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The map label for this gene is nuoB [H]

Identifier: 222110087

GI number: 222110087

Start: 896979

End: 897458

Strand: Direct

Name: nuoB [H]

Synonym: Dtpsy_0873

Alternate gene names: 222110087

Gene position: 896979-897458 (Clockwise)

Preceding gene: 222110086

Following gene: 222110088

Centisome position: 23.63

GC content: 61.25

Gene sequence:

>480_bases
ATGATTGAAGGTGTGATGAAGGAAGGCTTCATCACCACGAGTTATGACTCCGTGGTGAACTGGGCCAAGACCGGATCCCT
TTGGCCCATGACCTTCGGACTGGCCTGCTGCGCTGTCGAGATGATGCATGCTGCCGCCGCACGTTATGACCTGGGGCGTT
TTGGCGCGGAAGTGTTCCGGGCGAGCCCGCGACAGTCGGACCTGATGATCGTGGCCGGCACGCTGTGCAACAAGATGGCG
CCCGCTCTGCGCAAGGTGTACGACCAAATGGCCGAGCCGCGCTGGGTCATCTCCATGGGCTCTTGTGCCAACGGCGGGGG
CTACTACCACTACAGCTATTCGGTGGTGCGTGGCTGTGATCGCATCGTCCCTGTGGATGTGTACGTTCCCGGTTGTCCTC
CGACGGCCGAGGCGCTGATCTACGGGATCATTCAGCTGCAACAGAAGATCCGCCGCACCCACACCATTGCGCGCGCCTGA

Upstream 100 bases:

>100_bases
TGTGCTGGTTTTCCTGGCCATCCTCGTGGTGGGCTTTGCCTACGAATGGAAAAAAGGCGCCCTCAACTGGGAATGAGCCG
CCGCTCAAAGGAATGACGCA

Downstream 100 bases:

>100_bases
AGGGACGAAGATGACTGCTATTGCCATTCACCCTGAGAGCCTGCGGGACGTGGTTGCCTCCGCTCTGGGTGACCGCGTGC
GCCAGATCTCGGTGGCGCTG

Product: NADH dehydrogenase subunit b

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B [H]

Number of amino acids: Translated: 159; Mature: 159

Protein sequence:

>159_residues
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDLGRFGAEVFRASPRQSDLMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARA

Sequences:

>Translated_159_residues
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDLGRFGAEVFRASPRQSDLMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARA
>Mature_159_residues
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDLGRFGAEVFRASPRQSDLMIVAGTLCNKMA
PALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=253, Evalue=5e-68,
Organism=Escherichia coli, GI1788624, Length=149, Percent_Identity=53.0201342281879, Blast_Score=178, Evalue=1e-46,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=41.4634146341463, Blast_Score=93, Evalue=8e-21,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=41.4634146341463, Blast_Score=90, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17509685, Length=148, Percent_Identity=70.2702702702703, Blast_Score=236, Evalue=4e-63,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=78.0141843971631, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=78.0141843971631, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24651058, Length=141, Percent_Identity=78.7234042553192, Blast_Score=247, Evalue=3e-66,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17525; Mature: 17525

Theoretical pI: Translated: 8.57; Mature: 8.57

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDLGRFGAEVFR
CCCCHHHCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARA
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEGVMKEGFITTSYDSVVNWAKTGSLWPMTFGLACCAVEMMHAAAARYDLGRFGAEVFR
CCCCHHHCCCEEECHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASPRQSDLMIVAGTLCNKMAPALRKVYDQMAEPRWVISMGSCANGGGYYHYSYSVVRGCD
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHCCC
RIVPVDVYVPGCPPTAEALIYGIIQLQQKIRRTHTIARA
CEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA