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The map label for this gene is oprM [H]

Identifier: 222110157

GI number: 222110157

Start: 969710

End: 971164

Strand: Direct

Name: oprM [H]

Synonym: Dtpsy_0943

Alternate gene names: 222110157

Gene position: 969710-971164 (Clockwise)

Preceding gene: 222110156

Following gene: 222110158

Centisome position: 25.54

GC content: 72.92

Gene sequence:

>1455_bases
ATGAAAAAGGAATTCCCCATGACGATGCTTTCCCTTGCTTTCCCCGCGCGACTGGCGCCGCTGGCCGCGGCCCTGGTGCT
GGCAGGCTGCATGGGCGCGCCGCTGGCGCCGCAGCCGGAACACGCGGGCGTTGCCGTGCCCGCCGCCTTCAGCGCAGCGG
CGACGGAGGGTGCACCGGCCTGGACGACCGCGCCGCCGGCGGAGGCACAGCCCCGCGGCGCCTGGTGGCTGGGTTTTCAG
GATCCGGTGCTGAGCGATCTGGTCGAACGCGCTGGTCAGGCCAATACCAGCGTGCAGGAGGCTGCGGCCCGCCTGGCCGA
GGCGCGCGCGCTGCTGCGCTCGGCAGATGCAGAGCGCGCACCCCAGGTGCGCGCCAACGCTGGCGTCTCACGCCAGGCCG
GTGTGACCACCACCAATGGCACGCAGCCTGCCACGCTCGCAACGGCGGGGCTGGGCGTGTCCTACGAGCTGGATCTGTTC
GGCCGGCTGGCGCGCGCCAGCGATGCGGCGCGGCTGGACGCCCAGGCCCGCGCCGCGCTGCTGCAGAGCACGCGGCTGAT
GGTGCAGGCCGACGTAGCGCAGACCTATTTGCAATTGCGTGCGCTGCAGGCGGAGCGCGCGCTGGTGGATGAGAGTCTGG
AAGCCTACCGCGGCACTCTGTACCTCACGCAGCGCCGCTACCAGGCGGGCGACGTGGCGGAGCTGGACGTGGCGCGCGTG
CAGACGGAGGTGGCGGCCACCGAATCCGAGGCACTGGCGCTGGAGCGCCAGCAGGCGCAGCTGCGGCATGCATTGGCCGT
GCTGGTGGGCGACGTGGCCACGGGCTTCTCGCTGCCACCGGCGGCGGTCGAGGCCGCGCTGCCGGTGATTCCGCCCGGCG
TGCCCAGCACGGTCCTGGCGCGCAGGCCCGACGTGTCTGCGGCCCAGGCCGCGGTGCTGGCGGCGCAGGCACGCGTGGGC
GTGGCGCAGGCGGCGTGGTTTCCCGCGCTGGCACTCACGGGCAGTGCGGGCTTTGCATCGCCCGAGCTGGGCGATCTGTT
CAAGTGGTCGGCGCGTTCCTGGGGCATTGGCATGCTGCTGTCGCTGCCGCTCTTCGATGGCGGCCAGCGCGCCGCCCAGG
AGGAGGGTGCGCGCGCCCGGCTGGAAGGGGCGCTCGCCGCCCACCGCGGCCAGGTGCTCACGGCCTTTCGCGAGGTGGAA
GACCAGCTCAGCGCGCTGCGCCTGCTGGCCGACCAGGCGCAGGCACAGGACCGCGCCGTGCAGTCCGCCGCGCGCGCTAC
GCAGCTGTCCGATTCGCGCTACCGCAACGGTCTGGTGAGCCAATTGGAGTTGCTGGATGCGCGCCGCAGCGAGCTGCGCA
ACCGCCGGCAGGCGCTGCAGGTGCGCACAGCGCAGTACGTGGCCACGGTGGGCTTGGTACGCGCGCTGGGCGGGGGTTGG
GATACCACCAGCTGA

Upstream 100 bases:

>100_bases
CGCTGCAGCAGCACGGTAGCCATACCGCGCCATTGATCGAAGAGACCGGCGCCGGCGGCGCGGCGCGTCCCCTGCCGGCC
GCGCCGCGCGGGCAGCACGA

Downstream 100 bases:

>100_bases
GCGCGTAGGGATTCGCGCCCACGCGACTGCGCCGCGGGGCGTTGCACTCTATAGTGACCGGTTGCGGCCGGCGCGGGTCT
TGCCCCTGCGCCTGGCGCCC

Product: NodT family RND efflux system, outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKKEFPMTMLSLAFPARLAPLAAALVLAGCMGAPLAPQPEHAGVAVPAAFSAAATEGAPAWTTAPPAEAQPRGAWWLGFQ
DPVLSDLVERAGQANTSVQEAAARLAEARALLRSADAERAPQVRANAGVSRQAGVTTTNGTQPATLATAGLGVSYELDLF
GRLARASDAARLDAQARAALLQSTRLMVQADVAQTYLQLRALQAERALVDESLEAYRGTLYLTQRRYQAGDVAELDVARV
QTEVAATESEALALERQQAQLRHALAVLVGDVATGFSLPPAAVEAALPVIPPGVPSTVLARRPDVSAAQAAVLAAQARVG
VAQAAWFPALALTGSAGFASPELGDLFKWSARSWGIGMLLSLPLFDGGQRAAQEEGARARLEGALAAHRGQVLTAFREVE
DQLSALRLLADQAQAQDRAVQSAARATQLSDSRYRNGLVSQLELLDARRSELRNRRQALQVRTAQYVATVGLVRALGGGW
DTTS

Sequences:

>Translated_484_residues
MKKEFPMTMLSLAFPARLAPLAAALVLAGCMGAPLAPQPEHAGVAVPAAFSAAATEGAPAWTTAPPAEAQPRGAWWLGFQ
DPVLSDLVERAGQANTSVQEAAARLAEARALLRSADAERAPQVRANAGVSRQAGVTTTNGTQPATLATAGLGVSYELDLF
GRLARASDAARLDAQARAALLQSTRLMVQADVAQTYLQLRALQAERALVDESLEAYRGTLYLTQRRYQAGDVAELDVARV
QTEVAATESEALALERQQAQLRHALAVLVGDVATGFSLPPAAVEAALPVIPPGVPSTVLARRPDVSAAQAAVLAAQARVG
VAQAAWFPALALTGSAGFASPELGDLFKWSARSWGIGMLLSLPLFDGGQRAAQEEGARARLEGALAAHRGQVLTAFREVE
DQLSALRLLADQAQAQDRAVQSAARATQLSDSRYRNGLVSQLELLDARRSELRNRRQALQVRTAQYVATVGLVRALGGGW
DTTS
>Mature_484_residues
MKKEFPMTMLSLAFPARLAPLAAALVLAGCMGAPLAPQPEHAGVAVPAAFSAAATEGAPAWTTAPPAEAQPRGAWWLGFQ
DPVLSDLVERAGQANTSVQEAAARLAEARALLRSADAERAPQVRANAGVSRQAGVTTTNGTQPATLATAGLGVSYELDLF
GRLARASDAARLDAQARAALLQSTRLMVQADVAQTYLQLRALQAERALVDESLEAYRGTLYLTQRRYQAGDVAELDVARV
QTEVAATESEALALERQQAQLRHALAVLVGDVATGFSLPPAAVEAALPVIPPGVPSTVLARRPDVSAAQAAVLAAQARVG
VAQAAWFPALALTGSAGFASPELGDLFKWSARSWGIGMLLSLPLFDGGQRAAQEEGARARLEGALAAHRGQVLTAFREVE
DQLSALRLLADQAQAQDRAVQSAARATQLSDSRYRNGLVSQLELLDARRSELRNRRQALQVRTAQYVATVGLVRALGGGW
DTTS

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=469, Percent_Identity=29.8507462686567, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI2367348, Length=428, Percent_Identity=27.3364485981308, Blast_Score=125, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50878; Mature: 50878

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEFPMTMLSLAFPARLAPLAAALVLAGCMGAPLAPQPEHAGVAVPAAFSAAATEGAPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHCCCCCCC
WTTAPPAEAQPRGAWWLGFQDPVLSDLVERAGQANTSVQEAAARLAEARALLRSADAERA
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCC
PQVRANAGVSRQAGVTTTNGTQPATLATAGLGVSYELDLFGRLARASDAARLDAQARAAL
CCCHHCCCCCCCCCCCCCCCCCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
LQSTRLMVQADVAQTYLQLRALQAERALVDESLEAYRGTLYLTQRRYQAGDVAELDVARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHH
QTEVAATESEALALERQQAQLRHALAVLVGDVATGFSLPPAAVEAALPVIPPGVPSTVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHH
RRPDVSAAQAAVLAAQARVGVAQAAWFPALALTGSAGFASPELGDLFKWSARSWGIGMLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCCCCHHHE
SLPLFDGGQRAAQEEGARARLEGALAAHRGQVLTAFREVEDQLSALRLLADQAQAQDRAV
ECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSAARATQLSDSRYRNGLVSQLELLDARRSELRNRRQALQVRTAQYVATVGLVRALGGGW
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DTTS
CCCC
>Mature Secondary Structure
MKKEFPMTMLSLAFPARLAPLAAALVLAGCMGAPLAPQPEHAGVAVPAAFSAAATEGAPA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCHHHHHHCCCCCCC
WTTAPPAEAQPRGAWWLGFQDPVLSDLVERAGQANTSVQEAAARLAEARALLRSADAERA
CCCCCCCCCCCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHCC
PQVRANAGVSRQAGVTTTNGTQPATLATAGLGVSYELDLFGRLARASDAARLDAQARAAL
CCCHHCCCCCCCCCCCCCCCCCCCHHEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
LQSTRLMVQADVAQTYLQLRALQAERALVDESLEAYRGTLYLTQRRYQAGDVAELDVARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHH
QTEVAATESEALALERQQAQLRHALAVLVGDVATGFSLPPAAVEAALPVIPPGVPSTVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCHHHHH
RRPDVSAAQAAVLAAQARVGVAQAAWFPALALTGSAGFASPELGDLFKWSARSWGIGMLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHCCCCCCCHHHE
SLPLFDGGQRAAQEEGARARLEGALAAHRGQVLTAFREVEDQLSALRLLADQAQAQDRAV
ECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSAARATQLSDSRYRNGLVSQLELLDARRSELRNRRQALQVRTAQYVATVGLVRALGGGW
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DTTS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]