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The map label for this gene is btuB [H]

Identifier: 222110226

GI number: 222110226

Start: 1052210

End: 1054090

Strand: Direct

Name: btuB [H]

Synonym: Dtpsy_1012

Alternate gene names: 222110226

Gene position: 1052210-1054090 (Clockwise)

Preceding gene: 222110223

Following gene: 222110227

Centisome position: 27.71

GC content: 67.36

Gene sequence:

>1881_bases
ATGACAAACACCCGTCCGTTCAATGTGCGCTTGAGTGCGCTTTCCGTGCACGCGCGCGTCGCCGTGCTGCCGCTGGCGCT
GGCCGCCGCTTTCCCCGCCGTGTCCCAGACCCTGCTGGCCCAGGCAGCCACAGAACCCCAACTGCGTGAAACCGTCATCA
CGGCAACCCGCGTGGCCCGGCCGCTGGCCGACATCGTGTCCGATGTGTCCGTCATCGACCGTGAAACTATCGAAGCCAGC
GGCGCGGTAGGCGTCATTGACGTGATCTCGCGCCTGCCAGGCGTGCAGATCACGCGCAATGGCGGCCAGGGCAGCAATGG
CAGCGTCTACCTGCGCGGCGCGGAGTCGCGCTTTACCGCCGTGTACCTGGACGGCGTGCGCCTGGACTCGCAATCCACCG
GCGGCGTGGTGTGGGAGCAAATCCCGTTGTCGCAGATCGACCGCATCGAGGTCGTGCGCGGGCCAGCGGCTGCTGTATAC
GGCTCGGATGCCATGGGAGGCGTGATCCAGCTCTTCACCAAAAAGGGCGAGGGTGCGCCGGCGCCCTACGTGGGCGTGGG
CCTGGGCAACCAGGGCACGCGGACGGTGCAGGCCGGCGTGAGTGGTGGCACCGAGCAGGTGGACTATGCGCTGGGGCTGT
CGCATGAGCGCGCCGATGGCTTCAGTGCCCGCGTAGGCTCCGCCTACAACCCGGACGAAGACGGCTACCGCCGCACCTCT
GCCAATGCCCGACTGGGCGTGCAGTTGGACACGACCCATCGCCTGGAGGGCACGCTGCTGGCCAGCAACCTCAACTCGCA
GTACGACGGCAGCAAAACGGCCGACGACCGCAATCACCACCAATTGCGCACGGCCAGCCTGGCATGGCTGGCCAAGTGGA
GCGACGTGTACAGCACGCGCTTGCAGGTCACGCAGGGGCAGAGTGAATACAGCACCCGGCCCAACTTCTACATGACCGAG
ACCACGCTGCACAATTACCTGCTGCAAAACGAGTGGCGCCTGGGCGCGCATCTGTTCACAGCCGCGCTGGAGCGCCGCGA
GGACGAGTTGCACAACCCCGCCACCGAGTTCGTGGGCGACCTCCAGCGTGACCGCTCACAGAACGCGCTCGCGCTGGGCT
ATGGCCTGAACCAGGGCGCCCATACGCTGCAGGCCAACCTGCGCTATGACGACGACAGCGAGTTCGGCGGCAAAAGCACG
GGCAGCCTGTCCTATGGCTACGCCATTACGCCGCAGTGGCGCGTCACCGCAGCGGCCGGCACGTCCTTCCGTGCACCCAC
GCTCTACCACCGCTTCAGCGAATACGGCGCGCCCGACCTGCAGCCCGAGAAGGGGCGCAACGTGGAGCTGGGCGCGCGGT
GGACGCAAGGAGAGAGCAGCGTGGGTGTCGTGGCGTACCGCAACCGCGTCAGCAACCTCATCAATTTCGGCGCCGCGGGC
CGCTGTGATTCCAGCTTCGGCTGCTACGAGAACGTGGGCCGCGCCGAGTACAAGGGCGTGACGCTGTCGGCCGCACACCG
CATCGCGGGCGTGTCGCTGCATGGCTCCATCGACTGGCAGAACCCGCGTGACCTGGACAGCGGCAAACTGCTGGCGCGCC
GTGCCAAGCGCTACGCCACGCTGGCTGCCGACACCCTGGTGGCAGGCTGGACGCTGGGGGCAGAACTCTACGCATCCGCG
CGCCGGTACGACAACGCGGCCAACACCAACGTACTGGGCGGCTACACCACGCTCAACCTCTACACCAGCAAGCGCGTGGC
GCGCGACGTGACGCTGCTGGCCCGCGTCGACAACCTGGCCGACAAGGACTACCAGTTGGCGCGCACCTACGCCACCGGCG
GCCGCACGTTCTACGTGGGCCTGAAGTGGGCGCCGCAGTAA

Upstream 100 bases:

>100_bases
GCCAACGAGGTGGCTCCGCGTTTCCTTTTGTGAAACCTGGGCCGTTATGCCCAGGTGCCGACGGGGAGGTCGGCCGTGGC
GATCTCTCTGTTTGCCCCAC

Downstream 100 bases:

>100_bases
CGCCATCACCGACTGCCACACCATGAGCCGCTGTCCCGCCCTGGTCATCGCCGCGCCCGCCTCTGGGCAGGGCAAGACCA
CCGTCACAGCCGCTTTGGCG

Product: tonb-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTNTRPFNVRLSALSVHARVAVLPLALAAAFPAVSQTLLAQAATEPQLRETVITATRVARPLADIVSDVSVIDRETIEAS
GAVGVIDVISRLPGVQITRNGGQGSNGSVYLRGAESRFTAVYLDGVRLDSQSTGGVVWEQIPLSQIDRIEVVRGPAAAVY
GSDAMGGVIQLFTKKGEGAPAPYVGVGLGNQGTRTVQAGVSGGTEQVDYALGLSHERADGFSARVGSAYNPDEDGYRRTS
ANARLGVQLDTTHRLEGTLLASNLNSQYDGSKTADDRNHHQLRTASLAWLAKWSDVYSTRLQVTQGQSEYSTRPNFYMTE
TTLHNYLLQNEWRLGAHLFTAALERREDELHNPATEFVGDLQRDRSQNALALGYGLNQGAHTLQANLRYDDDSEFGGKST
GSLSYGYAITPQWRVTAAAGTSFRAPTLYHRFSEYGAPDLQPEKGRNVELGARWTQGESSVGVVAYRNRVSNLINFGAAG
RCDSSFGCYENVGRAEYKGVTLSAAHRIAGVSLHGSIDWQNPRDLDSGKLLARRAKRYATLAADTLVAGWTLGAELYASA
RRYDNAANTNVLGGYTTLNLYTSKRVARDVTLLARVDNLADKDYQLARTYATGGRTFYVGLKWAPQ

Sequences:

>Translated_626_residues
MTNTRPFNVRLSALSVHARVAVLPLALAAAFPAVSQTLLAQAATEPQLRETVITATRVARPLADIVSDVSVIDRETIEAS
GAVGVIDVISRLPGVQITRNGGQGSNGSVYLRGAESRFTAVYLDGVRLDSQSTGGVVWEQIPLSQIDRIEVVRGPAAAVY
GSDAMGGVIQLFTKKGEGAPAPYVGVGLGNQGTRTVQAGVSGGTEQVDYALGLSHERADGFSARVGSAYNPDEDGYRRTS
ANARLGVQLDTTHRLEGTLLASNLNSQYDGSKTADDRNHHQLRTASLAWLAKWSDVYSTRLQVTQGQSEYSTRPNFYMTE
TTLHNYLLQNEWRLGAHLFTAALERREDELHNPATEFVGDLQRDRSQNALALGYGLNQGAHTLQANLRYDDDSEFGGKST
GSLSYGYAITPQWRVTAAAGTSFRAPTLYHRFSEYGAPDLQPEKGRNVELGARWTQGESSVGVVAYRNRVSNLINFGAAG
RCDSSFGCYENVGRAEYKGVTLSAAHRIAGVSLHGSIDWQNPRDLDSGKLLARRAKRYATLAADTLVAGWTLGAELYASA
RRYDNAANTNVLGGYTTLNLYTSKRVARDVTLLARVDNLADKDYQLARTYATGGRTFYVGLKWAPQ
>Mature_625_residues
TNTRPFNVRLSALSVHARVAVLPLALAAAFPAVSQTLLAQAATEPQLRETVITATRVARPLADIVSDVSVIDRETIEASG
AVGVIDVISRLPGVQITRNGGQGSNGSVYLRGAESRFTAVYLDGVRLDSQSTGGVVWEQIPLSQIDRIEVVRGPAAAVYG
SDAMGGVIQLFTKKGEGAPAPYVGVGLGNQGTRTVQAGVSGGTEQVDYALGLSHERADGFSARVGSAYNPDEDGYRRTSA
NARLGVQLDTTHRLEGTLLASNLNSQYDGSKTADDRNHHQLRTASLAWLAKWSDVYSTRLQVTQGQSEYSTRPNFYMTET
TLHNYLLQNEWRLGAHLFTAALERREDELHNPATEFVGDLQRDRSQNALALGYGLNQGAHTLQANLRYDDDSEFGGKSTG
SLSYGYAITPQWRVTAAAGTSFRAPTLYHRFSEYGAPDLQPEKGRNVELGARWTQGESSVGVVAYRNRVSNLINFGAAGR
CDSSFGCYENVGRAEYKGVTLSAAHRIAGVSLHGSIDWQNPRDLDSGKLLARRAKRYATLAADTLVAGWTLGAELYASAR
RYDNAANTNVLGGYTTLNLYTSKRVARDVTLLARVDNLADKDYQLARTYATGGRTFYVGLKWAPQ

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=621, Percent_Identity=27.6972624798712, Blast_Score=179, Evalue=5e-46,
Organism=Escherichia coli, GI1788478, Length=166, Percent_Identity=34.9397590361446, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1786798, Length=727, Percent_Identity=22.1458046767538, Blast_Score=74, Evalue=4e-14,
Organism=Escherichia coli, GI1787024, Length=184, Percent_Identity=27.1739130434783, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787723, Length=253, Percent_Identity=26.8774703557312, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786344, Length=176, Percent_Identity=26.7045454545455, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 67772; Mature: 67641

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTRPFNVRLSALSVHARVAVLPLALAAAFPAVSQTLLAQAATEPQLRETVITATRVAR
CCCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
PLADIVSDVSVIDRETIEASGAVGVIDVISRLPGVQITRNGGQGSNGSVYLRGAESRFTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEE
VYLDGVRLDSQSTGGVVWEQIPLSQIDRIEVVRGPAAAVYGSDAMGGVIQLFTKKGEGAP
EEEECEEECCCCCCCEEEECCCHHHCCEEEEECCCCEEEECCCCCCCHHHHHCCCCCCCC
APYVGVGLGNQGTRTVQAGVSGGTEQVDYALGLSHERADGFSARVGSAYNPDEDGYRRTS
CCEEEEECCCCCCEEEEECCCCCCHHHHHEECCCCCCCCCCHHCCCCCCCCCCCCCEECC
ANARLGVQLDTTHRLEGTLLASNLNSQYDGSKTADDRNHHQLRTASLAWLAKWSDVYSTR
CCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHE
LQVTQGQSEYSTRPNFYMTETTLHNYLLQNEWRLGAHLFTAALERREDELHNPATEFVGD
EEEECCCHHHCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LQRDRSQNALALGYGLNQGAHTLQANLRYDDDSEFGGKSTGSLSYGYAITPQWRVTAAAG
HHHCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECEEECCCEEEEEECC
TSFRAPTLYHRFSEYGAPDLQPEKGRNVELGARWTQGESSVGVVAYRNRVSNLINFGAAG
CCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCEEECCCCCEEEEEHHHHHHHHHHCCCCC
RCDSSFGCYENVGRAEYKGVTLSAAHRIAGVSLHGSIDWQNPRDLDSGKLLARRAKRYAT
CCCCCCCHHHCCCCHHCCCEEEEHHHHEEEEEEECEECCCCCCCCCCHHHHHHHHHHHHH
LAADTLVAGWTLGAELYASARRYDNAANTNVLGGYTTLNLYTSKRVARDVTLLARVDNLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEEEHHHHHHHHHHHHHHHHHC
DKDYQLARTYATGGRTFYVGLKWAPQ
CCCCEEEEEEECCCEEEEEEEEECCC
>Mature Secondary Structure 
TNTRPFNVRLSALSVHARVAVLPLALAAAFPAVSQTLLAQAATEPQLRETVITATRVAR
CCCCCEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
PLADIVSDVSVIDRETIEASGAVGVIDVISRLPGVQITRNGGQGSNGSVYLRGAESRFTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCEEE
VYLDGVRLDSQSTGGVVWEQIPLSQIDRIEVVRGPAAAVYGSDAMGGVIQLFTKKGEGAP
EEEECEEECCCCCCCEEEECCCHHHCCEEEEECCCCEEEECCCCCCCHHHHHCCCCCCCC
APYVGVGLGNQGTRTVQAGVSGGTEQVDYALGLSHERADGFSARVGSAYNPDEDGYRRTS
CCEEEEECCCCCCEEEEECCCCCCHHHHHEECCCCCCCCCCHHCCCCCCCCCCCCCEECC
ANARLGVQLDTTHRLEGTLLASNLNSQYDGSKTADDRNHHQLRTASLAWLAKWSDVYSTR
CCCEEEEEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHE
LQVTQGQSEYSTRPNFYMTETTLHNYLLQNEWRLGAHLFTAALERREDELHNPATEFVGD
EEEECCCHHHCCCCCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
LQRDRSQNALALGYGLNQGAHTLQANLRYDDDSEFGGKSTGSLSYGYAITPQWRVTAAAG
HHHCCCCCEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCCEEECEEECCCEEEEEECC
TSFRAPTLYHRFSEYGAPDLQPEKGRNVELGARWTQGESSVGVVAYRNRVSNLINFGAAG
CCCCCCHHHHHHHHCCCCCCCCCCCCCEEECCEEECCCCCEEEEEHHHHHHHHHHCCCCC
RCDSSFGCYENVGRAEYKGVTLSAAHRIAGVSLHGSIDWQNPRDLDSGKLLARRAKRYAT
CCCCCCCHHHCCCCHHCCCEEEEHHHHEEEEEEECEECCCCCCCCCCHHHHHHHHHHHHH
LAADTLVAGWTLGAELYASARRYDNAANTNVLGGYTTLNLYTSKRVARDVTLLARVDNLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCEEEEEEEEHHHHHHHHHHHHHHHHHC
DKDYQLARTYATGGRTFYVGLKWAPQ
CCCCEEEEEEECCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA