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The map label for this gene is narK [H]

Identifier: 222109646

GI number: 222109646

Start: 435650

End: 437095

Strand: Direct

Name: narK [H]

Synonym: Dtpsy_0426

Alternate gene names: 222109646

Gene position: 435650-437095 (Clockwise)

Preceding gene: 222109645

Following gene: 222109647

Centisome position: 11.47

GC content: 63.14

Gene sequence:

>1446_bases
ATGTCCACCAACACCGTTTCGCAAGGCAGCGGCCGCAAAGGCCGGGTGCTCACGCTCTGGACCCCCGAGGACAAGTCCTT
CTGGGCGCGAGAGGGCGAGGCTGTCGCCAAGATCAATCTCTGGATCTCGGTGCCCGCACTGTTCCTGGCCTTCGCCATCT
GGCAGGTCTGGAGTGTGGTCGCCGTGAACTTGCCGGCACTGGGTTTCAAGTATTCGACCAACCAGCTGTTCTGGCTGGCT
GCCGCCCCGGCGTTGTCCGGCGCCACGCTGCGTATCTTCTATTCCTTCATGGTGCCCATCTTCGGCGGCCGCCGCTGGAC
TGCGCTGTCCACCGCGTCGCTGCTGGTCCCCGCCATCGGCATCGGCTATGCGGTGCAGGACAACACCACCAGCTACCCCA
CCATGCTTTTGTTGGCGCTGCTGTGCGGCCTGGGCGGCGGTAACTTCAGCTCCAGCATGGCCAATATCAGCTTCTTCTTT
CCGAAGGAGCGCAAGGGTTCCGCGCTGGGTGTGAATGCCGGCCTGGGCAACCTGGGGGTGTCGGTGGTGCAGTTCCTGAG
CCCGCTGGTCATTACGGCAGGCATCTTCGGTATCTTTGGCGGGGAGCCCCAGACCATCGTGAAGAATGGCCAGGAGCAGA
TGGTGTGGGCGCAGAACGCCGCCTTCATCTGGGTGCCCTGGATCGCACTGGCCGCGGTGGCGGCGTGGTTCGGCATGAAC
GACATTGCCGATGCGCGTGCCAGCTTCGCCGCGCAGGCGGCCATCTTCAAGCGCAAGCACAACTGGATCATGTGCGTGCT
GTACCTGGGCACCTTCGGTTCGTTCATCGGTTTTGCCGCAGGCTTTCCGCTGCTGATCAAGGCGCTCTTCCCTGAAGTCA
ACCCCCTGGCCTACGCCTGGCTGGGTCCGCTAGTGGGCGCCGTGGTGCGTCCGTTCGGCGGCTGGCTGGCCGACAAGGTG
GGCGGTGCCCGGGTCACGGTCTGGAACTTCCTCGTCATGGCGCTGGCCGTGGTGGGTGTTCTGTTTTTCCTACCCAAGGG
CTCGGGCGGTTTTGCGCTGCCCTTTGGCCCGGCTGAAGGCAGCTTCACGGGCTTCTTCCTGATGTTCCTGGTACTCTTCT
TCACCACCGGCATCGGCAACGGCTCCACCTTCCGCATGATCCCCGTGATATTTCTGGCCCAGAAGCTGCGCGAGTTGCGC
ACCGATGACGAAGCATCGCGCGCCCAAGCCATCAAGGATGGCAACACCGAAGGCGCGGCCGCGGTGGGCTTCGCCGGAGC
GCTGGGCGCCTACGGGGGCTTTTTCATTCCCAAGAGCTATGGCAGCTCCATCGCCGCGACGGGCGGTCCCGAATTCGCGC
TGTGGATGTTTGCCGTTTTCTATGTTCTCTGCATCATCATCACTTGGTGGTACTACTCTCGCAAGCATGCAGAGATGCCC
TGCTGA

Upstream 100 bases:

>100_bases
TCGTTGATCTGGATGTATTTCACCGAGGTGCGCCGCACCGACGTGATGAAGTCTCACAACGCCGCCCCGGCTGCCCACTG
ATCCCCACCGGAGATCCCAC

Downstream 100 bases:

>100_bases
GCCACGCCACTACAACGATTTCCAAGGAGTCCCACGATGAGCCATTTCCTAGACCGTCTCTCGTACTTTGCGCAGCCGCG
CGAAACCTTCTCGCAGGGCC

Product: nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSTNTVSQGSGRKGRVLTLWTPEDKSFWAREGEAVAKINLWISVPALFLAFAIWQVWSVVAVNLPALGFKYSTNQLFWLA
AAPALSGATLRIFYSFMVPIFGGRRWTALSTASLLVPAIGIGYAVQDNTTSYPTMLLLALLCGLGGGNFSSSMANISFFF
PKERKGSALGVNAGLGNLGVSVVQFLSPLVITAGIFGIFGGEPQTIVKNGQEQMVWAQNAAFIWVPWIALAAVAAWFGMN
DIADARASFAAQAAIFKRKHNWIMCVLYLGTFGSFIGFAAGFPLLIKALFPEVNPLAYAWLGPLVGAVVRPFGGWLADKV
GGARVTVWNFLVMALAVVGVLFFLPKGSGGFALPFGPAEGSFTGFFLMFLVLFFTTGIGNGSTFRMIPVIFLAQKLRELR
TDDEASRAQAIKDGNTEGAAAVGFAGALGAYGGFFIPKSYGSSIAATGGPEFALWMFAVFYVLCIIITWWYYSRKHAEMP
C

Sequences:

>Translated_481_residues
MSTNTVSQGSGRKGRVLTLWTPEDKSFWAREGEAVAKINLWISVPALFLAFAIWQVWSVVAVNLPALGFKYSTNQLFWLA
AAPALSGATLRIFYSFMVPIFGGRRWTALSTASLLVPAIGIGYAVQDNTTSYPTMLLLALLCGLGGGNFSSSMANISFFF
PKERKGSALGVNAGLGNLGVSVVQFLSPLVITAGIFGIFGGEPQTIVKNGQEQMVWAQNAAFIWVPWIALAAVAAWFGMN
DIADARASFAAQAAIFKRKHNWIMCVLYLGTFGSFIGFAAGFPLLIKALFPEVNPLAYAWLGPLVGAVVRPFGGWLADKV
GGARVTVWNFLVMALAVVGVLFFLPKGSGGFALPFGPAEGSFTGFFLMFLVLFFTTGIGNGSTFRMIPVIFLAQKLRELR
TDDEASRAQAIKDGNTEGAAAVGFAGALGAYGGFFIPKSYGSSIAATGGPEFALWMFAVFYVLCIIITWWYYSRKHAEMP
C
>Mature_480_residues
STNTVSQGSGRKGRVLTLWTPEDKSFWAREGEAVAKINLWISVPALFLAFAIWQVWSVVAVNLPALGFKYSTNQLFWLAA
APALSGATLRIFYSFMVPIFGGRRWTALSTASLLVPAIGIGYAVQDNTTSYPTMLLLALLCGLGGGNFSSSMANISFFFP
KERKGSALGVNAGLGNLGVSVVQFLSPLVITAGIFGIFGGEPQTIVKNGQEQMVWAQNAAFIWVPWIALAAVAAWFGMND
IADARASFAAQAAIFKRKHNWIMCVLYLGTFGSFIGFAAGFPLLIKALFPEVNPLAYAWLGPLVGAVVRPFGGWLADKVG
GARVTVWNFLVMALAVVGVLFFLPKGSGGFALPFGPAEGSFTGFFLMFLVLFFTTGIGNGSTFRMIPVIFLAQKLRELRT
DDEASRAQAIKDGNTEGAAAVGFAGALGAYGGFFIPKSYGSSIAATGGPEFALWMFAVFYVLCIIITWWYYSRKHAEMPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=466, Percent_Identity=51.931330472103, Blast_Score=449, Evalue=1e-127,
Organism=Escherichia coli, GI1787743, Length=471, Percent_Identity=49.0445859872611, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51681; Mature: 51550

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNTVSQGSGRKGRVLTLWTPEDKSFWAREGEAVAKINLWISVPALFLAFAIWQVWSVV
CCCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHH
AVNLPALGFKYSTNQLFWLAAAPALSGATLRIFYSFMVPIFGGRRWTALSTASLLVPAIG
HHCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
IGYAVQDNTTSYPTMLLLALLCGLGGGNFSSSMANISFFFPKERKGSALGVNAGLGNLGV
CCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHH
SVVQFLSPLVITAGIFGIFGGEPQTIVKNGQEQMVWAQNAAFIWVPWIALAAVAAWFGMN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHEEEECCCCEEEHHHHHHHHHHHHHCCC
DIADARASFAAQAAIFKRKHNWIMCVLYLGTFGSFIGFAAGFPLLIKALFPEVNPLAYAW
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHH
LGPLVGAVVRPFGGWLADKVGGARVTVWNFLVMALAVVGVLFFLPKGSGGFALPFGPAEG
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
SFTGFFLMFLVLFFTTGIGNGSTFRMIPVIFLAQKLRELRTDDEASRAQAIKDGNTEGAA
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCH
AVGFAGALGAYGGFFIPKSYGSSIAATGGPEFALWMFAVFYVLCIIITWWYYSRKHAEMP
HHHHHHHHHHCCCEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
C
C
>Mature Secondary Structure 
STNTVSQGSGRKGRVLTLWTPEDKSFWAREGEAVAKINLWISVPALFLAFAIWQVWSVV
CCCCCCCCCCCCCCEEEEECCCCCCHHHCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHH
AVNLPALGFKYSTNQLFWLAAAPALSGATLRIFYSFMVPIFGGRRWTALSTASLLVPAIG
HHCCCCCCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHH
IGYAVQDNTTSYPTMLLLALLCGLGGGNFSSSMANISFFFPKERKGSALGVNAGLGNLGV
CCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCEEEECCCCCHHHH
SVVQFLSPLVITAGIFGIFGGEPQTIVKNGQEQMVWAQNAAFIWVPWIALAAVAAWFGMN
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHEEEECCCCEEEHHHHHHHHHHHHHCCC
DIADARASFAAQAAIFKRKHNWIMCVLYLGTFGSFIGFAAGFPLLIKALFPEVNPLAYAW
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHCCCCCHHHHHH
LGPLVGAVVRPFGGWLADKVGGARVTVWNFLVMALAVVGVLFFLPKGSGGFALPFGPAEG
HHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
SFTGFFLMFLVLFFTTGIGNGSTFRMIPVIFLAQKLRELRTDDEASRAQAIKDGNTEGAA
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCH
AVGFAGALGAYGGFFIPKSYGSSIAATGGPEFALWMFAVFYVLCIIITWWYYSRKHAEMP
HHHHHHHHHHCCCEECCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]