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The map label for this gene is rlmB [H]

Identifier: 222110801

GI number: 222110801

Start: 1699567

End: 1700313

Strand: Direct

Name: rlmB [H]

Synonym: Dtpsy_1606

Alternate gene names: 222110801

Gene position: 1699567-1700313 (Clockwise)

Preceding gene: 222110799

Following gene: 222110806

Centisome position: 44.77

GC content: 70.01

Gene sequence:

>747_bases
ATGTCCTCCCCCAAAGTCCTCTTCGGCTTTCACGCCGTGGGCGTGCGCCTGAAGACCGCGCCGCAAAGCATCATCGAGAT
CTATTACGAGCCCACGCGCCGCGACGCGCGCATGCGCCAGTTCCTGGAGCGCGCACGCGAGGCGGGGGCGCGCCTGATCG
AAGCCGACGGCCTGCGCCTGGCCAAGCTGGCCGGCAGCCATGGCCACCAGGGCGTGGCGGCGCGGGTCGAGGCCGTGGCG
CAGGTGCGCACGCTGGACGAGCTGCTGGACGAGCTGGAGGCCCGTGGCGTGAAAAATCCGCTGCTGCTGGTGCTGGACGG
CGTGACCGACCCGCACAACCTGGGCGCCTGCTTGCGCGTGGCCGATGGCGCGGGCGCGCATGCGGTGATCGCCCCCAAGG
ACCATGCGGTGGGCATCAACGCCACCGTGGCCAAGGTGGCCAGCGGCGCGGCCGAGACGGTGCCGTACTTCATGGTCACG
AACCTGGCGCGCACGCTGAACGAGCTCAAGGAGCGCAACATCTGGGTCATCGGCACCAGTGGCGATGCCGACAAGACGCT
GTACCAGGTGGACCTGAAAGGCCCGGTCGCCCTGGTGCTGGGCGCCGAGGGCCCGGGCATGCGCCAACTCACGCGCAAGA
CCTGCGACGAACTGGTGGGCATCCCCATGCAGGGCGCTGTCGAGAGCCTGAACGTGTCGGTGGCCAGCGCGGTGTGCCTC
TACGAGGCGCTGCGCCAGCGCGCCTGA

Upstream 100 bases:

>100_bases
CGTTTGTCAAGGGAAGAAACGCGGATTTTAGCGGCTGTCCGGCGGTGGCCTGCGCCGCGCCACGGTGCCAGTGGGGTCGC
CGGCGGGATAATCGCTACCC

Downstream 100 bases:

>100_bases
ACGCCGAAGGCGCCCGGCCCCTAGCCGCGGTGCGCCGCCTCGATGGCGGCAATGTCGATCTTGCGCATCTGCATCATCGC
GTCGAACGCGCGCTTGGCGG

Product: TrmH family RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSSPKVLFGFHAVGVRLKTAPQSIIEIYYEPTRRDARMRQFLERAREAGARLIEADGLRLAKLAGSHGHQGVAARVEAVA
QVRTLDELLDELEARGVKNPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETVPYFMVT
NLARTLNELKERNIWVIGTSGDADKTLYQVDLKGPVALVLGAEGPGMRQLTRKTCDELVGIPMQGAVESLNVSVASAVCL
YEALRQRA

Sequences:

>Translated_248_residues
MSSPKVLFGFHAVGVRLKTAPQSIIEIYYEPTRRDARMRQFLERAREAGARLIEADGLRLAKLAGSHGHQGVAARVEAVA
QVRTLDELLDELEARGVKNPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETVPYFMVT
NLARTLNELKERNIWVIGTSGDADKTLYQVDLKGPVALVLGAEGPGMRQLTRKTCDELVGIPMQGAVESLNVSVASAVCL
YEALRQRA
>Mature_247_residues
SSPKVLFGFHAVGVRLKTAPQSIIEIYYEPTRRDARMRQFLERAREAGARLIEADGLRLAKLAGSHGHQGVAARVEAVAQ
VRTLDELLDELEARGVKNPLLLVLDGVTDPHNLGACLRVADGAGAHAVIAPKDHAVGINATVAKVASGAAETVPYFMVTN
LARTLNELKERNIWVIGTSGDADKTLYQVDLKGPVALVLGAEGPGMRQLTRKTCDELVGIPMQGAVESLNVSVASAVCLY
EALRQRA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=162, Percent_Identity=32.7160493827161, Blast_Score=75, Evalue=7e-14,
Organism=Escherichia coli, GI1790623, Length=244, Percent_Identity=50.8196721311475, Blast_Score=240, Evalue=7e-65,
Organism=Escherichia coli, GI1790083, Length=152, Percent_Identity=35.5263157894737, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788935, Length=242, Percent_Identity=26.8595041322314, Blast_Score=75, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=167, Percent_Identity=34.1317365269461, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26506; Mature: 26375

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPKVLFGFHAVGVRLKTAPQSIIEIYYEPTRRDARMRQFLERAREAGARLIEADGLRL
CCCCCEEEEEHEECEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCEE
AKLAGSHGHQGVAARVEAVAQVRTLDELLDELEARGVKNPLLLVLDGVTDPHNLGACLRV
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEE
ADGAGAHAVIAPKDHAVGINATVAKVASGAAETVPYFMVTNLARTLNELKERNIWVIGTS
CCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
GDADKTLYQVDLKGPVALVLGAEGPGMRQLTRKTCDELVGIPMQGAVESLNVSVASAVCL
CCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YEALRQRA
HHHHHHCC
>Mature Secondary Structure 
SSPKVLFGFHAVGVRLKTAPQSIIEIYYEPTRRDARMRQFLERAREAGARLIEADGLRL
CCCCEEEEEHEECEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCEE
AKLAGSHGHQGVAARVEAVAQVRTLDELLDELEARGVKNPLLLVLDGVTDPHNLGACLRV
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEE
ADGAGAHAVIAPKDHAVGINATVAKVASGAAETVPYFMVTNLARTLNELKERNIWVIGTS
CCCCCCEEEECCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECC
GDADKTLYQVDLKGPVALVLGAEGPGMRQLTRKTCDELVGIPMQGAVESLNVSVASAVCL
CCCCCEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
YEALRQRA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]