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The map label for this gene is glnA [H]

Identifier: 222110322

GI number: 222110322

Start: 1162214

End: 1163629

Strand: Direct

Name: glnA [H]

Synonym: Dtpsy_1109

Alternate gene names: 222110322

Gene position: 1162214-1163629 (Clockwise)

Preceding gene: 222110321

Following gene: 222110323

Centisome position: 30.61

GC content: 63.56

Gene sequence:

>1416_bases
ATGGCCAAGACCGTTGCAGACGTGATGAAGATGGTGAAGGAAAACGAAGTCAAGTTCGTGGACTTCCGCTTCACGGATAC
CCGCGGCAAGGAACAGCACGTCACCGTGCCGATCTCGCACTTCGACGAAGACAAGTTCGCCTCGGGCCATGCGTTCGACG
GCTCGTCGGTCGCCGGCTGGAAGGGTATCGAGGCGTCGGACATGCTACTCATGCCCGATCCGAACACCGCCAACATCGAC
CCGTTCTTCGAAGAAACCACCATGATCATGGCGTGCGACGTGGTGGAGCCGGCCGACGGCAAGGCCTACGACCGCGACCC
GCGCTCCATCGCCAAGCGCGCTGAGGCGTACTTGAAGGCCTCCGGCATGGGCGACACGGCCTACTTCGGCCCGGAGCCCG
AGTTCTTCATCTTCGACGGCGTGCGCTGGAGCACCGATCCCAACCACACCTTCTACGAGATCGAAGAGTACGAAGCCCCC
TGGAACACCGGCTCCAAGCTCGAAGGTGGCAACCGCGGCCACCGTCCCACCACCAAGGGCGGCTATTTCCCCGTGCCTCC
GGTCGACAGCACGCAGGACATGCGCGCAGAGATGTCCCTGATCCTTGAATCCCTGGGCATTCCGGTCGAGGTGTTCCACC
ACGAAGTGGCCGGTGCCGGCCAGAACGAGATCGGCACCCGGTTCAGCACGCTGGTGGAGCGTGCCGACTGGACCATGCTG
CAGAAGTACGTGATCCACAACGTGGCCAATGCCTACGGCAAGACCGCCACCTTCATGCCCAAGCCCTACCACGGCGACAA
CGGCTCGGGCATGCACGTGCACCAGTCGGTCTGGAAGGACGGCAAGAACCTGTTCGCCGGCGACGGCTATGCGGGTCTGT
CGGACTTCGCGCTGTACTACATCGGCGGCATCATTAAGCACGCCCGCGCACTGAACGCCATCACGAACCCGGGCACCAAC
AGCTACAAGCGTCTCGTGCCGCACTTCGAGGCGCCGGTGAAGCTGGCCTACTCGGCCAAGAACCGCTCGGCCTCCATCCG
CATCCCGTTCGTGGCCAACCCGAAGGGCCGCCGCGTGGAAGCGCGCTTCCCCGATCCGCTGATGAACCCCTACCTGGGCT
TTGCGGCCCTGCTGATGGCGGGTCTGGACGGCGTGGAAAACAAGATCCACCCGGGCGAAGCCGCTACCAAGGACCTGTAC
CACCTGCCGCCGGAGGAAGACAAGCTGGTGCCCACCGTGTGCCACAGCCTGGACCAGGCGCTGGAAGCGCTGGACAAGGA
CCGCGCGTTCCTGACCAAGGGCGGCGTGTTCACCGACTCCATGCTCGACGCCTACATCGAGCTGAAGATGCAGGAAGTCA
CGCGCTTCCGCATGGCCGTGCATCCCGTCGAGTACGACATGTACTACTCGCTGTGA

Upstream 100 bases:

>100_bases
GCCCGGCTTGCCCCGTACGGGTGTCCCCATTCGCTGGCACGCAAACTGCTTTTCTGTGCGCATCATTGTTGATGAGCACT
TCTATCCCTGGAGAACCCTG

Downstream 100 bases:

>100_bases
TGGCAGGGGCTTAGGCCCCCGGCTGTTGTCAACAGGGCGGCTCCGGCCGCCTTTTTCTATGGATGGGCTGCGCGCGTTGG
CTAACGGGGCACGGCATCCC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPISHFDEDKFASGHAFDGSSVAGWKGIEASDMLLMPDPNTANID
PFFEETTMIMACDVVEPADGKAYDRDPRSIAKRAEAYLKASGMGDTAYFGPEPEFFIFDGVRWSTDPNHTFYEIEEYEAP
WNTGSKLEGGNRGHRPTTKGGYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVERADWTML
QKYVIHNVANAYGKTATFMPKPYHGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTN
SYKRLVPHFEAPVKLAYSAKNRSASIRIPFVANPKGRRVEARFPDPLMNPYLGFAALLMAGLDGVENKIHPGEAATKDLY
HLPPEEDKLVPTVCHSLDQALEALDKDRAFLTKGGVFTDSMLDAYIELKMQEVTRFRMAVHPVEYDMYYSL

Sequences:

>Translated_471_residues
MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPISHFDEDKFASGHAFDGSSVAGWKGIEASDMLLMPDPNTANID
PFFEETTMIMACDVVEPADGKAYDRDPRSIAKRAEAYLKASGMGDTAYFGPEPEFFIFDGVRWSTDPNHTFYEIEEYEAP
WNTGSKLEGGNRGHRPTTKGGYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVERADWTML
QKYVIHNVANAYGKTATFMPKPYHGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTN
SYKRLVPHFEAPVKLAYSAKNRSASIRIPFVANPKGRRVEARFPDPLMNPYLGFAALLMAGLDGVENKIHPGEAATKDLY
HLPPEEDKLVPTVCHSLDQALEALDKDRAFLTKGGVFTDSMLDAYIELKMQEVTRFRMAVHPVEYDMYYSL
>Mature_470_residues
AKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPISHFDEDKFASGHAFDGSSVAGWKGIEASDMLLMPDPNTANIDP
FFEETTMIMACDVVEPADGKAYDRDPRSIAKRAEAYLKASGMGDTAYFGPEPEFFIFDGVRWSTDPNHTFYEIEEYEAPW
NTGSKLEGGNRGHRPTTKGGYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVERADWTMLQ
KYVIHNVANAYGKTATFMPKPYHGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTNS
YKRLVPHFEAPVKLAYSAKNRSASIRIPFVANPKGRRVEARFPDPLMNPYLGFAALLMAGLDGVENKIHPGEAATKDLYH
LPPEEDKLVPTVCHSLDQALEALDKDRAFLTKGGVFTDSMLDAYIELKMQEVTRFRMAVHPVEYDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=464, Percent_Identity=63.1465517241379, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=390, Percent_Identity=25.1282051282051, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52448; Mature: 52317

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPISHFDEDKFASGHAFDGSSVAGW
CCCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPNTANIDPFFEETTMIMACDVVEPADGKAYDRDPRSIAKRAEAYLKA
CCCCCCCEEEECCCCCCCCCCCHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
SGMGDTAYFGPEPEFFIFDGVRWSTDPNHTFYEIEEYEAPWNTGSKLEGGNRGHRPTTKG
CCCCCCCEECCCCCEEEECCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVERADWTML
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QKYVIHNVANAYGKTATFMPKPYHGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSDFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPHFEAPVKLAYSAKNRSASIRIPFVANPKGRRVE
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEE
ARFPDPLMNPYLGFAALLMAGLDGVENKIHPGEAATKDLYHLPPEEDKLVPTVCHSLDQA
CCCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
LEALDKDRAFLTKGGVFTDSMLDAYIELKMQEVTRFRMAVHPVEYDMYYSL
HHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure 
AKTVADVMKMVKENEVKFVDFRFTDTRGKEQHVTVPISHFDEDKFASGHAFDGSSVAGW
CCHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEEEEHHHCCCCCCCCCCCCCCCCCCCC
KGIEASDMLLMPDPNTANIDPFFEETTMIMACDVVEPADGKAYDRDPRSIAKRAEAYLKA
CCCCCCCEEEECCCCCCCCCCCHHCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
SGMGDTAYFGPEPEFFIFDGVRWSTDPNHTFYEIEEYEAPWNTGSKLEGGNRGHRPTTKG
CCCCCCCEECCCCCEEEECCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAGAGQNEIGTRFSTLVERADWTML
CCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QKYVIHNVANAYGKTATFMPKPYHGDNGSGMHVHQSVWKDGKNLFAGDGYAGLSDFALYY
HHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEHHHHHHCCCCEECCCCCCCHHHHHHHH
IGGIIKHARALNAITNPGTNSYKRLVPHFEAPVKLAYSAKNRSASIRIPFVANPKGRRVE
HHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEECCCCCEEE
ARFPDPLMNPYLGFAALLMAGLDGVENKIHPGEAATKDLYHLPPEEDKLVPTVCHSLDQA
CCCCCHHCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHH
LEALDKDRAFLTKGGVFTDSMLDAYIELKMQEVTRFRMAVHPVEYDMYYSL
HHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]