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The map label for this gene is rumA [H]

Identifier: 222110633

GI number: 222110633

Start: 1505106

End: 1506554

Strand: Direct

Name: rumA [H]

Synonym: Dtpsy_1435

Alternate gene names: 222110633

Gene position: 1505106-1506554 (Clockwise)

Preceding gene: 222110632

Following gene: 222110636

Centisome position: 39.64

GC content: 67.7

Gene sequence:

>1449_bases
ATGAGCGACCCCACAGAATTCAGCCCACCACCGCCCACCTCCACGGATCTTCCCCCCGGCTGGCTTGAAGTCACCGCCAT
GGACATGGACGCCCAAGGCGTCGCGCGCAAGCCCGATGGCAAGGTGGTGTTCATCGACGGCGCACTGCCCACCGAGCTGG
TCAGTGCCAATACCCACCGCAAGAAAAACAACTGGGAGCAGGCCAGCCTGACGGAAGTCCACCGCCAGTCCAGCCAGCGC
GTACAGCCCGGCTGCCCGCACTTCGGCCTGCACGCGGGCGCCTGCGGCGGCTGCAAGATGCAGCATCTGCACGTGGCGGC
CCAGGTGGCCGTGAAACAGCGCGTGCTGGAGGACAACCTCTGGCACCTGGGGAAAGTGAAGGCCGAGACCATCCTGCGCC
CCATTGAAGGCCCTGCCTGGGGCTACCGCTACCGCGCGCGCCTGTCCGTGCGCTACGTGCCCAAGAAGGACAAGGTGCTC
GTGGGCTTTCACGAGCGCAAGAGCCGCTACATCGCCGACATGGAGACTTGCAAGATCCTGCCGCCGCATGTGGACGCCAT
GCTGATGCCGCTGCGCGCCCTGATCGGCAGCATGGCCGCACGCGACACCTGCCCGCAGATCGAGCTGGCCTGCGGCGATA
CGGTCACGGCGCTGGTGCTGCGCCACCTGGAGCCGCTGTCCCCGGAGGACAAAGACAAGTTGCGCGCCTTTGCCGCCGCG
CATGGCGTGCAATGGTGGTTGCAGCCCAAGGGGCCGGACACGGTGCATCTGCTGGACGAGGGCGGCGAGCCGCTTGCCTA
TGGGCTGCCCGACTTCGGCATCACCATGCCCTTCAAACCCACCGACTTCACGCAGGTCAACCCGCACATCAACCGCGTGC
TGGTCACGCGCGCGCTGCGCCTGCTGGACGCGCGCAAGGACGAGCGCGTGATCGACTGGTTCTGCGGCCTGGGCAACTTC
ACGCTGCCCATCGCCACCCAGGCGCGCGAGGTGCTGGGCATCGAGGGCAGTGAGGCGCTGGTGGCGCGTTCGCGCGAGAA
TTACCAGTCAAATCAGGCCCAAAGGCAGGATGGATCTGCGCTGGCAGCTACCAGCTTTGTAGCGCGCAACCTGTTCGAGA
TGACGCCTGAGATGCTCATTGCCGATGGCGCCTCCGACAAATGGCTGGTGGACCCGCCGCGCGAGGGGGCGTTCGCCCTG
TCCAAGGCGCTGGCGGACATCCACCAGGCGCGCATCGGTGCCGAGGACGCGCCGCCGCTGCCCGCTGGGCACGAAGGCTG
GCAGCCCCCCCGGCGCATCGTCTACGTGAGCTGCAACCCGGCCACGCTGGCGCGGGACGCGGGCCTGCTGGTGCACCAGG
CGGGCTACCGCTGCGTGGCGGCGGGGGTGGTCAACATGTTTCCGCACACGGCCCATGTGGAGAGCATGGCAGTGTTCGAG
CGGGGCTGA

Upstream 100 bases:

>100_bases
GCTGCAGGCGCCCCCACCGCGCCCCGTGCGCCGCGCGCCAGCCGCAGCTGAAAGCCGCCCGCGCGGCGGCAGGGGTGGGC
CGGCCTGAGACAATCGGGGC

Downstream 100 bases:

>100_bases
CGCCCGCGGTGGCTCAAGACGTTTTTGGCCAGGGGCGCTTGCCTAGCAAGCGCCAGCAGCTATCGTTTGGATAGCATCAG
CTACGGTTGCGGGCGGTGCG

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase [H]

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSDPTEFSPPPPTSTDLPPGWLEVTAMDMDAQGVARKPDGKVVFIDGALPTELVSANTHRKKNNWEQASLTEVHRQSSQR
VQPGCPHFGLHAGACGGCKMQHLHVAAQVAVKQRVLEDNLWHLGKVKAETILRPIEGPAWGYRYRARLSVRYVPKKDKVL
VGFHERKSRYIADMETCKILPPHVDAMLMPLRALIGSMAARDTCPQIELACGDTVTALVLRHLEPLSPEDKDKLRAFAAA
HGVQWWLQPKGPDTVHLLDEGGEPLAYGLPDFGITMPFKPTDFTQVNPHINRVLVTRALRLLDARKDERVIDWFCGLGNF
TLPIATQAREVLGIEGSEALVARSRENYQSNQAQRQDGSALAATSFVARNLFEMTPEMLIADGASDKWLVDPPREGAFAL
SKALADIHQARIGAEDAPPLPAGHEGWQPPRRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTAHVESMAVFE
RG

Sequences:

>Translated_482_residues
MSDPTEFSPPPPTSTDLPPGWLEVTAMDMDAQGVARKPDGKVVFIDGALPTELVSANTHRKKNNWEQASLTEVHRQSSQR
VQPGCPHFGLHAGACGGCKMQHLHVAAQVAVKQRVLEDNLWHLGKVKAETILRPIEGPAWGYRYRARLSVRYVPKKDKVL
VGFHERKSRYIADMETCKILPPHVDAMLMPLRALIGSMAARDTCPQIELACGDTVTALVLRHLEPLSPEDKDKLRAFAAA
HGVQWWLQPKGPDTVHLLDEGGEPLAYGLPDFGITMPFKPTDFTQVNPHINRVLVTRALRLLDARKDERVIDWFCGLGNF
TLPIATQAREVLGIEGSEALVARSRENYQSNQAQRQDGSALAATSFVARNLFEMTPEMLIADGASDKWLVDPPREGAFAL
SKALADIHQARIGAEDAPPLPAGHEGWQPPRRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTAHVESMAVFE
RG
>Mature_481_residues
SDPTEFSPPPPTSTDLPPGWLEVTAMDMDAQGVARKPDGKVVFIDGALPTELVSANTHRKKNNWEQASLTEVHRQSSQRV
QPGCPHFGLHAGACGGCKMQHLHVAAQVAVKQRVLEDNLWHLGKVKAETILRPIEGPAWGYRYRARLSVRYVPKKDKVLV
GFHERKSRYIADMETCKILPPHVDAMLMPLRALIGSMAARDTCPQIELACGDTVTALVLRHLEPLSPEDKDKLRAFAAAH
GVQWWLQPKGPDTVHLLDEGGEPLAYGLPDFGITMPFKPTDFTQVNPHINRVLVTRALRLLDARKDERVIDWFCGLGNFT
LPIATQAREVLGIEGSEALVARSRENYQSNQAQRQDGSALAATSFVARNLFEMTPEMLIADGASDKWLVDPPREGAFALS
KALADIHQARIGAEDAPPLPAGHEGWQPPRRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTAHVESMAVFER
G

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumA subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=287, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI51173878, Length=287, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI1789148, Length=462, Percent_Identity=38.3116883116883, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1787083, Length=414, Percent_Identity=23.9130434782609, Blast_Score=75, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24666579, Length=223, Percent_Identity=29.1479820627803, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.190 [H]

Molecular weight: Translated: 53015; Mature: 52884

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDPTEFSPPPPTSTDLPPGWLEVTAMDMDAQGVARKPDGKVVFIDGALPTELVSANTHR
CCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCC
KKNNWEQASLTEVHRQSSQRVQPGCPHFGLHAGACGGCKMQHLHVAAQVAVKQRVLEDNL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
WHLGKVKAETILRPIEGPAWGYRYRARLSVRYVPKKDKVLVGFHERKSRYIADMETCKIL
HHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCC
PPHVDAMLMPLRALIGSMAARDTCPQIELACGDTVTALVLRHLEPLSPEDKDKLRAFAAA
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
HGVQWWLQPKGPDTVHLLDEGGEPLAYGLPDFGITMPFKPTDFTQVNPHINRVLVTRALR
HCCEEEECCCCCCEEEEECCCCCEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHH
LLDARKDERVIDWFCGLGNFTLPIATQAREVLGIEGSEALVARSRENYQSNQAQRQDGSA
HHHCCCCCHHHHHHHCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCH
LAATSFVARNLFEMTPEMLIADGASDKWLVDPPREGAFALSKALADIHQARIGAEDAPPL
HHHHHHHHHHHHHHCCHHEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PAGHEGWQPPRRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTAHVESMAVFE
CCCCCCCCCCCEEEEEECCCHHHHHCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHCC
RG
CC
>Mature Secondary Structure 
SDPTEFSPPPPTSTDLPPGWLEVTAMDMDAQGVARKPDGKVVFIDGALPTELVSANTHR
CCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEECCCCCHHHHCCCCCC
KKNNWEQASLTEVHRQSSQRVQPGCPHFGLHAGACGGCKMQHLHVAAQVAVKQRVLEDNL
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
WHLGKVKAETILRPIEGPAWGYRYRARLSVRYVPKKDKVLVGFHERKSRYIADMETCKIL
HHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCEEEEEHHHHHHHHHHHHHHCCC
PPHVDAMLMPLRALIGSMAARDTCPQIELACGDTVTALVLRHLEPLSPEDKDKLRAFAAA
CCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHH
HGVQWWLQPKGPDTVHLLDEGGEPLAYGLPDFGITMPFKPTDFTQVNPHINRVLVTRALR
HCCEEEECCCCCCEEEEECCCCCEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHH
LLDARKDERVIDWFCGLGNFTLPIATQAREVLGIEGSEALVARSRENYQSNQAQRQDGSA
HHHCCCCCHHHHHHHCCCCCEEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCH
LAATSFVARNLFEMTPEMLIADGASDKWLVDPPREGAFALSKALADIHQARIGAEDAPPL
HHHHHHHHHHHHHHCCHHEEECCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PAGHEGWQPPRRIVYVSCNPATLARDAGLLVHQAGYRCVAAGVVNMFPHTAHVESMAVFE
CCCCCCCCCCCEEEEEECCCHHHHHCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHCC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA