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The map label for this gene is ntrB [H]

Identifier: 222110324

GI number: 222110324

Start: 1164357

End: 1165445

Strand: Direct

Name: ntrB [H]

Synonym: Dtpsy_1111

Alternate gene names: 222110324

Gene position: 1164357-1165445 (Clockwise)

Preceding gene: 222110323

Following gene: 222110325

Centisome position: 30.67

GC content: 68.41

Gene sequence:

>1089_bases
GTGCGGCACAAAGGCGCGATCATCCAAGAGCCCACCCCAACGGACCGCTACCAGGCGCTGGACCTCGTCTCCACGCTGAT
CGCCGTGCTGCACCCCGGCGGGGCGGCCCTCTTCGTCAACGCAGCGCTGGAAAACGCCCTGGGCCTGTCGCGCCGCACGC
TGGAAGGCGTGGACTTCGCCGGCCTGTTCACCGATCCGACCATCCTGCAGACGGCGCTGGCCGGAGCGCGCGGGCGCGAC
TTCGCGGCGCTGCGCTTCGAAGCCGTGCTGCGCCGCTCGCCGCAGGAGCCTTTGCCAGTGCACGCCAGCGTGGCGGTGGT
GGAGCACAGCGGCGACGTGATCGTGGAGCTGTGGCCCCTGGAGGCGCAGGCGCGCCAGGACCGCGAGGAGCGGCTGCGCG
AGCAGGCGCAGGCCAACAAGGAGCTGATCCGCAATTTGGCGCACGAGATCAAGAACCCGCTGGGCGGCATCCGAGGGGCG
GCGCAACTGCTGGAGATGGAGCTGGAAAGCCGCGACCTGACCGAGTACACCCAGGTCATCATCCACGAGGCCGACCGCCT
GCAAAGCCTGGTGGACCGGCTGCTGGCGCCGCACCGTCACCCGCACCTCGTCGGCGATGTGAATATCCACGAGGTCTGCG
AGCGCGTGCGCTCCCTGGTATTGGTCGAGCATCCGCAGGGCCTGGCCGTGCTGCGCGACTATGACACCTCCATCCCTGAG
TTTCGCGGCGACCGCGCGCAGCTCATCCAGGCCGTGCTGAACATCGTGCAGAACGCGGCGCAGGCGTTGCACGAGCGCAT
TGCCGCGGGCGACGCGGTCATCACGCTGCGCACGCGCGTGGCGCGGCAGGTCACGTTCGGGCGCCAACGCTACCGGCTGG
CATTGGAATTGCATGTCATCGACAACGGACCGGGCGTGCCCGAAGCCATCAAGGAGCGGATCTTCTATCCGCTGGTGACG
GGGCGGGACGGGGGATCGGGACTGGGCTTGACGCTGGCGCAAACCTTTGTGCAGCGACACCATGGGCTCATTGAGTGCGA
GAGCCAGCCGGGCCGCACCGACTTCCGCATCCTCATACCACTGCCTTGA

Upstream 100 bases:

>100_bases
ACGGCCGAGCTCAAGGCCAGCGTGGCCCGGGCCGAGGCCGATGTGGCCGGCATCAAGCGCGAACTCTCGCGCCTGAACAA
GTAAGCGCCGCGGTCCCTGC

Downstream 100 bases:

>100_bases
GTCCCTGACACGCACGACCCCAACGGGTAAAGGTAAAGGGAGCTGGAACACATGAAGCCGATCTGGATAGTAGACGACGA
CCCCTCGATCCGCTTCGTCC

Product: signal transduction histidine kinase, nitrogen specific, ntrb

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRHKGAIIQEPTPTDRYQALDLVSTLIAVLHPGGAALFVNAALENALGLSRRTLEGVDFAGLFTDPTILQTALAGARGRD
FAALRFEAVLRRSPQEPLPVHASVAVVEHSGDVIVELWPLEAQARQDREERLREQAQANKELIRNLAHEIKNPLGGIRGA
AQLLEMELESRDLTEYTQVIIHEADRLQSLVDRLLAPHRHPHLVGDVNIHEVCERVRSLVLVEHPQGLAVLRDYDTSIPE
FRGDRAQLIQAVLNIVQNAAQALHERIAAGDAVITLRTRVARQVTFGRQRYRLALELHVIDNGPGVPEAIKERIFYPLVT
GRDGGSGLGLTLAQTFVQRHHGLIECESQPGRTDFRILIPLP

Sequences:

>Translated_362_residues
MRHKGAIIQEPTPTDRYQALDLVSTLIAVLHPGGAALFVNAALENALGLSRRTLEGVDFAGLFTDPTILQTALAGARGRD
FAALRFEAVLRRSPQEPLPVHASVAVVEHSGDVIVELWPLEAQARQDREERLREQAQANKELIRNLAHEIKNPLGGIRGA
AQLLEMELESRDLTEYTQVIIHEADRLQSLVDRLLAPHRHPHLVGDVNIHEVCERVRSLVLVEHPQGLAVLRDYDTSIPE
FRGDRAQLIQAVLNIVQNAAQALHERIAAGDAVITLRTRVARQVTFGRQRYRLALELHVIDNGPGVPEAIKERIFYPLVT
GRDGGSGLGLTLAQTFVQRHHGLIECESQPGRTDFRILIPLP
>Mature_362_residues
MRHKGAIIQEPTPTDRYQALDLVSTLIAVLHPGGAALFVNAALENALGLSRRTLEGVDFAGLFTDPTILQTALAGARGRD
FAALRFEAVLRRSPQEPLPVHASVAVVEHSGDVIVELWPLEAQARQDREERLREQAQANKELIRNLAHEIKNPLGGIRGA
AQLLEMELESRDLTEYTQVIIHEADRLQSLVDRLLAPHRHPHLVGDVNIHEVCERVRSLVLVEHPQGLAVLRDYDTSIPE
FRGDRAQLIQAVLNIVQNAAQALHERIAAGDAVITLRTRVARQVTFGRQRYRLALELHVIDNGPGVPEAIKERIFYPLVT
GRDGGSGLGLTLAQTFVQRHHGLIECESQPGRTDFRILIPLP

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=251, Percent_Identity=47.808764940239, Blast_Score=225, Evalue=3e-60,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=29.7520661157025, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=29.6296296296296, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790346, Length=237, Percent_Identity=28.2700421940928, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789149, Length=240, Percent_Identity=25.4166666666667, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 40132; Mature: 40132

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHKGAIIQEPTPTDRYQALDLVSTLIAVLHPGGAALFVNAALENALGLSRRTLEGVDFA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHCCCHHHHCCCCEE
GLFTDPTILQTALAGARGRDFAALRFEAVLRRSPQEPLPVHASVAVVEHSGDVIVELWPL
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECC
EAQARQDREERLREQAQANKELIRNLAHEIKNPLGGIRGAAQLLEMELESRDLTEYTQVI
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IHEADRLQSLVDRLLAPHRHPHLVGDVNIHEVCERVRSLVLVEHPQGLAVLRDYDTSIPE
HHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHEEEECCCCCEEEECCCCCCCC
FRGDRAQLIQAVLNIVQNAAQALHERIAAGDAVITLRTRVARQVTFGRQRYRLALELHVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCHHHEEEEEEEEEE
DNGPGVPEAIKERIFYPLVTGRDGGSGLGLTLAQTFVQRHHGLIECESQPGRTDFRILIP
CCCCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEE
LP
CC
>Mature Secondary Structure
MRHKGAIIQEPTPTDRYQALDLVSTLIAVLHPGGAALFVNAALENALGLSRRTLEGVDFA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHEEHHHHHHHHHCCCHHHHCCCCEE
GLFTDPTILQTALAGARGRDFAALRFEAVLRRSPQEPLPVHASVAVVEHSGDVIVELWPL
CCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCEEEEEECC
EAQARQDREERLREQAQANKELIRNLAHEIKNPLGGIRGAAQLLEMELESRDLTEYTQVI
CHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
IHEADRLQSLVDRLLAPHRHPHLVGDVNIHEVCERVRSLVLVEHPQGLAVLRDYDTSIPE
HHHHHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHEEEECCCCCEEEECCCCCCCC
FRGDRAQLIQAVLNIVQNAAQALHERIAAGDAVITLRTRVARQVTFGRQRYRLALELHVI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCHHHEEEEEEEEEE
DNGPGVPEAIKERIFYPLVTGRDGGSGLGLTLAQTFVQRHHGLIECESQPGRTDFRILIP
CCCCCCHHHHHHHHHEEEEECCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEE
LP
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]