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The map label for this gene is iscA [H]

Identifier: 222110834

GI number: 222110834

Start: 1732002

End: 1732325

Strand: Direct

Name: iscA [H]

Synonym: Dtpsy_1640

Alternate gene names: 222110834

Gene position: 1732002-1732325 (Clockwise)

Preceding gene: 222110833

Following gene: 222110835

Centisome position: 45.62

GC content: 58.95

Gene sequence:

>324_bases
ATGGCGATCACGCTGACCGAAGCTGCCGCGCGCCATGTGACGCGCTACCTCGCCCGGCGGGGAAAGGGCGTGGGCGTGCG
CCTAGGTGTCAAGACCACCGGGTGCTCAGGCCTGGCTTACAAGCTTGAATATGTCGATGAGCAGGCACCCGAAGACGTTG
TGTTTGAAAATCACGGCGTCAAGGTGCTGATCGATCCCAAGAGCCTTGCTTATATCGACGGGACCGAGTTGGATTTTGTG
CGCGAGGGCCTCAATGAAGGCTTTCGCTTCAATAACCCGAATGAGCGCGACCGCTGTGGTTGTGGAGAGAGCTTCCGCGT
GTGA

Upstream 100 bases:

>100_bases
TGAAGATCCACTGCTCCATCCTGGCGGAAGACGCCATCAAGGCCGCGGTCAACGATTACAAGGCCAAGCACGGAGCGGCT
GTCGAGGCCGCCTGAAGACC

Downstream 100 bases:

>100_bases
GCACGCCGTAGGCCGCTTATTGCACAGGACCGCCATCGCCCGCCGCGCTGGCGGTTTTTTTATTGCCATGAACCTCCAAT
CCGACGATTTCGAACTTTTT

Product: iron-sulfur cluster assembly protein isca

Products: NA

Alternate protein names: Iron-sulfur cluster assembly protein [H]

Number of amino acids: Translated: 107; Mature: 106

Protein sequence:

>107_residues
MAITLTEAAARHVTRYLARRGKGVGVRLGVKTTGCSGLAYKLEYVDEQAPEDVVFENHGVKVLIDPKSLAYIDGTELDFV
REGLNEGFRFNNPNERDRCGCGESFRV

Sequences:

>Translated_107_residues
MAITLTEAAARHVTRYLARRGKGVGVRLGVKTTGCSGLAYKLEYVDEQAPEDVVFENHGVKVLIDPKSLAYIDGTELDFV
REGLNEGFRFNNPNERDRCGCGESFRV
>Mature_106_residues
AITLTEAAARHVTRYLARRGKGVGVRLGVKTTGCSGLAYKLEYVDEQAPEDVVFENHGVKVLIDPKSLAYIDGTELDFVR
EGLNEGFRFNNPNERDRCGCGESFRV

Specific function: Is able to transfer iron-sulfur clusters to apo- ferredoxin. Multiple cycles of [2Fe2S] cluster formation and transfer are observed, suggesting that iscA acts catalytically. Recruits intracellular free iron so as to provide iron for the assembly of transi

COG id: COG0316

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesB/iscA family [H]

Homologues:

Organism=Homo sapiens, GI52426768, Length=106, Percent_Identity=42.4528301886792, Blast_Score=95, Evalue=1e-20,
Organism=Homo sapiens, GI160420328, Length=83, Percent_Identity=39.7590361445783, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1788877, Length=107, Percent_Identity=66.3551401869159, Blast_Score=160, Evalue=2e-41,
Organism=Escherichia coli, GI1787974, Length=105, Percent_Identity=51.4285714285714, Blast_Score=119, Evalue=4e-29,
Organism=Escherichia coli, GI1786351, Length=110, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25146411, Length=106, Percent_Identity=39.622641509434, Blast_Score=91, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI193209025, Length=106, Percent_Identity=39.622641509434, Blast_Score=91, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323001, Length=105, Percent_Identity=42.8571428571429, Blast_Score=92, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24642263, Length=106, Percent_Identity=43.3962264150943, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000361
- InterPro:   IPR016092
- InterPro:   IPR017870
- InterPro:   IPR011302 [H]

Pfam domain/function: PF01521 Fe-S_biosyn [H]

EC number: NA

Molecular weight: Translated: 11866; Mature: 11734

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01152 HESB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITLTEAAARHVTRYLARRGKGVGVRLGVKTTGCSGLAYKLEYVDEQAPEDVVFENHGV
CEEEEHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCEEEEEEECCCCCCCCEEECCCCC
KVLIDPKSLAYIDGTELDFVREGLNEGFRFNNPNERDRCGCGESFRV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AITLTEAAARHVTRYLARRGKGVGVRLGVKTTGCSGLAYKLEYVDEQAPEDVVFENHGV
EEEEHHHHHHHHHHHHHHCCCCCEEEEECEECCCCCEEEEEEECCCCCCCCEEECCCCC
KVLIDPKSLAYIDGTELDFVREGLNEGFRFNNPNERDRCGCGESFRV
EEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA