Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 222110972

GI number: 222110972

Start: 1894714

End: 1897338

Strand: Direct

Name: alaS [H]

Synonym: Dtpsy_1779

Alternate gene names: 222110972

Gene position: 1894714-1897338 (Clockwise)

Preceding gene: 222110970

Following gene: 222110978

Centisome position: 49.91

GC content: 66.17

Gene sequence:

>2625_bases
ATGAGCACACCCACTTTCTCCGTTGCAGACATTCGCAAGACCTTCCTGGACTTCTTCGCCGCCAAGGGGCATACCGTGGT
GGCGTCCAGTCCGCTGGTGCCGGGCAACGACCCCACGCTGATGTTCACCAACTCGGGCATGGTGCAGTTCAAGGATGTGT
TCCTGGGTACGGACAAGCGCCCCTATGTGCGCGCCACATCGGTGCAGACCTGCCTGCGCGCCGGCGGCAAGCACAACGAT
CTGGAGAACGTGGGCTACACCGCGCGCCACCACACGTTTTTCGAGATGCTGGGCAACTGGAGCTTTGGCGACTACTTCAA
GCGCGAGTCGCTCAAGTGGGCCTGGGAGCTGCTGACCGAGGTCTACAAGCTGCCTCCCGAGCGCTTGCTGGCCACGGTCT
ACGCCGAGGACGACGAGGCCTACGACATCTGGACCAAAGAAATCGGCCTGCCGCCCGAGCGTGTGATCCGCATCGGCGAC
AACAAGGGCGGCCGCTACAAGAGCGACAACTTCTGGATGATGGCCGACACCGGCCCCTGTGGCCCGTGCTCGGAAATCTT
CTACGACCACGGCGAGCACATTGCCGGCGGCCCCCCGGGCAGCCCGGATGAGGACGGCGACCGCTTCATCGAAATCTGGA
ACAACGTGTTCATGCAGTTCGACATGGACGAGCAAGGCAACGTCAAGCCGCTGCCCGCACCCTGCGTGGACACCGGCATG
GGCCTGGAGCGCCTGGCCGCCATCCTGCAGCACGTGCACAGCAACTACGAGATCGACCTGTTCGACGCGCTGATCAAGGC
CGCGGCGCGCGAAACAGGCACAAGCGACCTGACCAACCCGTCGCTCAAGGTCATCGCCGACCACATCCGCGCCACCGCCT
TCCTGGTCGCCGACGGTGTCATCCCCAGCAACGAGGGCCGCGGCTACGTGCAGCGCCGCATCGTGCGCCGCGCCATTCGC
CACGGCTACAAGCTGGGTCGCAAGACGCCGTTCTTCCACAAGCTGGTGCAAGACCTGGTGCAGCAGATGGGCGATGCGTA
CCCCAAGATCCGCGAGCAACAGGCGCGCATCACGGATGTGCTGCGGGTGGAAGAGGAACGCTTCTTCGAGACCCTGGCGC
ACGGCATGGAGATTCTGGACAGCGCTTTGGCTGGCGGTGCCAAGACGCTGCCGGGCGACGTGGCGTTCAAGCTGCACGAC
ACCTATGGCTTCCCGCTGGACCTGACGAACGACGTGTGCCGCGAACGGGGCGTCAATGTGGACGAAGCGGGCTTTGCCAC
CGCCATGGAGCACCAGAAGAGCACTGCCCGCGCCGCAGGCAAGTTCAAGATGGACCGCGCGCTGGAATACACCGGCGCGG
CCAACCAGTTCACGGGCTACGAGCAACTGGCCGAAAGTGCCAAGATCGTCGCGCTGTACGTGGACGGCACCAGCACTGCG
GCCCTGCACGCGGGTCAGAGCGGCGTGGTGGTGCTGGACCGCACGCCGTTCTATGCCGAAAGCGGCGGCCAGGTGGGCGA
CCAAGGCACCATCGGCGCTGGCTCGGCGTGCTTCAGCGTGGCCGACACGCAGAAAATCAAGGCCGACGTCTACGGCCACC
ACGGCACGCTGGAAGCCGGCACGCTGAATGTGGGCGACACGGTGCAGGCGCAGGTGGACCTGCAACTGCGCGCGGCCACC
ATGCGCAACCACTCGGTCACCCACCTCATGCACAAGGCGTTGCGCGAGGTGCTGGGCGACCATGTGCAGCAAAAGGGCAG
CCTGGTGAACGCCGAGCGCACGCGCTTTGACTTCGCGCACAACGCGCCGCTGACGGCCGCGCAGATTCGCGAGATCGAAC
GCCTGGTGAATGCCGAGGTGCTGGCCAATACCGACACTAACGCGCGCCTGATGGATATCGAAAGCGCGCAGAAAACGGGC
GCCATGATGTTGTTCGGCGAGAAGTACGGCGAGACTGTGCGTGTGCTGGACATCGGCACCAGCCGCGAGCTGTGCGGCGG
CACGCACGTGCGCCGCACGGGCGACATCGGCCTGTTCAAGGTGGTGGCTGAGGGCGGCGTGGCCGCAGGCGTGCGCCGCA
TCGAGGCGGTGACCGGCGAGAACGCGCTGGCCTACCTGCAGTCGCTGGAGTCCACGGTGGACCAGGCTGCAGCCGCCCTG
AAGGCGCCCCCCGCGGAGCTGACCGCCCGCATTGGCGGTGCGCTCGATCAGATCAAGACGCTCGAGAAGGAACTGGCTGC
ACTCAAGGGCAAGCTGGCTTCCAGCCAGGGCGATGAGCTGGCCGGCCAGGCCGTGGACGTCAAGGGCATCAAGGTGCTGG
CGGCCCGGCTGGAAGGCGCGGACGCCAAGACCCTGCGCGAGACCATGGACAAGCTCAAGGACAAGCTCAAGACGGCGGCC
ATCGTGCTGGCGGCCGTCGATGGCGACAAGGTGCAGCTGGCGGCTGGCGTGACGGCCGACAGCATCGGCCGTGTCAAGGC
CGGGGATCTGGTCAACTTCGTGGCGGCCCAGGTGGGTGGCAAAGGGGGCGGAAAACCCGACATGGCTATGGCCGGCGGCA
CCAACGCGGCGGCGCTGCCGCAGGCGCTGGCAGCCGTGCAGGGCTGGGTGGGCGAGCGCATCTGA

Upstream 100 bases:

>100_bases
GATAGCGCCGTGTGCACGCAAGGGTGAAATCTATAATGGCAGGTTGGTCACGACGGGATGCCTTGCGCTGCCATACCCGC
CCACGAACCCCATACACACC

Downstream 100 bases:

>100_bases
CGACGGCCTGGGCCCCCCAGACGGGGGCCCGGTGCACGTGCGGACATGGTTGTACCCGCGCGGGCGGCGGCGGCCTGCGC
CGCGGTTACACCGGACCCAG

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 874; Mature: 873

Protein sequence:

>874_residues
MSTPTFSVADIRKTFLDFFAAKGHTVVASSPLVPGNDPTLMFTNSGMVQFKDVFLGTDKRPYVRATSVQTCLRAGGKHND
LENVGYTARHHTFFEMLGNWSFGDYFKRESLKWAWELLTEVYKLPPERLLATVYAEDDEAYDIWTKEIGLPPERVIRIGD
NKGGRYKSDNFWMMADTGPCGPCSEIFYDHGEHIAGGPPGSPDEDGDRFIEIWNNVFMQFDMDEQGNVKPLPAPCVDTGM
GLERLAAILQHVHSNYEIDLFDALIKAAARETGTSDLTNPSLKVIADHIRATAFLVADGVIPSNEGRGYVQRRIVRRAIR
HGYKLGRKTPFFHKLVQDLVQQMGDAYPKIREQQARITDVLRVEEERFFETLAHGMEILDSALAGGAKTLPGDVAFKLHD
TYGFPLDLTNDVCRERGVNVDEAGFATAMEHQKSTARAAGKFKMDRALEYTGAANQFTGYEQLAESAKIVALYVDGTSTA
ALHAGQSGVVVLDRTPFYAESGGQVGDQGTIGAGSACFSVADTQKIKADVYGHHGTLEAGTLNVGDTVQAQVDLQLRAAT
MRNHSVTHLMHKALREVLGDHVQQKGSLVNAERTRFDFAHNAPLTAAQIREIERLVNAEVLANTDTNARLMDIESAQKTG
AMMLFGEKYGETVRVLDIGTSRELCGGTHVRRTGDIGLFKVVAEGGVAAGVRRIEAVTGENALAYLQSLESTVDQAAAAL
KAPPAELTARIGGALDQIKTLEKELAALKGKLASSQGDELAGQAVDVKGIKVLAARLEGADAKTLRETMDKLKDKLKTAA
IVLAAVDGDKVQLAAGVTADSIGRVKAGDLVNFVAAQVGGKGGGKPDMAMAGGTNAAALPQALAAVQGWVGERI

Sequences:

>Translated_874_residues
MSTPTFSVADIRKTFLDFFAAKGHTVVASSPLVPGNDPTLMFTNSGMVQFKDVFLGTDKRPYVRATSVQTCLRAGGKHND
LENVGYTARHHTFFEMLGNWSFGDYFKRESLKWAWELLTEVYKLPPERLLATVYAEDDEAYDIWTKEIGLPPERVIRIGD
NKGGRYKSDNFWMMADTGPCGPCSEIFYDHGEHIAGGPPGSPDEDGDRFIEIWNNVFMQFDMDEQGNVKPLPAPCVDTGM
GLERLAAILQHVHSNYEIDLFDALIKAAARETGTSDLTNPSLKVIADHIRATAFLVADGVIPSNEGRGYVQRRIVRRAIR
HGYKLGRKTPFFHKLVQDLVQQMGDAYPKIREQQARITDVLRVEEERFFETLAHGMEILDSALAGGAKTLPGDVAFKLHD
TYGFPLDLTNDVCRERGVNVDEAGFATAMEHQKSTARAAGKFKMDRALEYTGAANQFTGYEQLAESAKIVALYVDGTSTA
ALHAGQSGVVVLDRTPFYAESGGQVGDQGTIGAGSACFSVADTQKIKADVYGHHGTLEAGTLNVGDTVQAQVDLQLRAAT
MRNHSVTHLMHKALREVLGDHVQQKGSLVNAERTRFDFAHNAPLTAAQIREIERLVNAEVLANTDTNARLMDIESAQKTG
AMMLFGEKYGETVRVLDIGTSRELCGGTHVRRTGDIGLFKVVAEGGVAAGVRRIEAVTGENALAYLQSLESTVDQAAAAL
KAPPAELTARIGGALDQIKTLEKELAALKGKLASSQGDELAGQAVDVKGIKVLAARLEGADAKTLRETMDKLKDKLKTAA
IVLAAVDGDKVQLAAGVTADSIGRVKAGDLVNFVAAQVGGKGGGKPDMAMAGGTNAAALPQALAAVQGWVGERI
>Mature_873_residues
STPTFSVADIRKTFLDFFAAKGHTVVASSPLVPGNDPTLMFTNSGMVQFKDVFLGTDKRPYVRATSVQTCLRAGGKHNDL
ENVGYTARHHTFFEMLGNWSFGDYFKRESLKWAWELLTEVYKLPPERLLATVYAEDDEAYDIWTKEIGLPPERVIRIGDN
KGGRYKSDNFWMMADTGPCGPCSEIFYDHGEHIAGGPPGSPDEDGDRFIEIWNNVFMQFDMDEQGNVKPLPAPCVDTGMG
LERLAAILQHVHSNYEIDLFDALIKAAARETGTSDLTNPSLKVIADHIRATAFLVADGVIPSNEGRGYVQRRIVRRAIRH
GYKLGRKTPFFHKLVQDLVQQMGDAYPKIREQQARITDVLRVEEERFFETLAHGMEILDSALAGGAKTLPGDVAFKLHDT
YGFPLDLTNDVCRERGVNVDEAGFATAMEHQKSTARAAGKFKMDRALEYTGAANQFTGYEQLAESAKIVALYVDGTSTAA
LHAGQSGVVVLDRTPFYAESGGQVGDQGTIGAGSACFSVADTQKIKADVYGHHGTLEAGTLNVGDTVQAQVDLQLRAATM
RNHSVTHLMHKALREVLGDHVQQKGSLVNAERTRFDFAHNAPLTAAQIREIERLVNAEVLANTDTNARLMDIESAQKTGA
MMLFGEKYGETVRVLDIGTSRELCGGTHVRRTGDIGLFKVVAEGGVAAGVRRIEAVTGENALAYLQSLESTVDQAAAALK
APPAELTARIGGALDQIKTLEKELAALKGKLASSQGDELAGQAVDVKGIKVLAARLEGADAKTLRETMDKLKDKLKTAAI
VLAAVDGDKVQLAAGVTADSIGRVKAGDLVNFVAAQVGGKGGGKPDMAMAGGTNAAALPQALAAVQGWVGERI

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=964, Percent_Identity=36.7219917012448, Blast_Score=517, Evalue=1e-146,
Organism=Homo sapiens, GI38569417, Length=761, Percent_Identity=37.0565045992116, Blast_Score=447, Evalue=1e-125,
Organism=Escherichia coli, GI1789048, Length=873, Percent_Identity=59.3356242840779, Blast_Score=1040, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=983, Percent_Identity=34.8931841302136, Blast_Score=525, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI17536681, Length=744, Percent_Identity=32.1236559139785, Blast_Score=359, Evalue=4e-99,
Organism=Saccharomyces cerevisiae, GI6324911, Length=894, Percent_Identity=36.5771812080537, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582809, Length=958, Percent_Identity=36.2212943632568, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI45552267, Length=958, Percent_Identity=36.2212943632568, Blast_Score=550, Evalue=1e-156,
Organism=Drosophila melanogaster, GI24658214, Length=780, Percent_Identity=31.2820512820513, Blast_Score=333, Evalue=4e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94379; Mature: 94248

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTFSVADIRKTFLDFFAAKGHTVVASSPLVPGNDPTLMFTNSGMVQFKDVFLGTDKR
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEEHEEECCCCC
PYVRATSVQTCLRAGGKHNDLENVGYTARHHTFFEMLGNWSFGDYFKRESLKWAWELLTE
CCEEHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VYKLPPERLLATVYAEDDEAYDIWTKEIGLPPERVIRIGDNKGGRYKSDNFWMMADTGPC
HHHCCHHHHEEEEEECCCCCHHHHHHHCCCCHHHEEEECCCCCCCEECCCEEEEECCCCC
GPCSEIFYDHGEHIAGGPPGSPDEDGDRFIEIWNNVFMQFDMDEQGNVKPLPAPCVDTGM
CCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHCCC
GLERLAAILQHVHSNYEIDLFDALIKAAARETGTSDLTNPSLKVIADHIRATAFLVADGV
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
IPSNEGRGYVQRRIVRRAIRHGYKLGRKTPFFHKLVQDLVQQMGDAYPKIREQQARITDV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LRVEEERFFETLAHGMEILDSALAGGAKTLPGDVAFKLHDTYGFPLDLTNDVCRERGVNV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCC
DEAGFATAMEHQKSTARAAGKFKMDRALEYTGAANQFTGYEQLAESAKIVALYVDGTSTA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCCCE
ALHAGQSGVVVLDRTPFYAESGGQVGDQGTIGAGSACFSVADTQKIKADVYGHHGTLEAG
EEECCCCCEEEEECCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECC
TLNVGDTVQAQVDLQLRAATMRNHSVTHLMHKALREVLGDHVQQKGSLVNAERTRFDFAH
EECCCCCEEEEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
NAPLTAAQIREIERLVNAEVLANTDTNARLMDIESAQKTGAMMLFGEKYGETVRVLDIGT
CCCCCHHHHHHHHHHHCCHHEECCCCCCEEEECCHHHHCCEEEEEECCCCCEEEEEECCC
SRELCGGTHVRRTGDIGLFKVVAEGGVAAGVRRIEAVTGENALAYLQSLESTVDQAAAAL
CCHHHCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KAPPAELTARIGGALDQIKTLEKELAALKGKLASSQGDELAGQAVDVKGIKVLAARLEGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECCCHHHHHHHHHCCC
DAKTLRETMDKLKDKLKTAAIVLAAVDGDKVQLAAGVTADSIGRVKAGDLVNFVAAQVGG
CHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHCC
KGGGKPDMAMAGGTNAAALPQALAAVQGWVGERI
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STPTFSVADIRKTFLDFFAAKGHTVVASSPLVPGNDPTLMFTNSGMVQFKDVFLGTDKR
CCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCCEEEEHEEECCCCC
PYVRATSVQTCLRAGGKHNDLENVGYTARHHTFFEMLGNWSFGDYFKRESLKWAWELLTE
CCEEHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
VYKLPPERLLATVYAEDDEAYDIWTKEIGLPPERVIRIGDNKGGRYKSDNFWMMADTGPC
HHHCCHHHHEEEEEECCCCCHHHHHHHCCCCHHHEEEECCCCCCCEECCCEEEEECCCCC
GPCSEIFYDHGEHIAGGPPGSPDEDGDRFIEIWNNVFMQFDMDEQGNVKPLPAPCVDTGM
CCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCCCHHHCCC
GLERLAAILQHVHSNYEIDLFDALIKAAARETGTSDLTNPSLKVIADHIRATAFLVADGV
CHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEECCC
IPSNEGRGYVQRRIVRRAIRHGYKLGRKTPFFHKLVQDLVQQMGDAYPKIREQQARITDV
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LRVEEERFFETLAHGMEILDSALAGGAKTLPGDVAFKLHDTYGFPLDLTNDVCRERGVNV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCCCC
DEAGFATAMEHQKSTARAAGKFKMDRALEYTGAANQFTGYEQLAESAKIVALYVDGTSTA
CCHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHHCCCEEEEEEECCCCCE
ALHAGQSGVVVLDRTPFYAESGGQVGDQGTIGAGSACFSVADTQKIKADVYGHHGTLEAG
EEECCCCCEEEEECCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEECC
TLNVGDTVQAQVDLQLRAATMRNHSVTHLMHKALREVLGDHVQQKGSLVNAERTRFDFAH
EECCCCCEEEEEEEEEEEHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCC
NAPLTAAQIREIERLVNAEVLANTDTNARLMDIESAQKTGAMMLFGEKYGETVRVLDIGT
CCCCCHHHHHHHHHHHCCHHEECCCCCCEEEECCHHHHCCEEEEEECCCCCEEEEEECCC
SRELCGGTHVRRTGDIGLFKVVAEGGVAAGVRRIEAVTGENALAYLQSLESTVDQAAAAL
CCHHHCCCCEEECCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
KAPPAELTARIGGALDQIKTLEKELAALKGKLASSQGDELAGQAVDVKGIKVLAARLEGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECCCHHHHHHHHHCCC
DAKTLRETMDKLKDKLKTAAIVLAAVDGDKVQLAAGVTADSIGRVKAGDLVNFVAAQVGG
CHHHHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCHHHCCCCCHHHHHHHHHHHHCC
KGGGKPDMAMAGGTNAAALPQALAAVQGWVGERI
CCCCCCCCEECCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA