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The map label for this gene is ispG [H]

Identifier: 222110303

GI number: 222110303

Start: 1137609

End: 1138886

Strand: Direct

Name: ispG [H]

Synonym: Dtpsy_1090

Alternate gene names: 222110303

Gene position: 1137609-1138886 (Clockwise)

Preceding gene: 222110302

Following gene: 222110304

Centisome position: 29.96

GC content: 65.88

Gene sequence:

>1278_bases
ATGAATATCTCCGGCACAGCCGCACCGATCGCTATGGCACAGCCCCAGGCACGCCGCTCGCGGCAGGCACGCATCGTCTG
GGGCAGCCGGGTGGTCACGGTGGGTGGCGGCGCACCGGTGCGGGTGCAGTCCATGACCAACACCGACACGGTGGATGCGA
TCGGCACGGCCATCCAGATCAAGGAACTGGCGCAAGCGGGCTCTGAGTTCGTTCGCATCACGGTGAACACGCCGGAGGCC
GCAGCGGCCGTGCCCTACATCCGTGAGCAGCTGGACCGCATGGGCGAGACGGTGCCGCTGGTGGGCGACTTTCACTACAA
CGGCCACCGCCTGCTGACGGAATTTCCCGACTGCGCACAGGCATTGTCCAAGTACCGCATCAACCCGGGCAACGTGGGCA
AGGGCGACAAGAAGGACAAGCAGTTCGGCCAGATGATCGAGGCCGCGGTGCGCTGGGACAAGGCCGTGCGCATCGGCGTG
AACTGGGGCAGCCTGGACCAGGAGTTGCTGGCCGAGCTGATGGATGCGAACAGCCGCCGCGCCCAGCCGTGGGATGCGCG
CCAGGTCATGTACGAGGCGCTGATCACCTCCGCCATCGAATCGGCGAAACGTGCCGAGGAATTGGGCCTGAGCGGCGACC
AGATCATCCTTTCGTGCAAGGTCAGCGGTGTGCAGGATCTGATCTCGGTGTACCGCGAACTCGCGCGCCGCTGCGACTAT
GCGCTGCACCTGGGTCTGACCGAGGCGGGCATGGGTACCAAGGGTATCGTGGCGTCGACCACCGCGCTTTCGATCCTGCT
GCAGGAAGGCATTGGCGACACTATCCGTGTGTCGCTGACTCCGCAGCCCGGCGAGGCGCGCACCCAGGAGGTGGTGGTGG
CCTCCGAGATCCTCCAATCGCTGGGGCTGCGCGCGTTCGTGCCCAGCGTGACTGCTTGCCCGGGCTGCGGCCGTACCACC
AGCACCACATTCCAGGAGCTGGCCAAGCAGATCGACGATTACCTGCGTGCGCAGATGCCCGTATGGCGGCTGCGCTATCC
CGGCGTGGAAAAACTCAAGGTGGCCGTGATGGGCTGCATCGTCAACGGCCCGGGCGAGAGCAAACATGCCGACATCGGCA
TCAGCCTTCCCGGCACGGGCGAGGCGCCCGCCGCCCCGGTGTTCATCGACGGCGAAAAGGCCATGACCCTGCGGGGCGAG
GGCATCGCGCGAGAGTTCCAGGATATCGTTGAAGGCTACATTGCCCGGCGTTTCGGCAACGGCGCAGGCACACAGTGA

Upstream 100 bases:

>100_bases
CATTGGCAAGGCGGATGCCACGGAGGTGGTTGTGCGCGGCCAGCCCATGGATCTGAATGCGATTGCGCGCGAGAACGTTG
CGCGTTTCGAGGTGAAGTAA

Downstream 100 bases:

>100_bases
AGTGTGCGCAAGTTTGCTATAAATATTATAGCTTATAGCGCTTGATGGATAAGCGCTAGAGGCTGATTTGATTCAAAATC
ACAAACAATAGAACAAGATG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MNISGTAAPIAMAQPQARRSRQARIVWGSRVVTVGGGAPVRVQSMTNTDTVDAIGTAIQIKELAQAGSEFVRITVNTPEA
AAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEFPDCAQALSKYRINPGNVGKGDKKDKQFGQMIEAAVRWDKAVRIGV
NWGSLDQELLAELMDANSRRAQPWDARQVMYEALITSAIESAKRAEELGLSGDQIILSCKVSGVQDLISVYRELARRCDY
ALHLGLTEAGMGTKGIVASTTALSILLQEGIGDTIRVSLTPQPGEARTQEVVVASEILQSLGLRAFVPSVTACPGCGRTT
STTFQELAKQIDDYLRAQMPVWRLRYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGE
GIAREFQDIVEGYIARRFGNGAGTQ

Sequences:

>Translated_425_residues
MNISGTAAPIAMAQPQARRSRQARIVWGSRVVTVGGGAPVRVQSMTNTDTVDAIGTAIQIKELAQAGSEFVRITVNTPEA
AAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEFPDCAQALSKYRINPGNVGKGDKKDKQFGQMIEAAVRWDKAVRIGV
NWGSLDQELLAELMDANSRRAQPWDARQVMYEALITSAIESAKRAEELGLSGDQIILSCKVSGVQDLISVYRELARRCDY
ALHLGLTEAGMGTKGIVASTTALSILLQEGIGDTIRVSLTPQPGEARTQEVVVASEILQSLGLRAFVPSVTACPGCGRTT
STTFQELAKQIDDYLRAQMPVWRLRYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGE
GIAREFQDIVEGYIARRFGNGAGTQ
>Mature_425_residues
MNISGTAAPIAMAQPQARRSRQARIVWGSRVVTVGGGAPVRVQSMTNTDTVDAIGTAIQIKELAQAGSEFVRITVNTPEA
AAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEFPDCAQALSKYRINPGNVGKGDKKDKQFGQMIEAAVRWDKAVRIGV
NWGSLDQELLAELMDANSRRAQPWDARQVMYEALITSAIESAKRAEELGLSGDQIILSCKVSGVQDLISVYRELARRCDY
ALHLGLTEAGMGTKGIVASTTALSILLQEGIGDTIRVSLTPQPGEARTQEVVVASEILQSLGLRAFVPSVTACPGCGRTT
STTFQELAKQIDDYLRAQMPVWRLRYPGVEKLKVAVMGCIVNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGE
GIAREFQDIVEGYIARRFGNGAGTQ

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=369, Percent_Identity=34.6883468834688, Blast_Score=182, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 45737; Mature: 45737

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNISGTAAPIAMAQPQARRSRQARIVWGSRVVTVGGGAPVRVQSMTNTDTVDAIGTAIQI
CCCCCCCCCCCCCCCHHHHCCCEEEEECCEEEEECCCCCEEEEECCCCCHHHHHHHHHHH
KELAQAGSEFVRITVNTPEAAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEFPDCAQ
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHCCHHHHH
ALSKYRINPGNVGKGDKKDKQFGQMIEAAVRWDKAVRIGVNWGSLDQELLAELMDANSRR
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCCCCC
AQPWDARQVMYEALITSAIESAKRAEELGLSGDQIILSCKVSGVQDLISVYRELARRCDY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
ALHLGLTEAGMGTKGIVASTTALSILLQEGIGDTIRVSLTPQPGEARTQEVVVASEILQS
EEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
LGLRAFVPSVTACPGCGRTTSTTFQELAKQIDDYLRAQMPVWRLRYPGVEKLKVAVMGCI
CCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEE
VNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGEGIAREFQDIVEGYIARRFGN
ECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
GAGTQ
CCCCC
>Mature Secondary Structure
MNISGTAAPIAMAQPQARRSRQARIVWGSRVVTVGGGAPVRVQSMTNTDTVDAIGTAIQI
CCCCCCCCCCCCCCCHHHHCCCEEEEECCEEEEECCCCCEEEEECCCCCHHHHHHHHHHH
KELAQAGSEFVRITVNTPEAAAAVPYIREQLDRMGETVPLVGDFHYNGHRLLTEFPDCAQ
HHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHCCHHHHH
ALSKYRINPGNVGKGDKKDKQFGQMIEAAVRWDKAVRIGVNWGSLDQELLAELMDANSRR
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHCCCCCC
AQPWDARQVMYEALITSAIESAKRAEELGLSGDQIILSCKVSGVQDLISVYRELARRCDY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHCCC
ALHLGLTEAGMGTKGIVASTTALSILLQEGIGDTIRVSLTPQPGEARTQEVVVASEILQS
EEEECCHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHH
LGLRAFVPSVTACPGCGRTTSTTFQELAKQIDDYLRAQMPVWRLRYPGVEKLKVAVMGCI
CCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHEEEE
VNGPGESKHADIGISLPGTGEAPAAPVFIDGEKAMTLRGEGIAREFQDIVEGYIARRFGN
ECCCCCCCCCEEEEECCCCCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCC
GAGTQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA