Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 222109420

GI number: 222109420

Start: 220742

End: 222124

Strand: Direct

Name: radA [H]

Synonym: Dtpsy_0199

Alternate gene names: 222109420

Gene position: 220742-222124 (Clockwise)

Preceding gene: 222109417

Following gene: 222109421

Centisome position: 5.81

GC content: 69.56

Gene sequence:

>1383_bases
ATGGCCAAAGAAAAAACCATCTTCTCCTGCACCGAATGCGGTGGCACCAGTCCGCGCTGGCTGGGCAAATGCCCTTCCTG
CGGTGCGTGGAACACCCTGATCGAATCCGTGCCCGACGCTGGACCGGGCAAGAACCGCCTGAGCGGGGCCGGCCAGTACG
CGGGCCTGGCCCAGGCGCAGGCGGTGATGCCGCTGGCCGCCATCGAGGCGACCGAGGTGGCGCGCACTGCGAGCGGCATC
GAGGAACTGGACCGCGTGCTGGGCGGTGGCGTGGTCGAGGGCGGAGTGGTGCTCATCGGCGGCGACCCGGGCATCGGCAA
GTCCACGCTGCTCTTGCAGGCGATGGACGCGCTGCAGCGCGCGGGCCTGCCCACGCTGTACGTGACGGGCGAGGAGAGCG
GCGCCCAGGTGGCGTTGCGTTCGCGCCGGCTGGGGCTGGATGCGAGCCAGGTGAACGTGTTGGCCGAGATCCAACTCGAA
AAAATCCTCGCCACCGTGGAGGCCACGCAGCCCGCGGTGTGCGTGATCGACTCCATCCAGACCGTCTATTCCGACCAGCT
CACCAGCGCACCGGGCTCGGTGGCCCAGGTGCGCGAGTGCGCAGCCCACCTCACGCGCATGGCCAAGGCCACGGGCATCG
CCGTCATCCTGGTGGGCCACGTGACCAAGGAAGGTGCGCTGGCCGGCCCGCGCGTGCTGGAGCACATGGTGGACACGGTG
CTGTACTTCGAGGGCGACACGCACAGCAGCTTCCGTCTGGTGCGCGCCATCAAGAACCGCTTTGGCGCCGTGAACGAGAT
CGGCGTGTTCGCCATGACCGAGAAGGGCCTCAAGGGCGTGGCGAACCCTAGTGCCATTTTCTTGAGCCAGCACAGCGAGC
CGGTGCCCGGCAGCTGCGTGCTGGTCACGCTGGAGGGCACGCGGCCGATGCTGGTGGAGATCCAGGCGCTGGTGGACGGC
GGGGGGCCCAGCCCGCGGCGCCTGTCGGTGGGGCTGGAGCGCGACCGCCTGGCCATGCTGCTGGCCGTGCTGCACCGCCA
CGCGGGCGTGGCCTGCGCCGACCAGGATGTGTTCGTCAATGCCGTCGGGGGTGTGCGCATCAGCGAACCCGCGGCCGACC
TGGCCGTGATGCTGGCCATCACCAGCAGCCTGCGCGGCAAGGCGTTGCCCAAGGGCTTCATCGCGTTTGGCGAGGTGGGC
CTGGCTGGCGAGGTGCGCCCTGCGCCGCGTGGCCAGGAGCGCCTGAAGGAAGCCGCCAAGCTGGGCTTCACGGTGGCCGT
GGTGCCCAAAGCCAATGCGCCCAAGAAGCCGATCGCCGGGTTGGAGATCCACGCAGTGGAGCGCGTGGACGAGGCCATGA
CTGCCGTGCGCGGGCTGGACTGA

Upstream 100 bases:

>100_bases
TTCCGGAGTCGGTGTTCGCCCGGCGGGGCGGCACTGCCCGTTAGGATAGGACAGATCCCCCGCGCCGGCCCACCGCCGGC
CTACGTTGCGTCCTGCCACC

Downstream 100 bases:

>100_bases
CGCGTCGTGTCGCAGGGGCTACGCAGCTTCCACAGCGGCGCTGGTGGTGCTGCGTAGGACAATGCGGCCATGAATCTGCG
CAAGATATTGATCCCAGCGG

Product: DNA repair protein Rada

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MAKEKTIFSCTECGGTSPRWLGKCPSCGAWNTLIESVPDAGPGKNRLSGAGQYAGLAQAQAVMPLAAIEATEVARTASGI
EELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALQRAGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQLE
KILATVEATQPAVCVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRMAKATGIAVILVGHVTKEGALAGPRVLEHMVDTV
LYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVDG
GGPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVNAVGGVRISEPAADLAVMLAITSSLRGKALPKGFIAFGEVG
LAGEVRPAPRGQERLKEAAKLGFTVAVVPKANAPKKPIAGLEIHAVERVDEAMTAVRGLD

Sequences:

>Translated_460_residues
MAKEKTIFSCTECGGTSPRWLGKCPSCGAWNTLIESVPDAGPGKNRLSGAGQYAGLAQAQAVMPLAAIEATEVARTASGI
EELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALQRAGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQLE
KILATVEATQPAVCVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRMAKATGIAVILVGHVTKEGALAGPRVLEHMVDTV
LYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVDG
GGPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVNAVGGVRISEPAADLAVMLAITSSLRGKALPKGFIAFGEVG
LAGEVRPAPRGQERLKEAAKLGFTVAVVPKANAPKKPIAGLEIHAVERVDEAMTAVRGLD
>Mature_459_residues
AKEKTIFSCTECGGTSPRWLGKCPSCGAWNTLIESVPDAGPGKNRLSGAGQYAGLAQAQAVMPLAAIEATEVARTASGIE
ELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALQRAGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQLEK
ILATVEATQPAVCVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRMAKATGIAVILVGHVTKEGALAGPRVLEHMVDTVL
YFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCVLVTLEGTRPMLVEIQALVDGG
GPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVNAVGGVRISEPAADLAVMLAITSSLRGKALPKGFIAFGEVGL
AGEVRPAPRGQERLKEAAKLGFTVAVVPKANAPKKPIAGLEIHAVERVDEAMTAVRGLD

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=464, Percent_Identity=56.0344827586207, Blast_Score=501, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 47829; Mature: 47698

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKTIFSCTECGGTSPRWLGKCPSCGAWNTLIESVPDAGPGKNRLSGAGQYAGLAQAQ
CCCCCCEEEECCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
AVMPLAAIEATEVARTASGIEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHH
AGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQLEKILATVEATQPAVCVIDSIQ
CCCCEEEEECCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
TVYSDQLTSAPGSVAQVRECAAHLTRMAKATGIAVILVGHVTKEGALAGPRVLEHMVDTV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHE
LYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCV
EEECCCCCHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCEE
LVTLEGTRPMLVEIQALVDGGGPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVN
EEEECCCCCEEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEECCCCEEEE
AVGGVRISEPAADLAVMLAITSSLRGKALPKGFIAFGEVGLAGEVRPAPRGQERLKEAAK
EECCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHHHHHHH
LGFTVAVVPKANAPKKPIAGLEIHAVERVDEAMTAVRGLD
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKEKTIFSCTECGGTSPRWLGKCPSCGAWNTLIESVPDAGPGKNRLSGAGQYAGLAQAQ
CCCCCEEEECCCCCCCCHHCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHH
AVMPLAAIEATEVARTASGIEELDRVLGGGVVEGGVVLIGGDPGIGKSTLLLQAMDALQR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCHHHHHHHHHHHHHH
AGLPTLYVTGEESGAQVALRSRRLGLDASQVNVLAEIQLEKILATVEATQPAVCVIDSIQ
CCCCEEEEECCCCCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHH
TVYSDQLTSAPGSVAQVRECAAHLTRMAKATGIAVILVGHVTKEGALAGPRVLEHMVDTV
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHHHHHHHHE
LYFEGDTHSSFRLVRAIKNRFGAVNEIGVFAMTEKGLKGVANPSAIFLSQHSEPVPGSCV
EEECCCCCHHHHHHHHHHHHCCCHHHCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCEE
LVTLEGTRPMLVEIQALVDGGGPSPRRLSVGLERDRLAMLLAVLHRHAGVACADQDVFVN
EEEECCCCCEEEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCEECCCCEEEE
AVGGVRISEPAADLAVMLAITSSLRGKALPKGFIAFGEVGLAGEVRPAPRGQERLKEAAK
EECCEEECCCHHHHHHHHHHHHHHCCCCCCCCHHEECCCCCCCCCCCCCCCHHHHHHHHH
LGFTVAVVPKANAPKKPIAGLEIHAVERVDEAMTAVRGLD
CCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]