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The map label for this gene is vraB [H]

Identifier: 222110739

GI number: 222110739

Start: 1624760

End: 1625929

Strand: Direct

Name: vraB [H]

Synonym: Dtpsy_1542

Alternate gene names: 222110739

Gene position: 1624760-1625929 (Clockwise)

Preceding gene: 222110738

Following gene: 222110740

Centisome position: 42.8

GC content: 75.13

Gene sequence:

>1170_bases
GTGAACGCTATTCCCATATTGGCCTGGGCGCGCACGCCGGTGGCGCCGGTCGGCGGGGCGCTCGCGGCCTGCCAGCCGCA
CGATCTGGCGGCGCCGTTGGTGGCGCGCATGCTGGCGGATGCCGGGTTGCCGGCCGAGGCCGTCGATGCCGTGGTGCTGG
GCAACGCCCTGGGAGCCGGGGGCAACCCTGCCCGCATGCTGGCGCTGGCGGCCAGCCTCCCCGAGGGCATTCCCGCCGTG
ACGCTGGACAGCCAGTGCTGCGCGGGGCTGGATGCCGTCACCCATGCCTGCGGTCTGCTGGCGCTGGGCCAGGCCGATGT
CGTGGTTGCCGGCGGGGCCGAGGCGTGGAGCCGCGCACCCCTGCGCATGCATCGGCCTCTGCAGGCGGAGGAGGCACCCG
TGCCCTATGACCGGCCACCCTTCACGCCCTGGCCCGCGCGCGACCCCGACATGCTGCTGGCCGCGCTGGACGGCGCGCGG
CGCCTGGGGCTCACGCGCCACGCGCAGGATGCGTACGCCATGGCCAGCCACGCGCGCAGCGTGGCGGCGCGCGCTGCCAT
GGCCCATGAAATCGTGCCGCTGCGGGGGTTGGCGCAAGACGCCTACCCGCGCCTGTTGAGCGCCGCGCGGGTGGCGCGCA
TGCCTGCGGTGGCCAGCGGTCAGTCGGTGGACGGCCAGGATTGCAGCCTCAGCACCGTCGCCGTGTCGCCCAAGGCGGAC
GGCGCGGCCCTGCTGCTGTTGGCCACGCCCTCGGCCTGCGCGCGCTGGGGCGTGCGGCCGCGTGCGCAGTGGCGCGGCAG
CGTCAGCGTGGGTGGCGCGCCCGAGTCGCCCATGCTCTGCGCCGTCGACGTGGCACGCGCCGCGCAGCAACGCTGTGGCT
GGGATTGGGCGGCGCTGGAGGCGCTGGAACTGCACGACGCCTTTGCGGCGCAAGGCCTGGCGTTCTGCAATGCGCTGGGC
TTGCCGCTCGGTGCCATCAACCGGGGCGGGGGTGGCCTGGCGCGCGGGCACCCCATCGGTGCATCGGGCGCCATTGCGCT
GGTGCGCGTGCTGGCGCAGATGGCGCAGCGGCAGGGTGCCGACCGCACCGACCGCACGCTGCGCGGCATGGCGTGCATTG
CGGGTGCGGGTGGCCTGGGTGCGGCCACCTGGGTGGAAATGCCGGAGTGA

Upstream 100 bases:

>100_bases
TGTGGGTGGGCGACTGGCCGCAGACGCCCAGCGGCAAGACCGACCACACAGCCATCGCCCGCGCGCTGCAGGCGCCGTCG
CCCCCGCTGGAGCGCTGGCG

Downstream 100 bases:

>100_bases
GAATGACCGAACCGCTGGTGATGCCTGTGGCGCAGGACGCCTTCGAGACGCTGGCGCAGGCGCGCCATTCCGTGCGCGCC
TTCCTGCCGCGGCCCGTGCC

Product: acetyl-CoA acetyltransferase

Products: Acetoacetyl-CoA; Coenzyme A [C]

Alternate protein names: NA

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNAIPILAWARTPVAPVGGALAACQPHDLAAPLVARMLADAGLPAEAVDAVVLGNALGAGGNPARMLALAASLPEGIPAV
TLDSQCCAGLDAVTHACGLLALGQADVVVAGGAEAWSRAPLRMHRPLQAEEAPVPYDRPPFTPWPARDPDMLLAALDGAR
RLGLTRHAQDAYAMASHARSVAARAAMAHEIVPLRGLAQDAYPRLLSAARVARMPAVASGQSVDGQDCSLSTVAVSPKAD
GAALLLLATPSACARWGVRPRAQWRGSVSVGGAPESPMLCAVDVARAAQQRCGWDWAALEALELHDAFAAQGLAFCNALG
LPLGAINRGGGGLARGHPIGASGAIALVRVLAQMAQRQGADRTDRTLRGMACIAGAGGLGAATWVEMPE

Sequences:

>Translated_389_residues
MNAIPILAWARTPVAPVGGALAACQPHDLAAPLVARMLADAGLPAEAVDAVVLGNALGAGGNPARMLALAASLPEGIPAV
TLDSQCCAGLDAVTHACGLLALGQADVVVAGGAEAWSRAPLRMHRPLQAEEAPVPYDRPPFTPWPARDPDMLLAALDGAR
RLGLTRHAQDAYAMASHARSVAARAAMAHEIVPLRGLAQDAYPRLLSAARVARMPAVASGQSVDGQDCSLSTVAVSPKAD
GAALLLLATPSACARWGVRPRAQWRGSVSVGGAPESPMLCAVDVARAAQQRCGWDWAALEALELHDAFAAQGLAFCNALG
LPLGAINRGGGGLARGHPIGASGAIALVRVLAQMAQRQGADRTDRTLRGMACIAGAGGLGAATWVEMPE
>Mature_389_residues
MNAIPILAWARTPVAPVGGALAACQPHDLAAPLVARMLADAGLPAEAVDAVVLGNALGAGGNPARMLALAASLPEGIPAV
TLDSQCCAGLDAVTHACGLLALGQADVVVAGGAEAWSRAPLRMHRPLQAEEAPVPYDRPPFTPWPARDPDMLLAALDGAR
RLGLTRHAQDAYAMASHARSVAARAAMAHEIVPLRGLAQDAYPRLLSAARVARMPAVASGQSVDGQDCSLSTVAVSPKAD
GAALLLLATPSACARWGVRPRAQWRGSVSVGGAPESPMLCAVDVARAAQQRCGWDWAALEALELHDAFAAQGLAFCNALG
LPLGAINRGGGGLARGHPIGASGAIALVRVLAQMAQRQGADRTDRTLRGMACIAGAGGLGAATWVEMPE

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI148539872, Length=405, Percent_Identity=29.3827160493827, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=26.8948655256724, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI167614485, Length=398, Percent_Identity=25.3768844221106, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4504327, Length=424, Percent_Identity=27.122641509434, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=27.020202020202, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI87082165, Length=407, Percent_Identity=32.6781326781327, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1787663, Length=362, Percent_Identity=33.4254143646409, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1788554, Length=411, Percent_Identity=32.360097323601, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI48994986, Length=402, Percent_Identity=30.8457711442786, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=25.3588516746411, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI133906874, Length=393, Percent_Identity=26.4631043256997, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25147385, Length=372, Percent_Identity=25.2688172043011, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17535921, Length=360, Percent_Identity=26.1111111111111, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6325229, Length=415, Percent_Identity=28.1927710843373, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6322031, Length=336, Percent_Identity=26.7857142857143, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=31.0945273631841, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24640423, Length=403, Percent_Identity=27.2952853598015, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137578, Length=435, Percent_Identity=28.0459770114943, Blast_Score=104, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17648125, Length=396, Percent_Identity=23.989898989899, Blast_Score=102, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.9 [C]

Molecular weight: Translated: 39602; Mature: 39602

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAIPILAWARTPVAPVGGALAACQPHDLAAPLVARMLADAGLPAEAVDAVVLGNALGAG
CCCCEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
GNPARMLALAASLPEGIPAVTLDSQCCAGLDAVTHACGLLALGQADVVVAGGAEAWSRAP
CCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEECCCCEEEECCHHHHHCCC
LRMHRPLQAEEAPVPYDRPPFTPWPARDPDMLLAALDGARRLGLTRHAQDAYAMASHARS
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VAARAAMAHEIVPLRGLAQDAYPRLLSAARVARMPAVASGQSVDGQDCSLSTVAVSPKAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCC
GAALLLLATPSACARWGVRPRAQWRGSVSVGGAPESPMLCAVDVARAAQQRCGWDWAALE
CCEEEEEECCHHHHHCCCCCCHHCCCCEEECCCCCCCCEEHHHHHHHHHHHCCCCHHHHH
ALELHDAFAAQGLAFCNALGLPLGAINRGGGGLARGHPIGASGAIALVRVLAQMAQRQGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DRTDRTLRGMACIAGAGGLGAATWVEMPE
CHHHHHHHHHHHEECCCCCCCCCEEECCC
>Mature Secondary Structure
MNAIPILAWARTPVAPVGGALAACQPHDLAAPLVARMLADAGLPAEAVDAVVLGNALGAG
CCCCEEEEECCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCC
GNPARMLALAASLPEGIPAVTLDSQCCAGLDAVTHACGLLALGQADVVVAGGAEAWSRAP
CCHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHHEECCCCEEEECCHHHHHCCC
LRMHRPLQAEEAPVPYDRPPFTPWPARDPDMLLAALDGARRLGLTRHAQDAYAMASHARS
HHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
VAARAAMAHEIVPLRGLAQDAYPRLLSAARVARMPAVASGQSVDGQDCSLSTVAVSPKAD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCC
GAALLLLATPSACARWGVRPRAQWRGSVSVGGAPESPMLCAVDVARAAQQRCGWDWAALE
CCEEEEEECCHHHHHCCCCCCHHCCCCEEECCCCCCCCEEHHHHHHHHHHHCCCCHHHHH
ALELHDAFAAQGLAFCNALGLPLGAINRGGGGLARGHPIGASGAIALVRVLAQMAQRQGA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
DRTDRTLRGMACIAGAGGLGAATWVEMPE
CHHHHHHHHHHHEECCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acetyl-CoA [C]

Specific reaction: (2) Acetyl-CoA <==> Acetoacetyl-CoA + Coenzyme A [C]

General reaction: Acyl group transfer; Condensation; Thiolytic cleavage [C]

Inhibitor: Acetoacetyl-CoA; Borohydride; Iodoacetamide; N-Ethyl maleimide; p-Chloromercuribenzoate; Sulfhydryl reagents [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]