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The map label for this gene is pinE [H]

Identifier: 222110060

GI number: 222110060

Start: 874365

End: 874949

Strand: Direct

Name: pinE [H]

Synonym: Dtpsy_0846

Alternate gene names: 222110060

Gene position: 874365-874949 (Clockwise)

Preceding gene: 222110058

Following gene: 222110061

Centisome position: 23.03

GC content: 64.27

Gene sequence:

>585_bases
GTGACCGTATGGGGATATGCGCGCGTGTCGACGCTGGAACAGAACACTGCAGCTCAGCTCGCGGCGTTCCGGCGTGCAGG
CATTGAGCAGGTCGTTGAAGAAAAGCGCTCGGGCGTGAAGGACCGCCCCGAGCTCGAGAAGCTGCTACTCCAGCTGCAGG
CCGGTGACGTCCTGGTCGTCTACAAGCTGGACCGTTTGGCGCGGTCCGTGTCGCACTTCGTGAAGGTGTTCGACCAGCTC
AAGGCGAAGGGCGTGGGCTTTCGTTCGTTGACCGAAGCCATAGAGACCGACACGCCGCAAGGACGCATGTTCCTGCACCT
GTTGACGGCGTTCGCCGAGTTCGAGAGGGAGTTGATCCGGGAGCGGTGCCTGGCTGGGCAACGTGCCGCGCGTGCGGCGG
GGAAGACGTGGGGGAGAAAGCGGGTTCTGTCGGACGACGACGCGGCTCAAGCGGCAAATGCGTGGCGCAGTGGTTGGTAC
ACCCAGGCCGTATTGGCGGACATGCTGGGCGTCACCGTTGCTTGCCTGCGAGACTGCATCCACCGGCATGAGCGTCGGGG
CCGGTGGGCGGGGTTACCAACGTAA

Upstream 100 bases:

>100_bases
CCTGCAGCGGAAGGGTCTGGACTCCAGAGGAGATCGGAGATCTCCGGCAGGAGTCTGGAGCCTGTAGCGGGATAGCATTG
CCCAAAAGGGAGGGCAGGGC

Downstream 100 bases:

>100_bases
AATGATTCAATGCTTGATGACCGCTCCAAGTTGTTACTCAAGGCCTTGGTGGAACGCTACATAGCCGAGGGGCAGCCGGT
CGGCTCCAGAACCTTGTCGC

Product: resolvase domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 194; Mature: 193

Protein sequence:

>194_residues
MTVWGYARVSTLEQNTAAQLAAFRRAGIEQVVEEKRSGVKDRPELEKLLLQLQAGDVLVVYKLDRLARSVSHFVKVFDQL
KAKGVGFRSLTEAIETDTPQGRMFLHLLTAFAEFERELIRERCLAGQRAARAAGKTWGRKRVLSDDDAAQAANAWRSGWY
TQAVLADMLGVTVACLRDCIHRHERRGRWAGLPT

Sequences:

>Translated_194_residues
MTVWGYARVSTLEQNTAAQLAAFRRAGIEQVVEEKRSGVKDRPELEKLLLQLQAGDVLVVYKLDRLARSVSHFVKVFDQL
KAKGVGFRSLTEAIETDTPQGRMFLHLLTAFAEFERELIRERCLAGQRAARAAGKTWGRKRVLSDDDAAQAANAWRSGWY
TQAVLADMLGVTVACLRDCIHRHERRGRWAGLPT
>Mature_193_residues
TVWGYARVSTLEQNTAAQLAAFRRAGIEQVVEEKRSGVKDRPELEKLLLQLQAGDVLVVYKLDRLARSVSHFVKVFDQLK
AKGVGFRSLTEAIETDTPQGRMFLHLLTAFAEFERELIRERCLAGQRAARAAGKTWGRKRVLSDDDAAQAANAWRSGWYT
QAVLADMLGVTVACLRDCIHRHERRGRWAGLPT

Specific function: This protein catalyzes the inversion of an 1800-bp E.coli DNA fragment, the P region, which can exist in either orientation. The function of the inversion is not yet clear [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=161, Percent_Identity=50.3105590062112, Blast_Score=145, Evalue=1e-36,
Organism=Escherichia coli, GI1787827, Length=170, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=5e-23,
Organism=Escherichia coli, GI1787638, Length=170, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 21837; Mature: 21706

Theoretical pI: Translated: 10.29; Mature: 10.29

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVWGYARVSTLEQNTAAQLAAFRRAGIEQVVEEKRSGVKDRPELEKLLLQLQAGDVLVV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEE
YKLDRLARSVSHFVKVFDQLKAKGVGFRSLTEAIETDTPQGRMFLHLLTAFAEFERELIR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERCLAGQRAARAAGKTWGRKRVLSDDDAAQAANAWRSGWYTQAVLADMLGVTVACLRDCI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
HRHERRGRWAGLPT
HHHHHCCCCCCCCC
>Mature Secondary Structure 
TVWGYARVSTLEQNTAAQLAAFRRAGIEQVVEEKRSGVKDRPELEKLLLQLQAGDVLVV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEE
YKLDRLARSVSHFVKVFDQLKAKGVGFRSLTEAIETDTPQGRMFLHLLTAFAEFERELIR
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ERCLAGQRAARAAGKTWGRKRVLSDDDAAQAANAWRSGWYTQAVLADMLGVTVACLRDCI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
HRHERRGRWAGLPT
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3894006; 6310572; 8905232; 9278503 [H]