPGELVQAVQQAI
RAETGLETELSTTGGTSDGRFIAQICPQVIELGPPNASIHKIDENVRLVDIEPLKNIYRRTLDHLNALAGA

Sequences:

>Translated_391_residues
MSPTLLLTEQLIAHPSVTPDDAGCLDLLARRLAPLGFACERLDSGPENFRVSNLWSKRTAARSGQAQAATKTVVFAGHTD
VVPTGPVEQWSSPPFTPTHRDGRLYGRGASDMKASIAAFVVAVEEFLAATPDPRLDIALLLTSDEEGPSVDGTKVVIEQL
RARGERLDWCIVGEPTSVEQTGDMIKNGRRGTLSGRLTVRGVQGHIAYPQLARNPIHQAVPALTELAATVWDEGNAFFPP
TSWQMSNIHGGTGATNVIPGQVVIDFNFRFSTESTAEGLQQRVHAVLDRHGLEYDLTWTLGGQPFLTTPGELVQAVQQAI
RAETGLETELSTTGGTSDGRFIAQICPQVIELGPPNASIHKIDENVRLVDIEPLKNIYRRTLDHLNALAGA
>Mature_390_residues
SPTLLLTEQLIAHPSVTPDDAGCLDLLARRLAPLGFACERLDSGPENFRVSNLWSKRTAARSGQAQAATKTVVFAGHTDV
VPTGPVEQWSSPPFTPTHRDGRLYGRGASDMKASIAAFVVAVEEFLAATPDPRLDIALLLTSDEEGPSVDGTKVVIEQLR
ARGERLDWCIVGEPTSVEQTGDMIKNGRRGTLSGRLTVRGVQGHIAYPQLARNPIHQAVPALTELAATVWDEGNAFFPPT
SWQMSNIHGGTGATNVIPGQVVIDFNFRFSTESTAEGLQQRVHAVLDRHGLEYDLTWTLGGQPFLTTPGELVQAVQQAIR
AETGLETELSTTGGTSDGRFIAQICPQVIELGPPNASIHKIDENVRLVDIEPLKNIYRRTLDHLNALAGA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Homo sapiens, GI21071039, Length=121, Percent_Identity=32.2314049586777, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788816, Length=386, Percent_Identity=52.8497409326425, Blast_Score=413, Evalue=1e-116,
Organism=Escherichia coli, GI1790395, Length=405, Percent_Identity=25.679012345679, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17551016, Length=281, Percent_Identity=25.2669039145907, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319758, Length=263, Percent_Identity=23.9543726235741, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_ACIET (B9MJL1)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002553349.1
- ProteinModelPortal:   B9MJL1
- MEROPS:   M20.010
- GeneID:   7382253
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1892
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 42215; Mature: 42084

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 80-80 ACT_SITE 145-145

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTLLLTEQLIAHPSVTPDDAGCLDLLARRLAPLGFACERLDSGPENFRVSNLWSKRTA
CCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCEEHHHHHHHHH
ARSGQAQAATKTVVFAGHTDVVPTGPVEQWSSPPFTPTHRDGRLYGRGASDMKASIAAFV
HCCCCCHHHEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
VAVEEFLAATPDPRLDIALLLTSDEEGPSVDGTKVVIEQLRARGERLDWCIVGEPTSVEQ
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHH
TGDMIKNGRRGTLSGRLTVRGVQGHIAYPQLARNPIHQAVPALTELAATVWDEGNAFFPP
HHHHHHCCCCCEEECEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCEECCC
TSWQMSNIHGGTGATNVIPGQVVIDFNFRFSTESTAEGLQQRVHAVLDRHGLEYDLTWTL
CCCEECCCCCCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GGQPFLTTPGELVQAVQQAIRAETGLETELSTTGGTSDGRFIAQICPQVIELGPPNASIH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEHHHHHHHHHHCCCCCCCEE
KIDENVRLVDIEPLKNIYRRTLDHLNALAGA
EECCCEEEEEHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPTLLLTEQLIAHPSVTPDDAGCLDLLARRLAPLGFACERLDSGPENFRVSNLWSKRTA
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCCCCEEHHHHHHHHH
ARSGQAQAATKTVVFAGHTDVVPTGPVEQWSSPPFTPTHRDGRLYGRGASDMKASIAAFV
HCCCCCHHHEEEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHHHH
VAVEEFLAATPDPRLDIALLLTSDEEGPSVDGTKVVIEQLRARGERLDWCIVGEPTSVEQ
HHHHHHHHCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHH
TGDMIKNGRRGTLSGRLTVRGVQGHIAYPQLARNPIHQAVPALTELAATVWDEGNAFFPP
HHHHHHCCCCCEEECEEEEEECCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCEECCC
TSWQMSNIHGGTGATNVIPGQVVIDFNFRFSTESTAEGLQQRVHAVLDRHGLEYDLTWTL
CCCEECCCCCCCCCCCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GGQPFLTTPGELVQAVQQAIRAETGLETELSTTGGTSDGRFIAQICPQVIELGPPNASIH
CCCCCCCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCEEHHHHHHHHHHCCCCCCCEE
KIDENVRLVDIEPLKNIYRRTLDHLNALAGA
EECCCEEEEEHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA