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The map label for this gene is pstA [H]

Identifier: 222110685

GI number: 222110685

Start: 1562447

End: 1563328

Strand: Direct

Name: pstA [H]

Synonym: Dtpsy_1488

Alternate gene names: 222110685

Gene position: 1562447-1563328 (Clockwise)

Preceding gene: 222110684

Following gene: 222110686

Centisome position: 41.15

GC content: 63.27

Gene sequence:

>882_bases
ATGAGCACGTCGCAAAAACTCATCTCCGCGGCCGAGCTGGCCCAGGTGCGCCAGGCGCGCTATACGTCGCGCAAGCGCCT
GAACCAGATCGCATTGACGCTGTCGCTTCTGGCCATGGCGTTTGGCGTGTTCTGGCTGGTCTGGATCCTGTGGGAAACCA
TCCGGCTGGGCGTGGGCGGGCTGAGCGTGGCGCTGTTCACGCAGATGACGCCCCCGCCCAACGAAGCCGGTGGTATCGCG
AACGCGATCTTCGGTTCCTTCGTCATGGTGCTGCTGGCGACGTTCGTGGGCACGCCCATCGGCATCATGGCGGGCGTCTA
CCTTGCGGAATACGACGCACGCGGCTGGCTGGCATCCACGACGCGCTTTGTGAATGACATCCTCCTGTCCGCACCCAGCA
TCGTGATCGGCTTGTTTGTTTATGCCGTGGTGGTCGCCCAGTTCAAGAGCTTCTCGGCCTACGCCGGGATCATGGCCTTG
GCGCTCATCGTCATCCCCGTGGTGATCCGCACCACCGAAAACATGCTGATCCTCGTTCCGGCCAGTCTGCGCGAGGCGGC
CTACGCCCTGGGCACACCCAAATGGAAGGTCATCACCATGGTGACGCTGCGCGCGGCCCGCGCCGGCGTGATCACCGGCA
TATTGCTGGCGGTGGCACGCATCGCAGGCGAGACGGCGCCCCTGCTGTTCACTGCACTGAACAACCAGTTCTGGAATGGC
GACCTGTCCAAGCCCATGGCCAGCTTGCCGGTGACCATCTTCAAGTTCGCCATGAGCCCCTATGAGAACTGGCAGCAGCT
CGCCTGGGCTGGCGTGTTCCTCATCACCCTTGCGGTGCTGGGCCTGAACATCCTGGCCCGTGTGGTCACGCGCCAAAAGT
AA

Upstream 100 bases:

>100_bases
TGATCTATCTGGGACTGGTGCTCTTCTTCATCACTTTCGTGGTGCTGGCCTTGTCCAAGCTGCTGCTGGCGCGCCTCAAG
AAAAACGAAGGGGCCCGCGC

Downstream 100 bases:

>100_bases
CTATTGCCTCCGGAAGACATTCATGCCCTCCACCAAGACCTCCGCGCCCGTGCAATCCAAGATCACGGTCCGTGACCTGA
ACTTCTATTACGGGAAGTTC

Product: phosphate transporter permease subunit ptsa

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MSTSQKLISAAELAQVRQARYTSRKRLNQIALTLSLLAMAFGVFWLVWILWETIRLGVGGLSVALFTQMTPPPNEAGGIA
NAIFGSFVMVLLATFVGTPIGIMAGVYLAEYDARGWLASTTRFVNDILLSAPSIVIGLFVYAVVVAQFKSFSAYAGIMAL
ALIVIPVVIRTTENMLILVPASLREAAYALGTPKWKVITMVTLRAARAGVITGILLAVARIAGETAPLLFTALNNQFWNG
DLSKPMASLPVTIFKFAMSPYENWQQLAWAGVFLITLAVLGLNILARVVTRQK

Sequences:

>Translated_293_residues
MSTSQKLISAAELAQVRQARYTSRKRLNQIALTLSLLAMAFGVFWLVWILWETIRLGVGGLSVALFTQMTPPPNEAGGIA
NAIFGSFVMVLLATFVGTPIGIMAGVYLAEYDARGWLASTTRFVNDILLSAPSIVIGLFVYAVVVAQFKSFSAYAGIMAL
ALIVIPVVIRTTENMLILVPASLREAAYALGTPKWKVITMVTLRAARAGVITGILLAVARIAGETAPLLFTALNNQFWNG
DLSKPMASLPVTIFKFAMSPYENWQQLAWAGVFLITLAVLGLNILARVVTRQK
>Mature_292_residues
STSQKLISAAELAQVRQARYTSRKRLNQIALTLSLLAMAFGVFWLVWILWETIRLGVGGLSVALFTQMTPPPNEAGGIAN
AIFGSFVMVLLATFVGTPIGIMAGVYLAEYDARGWLASTTRFVNDILLSAPSIVIGLFVYAVVVAQFKSFSAYAGIMALA
LIVIPVVIRTTENMLILVPASLREAAYALGTPKWKVITMVTLRAARAGVITGILLAVARIAGETAPLLFTALNNQFWNGD
LSKPMASLPVTIFKFAMSPYENWQQLAWAGVFLITLAVLGLNILARVVTRQK

Specific function: Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0581

COG function: function code P; ABC-type phosphate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790163, Length=285, Percent_Identity=66.3157894736842, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1790164, Length=212, Percent_Identity=28.3018867924528, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005672 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31711; Mature: 31580

Theoretical pI: Translated: 10.79; Mature: 10.79

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTSQKLISAAELAQVRQARYTSRKRLNQIALTLSLLAMAFGVFWLVWILWETIRLGVGG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LSVALFTQMTPPPNEAGGIANAIFGSFVMVLLATFVGTPIGIMAGVYLAEYDARGWLAST
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
TRFVNDILLSAPSIVIGLFVYAVVVAQFKSFSAYAGIMALALIVIPVVIRTTENMLILVP
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
ASLREAAYALGTPKWKVITMVTLRAARAGVITGILLAVARIAGETAPLLFTALNNQFWNG
CHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
DLSKPMASLPVTIFKFAMSPYENWQQLAWAGVFLITLAVLGLNILARVVTRQK
CCCCHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STSQKLISAAELAQVRQARYTSRKRLNQIALTLSLLAMAFGVFWLVWILWETIRLGVGG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
LSVALFTQMTPPPNEAGGIANAIFGSFVMVLLATFVGTPIGIMAGVYLAEYDARGWLAST
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHH
TRFVNDILLSAPSIVIGLFVYAVVVAQFKSFSAYAGIMALALIVIPVVIRTTENMLILVP
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEC
ASLREAAYALGTPKWKVITMVTLRAARAGVITGILLAVARIAGETAPLLFTALNNQFWNG
CHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCC
DLSKPMASLPVTIFKFAMSPYENWQQLAWAGVFLITLAVLGLNILARVVTRQK
CCCCHHHHCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2993631; 3881386; 7686882; 9278503; 1447208 [H]