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The map label for this gene is gltD [H]

Identifier: 222109925

GI number: 222109925

Start: 742663

End: 744141

Strand: Direct

Name: gltD [H]

Synonym: Dtpsy_0710

Alternate gene names: 222109925

Gene position: 742663-744141 (Clockwise)

Preceding gene: 222109924

Following gene: 222109926

Centisome position: 19.56

GC content: 66.4

Gene sequence:

>1479_bases
ATGGGCAAGACCACAGGTTTCATGGAATACGAGCGCATCGAGGAGGGCTACAAGCCCGTTGGCGAGCGCCTCAAACACTA
CAAGGAATTCGTCATCGGCCTTGATGACGCCCAGGCCAAGCTACAGGGCGCCCGCTGCATGGATTGCGGCACGCCGTTCT
GCAACAACGGCTGCCCGGTGAACAACATCATCCCGGACTTCAACGACCTCGTTTACCACCAGGACTGGAAGAGCGCGATC
GCGGTGCTGCACAGCACCAACAACTTCCCCGAGTTCACCGGCCGCATCTGCCCCGCACCCTGCGAGGCCGCCTGCACGCT
GAACATCAACGACGACGCGGTGGGCATCAAGAGCATCGAGCACGCCATCATCGACCGCGCCTGGGAGCACGGCTGGGTGC
AGCCGCAGCCGGCTGCCCGCAAGACGGGCAAGAAAGTGGCCGTGGTGGGCGCCGGCCCGGCGGGCCTGGCGGCCGCGCAG
CAACTGGCGCGCGCCGGGCATGACGTCACGCTGTTCGAGAAGAACGACCGCGTGGGCGGCCTGCTGCGCTACGGCATCCC
CGACTTCAAGCTGGACAAGGCGCACATCGACCGCCGCGTGGAGCAACTGGTGGCCGAGGGCGTGACGATCCGTACCGGCG
TCTTCATCGGCGCGGCCAAGGACGGGTTGGGCAAAGGCAGCAAGGTCACCAACTGGTCCAAGGAAACCATCACGCCCGAG
CAGCTGCGCAAGGACTTCGACGCAGTGCTGCTGACGGGTGGCGCCGAGCAGTCGCGCGACCTGCCCGTGCCGGGGCGCGA
ACTGGACGGCATTCACTTCGCGATGGAGTTCCTGCCGCAGCAGAACAAGGTCAACGCGGGCGACAAGCTCAAGGGCCAGA
TCCGCGCGGACGGCAAGCACGTCATCGTCATCGGCGGCGGCGACACGGGCAGCGACTGTGTGGGCACCAGCAATCGCCAT
GGCGCGGCCAGCGTCACACAGTTCGAGCTGATGCCCATGCCGCCAAGCCAAGAGAACAAGCCCCTGGTGTGGCCCTACTG
GCCGTACAAGCTGCGCACCAGCTCCAGCCACGAGGAGGGCTGCGAGCGCGCGTTCGCCATCGCCACCAAGGAGTTCATCG
GCGACGGCAAGAAGGTCACGGGCCTCAAGACCGTGCAGGTGGAGTTCAAGGATGGCAAGTTCATCGAGGTCCCCGGCACC
GAGAAGGAATACCAGGCCGACCTGGTGCTGCTGGCCATGGGTTTCGTAAGCCCGGTGGCTGCCGTGCTGGACGCCTTCGG
AGTGGACAAGGATGCGCGGGGCAATGCCCAGGCCACGACGGACTTCACCGGCGGCTATGCGACCAGCGCGCCCAAGGTGT
TCGCCGCCGGCGACATGCGCCGCGGCCAGTCGCTGGTGGTCTGGGCCATCCGCGAAGGCCGCCAGGCCGCGCGTGCGGTG
GATGAGTTCCTGATGGGGGAAAGCAGTCTGCCGCGCTGA

Upstream 100 bases:

>100_bases
AGCAGCTCCTAAAACAGAAGCGGTAGCCGCCAAGTAACCCGCGCGGGCGGGGCCTGGGGCCCCGCTGCGGCAGCATGCAC
AAAGACAGGACACCACGATC

Downstream 100 bases:

>100_bases
GGCGGGAATCCCTCGCCATTGGGCAATTACAGAAGGCGGTCGCAGCCGCAGTCTACTTACCCGTCTACCCGCACAACGGC
GCCCCAGGGCGCCGTTGTGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 492; Mature: 491

Protein sequence:

>492_residues
MGKTTGFMEYERIEEGYKPVGERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPVNNIIPDFNDLVYHQDWKSAI
AVLHSTNNFPEFTGRICPAPCEAACTLNINDDAVGIKSIEHAIIDRAWEHGWVQPQPAARKTGKKVAVVGAGPAGLAAAQ
QLARAGHDVTLFEKNDRVGGLLRYGIPDFKLDKAHIDRRVEQLVAEGVTIRTGVFIGAAKDGLGKGSKVTNWSKETITPE
QLRKDFDAVLLTGGAEQSRDLPVPGRELDGIHFAMEFLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRH
GAASVTQFELMPMPPSQENKPLVWPYWPYKLRTSSSHEEGCERAFAIATKEFIGDGKKVTGLKTVQVEFKDGKFIEVPGT
EKEYQADLVLLAMGFVSPVAAVLDAFGVDKDARGNAQATTDFTGGYATSAPKVFAAGDMRRGQSLVVWAIREGRQAARAV
DEFLMGESSLPR

Sequences:

>Translated_492_residues
MGKTTGFMEYERIEEGYKPVGERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPVNNIIPDFNDLVYHQDWKSAI
AVLHSTNNFPEFTGRICPAPCEAACTLNINDDAVGIKSIEHAIIDRAWEHGWVQPQPAARKTGKKVAVVGAGPAGLAAAQ
QLARAGHDVTLFEKNDRVGGLLRYGIPDFKLDKAHIDRRVEQLVAEGVTIRTGVFIGAAKDGLGKGSKVTNWSKETITPE
QLRKDFDAVLLTGGAEQSRDLPVPGRELDGIHFAMEFLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRH
GAASVTQFELMPMPPSQENKPLVWPYWPYKLRTSSSHEEGCERAFAIATKEFIGDGKKVTGLKTVQVEFKDGKFIEVPGT
EKEYQADLVLLAMGFVSPVAAVLDAFGVDKDARGNAQATTDFTGGYATSAPKVFAAGDMRRGQSLVVWAIREGRQAARAV
DEFLMGESSLPR
>Mature_491_residues
GKTTGFMEYERIEEGYKPVGERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPVNNIIPDFNDLVYHQDWKSAIA
VLHSTNNFPEFTGRICPAPCEAACTLNINDDAVGIKSIEHAIIDRAWEHGWVQPQPAARKTGKKVAVVGAGPAGLAAAQQ
LARAGHDVTLFEKNDRVGGLLRYGIPDFKLDKAHIDRRVEQLVAEGVTIRTGVFIGAAKDGLGKGSKVTNWSKETITPEQ
LRKDFDAVLLTGGAEQSRDLPVPGRELDGIHFAMEFLPQQNKVNAGDKLKGQIRADGKHVIVIGGGDTGSDCVGTSNRHG
AASVTQFELMPMPPSQENKPLVWPYWPYKLRTSSSHEEGCERAFAIATKEFIGDGKKVTGLKTVQVEFKDGKFIEVPGTE
KEYQADLVLLAMGFVSPVAAVLDAFGVDKDARGNAQATTDFTGGYATSAPKVFAAGDMRRGQSLVVWAIREGRQAARAVD
EFLMGESSLPR

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=471, Percent_Identity=24.6284501061571, Blast_Score=103, Evalue=3e-22,
Organism=Escherichia coli, GI1789606, Length=491, Percent_Identity=35.4378818737271, Blast_Score=256, Evalue=3e-69,
Organism=Escherichia coli, GI1788811, Length=474, Percent_Identity=33.5443037974684, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87082180, Length=476, Percent_Identity=34.0336134453782, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1788468, Length=453, Percent_Identity=30.0220750551876, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789243, Length=440, Percent_Identity=28.1818181818182, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17570289, Length=511, Percent_Identity=44.0313111545988, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71984108, Length=310, Percent_Identity=26.1290322580645, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320030, Length=505, Percent_Identity=47.1287128712871, Blast_Score=449, Evalue=1e-127,
Organism=Drosophila melanogaster, GI28574881, Length=507, Percent_Identity=50.2958579881657, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665539, Length=507, Percent_Identity=50.2958579881657, Blast_Score=481, Evalue=1e-136,
Organism=Drosophila melanogaster, GI24665547, Length=507, Percent_Identity=50.2958579881657, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24665543, Length=507, Percent_Identity=50.2958579881657, Blast_Score=479, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24640763, Length=306, Percent_Identity=26.1437908496732, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18858217, Length=306, Percent_Identity=26.1437908496732, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53387; Mature: 53256

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTTGFMEYERIEEGYKPVGERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCCCCCCCC
NNIIPDFNDLVYHQDWKSAIAVLHSTNNFPEFTGRICPAPCEAACTLNINDDAVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCHHHHH
HAIIDRAWEHGWVQPQPAARKTGKKVAVVGAGPAGLAAAQQLARAGHDVTLFEKNDRVGG
HHHHHHHHHCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLDKAHIDRRVEQLVAEGVTIRTGVFIGAAKDGLGKGSKVTNWSKETITPE
EEECCCCCCEECHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHH
QLRKDFDAVLLTGGAEQSRDLPVPGRELDGIHFAMEFLPQQNKVNAGDKLKGQIRADGKH
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCE
VIVIGGGDTGSDCVGTSNRHGAASVTQFELMPMPPSQENKPLVWPYWPYKLRTSSSHEEG
EEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCCEEEECCCCHHHH
CERAFAIATKEFIGDGKKVTGLKTVQVEFKDGKFIEVPGTEKEYQADLVLLAMGFVSPVA
HHHHHHHHHHHHHCCCCEECCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
AVLDAFGVDKDARGNAQATTDFTGGYATSAPKVFAAGDMRRGQSLVVWAIREGRQAARAV
HHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHH
DEFLMGESSLPR
HHHHHCCCCCCC
>Mature Secondary Structure 
GKTTGFMEYERIEEGYKPVGERLKHYKEFVIGLDDAQAKLQGARCMDCGTPFCNNGCPV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCEEECCCCCCCCCCCCC
NNIIPDFNDLVYHQDWKSAIAVLHSTNNFPEFTGRICPAPCEAACTLNINDDAVGIKSIE
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCCEEEEECCCCCCCHHHHH
HAIIDRAWEHGWVQPQPAARKTGKKVAVVGAGPAGLAAAQQLARAGHDVTLFEKNDRVGG
HHHHHHHHHCCCCCCCCHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCC
LLRYGIPDFKLDKAHIDRRVEQLVAEGVTIRTGVFIGAAKDGLGKGSKVTNWSKETITPE
EEECCCCCCEECHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHH
QLRKDFDAVLLTGGAEQSRDLPVPGRELDGIHFAMEFLPQQNKVNAGDKLKGQIRADGKH
HHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCEEEECCCE
VIVIGGGDTGSDCVGTSNRHGAASVTQFELMPMPPSQENKPLVWPYWPYKLRTSSSHEEG
EEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCEEECCCCEEEECCCCHHHH
CERAFAIATKEFIGDGKKVTGLKTVQVEFKDGKFIEVPGTEKEYQADLVLLAMGFVSPVA
HHHHHHHHHHHHHCCCCEECCEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
AVLDAFGVDKDARGNAQATTDFTGGYATSAPKVFAAGDMRRGQSLVVWAIREGRQAARAV
HHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCEEECCCCCCCCEEEEEEEHHHHHHHHHH
DEFLMGESSLPR
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]