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The map label for this gene is yhfT [H]

Identifier: 222110738

GI number: 222110738

Start: 1623279

End: 1624763

Strand: Direct

Name: yhfT [H]

Synonym: Dtpsy_1541

Alternate gene names: 222110738

Gene position: 1623279-1624763 (Clockwise)

Preceding gene: 222110737

Following gene: 222110739

Centisome position: 42.76

GC content: 72.59

Gene sequence:

>1485_bases
TTGAACACCCACCCTTGCAAGCGGCAGGCGCTGGCGCCCCTGCCGCAACTGCTGACGGGCGATGCGCTGCCTGAGCTGTT
CGAGTGGGTACATGGCCCCCTGGCGCACTGGGCCCGCACGCGGCCGCACTCCCTGGCGCTGGCCAATGAAACCACCCAGT
GGGACTTCGCGACGCTGCACGACCGTGTGCAGACGCGGGCGCGCTACTGGGATGCGCAAGGCGCGCCGGCCACCGTGCTG
CTGCCCGGTGATGCCGGCACGCTGGAGGGGGTGGTGGAATTCCTGGGCGTGATCGCCAGTGGGCGCTGCGCGGCGATGGG
CGATCCCCAGTGGCCGGCGGGTGTGGCCCGGGCGGTTGCCGAGCGGCTGCCTCTGGGCTCCGGCGGCCCGGCAGCGGCCA
CGCCGCAGTCGCCGTTTTACGTGGGCTTCACCTCGGGCAGCACGGGCCTGCCCAAGGGCTTCATGCGCCACCACCGCTCC
TGGGTGGAGAGCTTTCGCGTCAGCCTGCAGGACTTTGGTGTCGCGGCGGCCACGCGTGTGCTGGCGCCGGGCCGCATGTC
GCATTCGTTGTTCCTGTTCGGCGCGCTGCTGGGCCTGTGGACGGGCGCTGGCGCGGTGCTGCAGGAGCGCTTTTCCGCGC
CGCGCGCGCTGGACACCCTGCGCGCGGGCGGCGCGCCCTTGCTGGTGGCGGTGCCCAGCCAGCTTGTGCTGATGCTGCAG
TGGGCCGAGCGGCGGCAGCTGGCACCGATCGAGGGGGTGGAGCTGGTGCTGATCAGCGGCGCGCGCTGGCTGCGCGAGCG
CACACCCGCGCTGCGCCGGTTGTTCCCGCGCGCACGCATCGTGGAGTTCTACGGTGCATCCGAGGCCAGCTACGTCGCCT
GGATGGATGCAGAGCCGGGCGCGCCGGCCCAGGCCGTGGGCCGGCCGTTCAGCAACGTGGACCTGCACATCGGCCCCGAT
CCCCGCAGCGCCGCGCTGCCGCAGGGCGTGCCGGGGCTGATCTGGGTGCGCAGTCCAATGCTGTTCATGGATTACGTGAA
CGCCGCCGACGGCACCGCCGCCCTGCGCGAGGGCGACTGGCTCTCGGTGCGCGACATGGGTTACGTGGACGACAGCGGTT
TGCTGCACCTGTGCGGTCGCGAGAACCGCATGCTCGTCACCCAAGGCAAGAACCTGTTCCCCGAGGAAGTGGAGGCGCGC
CTGCTGGCCCACCCACAGGTGGCGCAGGCCAGCCTGCAGGGTGTGGCCGACGGCCTGCGCGGCCTGCGTGTGCAGGCGGT
GCTGCAGTGGCAGGGCGACGCGCCGCCACCCGGAGCGCTGGAGCTTGCGGCCTGGTGCCGCGCGGTGTTGGAGCCTTACA
AGGCGCCGCGCCACTGGTGGGTGTGGGTGGGCGACTGGCCGCAGACGCCCAGCGGCAAGACCGACCACACAGCCATCGCC
CGCGCGCTGCAGGCGCCGTCGCCCCCGCTGGAGCGCTGGCGGTGA

Upstream 100 bases:

>100_bases
TCTTCGTGCCCGGTGACCTGGTCAAGTGCGCGCTCTGCGCGGTGGTGGTGCATACCGTGGCCAAGGCGCTGCCAGACTGG
CCGTTTGGTGGGCGCGAACG

Downstream 100 bases:

>100_bases
ACGCTATTCCCATATTGGCCTGGGCGCGCACGCCGGTGGCGCCGGTCGGCGGGGCGCTCGCGGCCTGCCAGCCGCACGAT
CTGGCGGCGCCGTTGGTGGC

Product: AMP-dependent synthetase and ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MNTHPCKRQALAPLPQLLTGDALPELFEWVHGPLAHWARTRPHSLALANETTQWDFATLHDRVQTRARYWDAQGAPATVL
LPGDAGTLEGVVEFLGVIASGRCAAMGDPQWPAGVARAVAERLPLGSGGPAAATPQSPFYVGFTSGSTGLPKGFMRHHRS
WVESFRVSLQDFGVAAATRVLAPGRMSHSLFLFGALLGLWTGAGAVLQERFSAPRALDTLRAGGAPLLVAVPSQLVLMLQ
WAERRQLAPIEGVELVLISGARWLRERTPALRRLFPRARIVEFYGASEASYVAWMDAEPGAPAQAVGRPFSNVDLHIGPD
PRSAALPQGVPGLIWVRSPMLFMDYVNAADGTAALREGDWLSVRDMGYVDDSGLLHLCGRENRMLVTQGKNLFPEEVEAR
LLAHPQVAQASLQGVADGLRGLRVQAVLQWQGDAPPPGALELAAWCRAVLEPYKAPRHWWVWVGDWPQTPSGKTDHTAIA
RALQAPSPPLERWR

Sequences:

>Translated_494_residues
MNTHPCKRQALAPLPQLLTGDALPELFEWVHGPLAHWARTRPHSLALANETTQWDFATLHDRVQTRARYWDAQGAPATVL
LPGDAGTLEGVVEFLGVIASGRCAAMGDPQWPAGVARAVAERLPLGSGGPAAATPQSPFYVGFTSGSTGLPKGFMRHHRS
WVESFRVSLQDFGVAAATRVLAPGRMSHSLFLFGALLGLWTGAGAVLQERFSAPRALDTLRAGGAPLLVAVPSQLVLMLQ
WAERRQLAPIEGVELVLISGARWLRERTPALRRLFPRARIVEFYGASEASYVAWMDAEPGAPAQAVGRPFSNVDLHIGPD
PRSAALPQGVPGLIWVRSPMLFMDYVNAADGTAALREGDWLSVRDMGYVDDSGLLHLCGRENRMLVTQGKNLFPEEVEAR
LLAHPQVAQASLQGVADGLRGLRVQAVLQWQGDAPPPGALELAAWCRAVLEPYKAPRHWWVWVGDWPQTPSGKTDHTAIA
RALQAPSPPLERWR
>Mature_494_residues
MNTHPCKRQALAPLPQLLTGDALPELFEWVHGPLAHWARTRPHSLALANETTQWDFATLHDRVQTRARYWDAQGAPATVL
LPGDAGTLEGVVEFLGVIASGRCAAMGDPQWPAGVARAVAERLPLGSGGPAAATPQSPFYVGFTSGSTGLPKGFMRHHRS
WVESFRVSLQDFGVAAATRVLAPGRMSHSLFLFGALLGLWTGAGAVLQERFSAPRALDTLRAGGAPLLVAVPSQLVLMLQ
WAERRQLAPIEGVELVLISGARWLRERTPALRRLFPRARIVEFYGASEASYVAWMDAEPGAPAQAVGRPFSNVDLHIGPD
PRSAALPQGVPGLIWVRSPMLFMDYVNAADGTAALREGDWLSVRDMGYVDDSGLLHLCGRENRMLVTQGKNLFPEEVEAR
LLAHPQVAQASLQGVADGLRGLRVQAVLQWQGDAPPPGALELAAWCRAVLEPYKAPRHWWVWVGDWPQTPSGKTDHTAIA
RALQAPSPPLERWR

Specific function: May be involved in fatty acid metabolism [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=348, Percent_Identity=23.8505747126437, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI157311624, Length=352, Percent_Identity=26.4204545454545, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI157311622, Length=352, Percent_Identity=26.4204545454545, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI115511026, Length=374, Percent_Identity=27.0053475935829, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI38505220, Length=359, Percent_Identity=23.9554317548747, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI58082049, Length=203, Percent_Identity=30.0492610837438, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI122937307, Length=371, Percent_Identity=23.1805929919137, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI145693145, Length=342, Percent_Identity=27.4853801169591, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=23.0113636363636, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786810, Length=353, Percent_Identity=25.2124645892351, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=25.8160237388724, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71994694, Length=360, Percent_Identity=25.8333333333333, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71994703, Length=360, Percent_Identity=25.8333333333333, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994690, Length=360, Percent_Identity=25.8333333333333, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17531443, Length=390, Percent_Identity=23.5897435897436, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17559526, Length=372, Percent_Identity=23.1182795698925, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538037, Length=383, Percent_Identity=21.1488250652742, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=372, Percent_Identity=23.1182795698925, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=352, Percent_Identity=21.3068181818182, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985884, Length=345, Percent_Identity=22.0289855072464, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32563687, Length=278, Percent_Identity=24.8201438848921, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=349, Percent_Identity=23.7822349570201, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24581924, Length=368, Percent_Identity=25.2717391304348, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24656500, Length=282, Percent_Identity=26.241134751773, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18859661, Length=395, Percent_Identity=23.5443037974684, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922652, Length=347, Percent_Identity=22.478386167147, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 53694; Mature: 53694

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTHPCKRQALAPLPQLLTGDALPELFEWVHGPLAHWARTRPHSLALANETTQWDFATLH
CCCCCCHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCCHHHHHH
DRVQTRARYWDAQGAPATVLLPGDAGTLEGVVEFLGVIASGRCAAMGDPQWPAGVARAVA
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHH
ERLPLGSGGPAAATPQSPFYVGFTSGSTGLPKGFMRHHRSWVESFRVSLQDFGVAAATRV
HHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LAPGRMSHSLFLFGALLGLWTGAGAVLQERFSAPRALDTLRAGGAPLLVAVPSQLVLMLQ
HCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCEEEECHHHHHHHHH
WAERRQLAPIEGVELVLISGARWLRERTPALRRLFPRARIVEFYGASEASYVAWMDAEPG
HHHHCCCCCCCCCEEEEECCHHHHHHCCHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCC
APAQAVGRPFSNVDLHIGPDPRSAALPQGVPGLIWVRSPMLFMDYVNAADGTAALREGDW
CCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCHHHCCCCE
LSVRDMGYVDDSGLLHLCGRENRMLVTQGKNLFPEEVEARLLAHPQVAQASLQGVADGLR
EEECCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLRVQAVLQWQGDAPPPGALELAAWCRAVLEPYKAPRHWWVWVGDWPQTPSGKTDHTAIA
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
RALQAPSPPLERWR
HHHHCCCCCHHHCC
>Mature Secondary Structure
MNTHPCKRQALAPLPQLLTGDALPELFEWVHGPLAHWARTRPHSLALANETTQWDFATLH
CCCCCCHHHHHCCCHHHHCCCHHHHHHHHHCCHHHHHHCCCCCEEEECCCCCCCHHHHHH
DRVQTRARYWDAQGAPATVLLPGDAGTLEGVVEFLGVIASGRCAAMGDPQWPAGVARAVA
HHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHH
ERLPLGSGGPAAATPQSPFYVGFTSGSTGLPKGFMRHHRSWVESFRVSLQDFGVAAATRV
HHCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
LAPGRMSHSLFLFGALLGLWTGAGAVLQERFSAPRALDTLRAGGAPLLVAVPSQLVLMLQ
HCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCEEEECHHHHHHHHH
WAERRQLAPIEGVELVLISGARWLRERTPALRRLFPRARIVEFYGASEASYVAWMDAEPG
HHHHCCCCCCCCCEEEEECCHHHHHHCCHHHHHHCCHHHHHHHCCCCCCCEEEEECCCCC
APAQAVGRPFSNVDLHIGPDPRSAALPQGVPGLIWVRSPMLFMDYVNAADGTAALREGDW
CCHHHHCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCCCCHHHCCCCE
LSVRDMGYVDDSGLLHLCGRENRMLVTQGKNLFPEEVEARLLAHPQVAQASLQGVADGLR
EEECCCCCCCCCCCEEECCCCCCEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
GLRVQAVLQWQGDAPPPGALELAAWCRAVLEPYKAPRHWWVWVGDWPQTPSGKTDHTAIA
CCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
RALQAPSPPLERWR
HHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]