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The map label for this gene is pheS

Identifier: 222110650

GI number: 222110650

Start: 1518889

End: 1519941

Strand: Direct

Name: pheS

Synonym: Dtpsy_1452

Alternate gene names: 222110650

Gene position: 1518889-1519941 (Clockwise)

Preceding gene: 222110649

Following gene: 222110651

Centisome position: 40.01

GC content: 65.91

Gene sequence:

>1053_bases
ATGAACGAGTTGGATTCCTTGGTGACGAGCGCGCGCGAGTCGTTCGCGCGTTCCGCAACCCCTGCCGAACTGGAGAACGC
CAAGGCGCAGTTCCTGGGCAAGTCAGGCCGCCTGACCGAGCTCATGAAGGGCATGGCGCAGCTCTCGCCCGAGGAGAAGA
AAACCCGCGGCGCCGCCATCAACGTGGCCAAGCAGGCCGTGGAGGCAGCCCTCGCCGCACGCCGCCAGGAGCTGGCGGAC
GCCGAGCTGCAGGCACAGCTCAAGGCCGAGGCGCTGGATGTGACACTGCCGGGCCGCCAGCGCGGCCGCGGCGGCCTGCA
TCCCGTGTCGCTGACGCTGGAGCGCATCGAGCGGATCTTCGGTTCGATGGGCTTCGAGGTGGCGGACGGCCCCGAGATCG
AGACCGACTGGTTCAACTTCACGGCGCTGAACACGCCCGAGGACCACCCCGCCCGGTCCATGCACGACACCTTCTACGTG
GAAGGCGGCACACCCGAAGCCCCCAACCTGCTGCGCACGCACACCAGCCCGATGCAGGTGCGCTACGCCGTGCAGCATGT
GAAGAAACACCGTGGCCTGATCGACGCCGGCCAGGCCATGCCCGAGATCCGCGTGATCGCTCCGGGCCGCACCTACCGCG
TGGACTCCGACGCCACGCACTCGCCCATGTTCCACCAATGTGAAGGCCTGTGGATCGGCGAGAACGTGAGCTTCAAGGAC
CTGAAGGTCGTGTTCACGGACTTCTGCAAGACCTTCTTTGAAAGCGATGACCTGGTGCTGCGCTTTCGTCCCAGCTTCTT
CCCTTTCACCGAGCCCAGCGCCGAGATCGATATCCAGTTCCAGTCGGGCCCGTTGGCCGGCAAGTGGCTGGAGGTGGCGG
GCTCCGGCCAGGTGCACCCCAACGTGGTGCGCAACATGGGGCTGGACCCCGAACGCTTCATCGGCTTTGCCTTCGGCATG
GGGCCGGACCGACTGACCATGCTGCGCTACGGCGTGAACGACCTGCGCCTGTTCTTCGACGGCGACATCCGTTTCCTGTC
GCAGTTCCAGTAA

Upstream 100 bases:

>100_bases
TCACGATTGGTAGCTATAAATTGTATAGCTACTAGCGCGCAAACAACAAGGGCTAGGGCTTGAAAAGGCACTAGCCCTTG
TTTCTTTTTCAAGAGTCGAT

Downstream 100 bases:

>100_bases
TGCAAAAAAGTGAGCTGCCAGCGCTTGTTAAGCCTAATCTTCAATATGTTTCCATATGGAAAACAATAAGTAGCAAGCGC
TAGCAGCTCTCTTTTCAAGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MNELDSLVTSARESFARSATPAELENAKAQFLGKSGRLTELMKGMAQLSPEEKKTRGAAINVAKQAVEAALAARRQELAD
AELQAQLKAEALDVTLPGRQRGRGGLHPVSLTLERIERIFGSMGFEVADGPEIETDWFNFTALNTPEDHPARSMHDTFYV
EGGTPEAPNLLRTHTSPMQVRYAVQHVKKHRGLIDAGQAMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
LKVVFTDFCKTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQSGPLAGKWLEVAGSGQVHPNVVRNMGLDPERFIGFAFGM
GPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ

Sequences:

>Translated_350_residues
MNELDSLVTSARESFARSATPAELENAKAQFLGKSGRLTELMKGMAQLSPEEKKTRGAAINVAKQAVEAALAARRQELAD
AELQAQLKAEALDVTLPGRQRGRGGLHPVSLTLERIERIFGSMGFEVADGPEIETDWFNFTALNTPEDHPARSMHDTFYV
EGGTPEAPNLLRTHTSPMQVRYAVQHVKKHRGLIDAGQAMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
LKVVFTDFCKTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQSGPLAGKWLEVAGSGQVHPNVVRNMGLDPERFIGFAFGM
GPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ
>Mature_350_residues
MNELDSLVTSARESFARSATPAELENAKAQFLGKSGRLTELMKGMAQLSPEEKKTRGAAINVAKQAVEAALAARRQELAD
AELQAQLKAEALDVTLPGRQRGRGGLHPVSLTLERIERIFGSMGFEVADGPEIETDWFNFTALNTPEDHPARSMHDTFYV
EGGTPEAPNLLRTHTSPMQVRYAVQHVKKHRGLIDAGQAMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
LKVVFTDFCKTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQSGPLAGKWLEVAGSGQVHPNVVRNMGLDPERFIGFAFGM
GPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=301, Percent_Identity=31.8936877076412, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI4758340, Length=269, Percent_Identity=30.8550185873606, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1788007, Length=350, Percent_Identity=53.1428571428571, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17508957, Length=276, Percent_Identity=31.8840579710145, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32563657, Length=276, Percent_Identity=31.8840579710145, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566635, Length=261, Percent_Identity=32.5670498084291, Blast_Score=107, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=372, Percent_Identity=26.6129032258064, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6325304, Length=93, Percent_Identity=39.7849462365591, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI18858079, Length=290, Percent_Identity=31.0344827586207, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17137424, Length=301, Percent_Identity=28.2392026578073, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ACIET (B9MHY2)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552914.1
- ProteinModelPortal:   B9MHY2
- SMR:   B9MHY2
- GeneID:   7385093
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1452
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 39021; Mature: 39021

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELDSLVTSARESFARSATPAELENAKAQFLGKSGRLTELMKGMAQLSPEEKKTRGAAI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCHHH
NVAKQAVEAALAARRQELADAELQAQLKAEALDVTLPGRQRGRGGLHPVSLTLERIERIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GSMGFEVADGPEIETDWFNFTALNTPEDHPARSMHDTFYVEGGTPEAPNLLRTHTSPMQV
HHCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCCHHHH
RYAVQHVKKHRGLIDAGQAMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
HHHHHHHHHHCCCHHHHCCCCCEEEECCCCEEEECCCCCCCCHHHHCCCEEECCCCCHHH
LKVVFTDFCKTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQSGPLAGKWLEVAGSGQVHP
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCH
NVVRNMGLDPERFIGFAFGMGPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ
HHHHHCCCCHHHHHEEHCCCCCCHHHHHHHCCCEEEEEECCCCHHHHCCC
>Mature Secondary Structure
MNELDSLVTSARESFARSATPAELENAKAQFLGKSGRLTELMKGMAQLSPEEKKTRGAAI
CCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHCCHHH
NVAKQAVEAALAARRQELADAELQAQLKAEALDVTLPGRQRGRGGLHPVSLTLERIERIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GSMGFEVADGPEIETDWFNFTALNTPEDHPARSMHDTFYVEGGTPEAPNLLRTHTSPMQV
HHCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCCHHHH
RYAVQHVKKHRGLIDAGQAMPEIRVIAPGRTYRVDSDATHSPMFHQCEGLWIGENVSFKD
HHHHHHHHHHCCCHHHHCCCCCEEEECCCCEEEECCCCCCCCHHHHCCCEEECCCCCHHH
LKVVFTDFCKTFFESDDLVLRFRPSFFPFTEPSAEIDIQFQSGPLAGKWLEVAGSGQVHP
HHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECCCCCCCCEEEECCCCCCCH
NVVRNMGLDPERFIGFAFGMGPDRLTMLRYGVNDLRLFFDGDIRFLSQFQ
HHHHHCCCCHHHHHEEHCCCCCCHHHHHHHCCCEEEEEECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA