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The map label for this gene is trpE [H]

Identifier: 222109576

GI number: 222109576

Start: 366445

End: 367944

Strand: Direct

Name: trpE [H]

Synonym: Dtpsy_0356

Alternate gene names: 222109576

Gene position: 366445-367944 (Clockwise)

Preceding gene: 222109572

Following gene: 222109577

Centisome position: 9.65

GC content: 66.6

Gene sequence:

>1500_bases
GTGATCACTGAACTGGAATTCAAAAGCCTGGCCAGCGAAGGCTACAACCGCATCCCGCTGATGGCGGAAGCCTTTGCGGA
CCTGGAAACCCCTCTCTCCCTGTACCTCAAGCTGGCCCATGGCAAGGGCGACGGCAAGCACAGCTTTTTGCTGGAGTCGG
TCGTCGGCGGCGAGCGCTTCGGGCGCTACAGCTTCATCGGCCTGCCTGCGCGCACCTTGCTGCGGTCCAGCGGCTTCGGC
GATGCGGCGCGCACCGAGGTGGTGACCGACGGCCAAGTGGTGGAGACGCACCCGGGCAACCCGCTGGACTTCATTGCCGC
CTACCAAAAGCGCTTCAAGGTGGCGCTGCGCCCGGGGCTGCCGCGCTTTTGTGGCGGGCTGGCGGGCTACTTCGGCTACG
ACGCGGTGCGCTATATCGAGAAGAAGCTAGAGGCCACCTGCCCGCCCGACACCCTGGGCTGCCCCGACATCCTGCTGCTG
CAGTGCGAGGAGCTGGCGGTCATCGACAACCTCTCGGGCAAGCTGTATCTCATCGTCTACGCCGACCCGGCCCAGCCCGA
GGCCTATGCCAAGGGCAAGAAGCGCCTGCGGGAGCTGAAGGACCAGCTCAAGTATTCCGTGAGCGCGCCCGTGGTGCGGC
CCACCGAGAGCCACACCGCACAGCGCAGCTTTGCCAAGGCCGACTACTTGGCGGCCGTGGAGCGCGCCAAGGAGCTCATC
GCCGCGGGCGACTTCATGCAGGTGCAGGTGGGCCAGCGCATCCACAAGCGCTACACCGAATCGCCGCTGTCGCTGTACCG
CGCGCTGCGCTCGCTGAACCCGTCGCCGTACATGTATTACTACAACTTCGGCGATTTCCAGGTGGTGGGCTCCAGCCCAG
AGATCCTGGTGCGCCAGGAGAGCACGCCTGAGGGGCAGAAGGTCACCATCCGCCCGCTGGCCGGCACGCGCCCGCGCGGC
GCCACGCCCGAGGCCGACAAGGCCACCGAAGTGGAGCTGGTGAACGACCCCAAGGAACGCGCCGAGCACGTGATGCTGAT
CGACCTGGCGCGCAACGACATCGGCCGCATCGCCAAGACCGGCACCGTGAAGGTGACCGAGGCCTTCGCGGTCGAGCGCT
ACAGCCACGTGATGCACATCGTGAGCAACGTGGAGGGCATCCTCAACGACGGCATGACCAGCATGGACGTGCTCAAGGCC
ACCTTCCCGGCCGGCACGCTCACCGGCGCGCCCAAGGTGCACGCCATGGAACTGATCGACCAGTTGGAGCCCAGCAAGCG
GGGCCTTTACGGAGGCGCCTGCGGCTACCTGAGCTATGCGGGCGACATGGATGTGGCCATCGCCATCCGCACCGGCATCG
TCAAGGACGGCACGCTCTACGTGCAGGCGGCGGCCGGCGTGGTGGCCGACTCGGTGCCCGAGCTCGAATGGAAGGAAACG
GAACACAAGGCGCGCGCCCTGCTGCGTGCGGCCGAATTGGTGGAGGAGGGGCTGGAATGA

Upstream 100 bases:

>100_bases
GCCCCACACGTGCTGTCCTGCACGGGGCCGACTCCTCTGAATGATCCTGCGGCGCACTGGCGCAGCCAGCGACCGCGCCG
CACGAGCTGGGAATCTTCTT

Downstream 100 bases:

>100_bases
CCACCCGTGCAATCGACAAGACCCAGCCCTGCGCCAGCATGGCCGAGGTGCGCGCGCGCATCGACGCGCTGGACGACATC
CTGGTGCCGCTGCTCGTGGA

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MITELEFKSLASEGYNRIPLMAEAFADLETPLSLYLKLAHGKGDGKHSFLLESVVGGERFGRYSFIGLPARTLLRSSGFG
DAARTEVVTDGQVVETHPGNPLDFIAAYQKRFKVALRPGLPRFCGGLAGYFGYDAVRYIEKKLEATCPPDTLGCPDILLL
QCEELAVIDNLSGKLYLIVYADPAQPEAYAKGKKRLRELKDQLKYSVSAPVVRPTESHTAQRSFAKADYLAAVERAKELI
AAGDFMQVQVGQRIHKRYTESPLSLYRALRSLNPSPYMYYYNFGDFQVVGSSPEILVRQESTPEGQKVTIRPLAGTRPRG
ATPEADKATEVELVNDPKERAEHVMLIDLARNDIGRIAKTGTVKVTEAFAVERYSHVMHIVSNVEGILNDGMTSMDVLKA
TFPAGTLTGAPKVHAMELIDQLEPSKRGLYGGACGYLSYAGDMDVAIAIRTGIVKDGTLYVQAAAGVVADSVPELEWKET
EHKARALLRAAELVEEGLE

Sequences:

>Translated_499_residues
MITELEFKSLASEGYNRIPLMAEAFADLETPLSLYLKLAHGKGDGKHSFLLESVVGGERFGRYSFIGLPARTLLRSSGFG
DAARTEVVTDGQVVETHPGNPLDFIAAYQKRFKVALRPGLPRFCGGLAGYFGYDAVRYIEKKLEATCPPDTLGCPDILLL
QCEELAVIDNLSGKLYLIVYADPAQPEAYAKGKKRLRELKDQLKYSVSAPVVRPTESHTAQRSFAKADYLAAVERAKELI
AAGDFMQVQVGQRIHKRYTESPLSLYRALRSLNPSPYMYYYNFGDFQVVGSSPEILVRQESTPEGQKVTIRPLAGTRPRG
ATPEADKATEVELVNDPKERAEHVMLIDLARNDIGRIAKTGTVKVTEAFAVERYSHVMHIVSNVEGILNDGMTSMDVLKA
TFPAGTLTGAPKVHAMELIDQLEPSKRGLYGGACGYLSYAGDMDVAIAIRTGIVKDGTLYVQAAAGVVADSVPELEWKET
EHKARALLRAAELVEEGLE
>Mature_499_residues
MITELEFKSLASEGYNRIPLMAEAFADLETPLSLYLKLAHGKGDGKHSFLLESVVGGERFGRYSFIGLPARTLLRSSGFG
DAARTEVVTDGQVVETHPGNPLDFIAAYQKRFKVALRPGLPRFCGGLAGYFGYDAVRYIEKKLEATCPPDTLGCPDILLL
QCEELAVIDNLSGKLYLIVYADPAQPEAYAKGKKRLRELKDQLKYSVSAPVVRPTESHTAQRSFAKADYLAAVERAKELI
AAGDFMQVQVGQRIHKRYTESPLSLYRALRSLNPSPYMYYYNFGDFQVVGSSPEILVRQESTPEGQKVTIRPLAGTRPRG
ATPEADKATEVELVNDPKERAEHVMLIDLARNDIGRIAKTGTVKVTEAFAVERYSHVMHIVSNVEGILNDGMTSMDVLKA
TFPAGTLTGAPKVHAMELIDQLEPSKRGLYGGACGYLSYAGDMDVAIAIRTGIVKDGTLYVQAAAGVVADSVPELEWKET
EHKARALLRAAELVEEGLE

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=380, Percent_Identity=38.9473684210526, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI1788114, Length=450, Percent_Identity=30.8888888888889, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786809, Length=225, Percent_Identity=27.5555555555556, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI87082077, Length=272, Percent_Identity=28.3088235294118, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320935, Length=479, Percent_Identity=38.6221294363257, Blast_Score=299, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6324361, Length=471, Percent_Identity=26.963906581741, Blast_Score=132, Evalue=2e-31,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 54695; Mature: 54695

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITELEFKSLASEGYNRIPLMAEAFADLETPLSLYLKLAHGKGDGKHSFLLESVVGGERF
CCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHCCHHC
GRYSFIGLPARTLLRSSGFGDAARTEVVTDGQVVETHPGNPLDFIAAYQKRFKVALRPGL
CCEEEECCCHHHHHHCCCCCCHHHCEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCC
PRFCGGLAGYFGYDAVRYIEKKLEATCPPDTLGCPDILLLQCEELAVIDNLSGKLYLIVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHEEECCCCCEEEEEE
ADPAQPEAYAKGKKRLRELKDQLKYSVSAPVVRPTESHTAQRSFAKADYLAAVERAKELI
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGDFMQVQVGQRIHKRYTESPLSLYRALRSLNPSPYMYYYNFGDFQVVGSSPEILVRQE
HCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEEC
STPEGQKVTIRPLAGTRPRGATPEADKATEVELVNDPKERAEHVMLIDLARNDIGRIAKT
CCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHCCEEEEEEEHHHHHHHHHCC
GTVKVTEAFAVERYSHVMHIVSNVEGILNDGMTSMDVLKATFPAGTLTGAPKVHAMELID
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QLEPSKRGLYGGACGYLSYAGDMDVAIAIRTGIVKDGTLYVQAAAGVVADSVPELEWKET
HCCCCCCCCCCCCHHHHEECCCCCEEEEEEECEEECCEEEEEECCCHHHCCCCCCCHHHH
EHKARALLRAAELVEEGLE
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MITELEFKSLASEGYNRIPLMAEAFADLETPLSLYLKLAHGKGDGKHSFLLESVVGGERF
CCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCHHHEEEEECCCCCCHHHHHHHHHHCCHHC
GRYSFIGLPARTLLRSSGFGDAARTEVVTDGQVVETHPGNPLDFIAAYQKRFKVALRPGL
CCEEEECCCHHHHHHCCCCCCHHHCEEECCCEEEECCCCCCHHHHHHHHHHHHHHCCCCC
PRFCGGLAGYFGYDAVRYIEKKLEATCPPDTLGCPDILLLQCEELAVIDNLSGKLYLIVY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHEEECCCCCEEEEEE
ADPAQPEAYAKGKKRLRELKDQLKYSVSAPVVRPTESHTAQRSFAKADYLAAVERAKELI
ECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGDFMQVQVGQRIHKRYTESPLSLYRALRSLNPSPYMYYYNFGDFQVVGSSPEILVRQE
HCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCEEEECCCCCEEEEEC
STPEGQKVTIRPLAGTRPRGATPEADKATEVELVNDPKERAEHVMLIDLARNDIGRIAKT
CCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCHHHCCEEEEEEEHHHHHHHHHCC
GTVKVTEAFAVERYSHVMHIVSNVEGILNDGMTSMDVLKATFPAGTLTGAPKVHAMELID
CEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QLEPSKRGLYGGACGYLSYAGDMDVAIAIRTGIVKDGTLYVQAAAGVVADSVPELEWKET
HCCCCCCCCCCCCHHHHEECCCCCEEEEEEECEEECCEEEEEECCCHHHCCCCCCCHHHH
EHKARALLRAAELVEEGLE
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10417653 [H]