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The map label for this gene is hisS

Identifier: 222110304

GI number: 222110304

Start: 1138984

End: 1140273

Strand: Direct

Name: hisS

Synonym: Dtpsy_1091

Alternate gene names: 222110304

Gene position: 1138984-1140273 (Clockwise)

Preceding gene: 222110303

Following gene: 222110305

Centisome position: 30.0

GC content: 66.51

Gene sequence:

>1290_bases
ATGCAGAAGCTGGTTGCCATCAAGGGCATGAACGACATTCTTCCGCCCGATTCGGCGCGCTGGGAATGGCTGGAGGACAA
GGTGCGCACCCTCATGGCGCGCTACGCTTATCGCAACATCCGCACGCCCATCGTCGAGCCTACGCCGCTGTTCGTGCGCG
GTCTGGGCGAAGTCACCGACATCGTCGAGAAGGAGATGTACTCCTTCGAGGACCGGCTCAACGGTGAACAGCTGACGCTG
CGCCCCGAGGCAACGGCGGGGGTGGTGCGTGCGGTGGTAGAGCATTCCATGCTCTACGACGGCGGCAAGCGCCTGTACTA
CATGGGGCCCATGTTCCGGCATGAACGGCCACAGCGCGGCCGCTACCGCCAGTTCCACCAGATCGGTGCCGAGGCGCTGG
GCTTTCCCGGCGCGGAAGCCGATGCCGAGATCATTCTGCTGGCGCATGCGCTGTGGGCGGAACTGGGGCTGGAGAACGTG
CGGCTGGAATTGAACAGCCTGGGGCAGCCCGACGAGCGCCGCGCCCACCGCGCGGCGCTGATCGCATACCTGGAGCAGCA
CATGGATGTGCTCGACGAGGATGCGCGCCGCCGCCTGCACAGCAATCCGCTGCGCATTCTCGACACCAAGAACCCCGCGA
TGCAGGCGCTGGTGGAAGGCGCCCCGCGGCTCATCGACTTTCTAGGCGAGGCGTCGCTGCAGCATTTCGAGACCGTCAAG
GCGATCCTGGATGCCAACGGCGTGGCCTGGAGCCTCAACCCCCGCCTGGTCCGCGGGATGGACTATTACAACCTCACGGT
ATTCGAGTTCGTGACGGACCAACTGGGTTCGCAGGGCACGATCTGTGGTGGTGGCCGTTACGACTACCTGATCGAGCAGA
TCGGCGGCAAGTTCGCTCCGGCCGTGGGCTGGGCGCTGGGGGTGGAGCGCGTGCTGGAGTTGCTCAAGGAGCAGGAGACG
CAAGTGGCCCGACCAGCTGCCGATGCCTATGCCGTAGTGCCCGACGCGTCTGCCCTGCCGGTGGTCATGGCTGCCTTGCA
GCGGCTGCGTGCGCAGGGGGTGTCGGTGCAGATGCACAGCCCCACCGCCGCAGGCGAGGGCATGGGCAGCATGAAATCCC
AGTTCAAGAAGGCGGACGCCAGCGGCGCACGCTATGCACTGGTCTTCGGCGCCGACGAATTGGCCCGCGGGGCCGTGACC
GTCAAGCCGCTGCGGGATAGCGGCGAGCAGGCGGAGCGCCCGCTGGCGGCGCTGGCCGAATGGGCGGCCACCCTACAATC
CTCGCGCTGA

Upstream 100 bases:

>100_bases
TGAAGTGTGCGCAAGTTTGCTATAAATATTATAGCTTATAGCGCTTGATGGATAAGCGCTAGAGGCTGATTTGATTCAAA
ATCACAAACAATAGAACAAG

Downstream 100 bases:

>100_bases
ACGGAACCACAACATGGCCAACAACTTCGACCTTCAAGAACAAGAACAGCTTGAGCAGATCAAGCACTTCTGGAACACGT
GGGGCACGCCCATCACCTGG

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MQKLVAIKGMNDILPPDSARWEWLEDKVRTLMARYAYRNIRTPIVEPTPLFVRGLGEVTDIVEKEMYSFEDRLNGEQLTL
RPEATAGVVRAVVEHSMLYDGGKRLYYMGPMFRHERPQRGRYRQFHQIGAEALGFPGAEADAEIILLAHALWAELGLENV
RLELNSLGQPDERRAHRAALIAYLEQHMDVLDEDARRRLHSNPLRILDTKNPAMQALVEGAPRLIDFLGEASLQHFETVK
AILDANGVAWSLNPRLVRGMDYYNLTVFEFVTDQLGSQGTICGGGRYDYLIEQIGGKFAPAVGWALGVERVLELLKEQET
QVARPAADAYAVVPDASALPVVMAALQRLRAQGVSVQMHSPTAAGEGMGSMKSQFKKADASGARYALVFGADELARGAVT
VKPLRDSGEQAERPLAALAEWAATLQSSR

Sequences:

>Translated_429_residues
MQKLVAIKGMNDILPPDSARWEWLEDKVRTLMARYAYRNIRTPIVEPTPLFVRGLGEVTDIVEKEMYSFEDRLNGEQLTL
RPEATAGVVRAVVEHSMLYDGGKRLYYMGPMFRHERPQRGRYRQFHQIGAEALGFPGAEADAEIILLAHALWAELGLENV
RLELNSLGQPDERRAHRAALIAYLEQHMDVLDEDARRRLHSNPLRILDTKNPAMQALVEGAPRLIDFLGEASLQHFETVK
AILDANGVAWSLNPRLVRGMDYYNLTVFEFVTDQLGSQGTICGGGRYDYLIEQIGGKFAPAVGWALGVERVLELLKEQET
QVARPAADAYAVVPDASALPVVMAALQRLRAQGVSVQMHSPTAAGEGMGSMKSQFKKADASGARYALVFGADELARGAVT
VKPLRDSGEQAERPLAALAEWAATLQSSR
>Mature_429_residues
MQKLVAIKGMNDILPPDSARWEWLEDKVRTLMARYAYRNIRTPIVEPTPLFVRGLGEVTDIVEKEMYSFEDRLNGEQLTL
RPEATAGVVRAVVEHSMLYDGGKRLYYMGPMFRHERPQRGRYRQFHQIGAEALGFPGAEADAEIILLAHALWAELGLENV
RLELNSLGQPDERRAHRAALIAYLEQHMDVLDEDARRRLHSNPLRILDTKNPAMQALVEGAPRLIDFLGEASLQHFETVK
AILDANGVAWSLNPRLVRGMDYYNLTVFEFVTDQLGSQGTICGGGRYDYLIEQIGGKFAPAVGWALGVERVLELLKEQET
QVARPAADAYAVVPDASALPVVMAALQRLRAQGVSVQMHSPTAAGEGMGSMKSQFKKADASGARYALVFGADELARGAVT
VKPLRDSGEQAERPLAALAEWAATLQSSR

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=412, Percent_Identity=52.1844660194175, Blast_Score=432, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_ACIET (B9MFX7)

Other databases:

- EMBL:   CP001392
- RefSeq:   YP_002552568.1
- GeneID:   7382900
- GenomeReviews:   CP001392_GR
- KEGG:   dia:Dtpsy_1091
- ProtClustDB:   PRK00037
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47533; Mature: 47533

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLVAIKGMNDILPPDSARWEWLEDKVRTLMARYAYRNIRTPIVEPTPLFVRGLGEVTD
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IVEKEMYSFEDRLNGEQLTLRPEATAGVVRAVVEHSMLYDGGKRLYYMGPMFRHERPQRG
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCH
RYRQFHQIGAEALGFPGAEADAEIILLAHALWAELGLENVRLELNSLGQPDERRAHRAAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHEEEHHCCCCCHHHHHHHHHH
IAYLEQHMDVLDEDARRRLHSNPLRILDTKNPAMQALVEGAPRLIDFLGEASLQHFETVK
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AILDANGVAWSLNPRLVRGMDYYNLTVFEFVTDQLGSQGTICGGGRYDYLIEQIGGKFAP
HHHCCCCCEEECCCHHHCCCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCCHHH
AVGWALGVERVLELLKEQETQVARPAADAYAVVPDASALPVVMAALQRLRAQGVSVQMHS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECC
PTAAGEGMGSMKSQFKKADASGARYALVFGADELARGAVTVKPLRDSGEQAERPLAALAE
CCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHCCCEEEEECCCCCHHHHHHHHHHHH
WAATLQSSR
HHHHHHCCC
>Mature Secondary Structure
MQKLVAIKGMNDILPPDSARWEWLEDKVRTLMARYAYRNIRTPIVEPTPLFVRGLGEVTD
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
IVEKEMYSFEDRLNGEQLTLRPEATAGVVRAVVEHSMLYDGGKRLYYMGPMFRHERPQRG
HHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCCH
RYRQFHQIGAEALGFPGAEADAEIILLAHALWAELGLENVRLELNSLGQPDERRAHRAAL
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHEEEHHCCCCCHHHHHHHHHH
IAYLEQHMDVLDEDARRRLHSNPLRILDTKNPAMQALVEGAPRLIDFLGEASLQHFETVK
HHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
AILDANGVAWSLNPRLVRGMDYYNLTVFEFVTDQLGSQGTICGGGRYDYLIEQIGGKFAP
HHHCCCCCEEECCCHHHCCCCCHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHCCCHHH
AVGWALGVERVLELLKEQETQVARPAADAYAVVPDASALPVVMAALQRLRAQGVSVQMHS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCEEEECC
PTAAGEGMGSMKSQFKKADASGARYALVFGADELARGAVTVKPLRDSGEQAERPLAALAE
CCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHHCCCEEEEECCCCCHHHHHHHHHHHH
WAATLQSSR
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA