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The map label for this gene is glnG [H]

Identifier: 222110325

GI number: 222110325

Start: 1165497

End: 1167032

Strand: Direct

Name: glnG [H]

Synonym: Dtpsy_1112

Alternate gene names: 222110325

Gene position: 1165497-1167032 (Clockwise)

Preceding gene: 222110324

Following gene: 222110326

Centisome position: 30.7

GC content: 70.12

Gene sequence:

>1536_bases
ATGAAGCCGATCTGGATAGTAGACGACGACCCCTCGATCCGCTTCGTCCTCGAAAAGGCGCTGGCGCGCGAAAACCTGCC
CACGCGCAGCTTCACGCAGGCGCGCGATGTACTGGACGCGCTGGCCGACGTAGCCGCGGGCGACCCCACCCACCAGGGAC
CGCAGGTCCTGGTGAGCGATATCCGCATGCCGGGCGGCTCCGGCCTGCAACTGCTGGAGAAGGTGCGCGGCCTGCAACCC
GGTCTGCCGGTCATCGTGATGACGGCGTATTCCGACCTGGACAGCGCCGTGTCCGCCTTCCAAGGCGGCGCCTTTGAATA
CCTGCCCAAGCCGTTCGACCTGCCCAAGGCGGTGGAGCTGATCCGCCGCGCTGTGGAGGAGAGCCAGCGCGAGGAGGTTG
CCGAGCAGCAGCTCACCGCGATGCCCGAGATGCTGGGCCAGGCACCCGCCATGCAGGACGTGTTCCGCGCCATCGGCCGG
CTGAGCCAGAGCGTGGTCACGGTGCTGATCACGGGCGAATCCGGCTCGGGCAAGGAACTCGTGGCACGCGCGCTGCACAA
GCACTCGCCCGTGGCGGGTGGGCCCTTCGTGGCCATCAACACTGCGGCCATCCCGAAGGACCTGCTGGAGAGCGAGCTCT
TCGGCCATGAGCGCGGCGCCTTCACGGGCGCGCAGACGCAGCGGCGCGGGCGCTTTGAGCAGGCCGAGGGCGGCACGCTG
TTCCTCGATGAAATCGGCGACATGCCCTTCGACCTGCAGACGCGCCTGCTGCGCGTGCTGTCGGACGGGCAGTTCTACCG
CGTGGGCGGCCATGCCGCCGTGAAGGCCAATGTGCGCGTGATCGCCGCTACGCACCAGAACCTGGAGCAGCGCGTGAAGG
ATGGGGCCTTCCGCGAGGACCTGTTCCACCGCCTCAATGTGATCCGCCTGCGCCTGCCGGCGCTGCGCGAGCGCAGCGAA
GACGTGGCCATGCTGACGCGCTACTTCCTGCAGCGCAGCGCACGGCAACTGGGCGTGGAGCCCAAGCGCATCTCCGACGC
GGCGCTGGCCCTGCTCACACGCTTTGCCTTCCCAGGCAACGTGCGCCAGCTGGAGAACATCTGCCACTGGCTGACGGTGA
TGGCGCCCGCGCAGGTCATCTCGGCGCAGGACCTGCCGCCCGAGGTGCTGGAGGCCACGCCGCATCCCGCCGCGCCGTTG
CAGGGCGTGGCGGACGAGGTTGCCGCTGGCGCGACGAGCGTGCCGGCCCCCGCGCTGCCGCCTGCGCCGGCGGGCACGCG
CACCGACCAGGCTGCGGCCCTGGATGGCATGGGCTGGGAGCCCGCGCTGGTGAGCGAGGCCCAGCAGCTGCTGCGCAGCG
GCCAGGCCGACGTGTGGGACGTGCTCACGCGGCGTTTCGAGTCGCGCCTGATCCGCACGGCGCTGCAGGCCACGCACGGG
CGGCGCATGGAAGCGGCGCAGCGCCTGGGCATCGGCCGCAACACCATCACGCGCAAGATCCAGGAACTGGGCCTGGATGC
GCACGATGATGAGTGA

Upstream 100 bases:

>100_bases
GAGCCAGCCGGGCCGCACCGACTTCCGCATCCTCATACCACTGCCTTGAGTCCCTGACACGCACGACCCCAACGGGTAAA
GGTAAAGGGAGCTGGAACAC

Downstream 100 bases:

>100_bases
AACAAGGCGCTGGCGCTTTAAGGACAAGCGCTAGTAGCTATTAATTCGATAGCATAAGACCTTGTGGCGCGCATGCACAG
CGTGCGCGTGAGTCCTACAG

Product: nitrogen metabolism transcriptional regulator, ntrc, fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTQARDVLDALADVAAGDPTHQGPQVLVSDIRMPGGSGLQLLEKVRGLQP
GLPVIVMTAYSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRAVEESQREEVAEQQLTAMPEMLGQAPAMQDVFRAIGR
LSQSVVTVLITGESGSGKELVARALHKHSPVAGGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKANVRVIAATHQNLEQRVKDGAFREDLFHRLNVIRLRLPALRERSE
DVAMLTRYFLQRSARQLGVEPKRISDAALALLTRFAFPGNVRQLENICHWLTVMAPAQVISAQDLPPEVLEATPHPAAPL
QGVADEVAAGATSVPAPALPPAPAGTRTDQAAALDGMGWEPALVSEAQQLLRSGQADVWDVLTRRFESRLIRTALQATHG
RRMEAAQRLGIGRNTITRKIQELGLDAHDDE

Sequences:

>Translated_511_residues
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTQARDVLDALADVAAGDPTHQGPQVLVSDIRMPGGSGLQLLEKVRGLQP
GLPVIVMTAYSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRAVEESQREEVAEQQLTAMPEMLGQAPAMQDVFRAIGR
LSQSVVTVLITGESGSGKELVARALHKHSPVAGGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKANVRVIAATHQNLEQRVKDGAFREDLFHRLNVIRLRLPALRERSE
DVAMLTRYFLQRSARQLGVEPKRISDAALALLTRFAFPGNVRQLENICHWLTVMAPAQVISAQDLPPEVLEATPHPAAPL
QGVADEVAAGATSVPAPALPPAPAGTRTDQAAALDGMGWEPALVSEAQQLLRSGQADVWDVLTRRFESRLIRTALQATHG
RRMEAAQRLGIGRNTITRKIQELGLDAHDDE
>Mature_511_residues
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTQARDVLDALADVAAGDPTHQGPQVLVSDIRMPGGSGLQLLEKVRGLQP
GLPVIVMTAYSDLDSAVSAFQGGAFEYLPKPFDLPKAVELIRRAVEESQREEVAEQQLTAMPEMLGQAPAMQDVFRAIGR
LSQSVVTVLITGESGSGKELVARALHKHSPVAGGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKANVRVIAATHQNLEQRVKDGAFREDLFHRLNVIRLRLPALRERSE
DVAMLTRYFLQRSARQLGVEPKRISDAALALLTRFAFPGNVRQLENICHWLTVMAPAQVISAQDLPPEVLEATPHPAAPL
QGVADEVAAGATSVPAPALPPAPAGTRTDQAAALDGMGWEPALVSEAQQLLRSGQADVWDVLTRRFESRLIRTALQATHG
RRMEAAQRLGIGRNTITRKIQELGLDAHDDE

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=505, Percent_Identity=56.039603960396, Blast_Score=535, Evalue=1e-153,
Organism=Escherichia coli, GI1788550, Length=512, Percent_Identity=38.4765625, Blast_Score=354, Evalue=7e-99,
Organism=Escherichia coli, GI1790437, Length=401, Percent_Identity=39.4014962593516, Blast_Score=286, Evalue=3e-78,
Organism=Escherichia coli, GI1788905, Length=326, Percent_Identity=43.558282208589, Blast_Score=268, Evalue=9e-73,
Organism=Escherichia coli, GI87082117, Length=243, Percent_Identity=49.7942386831276, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1789233, Length=367, Percent_Identity=38.1471389645777, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1789087, Length=367, Percent_Identity=38.6920980926431, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082152, Length=263, Percent_Identity=46.3878326996198, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1787583, Length=399, Percent_Identity=35.0877192982456, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786524, Length=290, Percent_Identity=43.7931034482759, Blast_Score=208, Evalue=6e-55,
Organism=Escherichia coli, GI87081872, Length=226, Percent_Identity=43.3628318584071, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789828, Length=209, Percent_Identity=38.2775119617225, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87081858, Length=346, Percent_Identity=28.0346820809249, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55709; Mature: 55709

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTQARDVLDALADVAAGDPTHQGPQVLVSD
CCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
IRMPGGSGLQLLEKVRGLQPGLPVIVMTAYSDLDSAVSAFQGGAFEYLPKPFDLPKAVEL
HCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHH
IRRAVEESQREEVAEQQLTAMPEMLGQAPAMQDVFRAIGRLSQSVVTVLITGESGSGKEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHH
VARALHKHSPVAGGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
HHHHHHHCCCCCCCCEEEEECHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEE
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKANVRVIAATHQNLEQRVKDGAFRED
EHHHHCCCCHHHHHHHHHHHCCCCEEEECCCEEEEECEEEEEHHHHHHHHHHHCCHHHHH
LFHRLNVIRLRLPALRERSEDVAMLTRYFLQRSARQLGVEPKRISDAALALLTRFAFPGN
HHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
VRQLENICHWLTVMAPAQVISAQDLPPEVLEATPHPAAPLQGVADEVAAGATSVPAPALP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
PAPAGTRTDQAAALDGMGWEPALVSEAQQLLRSGQADVWDVLTRRFESRLIRTALQATHG
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RRMEAAQRLGIGRNTITRKIQELGLDAHDDE
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKPIWIVDDDPSIRFVLEKALARENLPTRSFTQARDVLDALADVAAGDPTHQGPQVLVSD
CCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
IRMPGGSGLQLLEKVRGLQPGLPVIVMTAYSDLDSAVSAFQGGAFEYLPKPFDLPKAVEL
HCCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHH
IRRAVEESQREEVAEQQLTAMPEMLGQAPAMQDVFRAIGRLSQSVVTVLITGESGSGKEL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHH
VARALHKHSPVAGGPFVAINTAAIPKDLLESELFGHERGAFTGAQTQRRGRFEQAEGGTL
HHHHHHHCCCCCCCCEEEEECHHCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCEE
FLDEIGDMPFDLQTRLLRVLSDGQFYRVGGHAAVKANVRVIAATHQNLEQRVKDGAFRED
EHHHHCCCCHHHHHHHHHHHCCCCEEEECCCEEEEECEEEEEHHHHHHHHHHHCCHHHHH
LFHRLNVIRLRLPALRERSEDVAMLTRYFLQRSARQLGVEPKRISDAALALLTRFAFPGN
HHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
VRQLENICHWLTVMAPAQVISAQDLPPEVLEATPHPAAPLQGVADEVAAGATSVPAPALP
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCC
PAPAGTRTDQAAALDGMGWEPALVSEAQQLLRSGQADVWDVLTRRFESRLIRTALQATHG
CCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RRMEAAQRLGIGRNTITRKIQELGLDAHDDE
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]