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The map label for this gene is pilT [H]

Identifier: 222111084

GI number: 222111084

Start: 2014591

End: 2015754

Strand: Direct

Name: pilT [H]

Synonym: Dtpsy_1891

Alternate gene names: 222111084

Gene position: 2014591-2015754 (Clockwise)

Preceding gene: 222111083

Following gene: 222111085

Centisome position: 53.06

GC content: 65.38

Gene sequence:

>1164_bases
ATGAGCACGATGGAGCGGATTCTGCGCCTGATGGCCGACAAGAAGGCATCCGATGTCTACCTGTCGGCCAACGCACCGGC
GCTGATCAAGATCAACGGCGAATGCGTGCCGATCAACAGCCAGATCCTGCCGCCCGACGCGCCCAAGAACCTGCTGTCCG
AGATCGTCCCCCCCGACCGCATCGAAGAGCTGGAAGAGACCGGTGAGCTCAACATGGGTGTGCCGCTCACCGGCGTAGGG
CGCTTTCGCGTCAGCGCGATGCGCCAGCGCGGCACCTATGCGGTGGTGATCCGCTTCATCACGCAGAACATCCCGCAGTT
GTCCACGCTGCACCTGCCGCCGGTGCTCAGCGAGCTGATCATGGAAAAGCGCGGCCTGATCCTGCTCGTGGGCGCCACGG
GCTCGGGCAAGAGCACGACGCTGGCGGCGATGCTGGACGCGCGCAACGAGTCCATGACCGGCCACATCCTCACGGTGGAG
GACCCGGTCGAATACCAGTTCAAGAACAAGAAGTCCATCGTCAACCAGCGGGAGATCGGCAGCGACACCCAGTCGCTGCA
GACCGCGCTCAAGAACGCGCTGCGACAGGCGCCCGACGTGATCCTGATCGGCGAAATCCGTGACCGCGAGACCATGTCCG
CCGCCATCGCCTACGCCCAGTCGGGCCACCTGTGCCTGGCCACGCTGCACGGCAACAACAGCTACCACGCCCTCAACCGC
ATCCTGTCGTTCTACCCCGTGGAGGTGCGCCCCACGATGCTGGGCGACCTGGCGTCCGCGCTCAAGGCCATCATCTCGCA
GCGCCTGGTGCGCACCACGACCGGCGAGCGCGTGCCCGCGGCGGAGGTCATGCTCAACACCAAGCTGGTGGCCGAGCTGG
TGGAAAAGGGCGACTTCTCGGGGATCCGCGAGGCCATGGAAAAATCCATGGCCGAAGGCTCGCAGACCTTCGAGGAAGCG
CTGGCACACCTGATCCTGGAGGGCAAGATCGACCGCAAGGAAGGCCTGGCCTACGCCGACTCCCCCACCAACCTTATGTG
GCGCCTGCAAAACGACTTCTCGGCTGCCGCGAAGTCGGCGCGCGCCGTCAACGATGCCGGCCAGCAGTCCGAGGACGATG
CCCCCTCGTTCACCGAGATCGTGCTGGACGTCAAGCCCGCCTGA

Upstream 100 bases:

>100_bases
AGACCCGCTCCACCACCAGCCTGAACGACCTGCTGCGCGACTGAAGCTACGCCGCACCGGCACTGCACGCATACATAACC
AGCACTGAGAGGGAACCAGC

Downstream 100 bases:

>100_bases
GCCCGTTTCGCGGCCGCTGCACGGCAGTGCGCCGCCTGTACCCATGTCCCCCACCCTGCTACTGACCGAACAGCTCATCG
CCCACCCCTCCGTCACGCCC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MSTMERILRLMADKKASDVYLSANAPALIKINGECVPINSQILPPDAPKNLLSEIVPPDRIEELEETGELNMGVPLTGVG
RFRVSAMRQRGTYAVVIRFITQNIPQLSTLHLPPVLSELIMEKRGLILLVGATGSGKSTTLAAMLDARNESMTGHILTVE
DPVEYQFKNKKSIVNQREIGSDTQSLQTALKNALRQAPDVILIGEIRDRETMSAAIAYAQSGHLCLATLHGNNSYHALNR
ILSFYPVEVRPTMLGDLASALKAIISQRLVRTTTGERVPAAEVMLNTKLVAELVEKGDFSGIREAMEKSMAEGSQTFEEA
LAHLILEGKIDRKEGLAYADSPTNLMWRLQNDFSAAAKSARAVNDAGQQSEDDAPSFTEIVLDVKPA

Sequences:

>Translated_387_residues
MSTMERILRLMADKKASDVYLSANAPALIKINGECVPINSQILPPDAPKNLLSEIVPPDRIEELEETGELNMGVPLTGVG
RFRVSAMRQRGTYAVVIRFITQNIPQLSTLHLPPVLSELIMEKRGLILLVGATGSGKSTTLAAMLDARNESMTGHILTVE
DPVEYQFKNKKSIVNQREIGSDTQSLQTALKNALRQAPDVILIGEIRDRETMSAAIAYAQSGHLCLATLHGNNSYHALNR
ILSFYPVEVRPTMLGDLASALKAIISQRLVRTTTGERVPAAEVMLNTKLVAELVEKGDFSGIREAMEKSMAEGSQTFEEA
LAHLILEGKIDRKEGLAYADSPTNLMWRLQNDFSAAAKSARAVNDAGQQSEDDAPSFTEIVLDVKPA
>Mature_386_residues
STMERILRLMADKKASDVYLSANAPALIKINGECVPINSQILPPDAPKNLLSEIVPPDRIEELEETGELNMGVPLTGVGR
FRVSAMRQRGTYAVVIRFITQNIPQLSTLHLPPVLSELIMEKRGLILLVGATGSGKSTTLAAMLDARNESMTGHILTVED
PVEYQFKNKKSIVNQREIGSDTQSLQTALKNALRQAPDVILIGEIRDRETMSAAIAYAQSGHLCLATLHGNNSYHALNRI
LSFYPVEVRPTMLGDLASALKAIISQRLVRTTTGERVPAAEVMLNTKLVAELVEKGDFSGIREAMEKSMAEGSQTFEEAL
AHLILEGKIDRKEGLAYADSPTNLMWRLQNDFSAAAKSARAVNDAGQQSEDDAPSFTEIVLDVKPA

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=329, Percent_Identity=37.6899696048632, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786296, Length=282, Percent_Identity=32.9787234042553, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789723, Length=183, Percent_Identity=42.0765027322404, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 42232; Mature: 42101

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTMERILRLMADKKASDVYLSANAPALIKINGECVPINSQILPPDAPKNLLSEIVPPDR
CCHHHHHHHHHHCCCCCCEEEECCCCEEEEECCEEEECCCCCCCCCCCHHHHHHCCCCHH
IEELEETGELNMGVPLTGVGRFRVSAMRQRGTYAVVIRFITQNIPQLSTLHLPPVLSELI
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCHHHHHHH
MEKRGLILLVGATGSGKSTTLAAMLDARNESMTGHILTVEDPVEYQFKNKKSIVNQREIG
HHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCC
SDTQSLQTALKNALRQAPDVILIGEIRDRETMSAAIAYAQSGHLCLATLHGNNSYHALNR
CHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
ILSFYPVEVRPTMLGDLASALKAIISQRLVRTTTGERVPAAEVMLNTKLVAELVEKGDFS
HHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHH
GIREAMEKSMAEGSQTFEEALAHLILEGKIDRKEGLAYADSPTNLMWRLQNDFSAAAKSA
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHCCEECCCCCCEEEEECCHHHHHHHHH
RAVNDAGQQSEDDAPSFTEIVLDVKPA
HHHHHCCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure 
STMERILRLMADKKASDVYLSANAPALIKINGECVPINSQILPPDAPKNLLSEIVPPDR
CHHHHHHHHHHCCCCCCEEEECCCCEEEEECCEEEECCCCCCCCCCCHHHHHHCCCCHH
IEELEETGELNMGVPLTGVGRFRVSAMRQRGTYAVVIRFITQNIPQLSTLHLPPVLSELI
HHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEECCCHHHHHHH
MEKRGLILLVGATGSGKSTTLAAMLDARNESMTGHILTVEDPVEYQFKNKKSIVNQREIG
HHCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCC
SDTQSLQTALKNALRQAPDVILIGEIRDRETMSAAIAYAQSGHLCLATLHGNNSYHALNR
CHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHH
ILSFYPVEVRPTMLGDLASALKAIISQRLVRTTTGERVPAAEVMLNTKLVAELVEKGDFS
HHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHH
GIREAMEKSMAEGSQTFEEALAHLILEGKIDRKEGLAYADSPTNLMWRLQNDFSAAAKSA
HHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHCCEECCCCCCEEEEECCHHHHHHHHH
RAVNDAGQQSEDDAPSFTEIVLDVKPA
HHHHHCCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]