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The map label for this gene is ilvE [H]

Identifier: 222109422

GI number: 222109422

Start: 222681

End: 223619

Strand: Direct

Name: ilvE [H]

Synonym: Dtpsy_0201

Alternate gene names: 222109422

Gene position: 222681-223619 (Clockwise)

Preceding gene: 222109421

Following gene: 222109423

Centisome position: 5.87

GC content: 63.37

Gene sequence:

>939_bases
ATGAGCCCCGTTGTTCCCTCCATGGCCGACCGTGACGGCAAGATCTGGATGGATGGCCAGATGGTGGATTGGCGCGACGC
CAAGATCCACGTGCTGACCCATTCGCTGCACTACGGCTGCGGCGCCTTCGAAGGCGTGCGCGCCTACAAGACGGAGCAGG
GCACGGCCATCTTCCGCCTGGAAGAGCACACCAACCGCCTGTTCAACAGCGCCAAGATCCTGCGCATGACGATCCCCTTC
ACCAAGGACGAGGTGAATGAGGCACAGAAGGCCGTGGTGCGCGAAAACCAGCTCGAATCCTGCTACCTGCGTCCGCTGAC
GTGGATCGGCTCGGAAAAACTGGGCGTCTCGCCCAAGGGCAACAAGATTCACCTGATCGTCGCCGCCTGGGCCTGGGGCG
CGTACCTGGGCGAGGAAGGCATGCAGCGCGGCATCCGCGTCAAGACATCAAGCTTCACGCGCCACCACGTCAACATCACG
ATGACGCAGGCCAAGGCCTCGAGCAACTACACCAACTCCATCCTGGCCAACACGGAGGCCACCGACGACGGCTACGACGA
GGCATTGTTGCTGGACAACGCCGGCTTCGTCTCCGAGGGCTCGGGCGAGAACGTGTTCATCATCAAGGACGGCGTGGTCT
ACACGCCCGACCTGTCGGCCGGCGCACTGAACGGCATCACGCGCAACACCGTGCTGCATATCTGCAAGGACCTGGGGCTG
GAGGTGGTGCAAAAGCGCATCACGCGCGACGAGGTGTACATCGCCGACGAGGCCTTCTTCAGCGGCACGGCCGCCGAGAT
CACCCCGATCCGCGAACTGGACCGCGTGCAGATCGGCAGCGGCAGCCGCGGCCCGCTGACCGAGAAGATCCAATCGGCCT
TCTTCGACATCGTGAACGGCCGCAATCCCAAGTACGCCCACTGGCTCACCAAGGTCTGA

Upstream 100 bases:

>100_bases
TCGCAGGAGGACGCCGGCCCGCCTGCCGCCGCCAACCCGGCGGACGAATCGTAGAATTGCCTGCTTTGCGACCCACGCAG
TTCTCTCGAAAAGGATCTCC

Downstream 100 bases:

>100_bases
TCGCCCATGTCGCAAGCAATCGTCGAACTGCTGGCCCAGGACCTCAATCCCCAGGGCGGCGTGTACTGCCCTTCGCCCAA
GGCGGACATGAAGCTCTGGA

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MSPVVPSMADRDGKIWMDGQMVDWRDAKIHVLTHSLHYGCGAFEGVRAYKTEQGTAIFRLEEHTNRLFNSAKILRMTIPF
TKDEVNEAQKAVVRENQLESCYLRPLTWIGSEKLGVSPKGNKIHLIVAAWAWGAYLGEEGMQRGIRVKTSSFTRHHVNIT
MTQAKASSNYTNSILANTEATDDGYDEALLLDNAGFVSEGSGENVFIIKDGVVYTPDLSAGALNGITRNTVLHICKDLGL
EVVQKRITRDEVYIADEAFFSGTAAEITPIRELDRVQIGSGSRGPLTEKIQSAFFDIVNGRNPKYAHWLTKV

Sequences:

>Translated_312_residues
MSPVVPSMADRDGKIWMDGQMVDWRDAKIHVLTHSLHYGCGAFEGVRAYKTEQGTAIFRLEEHTNRLFNSAKILRMTIPF
TKDEVNEAQKAVVRENQLESCYLRPLTWIGSEKLGVSPKGNKIHLIVAAWAWGAYLGEEGMQRGIRVKTSSFTRHHVNIT
MTQAKASSNYTNSILANTEATDDGYDEALLLDNAGFVSEGSGENVFIIKDGVVYTPDLSAGALNGITRNTVLHICKDLGL
EVVQKRITRDEVYIADEAFFSGTAAEITPIRELDRVQIGSGSRGPLTEKIQSAFFDIVNGRNPKYAHWLTKV
>Mature_311_residues
SPVVPSMADRDGKIWMDGQMVDWRDAKIHVLTHSLHYGCGAFEGVRAYKTEQGTAIFRLEEHTNRLFNSAKILRMTIPFT
KDEVNEAQKAVVRENQLESCYLRPLTWIGSEKLGVSPKGNKIHLIVAAWAWGAYLGEEGMQRGIRVKTSSFTRHHVNITM
TQAKASSNYTNSILANTEATDDGYDEALLLDNAGFVSEGSGENVFIIKDGVVYTPDLSAGALNGITRNTVLHICKDLGLE
VVQKRITRDEVYIADEAFFSGTAAEITPIRELDRVQIGSGSRGPLTEKIQSAFFDIVNGRNPKYAHWLTKV

Specific function: Acts on leucine, isoleucine and valine [H]

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI38176287, Length=314, Percent_Identity=28.0254777070064, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI296010906, Length=314, Percent_Identity=28.0254777070064, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI296010904, Length=314, Percent_Identity=28.0254777070064, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI50658084, Length=321, Percent_Identity=27.4143302180685, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI48994963, Length=299, Percent_Identity=46.4882943143813, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17565728, Length=326, Percent_Identity=25.4601226993865, Blast_Score=71, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322002, Length=311, Percent_Identity=27.6527331189711, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322608, Length=288, Percent_Identity=26.3888888888889, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005785 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 34665; Mature: 34533

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPVVPSMADRDGKIWMDGQMVDWRDAKIHVLTHSLHYGCGAFEGVRAYKTEQGTAIFRL
CCCCCCCCCCCCCEEEECCEEECCCCCEEEEEEEEHHCCCCCCCCHHHEECCCCCEEEEE
EEHTNRLFNSAKILRMTIPFTKDEVNEAQKAVVRENQLESCYLRPLTWIGSEKLGVSPKG
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHEECEEEECCCCCCCCCCCC
NKIHLIVAAWAWGAYLGEEGMQRGIRVKTSSFTRHHVNITMTQAKASSNYTNSILANTEA
CEEEEEEEEEHHHHHHCHHHHHCCEEEEECCCEEEEEEEEEEECCCCCCCCCEEEECCCC
TDDGYDEALLLDNAGFVSEGSGENVFIIKDGVVYTPDLSAGALNGITRNTVLHICKDLGL
CCCCCCCEEEEECCCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHCCH
EVVQKRITRDEVYIADEAFFSGTAAEITPIRELDRVQIGSGSRGPLTEKIQSAFFDIVNG
HHHHHHHCCCEEEEECHHHHCCCCCEECCHHHHCEEEECCCCCCCHHHHHHHHHHHHHCC
RNPKYAHWLTKV
CCCCHHHHEECC
>Mature Secondary Structure 
SPVVPSMADRDGKIWMDGQMVDWRDAKIHVLTHSLHYGCGAFEGVRAYKTEQGTAIFRL
CCCCCCCCCCCCEEEECCEEECCCCCEEEEEEEEHHCCCCCCCCHHHEECCCCCEEEEE
EEHTNRLFNSAKILRMTIPFTKDEVNEAQKAVVRENQLESCYLRPLTWIGSEKLGVSPKG
HHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHEECEEEECCCCCCCCCCCC
NKIHLIVAAWAWGAYLGEEGMQRGIRVKTSSFTRHHVNITMTQAKASSNYTNSILANTEA
CEEEEEEEEEHHHHHHCHHHHHCCEEEEECCCEEEEEEEEEEECCCCCCCCCEEEECCCC
TDDGYDEALLLDNAGFVSEGSGENVFIIKDGVVYTPDLSAGALNGITRNTVLHICKDLGL
CCCCCCCEEEEECCCCCCCCCCCEEEEEECCEEECCCCCCCCCCCCHHHHHHHHHHHCCH
EVVQKRITRDEVYIADEAFFSGTAAEITPIRELDRVQIGSGSRGPLTEKIQSAFFDIVNG
HHHHHHHCCCEEEEECHHHHCCCCCEECCHHHHCEEEECCCCCCCHHHHHHHHHHHHHCC
RNPKYAHWLTKV
CCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]