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The map label for this gene is spoT [H]

Identifier: 222110078

GI number: 222110078

Start: 887707

End: 889971

Strand: Direct

Name: spoT [H]

Synonym: Dtpsy_0864

Alternate gene names: 222110078

Gene position: 887707-889971 (Clockwise)

Preceding gene: 222110077

Following gene: 222110081

Centisome position: 23.38

GC content: 61.37

Gene sequence:

>2265_bases
ATGAACGTCGTGTTGAACAAGGCAGCTGTTGACAATCCGCAGGTCGTCCCTGTGGCGGTTGACAGTTCTTCTGCTGCGGC
CGCCAACGCCGCGGCGGCGAGCTTTGCTGCGCTGCTGGAAAAACTCGACTACCTTGACGAAGCCAGCATCGAGCAGGTGC
GCCAGGCGTACCGTTACGCCGATGAAGCGCACTTGGGGCAGTTGCGCAGCAGTGGCGAGCCCTACATCACGCACCCGATT
GCCGTGGCGGCGCTGTGCGCGAGCTGGAAGTTGGATGTCCAGGCCCTCATGGCAGCCTTGCTGCACGACGCCATGGAAGA
TTGCGGCATCACCAAGTCCGATCTCATCGACCGGTTTGGTGCGCCTGTGGCGGAACTGGTGGATGGTCTCACCAAACTCG
ATAAGCTGCAGTTCAATACCCGTGAGGAGAACCAGGCCGAGTCGTTTCGCAAGATGCTGCTGGCCATGGCGCGCGACGTG
CGCGTCATCCTCATCAAGCTGGCGGACCGCACGCACAATATGCGCACGCTCTCCGACATGCCGCGCAGCAAGTGGGGCCG
CATTTCGTCGGAGACGCTGGAGATCTATGCGCCCATCGCGCATCGATTGGGGCTGAACCAGACCTACCGCGAACTGCAGG
ACCTCGCCTTTCGCCACCTGCATCCATGGCGCTATGCCACGCTGTCCAAAGCGGTCACGAAGTCGCGCAACCGACGCCGC
GACATCGTGCAGAAGGTGCAGACGGAAGTGGATGCCGCATTTGCGCGGCTGGGCATGCACGTGCGGCTGGCGGGACGTGA
GAAGACACTCTTTGCGATCTACCAGAAGATGATCACCAAGCACCTGAGCTTTGCGCAGGTGACGGATCTCTATGGCTTCC
GTGTCATCGTGCATTCGGTGACGGACTGCTATACCGCCTTGGGTGTGTTGCACCAGATGTACAAACCGGTGCCCGGCAAG
TTCAAGGACCATATCGCCATTGGCAAGCTCAATGGCTATCAGTCGCTGCATACGACGCTGGTGGGACCTGCTGGCCTGAA
CATCGAGTTTCAGGTTCGCACCGAGGAAATGCACATCGTGGCGGAAGCGGGTGTGGCGGCGCACTGGCTCTACAAGGCGA
AAGATGCGGATGGCGCGGTGGCAGAACGCCTGGGCACCAAATGGCTGCAGTCCTTGCTCGACATCCAGAATGAAACGCGC
GACGCGGCCGAATTCTGGGACCACGTCAAGGTGGACCTGTTCCCAGATGCCGTCTATGTCTTCACGCCCAAGAGCCAGAT
CATGGCCTTGCCCCGCGGAGCCACTGCGGTGGACTTTGCCTATGCGATCCACAGCGACGTGGGCGACCGCACCACGGCGG
CGCGCATCAACAACGAACAGGTGCCCCTGCGCACCGAACTCAAGAACGGGGATGTGGTGGAAATCATTACCACACAGGGC
GCCCGGCCCAACCCGGCTTGGCTGGGGTTCGTGCGCACAGGGCGTGCGCGTTCCAAGATTCGCCACTATCTCAAGACGCT
GGCGCAGAACGAGTCGGCGGGCCTGGGCGAGAAACTGCTGACCCAGGCGCTGCGCGCCGAGGGCATCGAACAACTGCCTG
ACGTGCAGACCCAAGCGCCCATTTGGGAAAAACTGCTGCGCTTTACGGGCAATCGCAGCCGCGCGGAACTCATGACCGAT
GTGGGACTGGGCAAGCGCATTGCCGGCATCGTGGCCAAGCGCCTGGTCGTGTTGCTATCGGAAGATGGCCAGCGGCCGGA
TGCATTGCTGCTGACCCGCGAGCGCTACAGCTCCCATGAAACAGTCTCGCAAGGGAGCATCACCCTCGACGGCAGCGAGA
ATGCATCTGTTCAATATGCAGCGTGTTGCCGCCCGGTGCCGGGTGACCCCATCGTGGGATACCTGGGCCGCGGCGAGGGC
CTGGTGGTCCATCACGCGCAATGCGGGGTAGCCAAGAAACTGCAGCACAAAGATGGCGAGCGCTTCATTGCCGTCGACTG
GTCCGATGAGCCTACCCGCCAATTCGAGACCGGTATCACCGTGACCGTGACCAATGGCAAGGGGGTACTGGCTCGCATCG
CATCCGAATTTGCCAACTCAGAGGCAGATATCGTCCGTGTGGACATGGACGATGCGATGGACACCACTGATCTGCGTTTT
GTGGCAGCGGTGCGTGATCAACACCATGTAGAGGCCGTGTTGCGCAATCTGCGCCGCACGCATTCCGTATTGCGAGCGTT
CCGCGTGCTTCCCCAGCCCGCTTGA

Upstream 100 bases:

>100_bases
CGATCGTCAGCCTCCGGTGATCCCGGTAAAAGAGCACCGCGTGGCGGTGCTTTTTTTTGCCTGCGCTTGCCGCTCGGGCG
GGGCGCGCTACATTTAAGCC

Downstream 100 bases:

>100_bases
GGGCCTGGGTTCGTCACCGAGGCGCCGCGACAGGGTATTCGACACGCAGGATTTCCAGGGTACGTAGGCCGGCGGGAGTC
TGCAGGGTCACTTCGTCTCC

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MNVVLNKAAVDNPQVVPVAVDSSSAAAANAAAASFAALLEKLDYLDEASIEQVRQAYRYADEAHLGQLRSSGEPYITHPI
AVAALCASWKLDVQALMAALLHDAMEDCGITKSDLIDRFGAPVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDV
RVILIKLADRTHNMRTLSDMPRSKWGRISSETLEIYAPIAHRLGLNQTYRELQDLAFRHLHPWRYATLSKAVTKSRNRRR
DIVQKVQTEVDAAFARLGMHVRLAGREKTLFAIYQKMITKHLSFAQVTDLYGFRVIVHSVTDCYTALGVLHQMYKPVPGK
FKDHIAIGKLNGYQSLHTTLVGPAGLNIEFQVRTEEMHIVAEAGVAAHWLYKAKDADGAVAERLGTKWLQSLLDIQNETR
DAAEFWDHVKVDLFPDAVYVFTPKSQIMALPRGATAVDFAYAIHSDVGDRTTAARINNEQVPLRTELKNGDVVEIITTQG
ARPNPAWLGFVRTGRARSKIRHYLKTLAQNESAGLGEKLLTQALRAEGIEQLPDVQTQAPIWEKLLRFTGNRSRAELMTD
VGLGKRIAGIVAKRLVVLLSEDGQRPDALLLTRERYSSHETVSQGSITLDGSENASVQYAACCRPVPGDPIVGYLGRGEG
LVVHHAQCGVAKKLQHKDGERFIAVDWSDEPTRQFETGITVTVTNGKGVLARIASEFANSEADIVRVDMDDAMDTTDLRF
VAAVRDQHHVEAVLRNLRRTHSVLRAFRVLPQPA

Sequences:

>Translated_754_residues
MNVVLNKAAVDNPQVVPVAVDSSSAAAANAAAASFAALLEKLDYLDEASIEQVRQAYRYADEAHLGQLRSSGEPYITHPI
AVAALCASWKLDVQALMAALLHDAMEDCGITKSDLIDRFGAPVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDV
RVILIKLADRTHNMRTLSDMPRSKWGRISSETLEIYAPIAHRLGLNQTYRELQDLAFRHLHPWRYATLSKAVTKSRNRRR
DIVQKVQTEVDAAFARLGMHVRLAGREKTLFAIYQKMITKHLSFAQVTDLYGFRVIVHSVTDCYTALGVLHQMYKPVPGK
FKDHIAIGKLNGYQSLHTTLVGPAGLNIEFQVRTEEMHIVAEAGVAAHWLYKAKDADGAVAERLGTKWLQSLLDIQNETR
DAAEFWDHVKVDLFPDAVYVFTPKSQIMALPRGATAVDFAYAIHSDVGDRTTAARINNEQVPLRTELKNGDVVEIITTQG
ARPNPAWLGFVRTGRARSKIRHYLKTLAQNESAGLGEKLLTQALRAEGIEQLPDVQTQAPIWEKLLRFTGNRSRAELMTD
VGLGKRIAGIVAKRLVVLLSEDGQRPDALLLTRERYSSHETVSQGSITLDGSENASVQYAACCRPVPGDPIVGYLGRGEG
LVVHHAQCGVAKKLQHKDGERFIAVDWSDEPTRQFETGITVTVTNGKGVLARIASEFANSEADIVRVDMDDAMDTTDLRF
VAAVRDQHHVEAVLRNLRRTHSVLRAFRVLPQPA
>Mature_754_residues
MNVVLNKAAVDNPQVVPVAVDSSSAAAANAAAASFAALLEKLDYLDEASIEQVRQAYRYADEAHLGQLRSSGEPYITHPI
AVAALCASWKLDVQALMAALLHDAMEDCGITKSDLIDRFGAPVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDV
RVILIKLADRTHNMRTLSDMPRSKWGRISSETLEIYAPIAHRLGLNQTYRELQDLAFRHLHPWRYATLSKAVTKSRNRRR
DIVQKVQTEVDAAFARLGMHVRLAGREKTLFAIYQKMITKHLSFAQVTDLYGFRVIVHSVTDCYTALGVLHQMYKPVPGK
FKDHIAIGKLNGYQSLHTTLVGPAGLNIEFQVRTEEMHIVAEAGVAAHWLYKAKDADGAVAERLGTKWLQSLLDIQNETR
DAAEFWDHVKVDLFPDAVYVFTPKSQIMALPRGATAVDFAYAIHSDVGDRTTAARINNEQVPLRTELKNGDVVEIITTQG
ARPNPAWLGFVRTGRARSKIRHYLKTLAQNESAGLGEKLLTQALRAEGIEQLPDVQTQAPIWEKLLRFTGNRSRAELMTD
VGLGKRIAGIVAKRLVVLLSEDGQRPDALLLTRERYSSHETVSQGSITLDGSENASVQYAACCRPVPGDPIVGYLGRGEG
LVVHHAQCGVAKKLQHKDGERFIAVDWSDEPTRQFETGITVTVTNGKGVLARIASEFANSEADIVRVDMDDAMDTTDLRF
VAAVRDQHHVEAVLRNLRRTHSVLRAFRVLPQPA

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=706, Percent_Identity=44.1926345609065, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1789147, Length=667, Percent_Identity=32.383808095952, Blast_Score=358, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 83671; Mature: 83671

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVVLNKAAVDNPQVVPVAVDSSSAAAANAAAASFAALLEKLDYLDEASIEQVRQAYRYA
CCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
DEAHLGQLRSSGEPYITHPIAVAALCASWKLDVQALMAALLHDAMEDCGITKSDLIDRFG
CHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHC
APVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRTLSDM
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHHC
PRSKWGRISSETLEIYAPIAHRLGLNQTYRELQDLAFRHLHPWRYATLSKAVTKSRNRRR
CHHHCCCCCCCHHHEEHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DIVQKVQTEVDAAFARLGMHVRLAGREKTLFAIYQKMITKHLSFAQVTDLYGFRVIVHSV
HHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDCYTALGVLHQMYKPVPGKFKDHIAIGKLNGYQSLHTTLVGPAGLNIEFQVRTEEMHIV
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHEECCCCCCEEEEEECCCEEEE
AEAGVAAHWLYKAKDADGAVAERLGTKWLQSLLDIQNETRDAAEFWDHVKVDLFPDAVYV
ECCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCEEEE
FTPKSQIMALPRGATAVDFAYAIHSDVGDRTTAARINNEQVPLRTELKNGDVVEIITTQG
ECCHHHHEECCCCCCHHHHHHHHHCCCCCCHHHEECCCCCCCEEEECCCCCEEEEEECCC
ARPNPAWLGFVRTGRARSKIRHYLKTLAQNESAGLGEKLLTQALRAEGIEQLPDVQTQAP
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCH
IWEKLLRFTGNRSRAELMTDVGLGKRIAGIVAKRLVVLLSEDGQRPDALLLTRERYSSHE
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCCC
TVSQGSITLDGSENASVQYAACCRPVPGDPIVGYLGRGEGLVVHHAQCGVAKKLQHKDGE
CCCCCCEEECCCCCCCEEEHHHCCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHCCCCC
RFIAVDWSDEPTRQFETGITVTVTNGKGVLARIASEFANSEADIVRVDMDDAMDTTDLRF
EEEEEECCCCCCHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHH
VAAVRDQHHVEAVLRNLRRTHSVLRAFRVLPQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVVLNKAAVDNPQVVPVAVDSSSAAAANAAAASFAALLEKLDYLDEASIEQVRQAYRYA
CCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
DEAHLGQLRSSGEPYITHPIAVAALCASWKLDVQALMAALLHDAMEDCGITKSDLIDRFG
CHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHC
APVAELVDGLTKLDKLQFNTREENQAESFRKMLLAMARDVRVILIKLADRTHNMRTLSDM
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCHHEEEEEECCCCCCCHHHHHC
PRSKWGRISSETLEIYAPIAHRLGLNQTYRELQDLAFRHLHPWRYATLSKAVTKSRNRRR
CHHHCCCCCCCHHHEEHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DIVQKVQTEVDAAFARLGMHVRLAGREKTLFAIYQKMITKHLSFAQVTDLYGFRVIVHSV
HHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TDCYTALGVLHQMYKPVPGKFKDHIAIGKLNGYQSLHTTLVGPAGLNIEFQVRTEEMHIV
HHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHEECCCCCCEEEEEECCCEEEE
AEAGVAAHWLYKAKDADGAVAERLGTKWLQSLLDIQNETRDAAEFWDHVKVDLFPDAVYV
ECCCHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCEEEEECCEEEE
FTPKSQIMALPRGATAVDFAYAIHSDVGDRTTAARINNEQVPLRTELKNGDVVEIITTQG
ECCHHHHEECCCCCCHHHHHHHHHCCCCCCHHHEECCCCCCCEEEECCCCCEEEEEECCC
ARPNPAWLGFVRTGRARSKIRHYLKTLAQNESAGLGEKLLTQALRAEGIEQLPDVQTQAP
CCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCCH
IWEKLLRFTGNRSRAELMTDVGLGKRIAGIVAKRLVVLLSEDGQRPDALLLTRERYSSHE
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHCCCCC
TVSQGSITLDGSENASVQYAACCRPVPGDPIVGYLGRGEGLVVHHAQCGVAKKLQHKDGE
CCCCCCEEECCCCCCCEEEHHHCCCCCCCCCEEEECCCCCEEEEECCCCHHHHHHCCCCC
RFIAVDWSDEPTRQFETGITVTVTNGKGVLARIASEFANSEADIVRVDMDDAMDTTDLRF
EEEEEECCCCCCHHHHCCEEEEEECCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHH
VAAVRDQHHVEAVLRNLRRTHSVLRAFRVLPQPA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]