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The map label for this gene is narK [H]

Identifier: 222109645

GI number: 222109645

Start: 434323

End: 435630

Strand: Direct

Name: narK [H]

Synonym: Dtpsy_0425

Alternate gene names: 222109645

Gene position: 434323-435630 (Clockwise)

Preceding gene: 222109644

Following gene: 222109646

Centisome position: 11.44

GC content: 63.38

Gene sequence:

>1308_bases
ATGGCTCAACTGCCCCAGCGCGCCGCGCCGAGCGCCTCAACCATCGGCGAAGATCCACGCCATACCCGCAAAGCCTGGTC
AGTGCTCATCGTCAGCACGCTCGCCTTCACCGTCTGCTTCATGGTCTGGATGATGTTCGGCGTCATCGGGATCCCGATCA
AGAAGATGCTGAACCTCAGCTCCACCCAGTTCGGTCTGCTCACGGCCATGCCCGTGCTCACGGGCTCGCTGGTGCGCGTG
CCGCTGGGTATCTGGACCGACCGCTACGGCGGCCGCATCGTGATGGCGTTGCTCATGGCCGCCACCGTTCCCGCCATCTG
GATGATGGGTTACGCCACCGAATACTGGCATTTCCTCGTGATCGGCCTGTTCGTCGGTTTGGCCGGCGGCTCGTTCTCGG
TCGGTACGCCCTACGTGGCCCGGTGGTTCCCCAAGCACCGCCAGGGCACGGCCATGGGCGTGTATGGCGCGGGCAACTCG
GGCGCGGCCGTGAACAAGTTCGTGGCGCCCGTGATCCTGGTGGCTTTCGGGTGGGCTGCCGTGCCGCATGTGTATGCCGC
CATCATGCTCGGTACGCTGGTGCTGTTCTGGCTGTTCAGCCACAGCGACCCGGCACACCTGGTGCCCAGCAACGTGTCCT
TTGGCGAACAGCTCAAGGCACTGAAGGACCCCAAGGTGCTCAAGTACTGCCAGTACTACAGCATCGTGTTCGGTGGTTAT
GTGGCGCTTTCCCTGTGGATGGTGCAGTACTACGTCGGTGAGTACGGCCTCGACATCCGTGTGGCGGCGCTGCTGGCAGC
CTGCTTCTCGCTGCCGGGTGGCGTGCTGCGCGCCATCGGCGGCGTGCTGTCCGACAAGTTCGGGGCGCACTCCGTGACCT
GGTGGGTGATGTGGGTGAGCTGGATCTGCCTGTTCCTGCTGTCCTACCCGCAGACCGACTTCACCATCCAGACCGTCAAC
GGACCCACCACCTTGCACGTCGGCTTGAACGTATACATGTTCACCGCGCTGATGTTCGTCCTGGGCATCGCCTGGGCTTT
CGGCAAGGCCAGCGTGTTCAAGTACATCAGCGACGACTACCCCAAGAACATCGGCGCCATCAGCGGCATCGTGGGCCTGG
CGGGCGGCATGGGCGGCTTCATCCTGCCCATCCTGTTCGGCATGCTCATGGACTGGACGGGCATCCGCTCCAGTGCCTTC
ATGCTGATGTACGGCGTGGTATGGGTTTCGTTGATCTGGATGTATTTCACCGAGGTGCGCCGCACCGACGTGATGAAGTC
TCACAACGCCGCCCCGGCTGCCCACTGA

Upstream 100 bases:

>100_bases
GCCCTCGTCCTTTTGCACCGCCCGCGGTGTCCCAGCGAAGGATGTGCAGGCACGGCCAGTTGCGGGACCATTGCACCGTA
CCCAAGAGAGGACATCTGCA

Downstream 100 bases:

>100_bases
TCCCCACCGGAGATCCCACATGTCCACCAACACCGTTTCGCAAGGCAGCGGCCGCAAAGGCCGGGTGCTCACGCTCTGGA
CCCCCGAGGACAAGTCCTTC

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MAQLPQRAAPSASTIGEDPRHTRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLSSTQFGLLTAMPVLTGSLVRV
PLGIWTDRYGGRIVMALLMAATVPAIWMMGYATEYWHFLVIGLFVGLAGGSFSVGTPYVARWFPKHRQGTAMGVYGAGNS
GAAVNKFVAPVILVAFGWAAVPHVYAAIMLGTLVLFWLFSHSDPAHLVPSNVSFGEQLKALKDPKVLKYCQYYSIVFGGY
VALSLWMVQYYVGEYGLDIRVAALLAACFSLPGGVLRAIGGVLSDKFGAHSVTWWVMWVSWICLFLLSYPQTDFTIQTVN
GPTTLHVGLNVYMFTALMFVLGIAWAFGKASVFKYISDDYPKNIGAISGIVGLAGGMGGFILPILFGMLMDWTGIRSSAF
MLMYGVVWVSLIWMYFTEVRRTDVMKSHNAAPAAH

Sequences:

>Translated_435_residues
MAQLPQRAAPSASTIGEDPRHTRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLSSTQFGLLTAMPVLTGSLVRV
PLGIWTDRYGGRIVMALLMAATVPAIWMMGYATEYWHFLVIGLFVGLAGGSFSVGTPYVARWFPKHRQGTAMGVYGAGNS
GAAVNKFVAPVILVAFGWAAVPHVYAAIMLGTLVLFWLFSHSDPAHLVPSNVSFGEQLKALKDPKVLKYCQYYSIVFGGY
VALSLWMVQYYVGEYGLDIRVAALLAACFSLPGGVLRAIGGVLSDKFGAHSVTWWVMWVSWICLFLLSYPQTDFTIQTVN
GPTTLHVGLNVYMFTALMFVLGIAWAFGKASVFKYISDDYPKNIGAISGIVGLAGGMGGFILPILFGMLMDWTGIRSSAF
MLMYGVVWVSLIWMYFTEVRRTDVMKSHNAAPAAH
>Mature_434_residues
AQLPQRAAPSASTIGEDPRHTRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLSSTQFGLLTAMPVLTGSLVRVP
LGIWTDRYGGRIVMALLMAATVPAIWMMGYATEYWHFLVIGLFVGLAGGSFSVGTPYVARWFPKHRQGTAMGVYGAGNSG
AAVNKFVAPVILVAFGWAAVPHVYAAIMLGTLVLFWLFSHSDPAHLVPSNVSFGEQLKALKDPKVLKYCQYYSIVFGGYV
ALSLWMVQYYVGEYGLDIRVAALLAACFSLPGGVLRAIGGVLSDKFGAHSVTWWVMWVSWICLFLLSYPQTDFTIQTVNG
PTTLHVGLNVYMFTALMFVLGIAWAFGKASVFKYISDDYPKNIGAISGIVGLAGGMGGFILPILFGMLMDWTGIRSSAFM
LMYGVVWVSLIWMYFTEVRRTDVMKSHNAAPAAH

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=442, Percent_Identity=24.4343891402715, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1787475, Length=445, Percent_Identity=26.5168539325843, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47597; Mature: 47466

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHCCCCCCCCC
>Mature Secondary Structure 
AQLPQRAAPSASTIGEDPRHTRKAWSVLIVSTLAFTVCFMVWMMFGVIGIPIKKMLNLS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCC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HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]