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The map label for this gene is rnhB

Identifier: 222110440

GI number: 222110440

Start: 1299428

End: 1300057

Strand: Direct

Name: rnhB

Synonym: Dtpsy_1237

Alternate gene names: 222110440

Gene position: 1299428-1300057 (Clockwise)

Preceding gene: 222110439

Following gene: 222110441

Centisome position: 34.23

GC content: 67.94

Gene sequence:

>630_bases
ATGCGATCCAGAACCTCCTTGCTTGAGCAGGCCGCCCTGGACTGGCATCCGCCCGGCCTCGTCGCCGGCGTGGATGAGGC
CGGGCGTGGGCCGTTGGCCGGCCCCGTGGTGGCGGCTGCGGTGATCCTGGACGACCTGCAGCCCATCGCTGGCCTGGCCG
ACTCCAAGGTGCTTACGGCCGCACGGCGCGAGAAACTCTACGACGAGATCCGTGCCAAGGCGCTGTGCTGCAGCATTGCG
GAAGCATCGGTGGAGGAGATCGATCAACACAATATCCTGCAAGCCACGATGCTGGCCATGCGCCGTGCGGTGCTGGGGCT
GCGGCTGAAGCCGGTGCGGGTGTTGGTGGATGGCAATCGCTTGCCGCCGCTGGACGTTCCGGCGGAGGCCATCGTCAAGG
GCGATGCGCTGGTCGCATCGATCTCAGCCGCCTCCATTCTTGCAAAGGTCACGCGCGACCGCTGGTGCGCGCAATTGCAC
CAGCAATACCCGGTGTATGGCTTTGCCGGCCATAAGGGCTACGGCACGGCAGAGCACCTGGCCGCGCTGGAAGTGCATGG
GGCCTGTCCCCAGCACCGGCGCTCGTTCGCGCCGGTGGCGCGCGCCTTGCAGGCGCCCGTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GACGCGCGGTTGCAGGATCCGGCCCGTATCCAACGGCTGGAACAGCGCTTCACGGCATTGCACGACGATCTGCAGCGCGA
TACCCCGCGACTGGCTGCCC

Downstream 100 bases:

>100_bases
GTCTGATCGCTATCCCCCTTTGCGTTTCCTATGCACGCCACGCCTGCTTTCATCCAATCGCGCGACAACGCACTGCTCAA
GGAGTTGCGCCGCCTGTCGC

Product: ribonuclease hii

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MRSRTSLLEQAALDWHPPGLVAGVDEAGRGPLAGPVVAAAVILDDLQPIAGLADSKVLTAARREKLYDEIRAKALCCSIA
EASVEEIDQHNILQATMLAMRRAVLGLRLKPVRVLVDGNRLPPLDVPAEAIVKGDALVASISAASILAKVTRDRWCAQLH
QQYPVYGFAGHKGYGTAEHLAALEVHGACPQHRRSFAPVARALQAPVAA

Sequences:

>Translated_209_residues
MRSRTSLLEQAALDWHPPGLVAGVDEAGRGPLAGPVVAAAVILDDLQPIAGLADSKVLTAARREKLYDEIRAKALCCSIA
EASVEEIDQHNILQATMLAMRRAVLGLRLKPVRVLVDGNRLPPLDVPAEAIVKGDALVASISAASILAKVTRDRWCAQLH
QQYPVYGFAGHKGYGTAEHLAALEVHGACPQHRRSFAPVARALQAPVAA
>Mature_209_residues
MRSRTSLLEQAALDWHPPGLVAGVDEAGRGPLAGPVVAAAVILDDLQPIAGLADSKVLTAARREKLYDEIRAKALCCSIA
EASVEEIDQHNILQATMLAMRRAVLGLRLKPVRVLVDGNRLPPLDVPAEAIVKGDALVASISAASILAKVTRDRWCAQLH
QQYPVYGFAGHKGYGTAEHLAALEVHGACPQHRRSFAPVARALQAPVAA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=63.5869565217391, Blast_Score=214, Evalue=4e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_ACIET (B9MGN1)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552704.1
- ProteinModelPortal:   B9MGN1
- SMR:   B9MGN1
- GeneID:   7383139
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1237
- ProtClustDB:   PRK00015
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22221; Mature: 22221

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRTSLLEQAALDWHPPGLVAGVDEAGRGPLAGPVVAAAVILDDLQPIAGLADSKVLTA
CCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARREKLYDEIRAKALCCSIAEASVEEIDQHNILQATMLAMRRAVLGLRLKPVRVLVDGNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LPPLDVPAEAIVKGDALVASISAASILAKVTRDRWCAQLHQQYPVYGFAGHKGYGTAEHL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHH
AALEVHGACPQHRRSFAPVARALQAPVAA
HHHHHCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRSRTSLLEQAALDWHPPGLVAGVDEAGRGPLAGPVVAAAVILDDLQPIAGLADSKVLTA
CCHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARREKLYDEIRAKALCCSIAEASVEEIDQHNILQATMLAMRRAVLGLRLKPVRVLVDGNR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCC
LPPLDVPAEAIVKGDALVASISAASILAKVTRDRWCAQLHQQYPVYGFAGHKGYGTAEHL
CCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHH
AALEVHGACPQHRRSFAPVARALQAPVAA
HHHHHCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA